Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan最新文献

{"title":"Exploring the potential mechanism of Huang'e capsule against spontaneous benign prostatic hyperplasia in beagle dogs using high-performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry, gas chromatography-mass spectrometry, and network pharmacology.","authors":"M A Aicui, Pan Qi, Chen Dingshi, Wang Zhonghui, Zheng Chiyang, Zhang Junfang, Yan Jianyan, L I Lei, Yan Han, Yao Shunheng, Guo Jun, Sun Zuyue","doi":"10.19852/j.cnki.jtcm.2026.02.009","DOIUrl":"https://doi.org/10.19852/j.cnki.jtcm.2026.02.009","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the therapeutic efficacy and potential mechanisms of Huang'e capsule (, HEC) against benign prostatic hyperplasia (BPH).</p><p><strong>Methods: </strong>The chemical profile of HEC was characterized using high-performance liquid chromatographyquadrupole-time-of-flight tandem mass spectrometry (HPLC-Q-TOF-MS/MS) and gas chromatography-mass spectrometry (GC-MS) techniques. Network pharmacology was employed to analyze potential active compounds, core targets, and key signaling pathways. A spontaneous canine BPH model was used to evaluate the efficacy of HEC and to validate the predictions from network pharmacology.</p><p><strong>Results: </strong>A total of 51 chemical components of HEC were identified, comprising 19 from HPLC-Q-TOF-MS/MS and 32 from GC-MS analyses. The \"components-targets-pathways-disease\" network analysis predicted active compounds including (s)-coriolic acid, ethyl linoleate, peroxysimulenoline, physcion, and kaempferol. Core targets identified included cytochrome P450 family 19 subfamily A member 1, estrogen receptor 2 (ESR2), ESR1, and androgen receptor (AR). Kyoto Encyclopedia of Genes and Genomes enrichment analysis suggested that HEC's effects on BPH involve pathways related to cancer, phosphatidylinositol 3-kinase (PI3K) -protein kinase B (Akt)-signaling, proteoglycans in cancer, and prostate cancer signaling. Animal experiments showed that HEC significantly improved maximum urinary flow rates, reduced prostate weight, volume, and prostate index, and ameliorated histopathological changes. HEC regulated the balance between apoptosis and proliferation by downregulating AR and estrogen receptor alpha expression, while upregulating estrogen receptor beta expression.</p><p><strong>Conclusion: </strong>These findings indicate that HEC effectively ameliorates spontaneous BPH in beagle dogs, likely by regulating the balance between cell apoptosis and proliferation through multiple signaling pathways.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 2","pages":"360-370"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13077110/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147793272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Traditional Chinese Medicine psychosomatic integration therapy for subthreshold depression: a multicenter randomized controlled trial.","authors":"H U Wenyue, Zhong Biying, Wang Zhenyi, Zhang Meng, Chang Ze, Tan Xi, Han Zhenyun","doi":"10.19852/j.cnki.jtcm.2026.02.011","DOIUrl":"https://doi.org/10.19852/j.cnki.jtcm.2026.02.011","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the efficacy of Traditional Chinese Medicine (TCM) psychosomatic integration therapy in treating subthreshold depression (SD).</p><p><strong>Methods: </strong>A multicenter randomized controlled trial was conducted. Eligible participants were evaluated by physicians and randomly assigned to either the intervention or control group. The intervention group received group psychotherapy, <i>Jue</i> tune music therapy, and Yuleyin oral formula. The control group received only group psychotherapy. The intervention period lasted 12 weeks, followed by a 12-week follow-up. The primary outcome was the Center for Epidemiologic Studies Depression Scale (CES-D) score at week 12. Secondary outcomes included dropout rate, scores of the Hamilton Anxiety Scale (HAMA) and the Hamilton Depression Rating Scale (HAMD-17) at week 12, and CES-D scores at weeks 4, 8, 16, 20, and 24.</p><p><strong>Results: </strong>A total of 505 patients were randomly allocated to the two groups, and 496 participants [77% female; (38 ± 16) years] were included in the final analysis. The primary outcome showed no statistically significant difference in CES-D scores between the intervention and control groups at week 12 (<i>P ></i> 0.05). However, the intervention group exhibited significant reductions in CES-D scores at weeks 4, 8, 12, 16, 20, and 24 compared to baseline, and at week 24 compared to week 12 (<i>P</i> < 0.0001). The control group also showed significant reductions in CES-D scores at weeks 4 and 8 during the intervention period (<i>P</i> < 0.0001). Although no significant differences were observed between groups at each specific time point, the intervention group showed a more consistent downward trend. Additionally, both HAMD and HAMA scores significantly decreased from baseline to week 12 in both groups (<i>P</i>< 0.0001).