Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan最新文献

{"title":"Mechanism of Yangxin Dawa Yimixike Mi Gao for myocardial ischemia/reperfusion injury: an integrative study of network pharmacology and and validation.","authors":"Dong Yuwei, Ran Qingzhi, Abdulla Rahima, Maitinuer Maiwulanjiang, Tian Fang, Aisa Haji Akber","doi":"10.19852/j.cnki.jtcm.2026.04.013","DOIUrl":"10.19852/j.cnki.jtcm.2026.04.013","url":null,"abstract":"<p><strong>Objective: </strong>To systematically elucidate the cardioprotective mechanisms of the traditional Uyghur polyherbal formulation Yangxin Dawa Yimixike Mi Gao (YXDW, ) against myocardial ischemia/ reperfusion injury through integrated chemical analysis, bioinformatics prediction, and multilevel experimental validation.</p><p><strong>Methods: </strong>Using ultra-high-performance liquid chromatography coupled with quadrupole-Orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap-HRMS), 137 bioactive constituents were identified in YXDW. Potential targets associated with myocardial ischemia/reperfusion (I/R) injury were predicted through network pharmacology analysis and validated using Mendelian randomization (MR). Gene ontology and kyoto encyclopedia of genes and genomes enrichment analyses, together with molecular docking, were performed to identify the core regulatory pathways. An <i>in vivo</i> myocardial I/R model and an <i>in vitro</i> hypoxia/ reoxygenation (H/R) model were established in Sprague-Dawley rats and H9c2 cardiomyocytes, respectively. The therapeutic effects of YXDW were evaluated using histopathological examination, biochemical markers, including creatine kinase (CK), lactate dehydrogenase (LDH), superoxide dismutase, C-reactive protein, interleukin-6, and tumor necrosis factor-alpha, echocardiography, enzyme-linked immunosorbent assay, western blotting, and quantitative reverse transcription polymerase chain reaction.</p><p><strong>Results: </strong>UHPLC-Q-Orbitrap-HRMS fingerprinting demonstrated high batch-to-batch consistency, with similarity values greater than 0.87, and identified 23 compounds with high oral bioavailability (OB ≥ 56%). Network pharmacology and MR analyses identified 13 key targets associated with myocardial I/R injury, including C-X-C motif chemokine receptor 1 (CXCR1), mitogen-activated protein kinase kinase 1 (MAP2K1), and poly (ADP-ribose) polymerase 1 (PARP1), which may be involved in the regulation of apoptosis, oxidative stress, and inflammatory responses. <i>In vivo</i> experiments showed that YXDW administration alleviated I/R-induced myocardial injury, as evidenced by reduced serum CK and LDH levels, attenuated myocardial apoptosis, and improved left ventricular function. Furthermore, the <i>in vivo</i> and <i>in vitro</i> results showed that YXDW dose-dependently downregulated CXCR1, MAP2K1, and PARP1 expression at both the protein and messenger RNA (mRNA) levels in myocardial tissues and H/R-injured H9c2 cardiomyocytes.</p><p><strong>Conclusion: </strong>YXDW exerts cardioprotective effects against myocardial I/R injury through multi-target modulation of inflammatory, oxidative, and apoptotic pathways. Our findings provide preliminary mechanistic support for the clinical relevance of YXDW in I/R therapy and offer a modern pharmacological basis for the rational use of traditional polyherbal formulations.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 4","pages":"927-940"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13494876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148815440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and safety of Dingluo nasal drops for the acute treatment of migraine: a pilot randomized trial.","authors":"Yao Yuning, A N Yuqiu, Liu Jiaojiao, D U Boxuan, Cao Kegang","doi":"10.19852/j.cnki.jtcm.2026.04.014","DOIUrl":"10.19852/j.cnki.jtcm.2026.04.014","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the efficacy and safety of two doses of Dingluo nasal drops compared to placebo in the acute treatment of migraine.</p><p><strong>Methods: </strong>This randomized, parallel-treatment, placebo-controlled, double-blind trial was conducted at Dongzhimen hospital, Beijing University of Chinese Medicine. Eligible participants were randomly assigned (1∶1∶1) to receive either full-dose Dingluo nasal drops, half-dose Dingluo nasal drops, or a placebo for three migraine attacks. The primary endpoint was the proportion of participants achieving pain freedom at 2 h postdose, assessed in all randomly assigned participants who received study medication.