Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan最新文献

{"title":"Research on acupuncture robots based on the OptiTrack motion capture system and a robotic arm.","authors":"H E Ling, Yang Hui, L I Kang, Wang Junwen, Sun Zhibo, Yang Jinsheng, Zhang Jing","doi":"10.19852/j.cnki.jtcm.2025.01.020","DOIUrl":"10.19852/j.cnki.jtcm.2025.01.020","url":null,"abstract":"<p><strong>Objective: </strong>To propose an automatic acupuncture robot system for performing acupuncture operations.</p><p><strong>Methods: </strong>The acupuncture robot system consists of three components: automatic acupoint localization, acupuncture manipulations, and <i>De Qi</i> sensation detection. The OptiTrack motion capture system is used to locate acupoints, which are then translated into coordinates in the robot control system. A flexible collaborative robot with an intelligent gripper is then used to perform acupuncture manipulations with high precision. In addition, a <i>De Qi</i> sensation detection system is proposed to evaluate the effect of acupuncture. To verify the stability of the designed acupuncture robot, acupoints' coordinates localized by the acupuncture robot are compared with the Gold Standard labeled by a professional acupuncturist using significant level tests.</p><p><strong>Results: </strong>Through repeated experiments for eight acupoints, the acupuncture robot achieved a positioning error within 3.3 mm, which is within the allowable range of needle extraction and acupoint insertion. During needle insertion, the robot arm followed the prescribed trajectory with a mean deviation distance of 0.02 mm and a deviation angle of less than 0.15°. The results of the lifting thrusting operation in the Xingzhen process show that the mean acupuncture depth error of the designed acupuncture robot is approximately 2 mm, which is within the recommended depth range for the Xingzhen operation. In addition, the average detection accuracy of the <i>De Qi</i> keywords is 94.52%, which meets the requirements of acupuncture effect testing for different dialects.</p><p><strong>Conclusion: </strong>The proposed acupuncture robot system streamlines the acupuncture process, increases efficiency, and reduces practitioner fatigue, while also allowing for the quantification of acupuncture manipulations and evaluation of therapeutic effects. The development of an acupuncture robot system has the potential to revolutionize low back pain treatment and improve patient outcomes.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"201-212"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atractylenolide I ameliorates post-infectious irritable bowel syndrome by inhibiting the polymerase I and transcript release factor and c-Jun N-terminal kinase/inducible nitric oxide synthase pathway.","authors":"Yuan Jianan, Cheng Kunming, L I Chao, Zhang Xiang, Ding Zeyu, L I Bing, Zheng Yongqiu","doi":"10.19852/j.cnki.jtcm.2025.01.006","DOIUrl":"10.19852/j.cnki.jtcm.2025.01.006","url":null,"abstract":"<p><strong>Objective: </strong>To explore the therapeutic effect and target of atractylenolide I (AT-I) on post-infectious irritable bowel syndrome (PI-IBS) rats.</p><p><strong>Methods: </strong>Therefore, the preliminarily mechanism of AT-I in anti-PI-IBS were first predicted by network pharmacology and molecular docking, then the possible signaling pathways were systematically analyzed. Finally, the potential therapeutic targets and possible signaling pathways of AT-I on PI-IBS in Sprague-Dawley (SD) rat model were verified by experiments.</p><p><strong>Results: </strong>AT-I could alleviate PI-IBS symptoms and reduce the expression of tumor necrosis factor α, interleukin-6 and Interferon-gamma in PI-IBS SD rat model and inhibit the c-Jun N-terminal kinase/inducible nitric oxide synthase (JNK/iNOS) pathway. Notably, AT-I treatment could inhibit the overexpression of polymerase I and transcript release factor (PTRF).</p><p><strong>Conclusion: </strong>AT-I could alleviate PI-IBS symptoms through downregulation of PTRF and inhibiting the JNK/ iNOS pathway. This study not only provides a scientific basis to clarify the anti-PI-IBS effect of AT-I and its mechanism but also suggests a novel promising therapeutic strategy to treat the PI-IBS.