</p><p><strong>Conclusions: </strong>TCM psychosomatic integration therapy, which includes group psychotherapy, <i>Jue</i> tune music therapy, and Yuleyin oral formula, may be effective in improving symptoms of SD, with good safety and feasibility. The complete TCM intervention demonstrated better long-term effectiveness than group psychotherapy alone. Further high-quality studies are needed to validate these findings.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 2","pages":"392-400"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13077112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147793323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xiaotan Sanjie recipe suppressed the oncogenic potential of cancer-associated fibroblaststhe stromal-derived factor 1α/C-X-C chemokine receptor type 4 pathway in gastric cancer.","authors":"Zhao Ying, Zhu Zhenxin, Y U Zhihong, Xiu Lijuan, Liu Yongying, L U Ye, Wei Pinkang","doi":"10.19852/j.cnki.jtcm.2026.02.003","DOIUrl":"https://doi.org/10.19852/j.cnki.jtcm.2026.02.003","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the impact and mechanism of action of Xiaotan Sanjie (, XTSJ) on the invasiveness and metastasis of gastric cancer (GC) by modulating cancer-associated fibroblasts (CAFs). MKN-45 cells were used in <i>in vitro</i> experiments, and an <i>in vivo</i>xenograft model was established for further analysis.</p><p><strong>Methods: </strong>Two types of fibroblasts, CAFs and normal fibroblasts, were isolated from the tissue sample of two patients following GC surgery. The <i>in vitro</i> and <i>in vivo</i>experiments were treated with XTSJ. Immun-ohistochemistry and enzyme-linked immunosorbent assay (ELISA) were used to assess levels of Vimentin, α-smooth muscle actin, and C-X-C chemokine receptor type 4 (CXCR4). The quantities of interleukin-6 (IL-6), stromal-derived factor 1α (SDF-1α), vascular endothelial growth factor (VEGF), and interleukin-1β (IL-1β) were measured using quantitative real-time polymerase chain reaction and ELISA.</p><p><strong>Results: </strong>The results indicated that CAFs significantly enhance the migration and invasion of GC cells compared to NFs. However, following treatment with XTSJ, CAF's ability to promote GC was reduced. XTSJ treatment significantly inhibited CAF proliferation and downregulated the expression of CAF-associated cytokines IL-1β, IL-6, VEGF, and SDF-1α. The SDF-1α/ CXCR4 signaling pathway is a potential key mechanism through which CAFs impact GC invasion.</p><p><strong>Conclusion: </strong>XTSJ treatment inhibited tumor-promoting capabilities in CAFs, suppressing GC progression, invasion, and metastasis. This study offers empirical support for the potential clinical application of XTSJ in treating and preventing GC metastasis.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 2","pages":"294-305"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13077105/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147793365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of Shaofu Zhuyu decoction in improving diabetic mellitus erectile dysfunction inhibition of ferroptosis based on network pharmacology and experimental validation.","authors":"Wang Zhuo, Mao Yinhui, Zang Yueyue, H E Shuangyan, Sun Juntao, Wei Zhitao, Wang Mingxing, Yang Yong","doi":"10.19852/j.cnki.jtcm.2026.02.010","DOIUrl":"https://doi.org/10.19852/j.cnki.jtcm.2026.02.010","url":null,"abstract":"<p><strong>Objective: </strong>To explore the medication patterns and mechanisms of action of Shaofu Zhuyu decoction (, SFZYD) in inhibiting ferroptosis through the nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase 1 (HO-1)/glutathione peroxidase 4 (GPX4) pathway to improve diabetes mellitus-induced erectile dysfunction (DMED).</p><p><strong>Methods: </strong>Firstly, data mining was employed to identify the medication patterns of Traditional Chinese Medicine (TCM) in treating DMED. Secondly, network pharmacology combined with a ferroptosis database was used to predict the targets. Subsequently, cell counting kit-8, 4',6-diamidino-2-phenylindole staining, reverse transcription-polymerase chain reaction (RT-PCR), and reagent kits were utilized to assess the repair effects of SFZYD on corpus cavernosum endothelial cells (CCECs) induced by high glucose (HG). Metabolic indicators, hematoxylin-eosin staining, and Masson staining were performed to observe the restorative effects of SFZYD on erectile function and penile tissue in diabetic rats. Finally, using Nrf2 inhibitors, the expression of related proteins and mRNAs was detected through Western blotting and RT-PCR. Reactive oxygen species levels and mitochondrial membrane potential were detected by flow cytometry.