</p><p><strong>Results: </strong>A total of 91 participants with 257 attacks were included in the statistical analysis. At 2 h postdose, Dingluo nasal drops were superior to placebo for freedom from pain [high dose <i>vs</i>placebo: 14.61% <i>vs</i>1.20%, <i>P</i> = 0.001, risk difference 13.40, 95% <i>CI</i>(5.70, 21.11); low dose <i>vs</i>placebo: 17.65% <i>vs</i>1.20%, <i>P <</i>0.001, risk difference: 16.44, 95% CI (8.00, 24.88)]. The high dose and the low dose effected similarly for freedom from pain [14.61% <i>vs</i>17.65%, <i>P =</i> 0.585, risk difference: －3.04, 95% <i>CI</i>(－13.97, 7.89)]. It showed a good efficacy in pain relief. The most common adverse events were nasal burning and nasal itchiness. All adverse events are transient and dissipate within a relatively short time frame.</p><p><strong>Conclusions: </strong>Dingluo nasal drops demonstrated superior efficacy to placebo in achieving pain freedom for acute migraine treatment. Mild nasal reactions were observed, with a trend toward reduced discomfort at the half-dose concentration.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 4","pages":"941-950"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13494879/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148815358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroacupuncture alleviates intestinal inflammation in rats with ulcerative colitis by regulating colonic cell autophagy and gut microbiota.","authors":"X U Huichao, Hao Jianheng, Chang Boya, Wang Danni, L I Rangqian, Cao Yuxia, Wang Haijun, J I Laixi","doi":"10.19852/j.cnki.jtcm.2026.04.010","DOIUrl":"10.19852/j.cnki.jtcm.2026.04.010","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects of electroacupuncture (EA) on autophagy and gut microbiota homeostasis in a dextran sulfate sodium (DSS)-induced rat model of ulcerative colitis (UC).</p><p><strong>Methods: </strong>UC rats received EA treatment, and efficacy was evaluated by disease activity index (DAI), colon length, serum cytokines, and histopathology. Autophagy was assessed by transmission electron microscopy (TEM), reverse transcription quantitative polymerase chain reaction, and Western blotting for autophagy-related protein (ATG)5, ATG12, microtubule-associated protein 1 light chain 3 (LC3), and sequestosome 1 (p62), with phosphatidylinositol 3-kinase/protein kinase B/ mammalian target of rapamycin (PI3K/AKT/mTOR) pathway analysis. Gut microbiota composition was profiled by 16S ribosomal RNA (rRNA) gene sequencing, and functional relevance was confirmed by fecal microbiota transplantation (FMT).</p><p><strong>Results: </strong>EA reduced DAI scores, preserved colon length, lowered tumor necrosis factor-α and interleukin-1β (IL-1β), and elevated IL-2 and IL-10. Colonic injury and inflammatory infiltration were alleviated, autophagy markers improved, and PI3K/AKT/mTOR pathway activity was suppressed. EA also enhanced microbiota diversity and composition, with FMT confirming its microbiota-autophagy-inflammation modulatory role.</p><p><strong>Conclusion: </strong>EA alleviates UC by promoting colonic epithelial autophagy through PI3K/AKT/mTOR pathway inhibition and restoring gut microbiota balance, offering a promising therapeutic strategy.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 4","pages":"894-906"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13494884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148815456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Berberine alleviates alcoholic liver diseaseactivating intestinal nuclear receptor subfamily 1 group D member 1.","authors":"Huang Yuwei, Cai Yuting, L I Zanjin, W U Zicong, Guo Lianxia, Lin Luomin, Dong Linlin, W U Baojian, Dong Dong","doi":"10.19852/j.cnki.jtcm.2026.04.002","DOIUrl":"10.19852/j.cnki.jtcm.2026.04.002","url":null,"abstract":"<p><strong>Objectives: </strong>To elucidate the hepatoprotective mechanism of berberine (BBR) against alcohol-induced liver injury.</p><p><strong>Methods: </strong>Chronic and acute alcohol-induced liver injury models in mice were established to mimic human alcoholic liver disease (ALD). The therapeutic effect of BBR was evaluated in both models. The severity of liver injury, lipid metabolism and circadian clock related factors were assessed using histopathology, biochemical assays, and Western blotting. Intestinal-specific nuclear receptor subfamily 1 group D member 1 (<i>Rev-erbα</i>) knockout mice were used to validate intestinal <i>Rev-erbα</i> as the key mediator.