Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan最新文献

{"title":"Research and development of the pulse acquisition system and the pulse biomimetic reproduction system.","authors":"Y I Kai, M A Yunjing, Guo Shen, Liu Heng, X U Bin","doi":"10.19852/j.cnki.jtcm.2025.01.021","DOIUrl":"10.19852/j.cnki.jtcm.2025.01.021","url":null,"abstract":"<p><p>The paper is an introduction to the front-end pulse acquisition and the back-end pulse biomimetic reproduction system. This system is capable of faithfully replicating the complete pulse waveform collected at the front end. Traditional Chinese Medicine (TCM) practitioners analyze and diagnose the pulse patterns at the replication end. Meanwhile, the obtained pulse waveforms are analyzed and learnt by a neural network based on key diagnostic points in TCM pulse taking, which enables the determination of the corresponding relationships between different pulse waveforms and various pulse patterns in TCM pulse taking. With the support of clinical samples, an auxiliary diagnostic system for TCM pulse patterns ensures the accuracy of pulse pattern replication.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"213-220"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Difference of the gut microbiota of premature ovarian insufficiency in two traditional Chinese syndromes.","authors":"W U Jiaman, Ning Yan, Tan Liya, M A Fei, Lin Yanting, Zhuo Yuanyuan","doi":"10.19852/j.cnki.jtcm.2025.01.012","DOIUrl":"10.19852/j.cnki.jtcm.2025.01.012","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the differences in gut microbial characteristics between two traditional Chinese syndromes of premature ovarian insufficiency (POI).</p><p><strong>Methods: </strong>Forty women with POI were recruited from the Department of Traditional Chinese Medicine at Shenzhen Maternity and Child Healthcare Hospital between June and December 2020. Women with POI were divided into the kidney deficiency and blood stasis syndrome (SDBS) and <i>Qi</i> and blood deficiency syndrome (QBDS) groups. Gut microbial community profiles were analyzed by 16S rRNA gene sequencing using an Illumina MiSeq system. A retrospective study comparing hormone levels and gut microbiota information was performed between the SDBS and QBDS groups.</p><p><strong>Results: </strong>Compared with the QBDS group, the serum levels of estradiol (E2) and anti-Müllerian hormone (AMH) were significantly decreased in the SDBS group. The quantities of Adlercreutzia, Eggerthella, Klebsiella, and Paraprevotella significantly increased in the SDBS group, whereas Lactobacillus decreased significantly. Moreover, alterations in the microbiome in the SDBS and QBDS groups were closely related to the levels of E2 and AMH. The area under the receiver operating characteristic curve for the classification of the two syndromes by the gut microbiome was 0.71.</p><p><strong>Conclusions: </strong>There were significant differences in the dominant microbiota between the SDBS and QBDS groups, and the change in Proteobacteria in the QBDS group was more significant. The characteristics of gut microbiota help us differentiate between the SDBS and QBDS groups, which may provide a basis for the objectification of TCM syndrome types.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"132-139"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect and mechanism of Sanqi () in treating periodontitis.","authors":"A N Yuanyuan, Liu Wang, L I Yanjie, Wang Yanchun, Ren Xiaobin, H E Hongbing","doi":"10.19852/j.cnki.jtcm.20241111.001","DOIUrl":"10.19852/j.cnki.jtcm.20241111.001","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effect and mechanism of Sanqi (<i>Radix Notoginseng</i>) in treating periodontitis.</p><p><strong>Methods: </strong>The active components and periodontitis targets were analyzed through network pharmacology and molecular docking. A rat model of periodontitis was established and rats were treated by continuous intragastric administration of Sanqi (<i>Radix Notoginseng</i>) at different doses for 30 d. The alveolar bone structure was observed by micro-CT, the periodontal tissue structure was observed by hematoxylin-eosin staining, and the related proteins changes was detected by immunohistochemical staining.