</p><p><strong>Results: </strong>Data mining revealed that the prescription rules for blood stasis-type DMED coincide with the treatment principles of SFZYD. Network pharmacology identified 48 ferroptosis-related targets, primarily heme oxygenase 1 (HMOX1) and GPX4. Kyoto Encyclopedia of Genes and Genomes enrichment analysis associated these targets with the ferroptosis pathway. SFZYD repaired HG-induced CCECs damage and restored HMOX1 and GPX4 mRNA levels. <i>in vivo</i>, SFZYD effectively alleviated erectile dysfunction and repaired blood sinuses and fibrosis in diabetic rats. Following Nrf2 inhibition, the expression of Nrf2, HMOX1, and GPX4 decreased, while SFZYD intervention reversed these effects, improving ferroptosis and oxidative stress indicators.</p><p><strong>Conclusion: </strong>This study explored the potential mechanisms and efficacy of the TCM prescription SFZYD in treating DMED through data mining, network pharmacology analysis, cellular experiments, and animal experiments. It verified its effectiveness in repairing HG-induced CCECs damage, improving the pathological state of penile tissue in diabetic rats, and restoring erectile function by regulating the Nrf2/HO-1/GPX4 signaling pathway. This provides new insights and scientific evidence for treating DMED with TCM.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 2","pages":"382-391"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13077106/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147793439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterizing the distribution of Chinese medicine syndrome of cancer-related fatigue in childhood lymphoma based on latent structure model.","authors":"Dong Ziqi, Huang Jing, L U Lu, Chen Yuru, Gan Guoling, Duan Yanlong, Jin Ling, Huang Shuang, Zhang Meng, L I Nan, Gao Zhen, Lü Jiaxuan","doi":"10.19852/j.cnki.jtcm.2026.02.018","DOIUrl":"https://doi.org/10.19852/j.cnki.jtcm.2026.02.018","url":null,"abstract":"<p><strong>Objective: </strong>To explore the distribution patterns of Traditional Chinese Medicine (TCM) syndromes in children with lymphoma-related cancer-related fatigue (CRF) using latent structure model analysis.</p><p><strong>Methods: </strong>This was a single-center cross-sectional study in which Chinese medicine diagnostic information was collected from 153 children diagnosed with childhood lymphoma CRF from July 2022 to December 2023. The latent structure model of the CRF for childhood lymphoma was constructed using the latent tree model-extension adjustment simplification until termination algorithm, and the population was classified using comprehensive clustering. Combined with the existing guidelines, we explored the distribution characteristics and dynamic progression of syndrome types in patients with childhood lymphoma CRF.</p><p><strong>Results: </strong>Initially, a latent structural model incorporating 72 manifest variables was constructed. Through comprehensive clustering, 6 distinct TCM syndrome types were identified, and corresponding quantitative identification rules were formulated. The 153 pediatric cases were then reclassified according to these rules, with the syndromes ranked in descending order of percentage as follows: blood deficiency and stasis, vigorous fire due to <i>Yin</i>deficiency, deficiency of both the lung and spleen, liver constraint and <i>Qi</i> stagnation, dampness-heat retention, and heart-spleen heat accumulation. Preliminary findings suggest that the shifting patterns of TCM syndromes in childhood lymphoma CRF may exhibit clinically meaningful relationships with tumor staging and treatment time courses.</p><p><strong>Conclusion: </strong>Using latent structure analysis, 6 TCM syndromes and their quantitative identification rules were identified. A comparison with existing guidelines revealed that CRF in patients with childhood lymphoma is characterized primarily by a syndrome of mixed deficiency and excess. Therefore, in the treatment of pediatric CRF, it is recommended not only that deficiency patterns be addressed but also that the clinical stage and treatment phase of the tumor be considered. Additionally, therapeutic strategies should emphasize promoting digestion, resolving food stagnation, regulating <i>Qi</i>, and clearing heat to formulate a tailored treatment plan.