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"57-65"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764933/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating machine learning and human use experience to identify personalized pharmacotherapy in Traditional Chinese Medicine: a case study on resistant hypertension.","authors":"Che Qianzi, Liu Dasheng, Xiang Xinghua, Tian Yaxin, Xie Feibiao, X U Wenyuan, Liu Jian, Wang Xuejie, Wang Liying, Bai Weiguo, Han Xuejie, Yang Wei","doi":"10.19852/j.cnki.jtcm.2025.01.019","DOIUrl":"10.19852/j.cnki.jtcm.2025.01.019","url":null,"abstract":"<p><strong>Objective: </strong>To enhance the understanding of identifying personalized pharmacotherapy options in Traditional Chinese Medicine (TCM), and further support the registration of new TCM drugs.</p><p><strong>Methods: </strong>Generalized Boosted Models and XGBoost were employed to construct a classification model to identify the bad prognosis factors in resistant hypertension (RH) patients. Furthermore, we used association analysis to explore the rules of \"symptom-syndrome\" and \"symptom-herb\" for the major influencing factors, in order to summarize prescription pattern and applicable patients of TCM.</p><p><strong>Results: </strong>Patients with major adverse cardiac events mostly have complex symptoms of phlegm, stasis, deficiency and fire intermingled with each other, and finally summarized the human experience of using Chinese herbal medicine to precisely intervene in some symptoms of RH patients on the basis of conventional Western medical treatment.</p><p><strong>Conclusions: </strong>Machine learning algorithms can make full use of human use experience and evidence to save clinical trial resources and accelerate the development of TCM varieties.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"192-200"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764932/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hewei Jiangni granule alleviates visceral hypersensitivity of non-erosive reflux diseasestromal interaction molecule 1/transient receptor potential vanilloid subfamily member 1 pathway.","authors":"Cheng Yuan, Zhang Xiaosi, L I Junxiang, Zhang Liming, Dai Yi, Xie Chune, Shi Lei, L I Xiaohong, Kou Fushun","doi":"10.19852/j.cnki.jtcm.2025.01.001","DOIUrl":"10.19852/j.cnki.jtcm.2025.01.001","url":null,"abstract":"<p><strong>Objective: </strong>To explore if Hewei Jiangni granule (, HWJNG) could regulate esophageal hypersensitivity via stromal interaction molecule 1 (STIM1)/transient receptor potential vanilloid subfamily member 1 (TRPV1) pathway.</p><p><strong>Methods: </strong>Qualitative analysis of HWJNG was analysis by high performance of liquid and gas chromatography. <i>In vivo</i>, animal model of non-erosive reflux disease (NERD) was established by fructose intake and restraint stress. HWJNG and Omeprazole were administered by gavage to the drug intervention group. Reflux and visceral hypersensitivity were analyzed by pathological changes, PH value test, mechanical paw withdrawal threshold, thermal withdrawal latency and mast cells (MCs) degranulation. <i>In vitro</i>, substance P (SP)-induced P815 cells and dorsal root ganglion (DRG) cells were co-cultured. Expression in both mice and cells of STIM1, TRPV1, and esophageal visceral hypersensitivity-related gastrointestinal neurochemicals were validated by enzyme linked immunosorbent assays, quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. Moreover, overexpression and small interfering RNA against STIM1 were utilized to verify of the role of HWJNG in DRG cells.</p><p><strong>Results: </strong>HWJNG significantly suppressed intercellular space widening, injury of mitochondrial, MCs degranulation, mechanical allodynia and heat neuropathic sensory and increased pH value of esophageal mucosa in NERD mice. HWJNG inhibited expression of visceral hypersensitivity-related gastrointestinal neurochemicals in esophageal mucosa and activated P815 cells, and expression of the STIM1, TRPV1 and related neurotransmitters in DRG and DRG cells. STIM1 siRNA and HWJNG both reduced P815 cells adhesion to DRGs cells and Ca²⁺ flow into the cytoplasmic space of DRG cells. Furthermore, HWJNG could reversed STIM1 overexpression induced upregulation of TRPV1.