</p><p><strong>Results: </strong>Sanqi (<i>Radix Notoginseng</i>) and periodontitis had a total of 96 coincident targets that were significantly enriched in the interleukin 17 (IL-17), tumor necrosis factor (TNF), and advanced glycation endproducts and the receptor of advanced glycation endproducts signaling pathways. The active compound quercetin had good binding activity with interleukin 6 (IL-6), vascular endothelial growth factor A (VEGFA), matrix metallopeptidase 9 (MMP9), tumor necrosis factor α (TNF-α), Jun proto-oncogene (JUN), and C-X-C motif chemokine ligand 8 (CXCL8) in periodontitis. Compared with normal group, the distance from the cementoenamel junction (CEJ) to the alveolar bone (AB) was increased, alveolar bone absorption was obvious, the periodontal tissue structure was disorganized, and IL-6 and TNF-α were upregulated in periodontitis group; meanwhile, the distance from CEJ to AB was significantly decreased, alveolar bone resorption was reduced, periodontal tissue structure was improved, the expression of IL-6, TNF-α, IL-17 and retinoid-ralated orphan receptor γt (RORγt) were decreased, Forkhead Box P3 (FOXP3) and IL-10 were increased after Sanqi (<i>Radix Notoginseng</i>) treatment.</p><p><strong>Conclusions: </strong>Sanqi (<i>Radix Notoginseng</i>) improves the structure of alveolar bone and gum, and reduces inflammation; the mechanism involve in inhibiting IL-17 signaling pathway to suppress Th17 and promote Treg cells differentiation.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"66-75"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Network pharmacology and animal experiments revealed the protective effects of Guilong prescription on chronic prostatitis and its possible mechanisms.","authors":"Zhu Peixuan, S U Zeqi, Fan Qiongyin, Zhang Cai, Wang Ting","doi":"10.19852/j.cnki.jtcm.20240423.002","DOIUrl":"10.19852/j.cnki.jtcm.20240423.002","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the protective effects of Guilong prescription (, GL) on chronic prostatitis (CP) and unravel the underlying mechanisms of its pharmacological effects.</p><p><strong>Methods: </strong>The composition of GL was determined <i>via</i> linear ion trap/electrostatic field orbital trap tandem high-resolution mass spectrometry, and the identified compounds were performed network pharmacological analysis to predict possible pathways of the effects of GL on CP. A CP rat model was established by carrageenan, and rats were randomly assigned into a Control group, Sham group, CP group, GL low dose (3.5 g/kg) group, GL medium dose (7 g/kg) group, and GL high dose (14 g/kg) group. Hematoxylin-eosin staining of the prostate, and prostate blood -perfusion measured by laser speckle contrast analysis were used to evaluate the efficacy of GL. Expression of intercellular cell adhesion molecule-1 (ICAM-1) and induce nitric oxide synthase (iNOS) were determined by immunohistochemistry, and the content of interferon-γ (IFN-γ), interleukin-1β (IL-1β), interleukin-4 (IL-4), interleukin-10 (IL-10), chemokine ligand 1 (CXCL1) and tumor necrosis factor-α (TNF-α) were determined by electro-chemiluminescence assays. The expression of p38 mitogen-activated protein kinase (p38 MAPK), phosphatidylinositol 3-kinase (PI3K), ribosome-associated complex-alpha serine/threonine-protein kinase (Akt), nuclear factor-κ-gene binding p65 (NF-κB p65), inhibitor of NF-κB-α (IκBα), glycogen synthase kinase-3β (GSK-3β), and their phosphorylated forms were tested by Western blot.</p><p><strong>Results: </strong>In GL, a total of 48 compounds were identified, including 14 flavonoids, 14 alkaloids, 11 carboxylic acids, 4 lactones, 2 glycosides, 2 terpenoids and 1 aldehyde. Network pharmacological analysis suggested that the mechanism of GL may be related to PI3K-Akt signaling pathway and cytokine expression. After treatment with GL, inflammatory pathological changes in the prostate of rats were significantly improved, and blood perfusion of the prostate was significantly decreased. GL reduced the expression of IFN-γ, CXCL1, TNF-α, IL-1β, iNOS, ICAM-1, p38 MAPK, p-p38 MAPK, PI3K, p-PI3K, NF-κB, p-NF-κB, IκBα, p-IκBα, GSK-3β, p-GSK-3β, p-Akt in CP rats, and increased the expression of IL-4 and IL-10 in CP rats.</p><p><strong>Conclusion: </strong>The chemical compositions of GL were first identified. GL can improve pathological changes in the prostate and recover the prostate blood perfusion of CP rats. The possible mechanisms of GL on CP involve increasing the expression of anti-inflammatory cytokines IL-4 and IL-10, inhibiting pro-inflammatory cytokines TNF-α, IL-1β, and IFN-γ, and down regulating the expression of CXCL1, iNOS, and ICAM-1 <i>via</i> inhibiting PI3K-Akt and NF-κB signaling pathway.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"89-99"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Promising effects of Persian shallot extract on the serum markers and blood pressure of patients with metabolic syndrome: a double-blinded randomized controlled trial.","authors":"Neda Naimipoor, Zahra Bagheri-Hosseinabadi, Mohammad Reza Hajizadeh, Mitra Abbasifard, Mohammad Reza Mirzaei, Akram Ghadiri Anari, Maryam Mohammad-Sadeghipour, Mehdi Mahmoodi","doi":"10.19852/j.cnki.jtcm.2025.01.008","DOIUrl":"10.19852/j.cnki.jtcm.2025.01.008","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the effect of Persian shallot extract on the serum markers and blood pressure of patients with metabolic syndrome (MetS).