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 2","pages":"458-469"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13077115/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147793213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the effects of natural, cultivated, and synthetic musk on preventing acute cerebral ischemia/reperfusion injury in rats.","authors":"Liang Tianyu, Liu Weiying, Tang Huayang, Peng Fu, Peng Cheng, Pan Xiaoqi","doi":"10.19852/j.cnki.jtcm.20250924.001","DOIUrl":"10.19852/j.cnki.jtcm.20250924.001","url":null,"abstract":"<p><strong>Objective: </strong>To find alternatives to natural musk, this study compared the pharmacological effects of cultivated musk, synthetic musk, and natural musk. The aim is to address the dual challenge of clinical demand for musk-based medicines and the endangered status of the musk deer population.</p><p><strong>Methods: </strong>This study aimed to establish a rat model of acute cerebral ischemia/reperfusion injury (CI/RI) by creating a middle cerebral artery occlusion and reperfusion model. Methods including enzyme-linked immunosorbent assay kits, hematoxylin and eosin staining, Nissl staining, immunohistochemistry, and Western blot were employed to compare the extent of brain tissue damage, inflammatory factor levels, and platelet-related indicators in rats treated with musk from different sources (natural musk, cultivated musk, and synthetic musk), thereby evaluating their differences in neuroprotection. Analysis was performed using one-way analysis of variance.</p><p><strong>Results: </strong>Results indicated that pretreatment with musk demonstrated a positive impact on neurological function by reducing cerebral infarction size, decreasing cerebral edema severity, increasing calcitonin gene related peptide levels, inhibiting 5-hydroxytryptamine release, and preserving the blood-brain barrier integrity. Notably, natural musk exhibited superior antithrombotic properties compared to cultivated and synthetic musk, primarily evidenced by its ability to inhibit platelet aggregation rate, improve cerebral blood perfusion, reduce platelet activating factor receptor protein expression, and lower thromboxane A2 levels. Cultivated musk was observed to elevate catalase and superoxide dismutase levels while concurrently dampening inducible nitric oxide synthase activity, thereby mitigating oxidative damage and also diminishing tumor necrosis factor-α and interleukin-1β contents along with Glial fibrillary acidic protein expression, enhancing anti-inflammatory capacity.</p><p><strong>Conclusion: </strong>Musk sourced from diverse origins exhibits profound neuroprotective qualities against acute CI/RI in rats. Particularly, natural musk demonstrated a specific propensity towards reinforcing antithrombotic effects, whereas cultivated musk excelled in augmenting anti-inflammatory and antioxidative defenses, offering efficacious protection against acute CI/RI. The findings bolster the credibility of strategically employing diverse sources of musk as a preventive strategy against ischemic strokes.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 1","pages":"39-50"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12884503/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147286926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on intelligent identification of Traditional Chinese Medicine constitutions based on multi-model fusion.","authors":"Yue Yao, Cai Xiaohong","doi":"10.19852/j.cnki.jtcm.2026.01.022","DOIUrl":"10.19852/j.cnki.jtcm.2026.01.022","url":null,"abstract":"<p><strong>Objective: </strong>To develop an automated system for identifying and classifying constitution types in Traditional Chinese Medicine (TCM) by leveraging multi-model fusion algorithms.</p><p><strong>Methods: </strong>A condensed version of a physical information collection form was designed to facilitate efficient data acquisition. The collected data were analyzed using a multi-model fusion approach, which integrated several machine learning techniques. These included support vector machines, Naive Bayes, decision trees, random forests, logistic regression, multilayer perceptrons, K-nearest neighbors, gradient boosting, adaptive ensemble learning, and recurrent neural networks. A soft voting strategy was used to combine the predictive outputs of each model, enabling the selection of the most effective model combination.