</p><p><strong>Conclusion: </strong>HWJNG suppressed intercellular space widening in NERD mice, stabilized MCs and restored neuronal hyperexcitability by regulating visceral hypersensitivity <i>via</i>STIM1/TRPV1 pathway.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764939/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the diagnostic criteria of syndrome of dampness obstruction in idiopathic membranous nephropathy based on expert consensus.","authors":"Wang Dan, Huang Xiaoyan, L I Ping, Hong Xiaofan, Zhang Yumin, Wang Rongrong, Bao Kun, Liang Xing","doi":"10.19852/j.cnki.jtcm.2025.01.016","DOIUrl":"10.19852/j.cnki.jtcm.2025.01.016","url":null,"abstract":"<p><strong>Objective: </strong>To reach consensus on the diagnostic criteria of syndrome of dampness obstruction in idiopathic membranous nephropathy (IMN) patients by literature research and expert investigation (interviews and a Delphi method).</p><p><strong>Methods: </strong>Our study was consistent with T/CACM 1336-2020. We searched the monographs and references published in the past 40 years (1983-2022), and established the diagnostic criteria pool of water-dampness syndrome and dampness-turbidity syndrome in Traditional Chinese Medicine (TCM) based on literature by using frequency statistics and correlation analysis. Expert investigation (interview method and two rounds of Delphi method) was used to form the diagnostic criteria of water-dampness syndrome and dampness-turbidity syndrome of idiopathic membranous nephropathy. Clinical diagnostic test research was carried out, and compared with \"Diagnostic Criteria for dampness syndrome\" (T/CACM 1454-2023) to evaluate the authenticity, reliability and clinical application value of the standard.</p><p><strong>Results: </strong>A total of 122 relevant guides, standards, monographs and documents were included through searching books and Chinese databases. Four experts were interviewed and two rounds of delphi method (75 experts nationwide) were carried out. The experts' opinions are relatively concentrated and the differences are small. Based on the weight of each index, the diagnostic criteria indexes of water-dampness syndrome and dampness-turbidity syndrome were selected. After discussion by the core group members, the diagnostic model of \"necessary symptoms and optional symptoms\" was established, and the final diagnostic criteria of water-dampness syndrome and dampness-turbidity syndrome were established. One hundred and ninety-one inpatients and outpatients of Guangdong Provincial Hospital of Chinese Medicine from January 2021 to February 2023 were included in Diagnostic test study. There was no statistical difference in gender, age and course of disease (<i>P ></i> 0.05). The sensitivity and specificity of the trial standard were 90.34% and 73.33% respectively, while the sensitivity and specificity of T/CACM 1454-2023 were 99.43% and 6.67%, respectively.</p><p><strong>Conclusions: </strong>The consensus-based diagnostic criteria for IMN can be widely incorporated in TCM. A further clinical study will be conducted to analyze the diagnosis value and cut-off score of our IMN criteria.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"167-175"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764929/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening optimal target populations with symptomatic bradyarrhythmia for pharmacotherapy: a discriminant analysis pilot study.","authors":"Zhang Wantong, Y I Danhui, L U Fang, Yang Qiaoning, Shi Shuai, L I Qiuyan, Weng Weiliang, Wang Xujie, Zhu Baochen","doi":"10.19852/j.cnki.jtcm.2025.01.015","DOIUrl":"10.19852/j.cnki.jtcm.2025.01.015","url":null,"abstract":"<p><strong>Objective: </strong>To screen the target population with symptomatic bradyarrhythmia for pharmacotherapy.