</p><p><strong>Results: </strong>BBR alleviated hepatic steatosis and inflammation in both chronic and acute ALD models. Mechanistically, BBR activated intestinal REV-ERBα, upregulating intestinal fatty acid desaturase 2 (FADS2) expression, suppressing hepatic sterol regulatory element binding protein-1 (SREBP-1c) and its downstream lipogenic genes. These effects were abolished in intestinal specific Rev-erba knockout mice. BBR was most effective against ALD when administered during the peak expression phase of REV-ERBα (Zeitgeber Time 6).</p><p><strong>Conclusions: </strong>Intestinal REV-ERBα represents a promising therapeutic target for ALD, and chrono-modulated BBR administration may enhance treatment efficacy. This study underscores the role of gut-liver axis in ALD pathogenesis and provides a rationale for developing time-tailored therapies.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 4","pages":"788-797"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13494885/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148815376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taohong Huazhuo decoction alleviates carbon tetrachloride-induced hepatic fibrosis in rats by inhibiting the nicotinamide adenine dinucleotide phosphate oxidase 4/nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3 signaling pathway.","authors":"Ren Yuan, Chen Xiangni, Shi Lei, Mao Minghua, L I Shufang, Yao Jia, Hao Jianmei","doi":"10.19852/j.cnki.jtcm.2026.04.008","DOIUrl":"10.19852/j.cnki.jtcm.2026.04.008","url":null,"abstract":"<p><strong>Objective: </strong>To explore the mechanism underlying the anti-fibrotic effects of Taohong Huazhuo decoction (THHZD, ).</p><p><strong>Methods: </strong>Forty percent carbon tetrachloride (CCl₄) dissolved in olive oil (2 mL/kg) was intraperitoneally injected twice a week for 8 weeks to induce hepatic fibrosis in rats. The normal group received the same volume of olive oil alone. At week 5, rats injected with CCl4 were intragastrically administered normal saline, 0.1 mg/kg colchicine, 3.285 g/kg THHZD, 6.57 g/kg THHZD, and 13.14 g/kg THHZD once a day for 4 weeks. A biochemical analyzer was used to detect the levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and total bilirubin (TBIL). Hematoxylin and eosin (HE) staining and Masson staining were performed to evaluate pathological changes in the liver. Enzyme-linked immunosorbent assay (ELISA) was used to determine glutathione (GSH) and malondialdehyde (MDA) levels in the liver, and interleukin-1β (IL-1β), and IL-18 in serum. The levels of α-smooth muscle actin (α-SMA) and reactive oxygen species (ROS) in liver tissues were analyzed by immunohistochemistry and immunofluorescence, respectively. Western blot (WB) was used to analyze the expression levels of nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4), nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3), IL-1β, IL-18, caspase-1, and collagen I in liver. Finally, <i>in vitro</i> experiments were conducted using hepatic stellate cells (HSC-T6).</p><p><strong>Results: </strong>THHZD improved the pathological changes caused by CCl₄ in the liver. Compared with the normal group, the levels of serum ALT, AST, TBIL, IL-1β, and IL-18 in the model group were significantly increased. NOX4, ROS, NLRP3, IL-1β, IL-18, caspase-1, collagen I, α-SMA, and MDA levels were increased in the liver, while GSH levels were decreased. The administration of THHZD to CCl4-exposed rats significantly reversed or eliminated the changes in the above-mentioned indicators. Moreover, THHZD inhibited transforming growth factor-β1 (TGF-β1)-induced high expression of α-SMA and collagen I in HSC-T6 cells, while downregulating the expression of proteins related to the NOX4/NLRP3 signaling pathway <i>in vitro</i>.</p><p><strong>Conclusion: </strong>THHZD reduced the activation of HSCs by inhibiting the NOX4/NLRP3 signaling pathway, thereby exerting anti-hepatic fibrosis effects.