</p><p><strong>Results: </strong>The classification models demonstrated consistent and robust performance across most TCM constitution types when enhanced by the multi-model fusion strategy. In particular, high levels of accuracy, precision, recall, and F1-score were achieved for constitution types such as <i>Yang</i> deficiency, <i>Qi</i> deficiency, and <i>Qi</i> stagnation. However, the classification performance for the <i>Yin</i> deficiency constitution was relatively lower, indicating the need for further refinement and optimization in future research.</p><p><strong>Conclusion: </strong>This study introduces a novel, automated method for classifying TCM constitution types through the application of multi-model fusion algorithms. The approach simplifies the complex task of constitution identification while offering a practical and theoretical framework for the intelligent diagnosis of TCM body types. The findings have the potential to enhance personalized health management and support clinical decision-making in TCM diagnosis and treatment.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 1","pages":"226-235"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12884490/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147286975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A network pharmacology-based strategy to discover the molecular mechanism of correlation between the treatment of bronchial asthma with Wenyang Huayin decoction and autophagy.","authors":"Zhao Mingzhe, Yan Peizheng, Zhao Xiaomin, Chen Yufan, L I Mengsitong, Zhou Yuping, Zhang Lu, Dou Liwen, Liu Yan, Zheng Hong, L I Jia","doi":"10.19852/j.cnki.jtcm.2026.01.013","DOIUrl":"10.19852/j.cnki.jtcm.2026.01.013","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the key targets and mechanisms of the Wenyang Huayin decoction (WYHYD, ) in treating bronchial asthma with cold fluid retention syndrome using network pharmacology and animal experiments.</p><p><strong>Methods: </strong>On the one hand, we used network pharmacology method to explored the chemical components of the WYHYD and the main targets of bronchial asthma were acquired. Besides, a protein interaction network was built after protein interaction analysis to find potential protein functional modules. Then, we constructed the \"WYHYD component-bronchial asthma target-pathway\" network. On the other hand, the experimental intervention was as follows: first, we formed a rat model of bronchial asthma. Thereafter, we used the WYHYD to treat the disease while observing autophagy-related indicators as the core target of network pharmacology.</p><p><strong>Results: </strong>The network pharmacology revealed that there are 122 potential therapeutic targets in treating bronchial asthma with WYHYD. The biological processes mainly involved in the WYHYD included Gene Ontology: 0110032: positive regulation of G2/MI transition of the medical cell cycle etc. and four major signaling pathways were involved. During laboratory investigations, various signs and clinical manifestations of the rat model group were the same. After administering the WYHYD, lung function, pathological sections, inflammatory factors, and other microscopic indicators improved to varying degrees, providing evidence for the study results. Meanwhile, we verified that the core targets of WYHYD in treating asthma through the intervention of autophagy are tumor necrosis factor, caspase 8, interleukin 1 beta, sirtuin 1, phosphatidylinositol 3-kinase catalytic subunit type 3, C-C chemokine receptor type 7, L/YN kinase, and protein tyrosine kinase 2.</p><p><strong>Conclusion: </strong>This preliminary study revealed the treatment process of bronchial asthma with multi-component, multi-target, and multi-pathway mechanisms of the WYHYD.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 1","pages":"138-148"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12884495/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147286880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soufeng Yuchuan formula alleviates asthma airway inflammation and suppresses the progression of asthma by inhibiting ferroptosis in airway epithelial cells.","authors":"Wei Mumu, Yan Xiaoyu, Zhang Yujing, Zhang Xinxin, Yan Yongbin","doi":"10.19852/j.cnki.jtcm.2026.01.007","DOIUrl":"10.19852/j.cnki.jtcm.2026.01.007","url":null,"abstract":"<p><strong>Objective: </strong>To explore whether Soufeng Yuchuan (, SFYC) formula alleviates asthma by promoting ferroptosis of airway epithelial cells.