</p><p><strong>Methods: </strong>This study use database from clinical trial that use Yuanjiang decoction, a traditional Chinese medicinal prescription. Eligible participants were recruited and treated with Yuanjiang decoction (composed of 6 Chinese herbal medicines), 200 mL twice daily. Cross-contingency analysis, logistic regression analysis, interaction analysis, discriminant analysis and 10-fold cross validation methods were used to establish discriminant model.</p><p><strong>Results: </strong>This study concluded that the clinical treatment of bradyarrhythmia has a clear effect. Low minimum heart rate, high maximum heart rate are risk factors that affect the efficacy. Patients with only one comorbid disease did not significantly affect the efficacy, but patient with two or more diseases of coronary heart disease, hypertension, paroxysmal atrial fibrillation, premature ventricular contraction and premature atrial contraction at the same time did not have a good effect. Using the discriminant analysis method to establish a efficacy prediction model, y = 0.07 X 1 + 0.16 X 2 - 0.65 X 3 - 1.12 X 4 - 0.71 X 5 - 0.75 X 6 - 0.91 X 7 (X1 = 24 h mean heart rate, X2 = minimum heart rate, X3= coronary heart disease, X4 = paroxysmal atrial fibrillation, X5 = premature ventricular contraction, X6= sinus block, X7 = atrioventricular block).</p><p><strong>Conclusion: </strong>Our model based on the clinical features of patients with bradyarrhythmia. Should be useful aid for predicting pharmacotherapy response and could screen the optimal pharmacotherapy target.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"160-166"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764922/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of add-on therapy with Traditional Chinese Medicine on the survival of patients with anemic lower-risk myelodysplastic syndromes in the real-world setting: a retrospective study.","authors":"Q U Weiying, Tan Xyucheng, Zhao Yihan, Y U Yanan, Zhao Lin","doi":"10.19852/j.cnki.jtcm.2025.01.014","DOIUrl":"10.19852/j.cnki.jtcm.2025.01.014","url":null,"abstract":"<p><strong>Objective: </strong>To illustrate the effect of add-on therapy of Traditional Chinese Medicine (TCM) on the long-term survival of anemic lower-risk myelodysplastic syndromes (LR-MDS) patients.</p><p><strong>Methods: </strong>In this study, we conducted a retrospective analysis of the data of patients with LR-MDS who were identified from Shuguang Hospital between January 2006 and June 2020. The Kaplan-Meier method was used to calculate overall survival (OS), while the Cox model was employed to analyze prognostic factors of long-term survival. A total of 162 patients who met the inclusion criteria were divided into two groups: the TCM group (<i>n =</i> 78) or the non-TCM group (<i>n =</i> 84).</p><p><strong>Results: </strong>There were no significant differences in baseline characteristics. The cumulative survival rate of the TCM group was significantly higher than that of the non-TCM group [log-rank <i>P =</i> 0.029, hazard ratio (<i>HR</i>) = 0.484, 95% confidence interval (<i>CI</i>) (0.249, 0.942), <i>P <</i> 0.05]. Patients with longer TCM treatment appeared to have better OS (≥ 12 months <i>vs</i> < 12 months); [<i>HR</i>, 0.166 95% <i>CI</i> (0.055, 0.508), <i>P <</i> 0.001)]. According to the Cox model analysis, the combination of TCM and conventional Western Medicine may be a protective factor affecting the long-term survival of LR-MDS patients [<i>HR</i> = 0.509, 95% <i>CI</i>, (0.261, 0.993), <i>P =</i> 0.048]. The hematologic improvement erythroid response (HI-E) rate of the TCM group was significantly higher than that of the non-TCM group (69.23% <i>vs</i> 52.38%; <i>P =</i> 0.028). However, the duration of HI-E and cases of disease progression after HI-E did not significantly differ from the non-TCM group (<i>P ></i> 0.05).</p><p><strong>Conclusions: </strong>The integration of TCM and Western Medicine has the potential to extend the OS of LR-MDS patients when compared to the use of Western Medicine alone.