Journal of the American Association for Laboratory Animal Science : JAALAS最新文献

{"title":"The Effect of Capromorelin and Extended-Release Buprenorphine on Body Weight in Guinea Pigs (Cavia porcellus).","authors":"Jennifer K Silvers, Sarah L W Norris, Elizabeth M Punger, Amanda Chisty","doi":"10.30802/AALAS-JAALAS-25-162","DOIUrl":"10.30802/AALAS-JAALAS-25-162","url":null,"abstract":"<p><p>Guinea pigs (Cavia porcellus) are widely used in biomedical research and are sensitive to stress-induced anorexia and weight loss. Buprenorphine extended release (XR) is commonly used for analgesia in laboratory animals but has been associated with decreased appetite and weight loss. Capromorelin, a ghrelin receptor agonist, is FDA-approved to stimulate appetite in dogs and cats. However, its utility in mitigating opioid-associated weight loss in guinea pigs has not been evaluated. This study's primary objective was to evaluate the effects of capromorelin on body weight in guinea pigs, both independently and in conjunction with buprenorphine XR administration, and whether oral capromorelin mitigates the weight loss associated with buprenorphine administration. A secondary objective was to evaluate habituation to voluntary consumption of an oral delivery puree and continued consumption of the puree with capromorelin added. Forty-eight female guinea pigs were randomized into 4 groups (n = 12/group) in a 2 × 2 factorial design. Animals were acclimated for 5 days to receive oral treatments, and all received either oral capromorelin (5 mg/kg) or sham treatment daily for 3 days, combined with either a single subcutaneous dose of buprenorphine XR (0.48 mg/kg) or saline. Body weights were recorded days -7 through -3 and days 0 through 5. All animals accepted oral treatments without the need for oral gavage. Guinea pigs receiving buprenorphine XR experienced significant weight loss compared with controls and were significantly less likely to return to baseline weight. Capromorelin treatment did not significantly affect weight change, and no interaction between capromorelin and buprenorphine XR was observed. Therefore, capromorelin at 5 mg/kg once a day for 3 days did not mitigate weight loss associated with buprenorphine XR administration in guinea pigs. These findings emphasize the need for species-specific evaluation of appetite stimulants and support the value of extended monitoring following administration of long-acting analgesics.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":" ","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13249302/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147319284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and Pharmacokinetics of 3 Sustained-Release Buprenorphine Formulations in Cairo Spiny Mice (Acomys cahirinus).","authors":"Diana E Hasler, Lorna M Dunn, Christopher D Fry, Tara L Martin, Gerry Hish, Patrick A Lester","doi":"10.30802/AALAS-JAALAS-25-141","DOIUrl":"10.30802/AALAS-JAALAS-25-141","url":null,"abstract":"<p><p>African spiny mice (Acomys spp.) are an emerging animal model for regeneration due to their remarkable healing capabilities. Defining characteristics of these species include fragile skin that sloughs easily and shedding of the tail skin when grabbed, making handling and administration of parenteral drugs difficult in conscious animals. In addition, many studies in spiny mice involve painful procedures. To our knowledge, there are no reports regarding analgesia in Acomys spp. This prospective study aimed to assess 3 sustained-release buprenorphine formulations-lipid-bound extended-release buprenorphine (XRB), polymeric sustained-release buprenorphine (SRB), and long-acting transdermal buprenorphine (TB)-in spiny mice. Adult male and female Cairo spiny mice (Acomys cahirinus) were included and administered 1 of the 3 treatments (XRB 3.25 mg/kg, SRB 1 mg/kg, or TB 20 mg/kg). Tail flick assays to assess nociception and terminal blood collection for pharmacokinetic analysis were performed at baseline and a set timepoint following treatment administration (1, 2, 4, 8, 24, 48, and 72 hours) (n = 3 of each sex per timepoint per treatment group). Additional animals underwent repeated serial tail flick assays at 1, 2, 4, 8, 24, 48, and 72 hours following the administration of treatment or sterile saline (n = 5-8 of each sex per treatment group). XRB displayed the longest duration at which mean plasma buprenorphine concentrations were >1 ng/mL, with the drug remaining above this level for 48-72 hours, compared with 24-48 hours in spiny mice receiving TB and 8 hours in those receiving SRB. On serial tail flick assays, the mean percentage of maximum possible efficacy was highest at all timepoints up to 72 hours in the TB group, followed by the XRB group, suggestive of greater analgesic efficacy. Several spiny mice receiving SRB developed ulcerative skin lesions within 24 hours of administration, so this treatment is not recommended in Acomys without additional evaluation. XRB and TB are promising analgesic therapies in spiny mice, and treatment selection should be based on whether duration of action or ease of application is the priority.