</p><p><strong>Methods: </strong>A chronic asthma rat model was established using ovalbumin (OVA) combined with aluminum hydroxide sensitization and OVA nebulization stimulation. Lung tissue pathology was assessed <i>via</i> hematoxylin and eosin staining and periodic acid-Schiff staining. Lung tissue ultrastructure was observed using transmission electron microscopy. Serum cytokine levels were measured by enzyme-linked immunosorbent assay. Malondialdehyde (MDA), glutathione, and tissue ferrous ion content in rat lung tissues and cells were detected using commercial kits. Immunohistochemical staining was used to determine the expression levels of interleukin-13 (IL-13) and interleukin-6 (IL-6) in lung tissue. Western blotting was employed to detect the expression levels of transferrin receptor 1 (TFR1), transferrin receptor 2 (TFR2), acyl-CoA synthetase long-chain family member 4 (ACSL4), glutathione peroxidase 4 (GPX4), and solute carrier family 7 member 11 (SLC7A11). Cell proliferation, apoptosis and reactive oxygen species (ROS) were evaluated using a cell counting kit-8 assay and flow cytometry. Intracellular lipid peroxidation was detected using a C11-BODIPY 581/591 (C11-BODIPY) probe.</p><p><strong>Results: </strong>SFYC formula effectively alleviates airway inflammation in asthmatic rats in a dose-dependent manner. It improves airway epithelial cell integrity, reduces alveolar wall thickening and expansion, inhibits goblet cell proliferation and mucus secretion, and decreases the expression of IL-6 and IL-13 in lung tissue, as well as the serum levels of IL-6, IL-13, interleukin-4 (IL-4), and immunoglobulin E (IgE). SFYC formula mitigates ferroptosis in lung tissue of asthmatic rats. At the cellular level, it promotes proliferation of rat airway epithelial cells, inhibits cell apoptosis, suppresses ROS, MDA, and lipid accumulation, reduces the expression of TFR1, TFR2, and ASCL4, and increases the expression of GPX4 and SLC7A11 to mitigate ferroptosis.</p><p><strong>Conclusion: </strong>SFYC formula alleviates asthma airway inflammation by inhibiting ferroptosis in airway epithelial cells, providing new insights and potential therapeutic targets for the development of new asthma treatment drugs.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 1","pages":"73-84"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12884493/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147287025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Baitouweng Tang alleviates dextran sulfate sodium-induced ulcerative colitis in mice: a network pharmacology combined with experimental study.","authors":"Sun Yan, Zhou Haozheng, Feng Jianhui, Lin Zikai, H E Jie, Wang Zhenhua, Guo Yuting, Wen Shaohong, L I Gang","doi":"10.19852/j.cnki.jtcm.2026.01.011","DOIUrl":"10.19852/j.cnki.jtcm.2026.01.011","url":null,"abstract":"<p><strong>Objective: </strong>To explore the mechanism of Baitouweng Tang (, Pulsatilla decoction, PD) alleviates dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice by integrating network pharmacology prediction with experimental validation, focusing on the modulation of inflammatory signaling.</p><p><strong>Methods: </strong>A chronic UC model was induced in C57BL/6 mice by cyclical administration of DSS. Mice were treated with either a low (15 mL/kg) or high (30 mL/kg) dose of PD. Disease severity was assessed clinically and via histopathology. Serum levels of inflammatory cytokines were quantified. A network pharmacology approach was employed to predict the core targets and pathways of PD against UC. Key predictions concerning the toll-like receptor 4/nuclear factor-kappa B (TLR4/NF-κB) pathway were subsequently verified in colonic tissue using quantitative polymerase chain reaction and Western blotting.</p><p><strong>Results: </strong>PD treatment significantly ameliorated DSS-induced UC symptoms, including reducing disease activity, preventing colon shortening, and improving histological architecture. PD effectively rebalanced the systemic inflammatory milieu by decreasing pro-inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) and elevating anti-inflammatory cytokines interleukin-10 (IL-10). Network pharmacology analysis identified the TLR4/NF-κB signaling pathway as a central target. Experimental validation confirmed that PD markedly suppressed the upregulation of both TLR4 and NF-κB at the transcriptional and protein levels in the inflamed colon.</p><p><strong>Conclusion: </strong>PD demonstrates protective effects against experimental UC. Its mechanism is associated with the inhibition of the TLR4/NF-κB signaling pathway and the subsequent attenuation of inflammatory responses. This study provides a modern pharmacological basis for the classical application of PD in treating heat-toxin related intestinal disorders, bridging traditional use and mechanistic understanding.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 1","pages":"119-126"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12884485/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147286834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}