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 4","pages":"873-882"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13494871/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148814867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antihypertensive effect of acupuncture involve reshaping gut microbiota and alleviating gut inflammation in spontaneously hypertensive rats.","authors":"Zheng Jie, Ding Zhimin, Chen Tingyu, Lü Zhuan, Zhang Lihong, Liu Yongtao, Zhang Yasu, Jin Xiaoqin, Wang Xinyu, Liu Bin, Feng Xiaodong","doi":"10.19852/j.cnki.jtcm.2026.04.011","DOIUrl":"10.19852/j.cnki.jtcm.2026.04.011","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the antihypertensive effect and mechanism of acupuncture at Renying (ST9) and Zusanli (ST36) from the perspective of gut microbiota.</p><p><strong>Methods: </strong>Eighteen male spontaneously hypertensive rats (SHRs) were randomly divided into the SHR group, the acupuncture group (SHR + A), the sham acupuncture group (SHR + SA), and six Wistar-Kyoto rats were set up as the normotensive control group. 16S ribosomal ribonucleic acid gene sequencing was performed to characterize changes in the gut microbiota. Blood samples were collected to measure inflammatory cytokines using enzyme-linked immunosorbent assay. Hematoxylin and eosin staining was used to assess the degree of colonic damage. Western blotting was performed to evaluate intestinal inflammation.</p><p><strong>Results: </strong>We observed that acupuncture at acupoints Renying (ST9) and Zusanli (ST36) reduced blood pressure in SHRs, modulated microbial beta diversity, increased the abundance of beneficial genera, and reshaped the composition of the intestinal microbiota. In addition, hypertension induced pathological changes in the intestine, along with inflammation and increased permeability. By contrast, acupuncture treatment decreased serum inflammatory cytokine levels and inhibited activation of the toll-like receptor 4/ nuclear factor-kappa B signaling pathway in SHRs, thereby restoring intestinal barrier integrity.</p><p><strong>Conclusions: </strong>Our experimental results indicate that acupuncture exerts a hypotensive effect that is associated with modulation of intestinal inflammation and microbiota, which may offer a new strategy for the prevention and treatment of hypertension.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 4","pages":"907-915"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13495073/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148815321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Baihe Dihuang decoction activates cannabinoid type 2 receptor to regulate microglial polarization and alleviate anxiety-like behavior in chronic restraint stress mice.","authors":"Luo Qiaoyun, Han Yuanshan, Liu Yang, Tang Lin, Wang Tianyu, Wang Yuhong, Zhao Hongqing","doi":"10.19852/j.cnki.jtcm.2026.04.003","DOIUrl":"10.19852/j.cnki.jtcm.2026.04.003","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the anxiolytic effects of Baihe Dihuang decoction (, BDD) and its underlying molecular mechanisms, focusing on cannabinoid type 2 receptor (CB2R)-mediated regulation of microglial polarization and the toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (MyD88)/nuclear factor-kappa B (NF-κB) signaling pathway.</p><p><strong>Methods: </strong>A total of 60 male C57BL/6J mice were randomly divided into six groups (<i>n</i>= 10 per group): control group, model group, diazepam (2 mg/kg) group, high-dose BDD (16 g/kg) group, low-dose BDD (8 g/kg) group, and high-dose BDD (16 g/kg) combined with CB2R antagonist AM630 (1 mg/kg) group. Except for the control group, all other groups were subjected to chronic restraint stress (CRS) to establish an anxiety model. Anxiety-like behaviors were assessed using the open field test, light-dark box test, and elevated plus maze. Serum levels of interleukin (IL)-1β, IL-6, IL-10, and IL-4 and amygdala concentrations of 2-arachidonoylglycerol (2-AG) and anandamide (AEA) were quantified by enzyme-linked immunosorbent assay. Hematoxylin and eosin, Nissl, and terminal deoxynucleotidyl transferase dUTP nick-end labeling staining were used to evaluate neuronal damage in the amygdala. Immunofluorescence staining was employed to assess microglial polarization and CB2R expression. The expression levels of CB2R and downstream signaling molecules TLR4/MyD88/NF-κB in the amygdala were determined by quantitative polymerase chain reaction and Western blot.