</p><p><strong>Methods: </strong>Fifty patients with MetS diagnosis were randomly assigned to the intervention (Persian shallot extract) and the control (placebo) group. Both groups received treatment for three months. Before the study and at the end of the study, 5 mL peripheral blood was taken from each patient. The measured factors included total antioxidant capacity (TAC), superoxide dismutase enzyme (SOD), malondialdehyde, oxidized low-density lipoprotein (Ox-LDL), apolipoprotein H (Apo-H), fasting blood glucose (FBS), total cholesterol, triglycerides, high-density lipoprotein (HDL), LDL, and systolic and diastolic blood pressure.</p><p><strong>Results: </strong>At baseline, the evaluated parameters were not significantly different between the intervention and control groups. At the end of the study, the mean serum levels of malondialdehyde and ox-LDL were significantly lower in the intervention group. The mean FBS, cholesterol, triglycerides, and LDL were significantly lower in the intervention group. The mean TAC and HDL were significantly higher in the intervention group (<i>P</i> < 0.05). Moreover, the intervention group significantly reduced systolic and diastolic blood pressure. No other significant association was observed.</p><p><strong>Conclusion: </strong>Persian shallot extract has several beneficial effects in MetS patients, including optimizing oxidative balance, reducing blood pressure, fasting blood sugar, and blood lipid profile.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"100-106"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764931/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of electroacupuncture on pain after periodontal flap surgery: a randomized controlled trial.","authors":"Emre Bulut, Didem Zkal Emino Lu, Yasemin Ay R","doi":"10.19852/j.cnki.jtcm.2025.01.018","DOIUrl":"10.19852/j.cnki.jtcm.2025.01.018","url":null,"abstract":"<p><strong>Objective: </strong>To examine the effect of electro-acupuncture (EA) application on postoperative pain levels and the amount of analgesic use in patients who underwent periodontal flap surgery.</p><p><strong>Methods: </strong>In this prospective, randomized and controlled study, patients planned for periodontal surgery were divided into two groups [EA group (<i>n =</i> 22): patients who received electroacupuncture and control group (<i>n =</i> 22): patients who did not receive electroacupuncture]. Electroacupuncture was applied bilaterally to the Hegu (LI4), Daying (ST5), and Jiache (ST5) points of the patients in the EA group immediately before and after the flap surgery for 30 min at a frequency of 50 Hz to each point at a current that the patient could tolerate. The patients in the control group underwent flap surgery without applying electroacupuncture. Patients were asked to record their pain levels on the visual analog scale and the number of analgesic tablets they took during the 7 postoperative days on the given form.</p><p><strong>Results: </strong>The total mean score of pain felt in the EA group (16.60 ± 2.78) was found to be significantly lower than that in the control group (31.37 ± 2.78) (<i>P =</i> 0.001). No significant difference was found between the groups regarding the amount of analgesics taken (4.77 ± 4.72, 5.82 ± 3.11) (<i>P =</i> 0.111).</p><p><strong>Conclusions: </strong>Our study shows that electro-acupuncture application is beneficial in reducing pain after periodontal flap surgery. Overall, more research is needed to fully understand the effects of electro-acupuncture on postoperative pain.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"184-191"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764923/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of combining Qingyanyin formulated granules with press needles in treating abdominal obesity: a multicenter randomized controlled trial.","authors":"Y E Wujie, Yang Yawei, Zhang Da, Tang Ling, Cui Minying, F U Bin, Zhang Meng, H U Xingang, Zhao Yan","doi":"10.19852/j.cnki.jtcm.2025.01.009","DOIUrl":"10.19852/j.cnki.jtcm.2025.01.009","url":null,"abstract":"<p><strong>Objective: </strong>To assess the efficacy and safety of the Qingyanyin formulated granules (, QYY), press needles (PN), and their combined application in addressing abdominal obesity (AO). This trial aims to offer a more scientifically grounded therapeutic regimen for clinical interventions.