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"152-159"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764935/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative proteomic analysis of the brain reveals the potential antidepressant mechanism of Jiawei Danzhi Xiaoyao San in a chronic unpredictable mild stress mouse model of depression.","authors":"L I Yajing, Wang Baoying, Shao Wenxue, L U Shuaifei, S U Pan, Bai Ming, X U Erping, L I Yucheng","doi":"10.19852/j.cnki.jtcm.2025.01.003","DOIUrl":"10.19852/j.cnki.jtcm.2025.01.003","url":null,"abstract":"<p><strong>Objective: </strong>To reveal the antidepressant mechanisms of Jiawei DanZhiXiaoYaoSan (,JD) in chronic unpredictable mild stress (CUMS)-induced depression in mice.</p><p><strong>Methods: </strong>Using the CUMS mouse model of depression, the antidepressant effects of JD were assessed using the sucrose preference test (SPT), forced swimming test (FST), and tail suspension test (TST). Tandem mass tag (TMT)-based quantitative proteomic analysis of the brain was performed following JD treatment. Hierarchical clustering, Gene Ontology function annotation, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, and protein-protein interactions (PPIs) were used to analyze differentially expressed proteins (DEPs), which were further validated using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting.</p><p><strong>Results: </strong>Behavioral tests confirmed the anti-depressant effects of JD, and bioinformatics analysis revealed 59 DEPs, including 33 up-regulated and 26 down-regulated proteins, between the CUMS and JD-M groups. KEGG and PPI analyses revealed that neuro-filament proteins and the Ras signaling pathway may be key targets of JD in the treatment of depression. qRT-PCR and Western blotting results demonstrated that CUMS reduced the protein expression of neurofilament light (NEFL) and medium (NEFM) and inhibited the phosphorylation of extracellular regulated kinase 1/2 (ERK1/2), whereas JD promoted the phosphorylation of ERK1/2 and up-regulated the protein expression of NEFL and NEFM.</p><p><strong>Conclusions: </strong>The antidepressant mechanism of JD may be related to the up-regulation of p-ERK1/2 and neurofilament proteins.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 1","pages":"22-31"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chinese patent medicine for atherosclerosis: a systematic review and Meta-analysis of randomized controlled trials.","authors":"X U Jian, Liu Yuntao, Luo Zhihao, Zhao Zhen, Wang Dawei, Liu Qing","doi":"10.19852/j.cnki.jtcm.2024.06.001","DOIUrl":"10.19852/j.cnki.jtcm.2024.06.001","url":null,"abstract":"<p><strong>Objective: </strong>To synthesize the evidence from randomized controlled trials (RCTs) to assess the efficacy and safety of Chinese patent medicine (CPM) on atherosclerosis (AS) or with a high risk of atherosclerosis.</p><p><strong>Methods: </strong>All RCTs in three databases (PubMed, EMBASE, and Cochrane Library) were included from the inception of the database to September 20, 2019. The methodological evaluation of the included trials was carried out using the Cochrane Collaboration Risk of Bias Tool. Meta-analysis was conducted using RevMan 5.3 software. The Grading of Recommendations Assessment, Development and Evaluation (GRADE) methodology was used to evaluate the quality of evidence.</p><p><strong>Results: </strong>Eighteen RCTs were included, involving a total of 3885 patients with AS or with a high risk of AS. Most trials had favorable methodology. Meta-analysis suggested significant differences in clinical endpoint (<i>n =</i> 1938, <i>RR</i> 0.53; 95% <i>CI</i> 0.40 to 0.69, <i>P <</i> 0.00001; <i>I</i> ² = 0%); the change in carotid artery IMT (<i>n =</i> 1723, <i>MD</i> -0.09, 95% <i>CI</i> －0.14 to -0.04, <i>P <</i> 0.001; <i>I</i> ² = 40%); change in FMD (<i>n =</i> 239, <i>MD</i> 0.87, 95% <i>CI</i> 0.52 to 1.21, <i>P <</i> 0.00001; <i>I</i> ² = 0%); change in high sensitive C-reactive protein (hs-CRP) (<i>n =</i> 1527, <i>MD</i> －1.89, 95% <i>CI</i> －3.36 to －0.42, <i>P =</i> 0.01; <i>I</i> ² = 94%) and incidence of total adverse events (<i>RR</i> 0.76, 95% <i>CI</i> 0.62 to 0.93, <i>P =</i> 0.009; <i>I</i> ² = 40%) in favor of the experimental group. However, meta-analysis showed no significant differences in the change in low-density lipoprotein-C (LDL-C) (<i>n =</i> 2419, <i>MD</i> －0.19, 95% <i>CI</i> －0.50 to 0.12, <i>P =</i> 0.22, <i>I</i> ² = 94%) between the experimental and control groups.