</p><p><strong>Results: </strong>BDD significantly alleviated CRS-induced anxiety-like behaviors and reduced neuronal damage in the amygdala. It downregulated pro-inflammatory cytokines IL-1β, IL-6 and upregulated anti-inflammatory cytokines IL-10, IL-4 in serum while increasing 2-AG and AEA levels in the amygdala. Furthermore, BDD promoted microglial polarization toward the M2 phenotype and enhanced CB2R expression, while suppressing the TLR4/MyD88/NF-κB signaling pathway. The administration of the CB2R antagonist AM630 reversed these effects, confirming the essential role of CB2R activation in the anxiolytic and neuroprotective effects of BDD.</p><p><strong>Conclusion: </strong>BDD treatment activated CB2R, which in turn modulated microglial polarization in the amygdala <i>via</i>the TLR4/MyD88/NF-κB signaling pathway, thereby alleviating anxiety-like behaviors and exerting neuro-protective effects.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 4","pages":"798-808"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13494881/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148815373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroacupuncture enhances CD8+ T cells recruitmentmodulating sympathetic nervous system to suppress tumor growth in mice bearing triple-negative breast cancer.","authors":"Tian Yehong, Zhang Qiaoli, Zhang Rui, Gao Tao, L I Nanzhi, Huang Jinchang, Jiang Xin, Qiu Xiaowei","doi":"10.19852/j.cnki.jtcm.2026.04.006","DOIUrl":"10.19852/j.cnki.jtcm.2026.04.006","url":null,"abstract":"<p><strong>Objective: </strong>To investigate whether peritumoral electroacupuncture (EA) suppresses tumor growth <i>via</i>CD8+ T cell infiltration mediated by sympathetic nervous system (SNS) regulation.</p><p><strong>Methods: </strong>Utilizing a mouse model of triple-negative breast cancer (TNBC), the SNS was activated through chronic restraint stress (CRS); chemical sympathetic denervation was induced by 6-hydroxydopamine (6-OHDA). The anti-tumor and immune regulatory effects of EA were evaluated by multidimensional methods, including <i>in vivo</i>bioluminescence imaging, flow cytometry, immunofluorescence, and enzyme-linked immunosorbent assay.</p><p><strong>Results: </strong>Peritumoral EA decreased the volume and weight of tumors within 22 d after the implantation. Intratumoral CD8+ T cells significantly increased after EA. C-C motif chemokine ligand 5, as a chemokine that recruits effector T cells to the tumor microenvironment, also up-regulated. Meanwhile, EA reduced intratumoral sympathetic innervation and norepinephrine level. Subsequently, we found CRS significantly accelerates tumor growth and reduces the density of CD8+ T cell infiltration. However, EA reversed the tumor-promoting effect and immune suppression caused by CRS effectively. Furthermore, chemical sympathetic ablation with 6-OHDA revealed that the anti-tumor effect of EA was dependent on sympathetic integrity, as the ablation impaired both tumor-suppressing effect and CD8+ T cell recruitment mediated by EA.</p><p><strong>Conclusion: </strong>EA enhances anti-tumor immunity through SNS emphasizing the effective remodeling of the tumor immune microenvironment in mice with TNBC by neuro-immune interactions.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 4","pages":"832-843"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13494874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148815437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Healing effects of Traditional Chinese Medicine plaster Heiyu Gao on refractory diabetic foot ulcers: a case series of ultra-elderly patients with surgical contraindications.","authors":"Zhang Danyu, W U Chengxiang, W U Yu, G U Jingyu","doi":"10.19852/j.cnki.jtcm.2026.04.021","DOIUrl":"10.19852/j.cnki.jtcm.2026.04.021","url":null,"abstract":"<p><p>Debridement surgery and revascularization are essential procedures for treating refractory diabetic foot ulcers (DFUs). However, a significant therapeutic gap exists for patients deemed at prohibitively high surgical risk. We report a case series of three nonagenarian patients (aged 95, 94, and 96 years) with Wagner grade 4 DFUs in whom conventional treatments had failed and surgery was absolutely contraindicated. Two proprietary Traditional Chinese Medicine (TCM) black plasters, designated as Heiyu Gao A and B based on century-old formulations, were externally applied to the ulcers and surrounding skin. The uniqueness of these cases lies in their extraordinary clinical challenges and remarkable treatment outcomes. Complete