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":" ","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13091606/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147319345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cuprizone in Peanut Butter: An Alternative Method of Cuprizone Administration to Model Demyelination of the Central Nervous System.","authors":"Brooke Hawker, Ethan Chiddicks, Jack Flanagan, Bronwen Connor, Amy McCaughey-Chapman","doi":"10.30802/AALAS-JAALAS-25-151","DOIUrl":"10.30802/AALAS-JAALAS-25-151","url":null,"abstract":"<p><p>Modeling disease in rodents is essential for preclinical research, particularly in the development of drugs and therapies. Traditional disease modeling approaches often rely on invasive and stressful procedures such as repeated injections and/or surgical approaches. These methods raise welfare concerns and can introduce stress-related variables that compromise model reliability. Cuprizone (CPZ) is a copper-chelating compound widely used to induce CNS demyelination in mice over a 6-week period via incorporation into ground rodent chow. However, the presumed adverse taste of CPZ reduces food intake, resulting in weight loss, inconsistent consumption, and variable levels of demyelination, complicating disease comparison. To address this, we proposed that mixing CPZ into peanut butter (CPZ-PB) would promote voluntary intake, standardize daily dosing, and minimize weight loss, while maintaining effective demyelination. Thirty mice were divided into control, CPZ-PB, or CPZ-chow groups and received treatment daily for 6 weeks. Body weight and CPZ intake were recorded daily. The effect of demyelination through mechanical allodynia was assessed via the dynamic plantar aesthesiometer. Immunohistochemical analysis of myelin proteins and astrocyte activation was conducted on the brain and spinal cord after 6 weeks of treatment. Voluntary consumption of CPZ-PB successfully ameliorated weight loss concerns and achieved consistent daily CPZ dosing. Behavioral and immunohistochemical analyses confirmed effective demyelination comparable to traditional CPZ-chow methods. This study introduces an improved CPZ administration strategy that enhances animal welfare and provides a more reliable model for demyelination research.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":" ","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13086187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146204277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacokinetics and Paw Withdraw Pressure in Guinea Pigs (Cavia porcellus) Treated with Transdermal Buprenorphine.","authors":"Shawn Kozlov, Selena M Guerrero-Martin, Grace Payne, Lon V Kendall","doi":"10.30802/AALAS-JAALAS-25-161","DOIUrl":"10.30802/AALAS-JAALAS-25-161","url":null,"abstract":"<p><p>The use of effective analgesia is a cornerstone of laboratory animal care, one that minimizes pain and distress while optimizing care and welfare. Guinea pigs are often used in biomedical research requiring appropriate pain management. In this study, we evaluated the pharmacokinetics and efficacy of transdermal buprenorphine (Zorbium), a commercial product labeled for use in cats. Guinea pigs (n = 12) received a transdermal dose of buprenorphine (2.7 mg per guinea pig), an average dose of 3.4 mg/kg, applied topically to the interscapular area. Plasma concentration, paw withdraw pressure (PWP) response, sedation scores, and body temperature were collected at 0, 0.25, 0.5, 1, 2, 4, 8, 12, 24, 48, 96, and 120 hours after application. Plasma concentrations peaked at 77.8 ng/mL at 1 hour and remained greater than the targeted 1.0 ng/mL for up to 120 hours, with a half-life of 46.7 hours. PWP peaked at 24 hours with 671.2 g of pressure, remained elevated through 48 hours at 364 g, and returned to baseline by 72 hours. Sedation scores (posture, ambulation, resistance to handling) were slightly elevated at 12 hours, body weight did not significantly change, and rectal temperatures remained normal at all time points. These results suggest that transdermal buprenorphine maintains a prolonged therapeutic plasma level and is an effective analgesic for up to 48 hours in guinea pigs, with minimal side effects. This formulation is an alternative to other buprenorphine formulations that would require less handling for guinea pigs.