</p><p><strong>Conclusion: </strong>CPM could have certain clinical efficacy in the treatment of AS. However, more double-blinded placebo-controlled RCTs are required in further evaluations to provide stronger evidence.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"44 6","pages":"1082-1090"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11589558/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142775900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome sequencing-based study on the mechanism of action of Jintiange capsules in regulating synovial mesenchymal stem cells exosomal miRNA and articular chondrocytes mRNA for the treatment of osteoarthritis.","authors":"Chen Zhongying, Zhang Xue, Zhang Xiaofei, Zou Junbo, Yuan Puwei, Shi Yajun","doi":"10.19852/j.cnki.jtcm.20240927.004","DOIUrl":"10.19852/j.cnki.jtcm.20240927.004","url":null,"abstract":"<p><strong>Objective: </strong>To corroborate the efficacy of Jintiange capsules (JTGs) in the treatment of osteoarthritis (OA) by exploring the potential mechanism of action of synovial mesenchymal stem cell exosomes (SMSC-Exos) and articular chondrocytes (ACs) through transcriptome sequencing (RNA-seq).</p><p><strong>Methods: </strong>Type II collagenase was used to induce OA in rats. The efficacy of JTGs was confirmed by macroscopic observation of articular cartilage, micro-CT observation, and safranin fast green staining. After SMSC-Exos and ACs were qualified, RNA-seq was used to screen differentially expressed miRNAs and mRNAs. The target genes of differentially expressed miRNAs in Synovial mesenchymal stem cells (SMSCs) were predicted based on the multiMiR R package. The co-differentially expressed genes of SMSC-Exos and ACs were obtained by venny 2.1.0. The miRNA-mRNA regulatory network was constructed by Cytoscape software. Based on the OmicShare platform, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis was performed on the mRNA regulated by key miRNAs. Expression trend analysis was performed for co-differentially expressed genes. Correlation analysis was performed on micro-CT efficacy indicators, co-differentially expressed genes mRNA and miRNA.</p><p><strong>Results: </strong>The efficacy of each administration group of JTGs was significant compared with the model group. SMSC-Exos and ACs were identified by their characteristics. The expression of rno-miR-23a-3p, rno-miR-342-3p, rno-miR-146b-5p, rno-miR-501-3p, rno-miR-214-3p was down-regulated in OA pathological state, and the expression of rno-miR-222-3p, rno-miR-30e-3p, rno-miR-676, and rno-miR-192-5p expression was up-regulated, and the expression of all these miRNAs was reversed after the intervention with JTGs containing serum. The co-differentially expressed genes were enriched in the interleukin 17 signaling pathway, tumor necrosis factor signaling pathway, transforming growth factor-β signaling pathway, etc. The expression trends of Ccl7, Akap12, Grem2, Egln3, Arhgdib, Ccl20, Mmp12, Pla2g2a, and Nr4a1 were significant. There was a correlation between micro-CT pharmacodynamic index, mRNA, and miRNA.</p><p><strong>Conclusion: </strong>JTGs can improve the degeneration of joint cartilage and achieve the purpose of cartilage protection, which can be used for the treatment of OA. SMSCs-related miRNA expression profiles were significantly altered after the intervention with JTGs containing serum. The 9 co-differentially expressed genes may be the key targets for the efficacy of JTGs in the treatment of OA rats, which can be used for subsequent validation.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"44 6","pages":"1153-1167"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11589567/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142776026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}