ulcer healing was achieved in all patients within 27, 105, and 96 d, respectively, without requiring revascularization or surgical debridement. Treatment demonstrated two novel features: tissue-intelligent selective autolytic debridement targeting only necrotic tissue, and concurrent regenerative repair at wound margins. All patients experienced significant pain reduction within three days of initiating treatment. The healing was characterized by absence of scarring and restoration of normal skin architecture. The follow-up periods ranged from 95 to 580 d, with no recurrence observed. These findings suggest that TCM black plasters may offer an effective alternative for high-risk patients currently relegated to palliative care pathways.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 4","pages":"1018-1025"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13494890/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148815392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Traditional Chinese Medicine related factors of planned dialysis in chronic kidney disease stage 5 patients with type 2 diabetes mellitus: a multicenter cross-sectional study.","authors":"Zhang Ruimin, L I Shuang, Q U Yilun, Wang Qian, Xie Huidi, Wang Xiaolong, Duan Shuwei, W U Jie, Wang Yong, Dong Zheyi, Chen Xiangmei","doi":"10.19852/j.cnki.jtcm.2026.04.018","DOIUrl":"10.19852/j.cnki.jtcm.2026.04.018","url":null,"abstract":"<p><strong>Objective: </strong>To clarify the distribution of Traditional Chinese Medicine (TCM) symptoms, syndrome elements and complications in chronic kidney disease stage 5 (CKD5) patients with type 2 diabetes mellitus (T2DM), and to analyze the correlation factors between dialysis and non-dialysis patients, so as to provide reference for clinical syndrome differentiation and treatment.</p><p><strong>Methods: </strong>The general information, laboratory indicators, and TCM syndrome factors of CKD5 patients with T2DM were obtained upon admission. The participants were divided into non-dialysis and dialysis groups according to the treatment methods used. Multivariate logistic regression was used for analysis. The probability of planned dialysis in CKD5 patients with T2DM was calculated by combining multiple TCM syndrome factors, and a receiver operating characteristic curve was drawn.</p><p><strong>Results: </strong>Approximately 77.2% of the study participants were men, with a median age of 59 years. A total of 277 and 95 patients comprised the non-dialysis and dialysis groups, respectively. The median duration of dialysis was 49.6 d. The dialysis group had a significantly higher serum creatinine level; a significantly lower estimated glomerular filtration rate, serum uric acid level, and 24-h urinary protein level; and a significantly lower proportion of patients with atherosclerosis (<i>P <</i> 0.01). The frequencies of Water stagnation pattern, Damp retention pattern, Heart deficiency pattern, and Lung deficiency pattern in the dialysis group were significantly higher (<i>P <</i> 0.01，<i>P</i> < 0.05), while the frequencies of Phlegm turbidity pattern and Kidney deficiency pattern were significantly lower (<i>P <</i> 0.01). Male sex [odds ratio (<i>OR</i>) = 1.780], Heart deficiency pattern (<i>OR</i> = 3.714), <i>Yin</i>deficiency pattern (<i>OR</i> = 2.262), and Water stagnation pattern (<i>OR</i> = 1.984) were factors related to planned dialysis in CKD5 patients with T2DM. The area under the receiver operating characteristic curve for planned dialysis in CKD5 patients with T2DM was 73.8% [95% confidence interval (0.680, 0.797), <i>P</i> < 0.001].</p><p><strong>Conclusions: </strong>Male sex, Heart deficiency pattern, <i>Yin</i>deficiency pattern, and Water stagnation pattern were correlated with planned dialysis in CKD5 patients with T2DM. Kidney deficiency pattern and Phlegm turbidity pattern were correlated with CKD5 patients with T2DM who had not yet started planned dialysis. The study of TCM syndrome elements provides therapeutic ideas for relieving the uncomfortable symptoms of CKD 5 patients with T2DM, and provides reference for clinical syndrome differentiation and treatment.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"46 4","pages":"982-989"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13494870/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148814991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}