</p><p><strong>Methods: </strong>From March 2021 to July 2021, a multicenter, triple <i>－</i>blind, randomized 2 × 2 factorial design clinical trial was conducted across 7 centers in 4 major cities within mainland China. The trial participants were patients diagnosed with AO. The trial followed a 1∶1∶1∶1 random allocation ratio, assigning participants to one of four groups: QYY placebo plus simulated press needles (SPN) (placebo + SPN), QYY plus SPN (QYY + SPN), QYY placebo plus PN (placebo + PN), and QYY plus PN (QYY + PN). The trial participants received treatment for 12 weeks. Observe the changes in waist circumference, body weight, body mass index (BMI), Beck Depression Inventory (BDI), Beck Anxiety Inventory (BAI) scores, and Pittsburgh Sleep Quality Index (PSQI) before and after treatment.</p><p><strong>Results: </strong>The QYY + PN group exhibited significant improvements in waist circumference compared to placebo + PN [Difference = －1.59, 95% <i>CI</i> (－3.03, －0.16)] and placebo + SPN groups [Difference = －2.01, 95% <i>CI</i> (－3.46, －0.57)]. QYY + PN demonstrated a significant advantage over placebo + SPN [Difference = －2.01, 95% <i>CI</i> (－3.46, －0.57)], and no statistically significant interaction was observed between the two interventions (<i>P ></i> 0.05). In terms of weight and BMI improvements, the QYY + PN, QYY + SPN, and the PN + placebo groups all experienced trending greater reductions in weight compared to the placebo group. In terms of the total scores of PSQI, BAI, and BDI, all four groups exhibited improvements compared to the baseline. Specifically, concerning the change in total PSQI scores, the QYY + PN group exhibited a greater reduction; Regarding the change in total BAI scores, the PN + placebo group demonstrated a greater decrease;As for the change in total BDI scores, the QYY + SPN group displayed a greater reduction.</p><p><strong>Conclusion: </strong>This study confirmed that QYY + PN can effectively reduce the waist circumference of patients with AO. Furthermore, the combined approach offers greater benefits than either treatment alone, all without any reported serious adverse events.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"107-114"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbial characteristics of the damp-heat constitution: a population-based multicenter cross-sectional study.","authors":"L I Tianxing, Zhu Linghui, Wang Xueke, Tang Jun, Yang Lingling, Pang Guoming, L I Huang, Wang Liying, Dong Yang, Zhao Shipeng, L I Yingshuai, L I Lingru","doi":"10.19852/j.cnki.jtcm.2025.01.013","DOIUrl":"10.19852/j.cnki.jtcm.2025.01.013","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the difference in gut microbiota between population with damp-heat constitution (DHC) and balanced constitution (BC).</p><p><strong>Methods: </strong>A multi-centered cross-sectional case-control study was conducted, which included 249 participants with damp-heat constitution or balanced constitution. Baseline information of participants was collected, and stool samples were collected for gut microbiota analysis. Principal coordinate analysis, linear discriminant analysis effect size analysis, receiver operating characteristic, random forest model, and phylogenetic investigation of communities by reconstruction of unobserved states methods were used to reveal the relationship between gut microbiota and the damp-heat constitution.</p><p><strong>Results: </strong>Compared to those in the BC group, the richness and diversity of the microbiota, specifically those of several short-chain fatty acid producing genera such as Barnesiella, Coprobacter, and Butyricimonas, were significantly decreased in the DHC group. Regarding biological functions, flavonoid biosynthesis, propanoate metabolism, and nucleotide sugar metabolism were suppressed, while arachidonic acid metabolism and glutathione metabolism were enriched in the DHC group. Finally, a classifier based on the microbiota was constructed to discriminate between the DHC and BC populations.</p><p><strong>Conclusion: </strong>The gut microbiota of the DHC population exhibits significantly reduced diversity and is closely related to inflammation, metabolic disorders, and liver steatosis, which is consistent with clinical observations, thus serving as a potential diagnostic tool for traditional Chinese medicine constitution discrimination.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"140-151"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764936/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antidepressive and anxiolytic effects of a combination of Saffron and Chamomile in rats and their relationship with serotonin using methods.","authors":"Faiq Amin, Saara Ahmad, Muhammad Wasim, Asra Khan, Fazal Manzoor Arain, Zehra Batool, Saiqa Tabassum, Saima Khaliq, Noreen Samad, Saida Haider","doi":"10.19852/j.cnki.jtcm.2025.01.005","DOIUrl":"10.19852/j.cnki.jtcm.2025.01.005","url":null,"abstract":"<p><strong>Objective: </strong>To explore the potential of combining natural herbs like chamomile and saffron for the management of anxiety and depression.</p><p><strong>Methods: </strong>A rodent model of Major Depressive Disorder (MDD) and anxiety, secondary to streptozotocin-induced diabetes mellitus was made. A total of 6 rat groups were chosen; healthy and diseased controls; and diseased test groups of fluoxetine, saffron, chamomile, and combined saffron and chamomile treated (<i>n =</i> 6/group). Activity by forced swim test (FST), elevated plus maze test (EPMT), and correlations with biochemical markers like serum glucose, tryptophan, C-reactive protein (CRP), brain derived neurotrophic factor (BDNF) and 5-hydrox-ytryptamine 2C receptor (5HT2CR) expression, were assessed at the end of the 3rd week of the treatment. A one-way analysis of variance with a post-hoc Tukey's test was applied.</p><p><strong>Results: </strong>The combined herbal treatment group showed significantly better (<i>P <</i>0.05) than all other groups in terms of anti-hyperglycemic effect. All treatments improved the CRP levels; however, the combination group was also significantly better than fluoxetine and the individual herb groups. Only the herb groups showed efficacy in the FST with added benefits of the combination group over the healthy controls and similar trends in the EPMT. However, expression of 5HT2CR was repressed while BDNF was elevated through treatment.</p><p><strong>Conclusion: </strong>This study shows that in comparison to treatment with a SSRI, and individual herbs, the combination of chamomile and saffron showed overall improved outcomes.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"49-56"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Network pharmacology-based study on the mechanism of Tangfukang formula against type 2 diabetes mellitus.","authors":"Yan Kai, Wang Wei, Wang Yan, Gao Huijuan, Feng Xingzhong","doi":"10.19852/j.cnki.jtcm.2025.01.007","DOIUrl":"10.19852/j.cnki.jtcm.2025.01.007","url":null,"abstract":"<p><strong>Objective: </strong>To explore the mechanism of Tangfukang formula (, TFK) in treating type 2 diabetes mellitus (T2DM).</p><p><strong>Methods: </strong>We employed network pharmacology combined with experimental validation to explore the potential mechanism of TFK against T2DM. Initially, we filtered bioactive compounds with the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) and Symptom Mapping (SymMap), and gathered targets of TFK and T2DM. Subsequently, we constructed a protein-protein interaction (PPI) network, enriched core targets through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), and adopted molecular docking to study the binding mode of compounds and the signaling pathway. Finally, we employed a KKAy mice model to investigate the effect and mechanism of TFK against T2DM. Biochemical assay, histology assay, and Western blot (WB) were used to assess the mechanism.</p><p><strong>Results: </strong>There were 492 bioactive compounds of TFK screened, and 1226 overlapping targets of TFK against T2DM identified. A compound-T2DM-related target network with 997 nodes and 4439 edges was constructed. KEGG enrichment analysis identified some core pathways related to T2DM, including adenosine 5-monophosphate-activated protein kinase (AMPK) signaling pathway. Molecular docking study revealed that compounds of TFK, including citric acid, could bind to the active pocket of AMPK crystal structure with free binding energy of －4.8, －8 and －7.9, respectively. Animal experiments indicated that TFK decreased body weight, fasting blood glucose, fasting serum insulin, homeostasis model of insulin resistance, glycosylated serum protein, total cholesterol, triglyceride, and low-density lipoprotein cholesterol, and improve oral glucose tolerance test results. TFK reduced steatosis in liver tissue, and infiltration of inflammatory cells, and protected liver cells to a certain extent. WB analysis revealed that, TFK upregulated the phosphorylation of AMPK and branched-chain α-ketoacid dehydrogenase proteins.</p><p><strong>Conclusion: </strong>TFK has the potential to effectively manage T2DM, possibly by regulating the AMPK signaling pathway. The present study lays a new foundation for the therapeutic application of TFK in the treatment of T2DM.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"76-88"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764938/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}