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":" ","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13251881/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146204315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Gross and Histopathologic Changes from Repeated Celiotomies in African Clawed Frogs (Xenopus laevis).","authors":"Darya Mailhiot, Rebecca Turcios, Genevieve Remmers, Jennifer Brazzell, George P Langan, Bridget M Clancy, Kelly Kries, Kerith R Luchins","doi":"10.30802/AALAS-JAALAS-24-097","DOIUrl":"10.30802/AALAS-JAALAS-24-097","url":null,"abstract":"<p><p>Repeated survival surgical oocyte collection in African clawed frogs (Xenopus laevis) is commonly performed to access high-quality, viable oocytes for research procedures and to reduce the number of animals used in research. Despite this common practice, there is no evidence-based limit for the total number of survival celiotomies that can be performed. To provide an improved reference, a retired colony of experimental frogs (n = 31) was euthanized for gross and histopathologic evaluation and then compared with 4 experimentally naive age-matched controls (n = 4). Experimental animals underwent 4-11 celiotomies (average 6 ± 2) and were between 180 and 1,646 days (average 448 ± 245.2 days) from their last surgery. Body weight, residual skin, and coelomic wall sutures were counted. Surgically incised skin and coelomic wall musculature were collected for histopathology. A cumulative skin score and a coelomic wall score were developed to reflect all histopathologic evidence of acute and chronic inflammation. The cumulative skin score was significantly predicted by the number of surgeries (P = 0.003), but not predicted by the number of skin sutures remaining (P = 0.14). The coelomic wall score was significantly predicted by the number of coelomic sutures placed (P < 0.001) and the number of surgeries (P < 0.001). Neither one of the histopathology scores correlated to the number of days since the last surgery (P > 0.2). As one would expect, increasing the number of surgeries and coelomic sutures correlated with the histopathologic finding of a chronic inflammatory process in the skin. Although a specific recommendation for a maximum number of surgeries could not be discerned, this study provides an improved reference for Xenopus survival surgical oocyte collection underscoring the recommendation to use absorbable sutures within the body cavity and to remove all external nonabsorbable sutures 10-14 days postoperatively.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":" ","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13086188/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146204488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing Local Anesthesia in Octopus bimaculoides to Provide Analgesia.","authors":"Joanna N Copio, Natalie G Schulz, Clifton W Ragsdale, Anthony J Cerreta, Allison M Ostdiek, Kerith R Luchins, George P Langan, Darya Mailhiot","doi":"10.30802/AALAS-JAALAS-25-172","DOIUrl":"10.30802/AALAS-JAALAS-25-172","url":null,"abstract":"<p><p>Despite the substantial growth of cephalopod research over the past 15 years, effective pain management for these animals remains largely unstudied, and no US federal regulations currently address cephalopod analgesia. This 2-phase study evaluated the feasibility and efficacy of lidocaine, bupivacaine, and extended-release bupivacaine injections for producing analgesia in juvenile Octopus bimaculoides in an established model of arm regeneration. Phase I served as a pilot to assess adverse effects, practicality, and histopathologic consequences of drug administration. Forty-nine animals were randomized to the saline control or treatment groups. Phase II assessed lidocaine, bupivacaine, and saline in an additional 34 animals, using behavioral metrics at multiple time points, including a posture and gait analysis during a walking assessment and mechanical allodynia evaluation via the von Frey filament test. Health indicators, such as weight change and respiratory rate, were also monitored. Finally, wound healing and arm regeneration were followed with photo documentation. Although all animals displayed statistically significant changes in arm posture and gait immediately postsurgery, these changes invariably resolved within 72 hours irrespective of treatment group. Moreover, health and regenerative outcomes did not differ significantly among the treatment groups. The failure of local anesthetic efficacy highlights the need for alternative analgesic regimes for octopus research.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":" ","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13086191/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147319368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparative Analysis of the Genetic Structures of Rhesus Macaques (Macaca mulatta) Kept in Enclosure-Based Systems and Semi-Natural Settings.","authors":"Sree Kanthaswamy","doi":"10.30802/AALAS-JAALAS-25-159","DOIUrl":"10.30802/AALAS-JAALAS-25-159","url":null,"abstract":"<p><p>This study compares 2 rhesus macaque populations, one captive (the California National Primate Research Center [CNPRC]) and one seminatural (the Cayo Santiago [CS] population), to evaluate the impact of colony management on genetic structure. While the CNPRC houses its 4,000-plus animals in different housing enclosures, CS has a seminatural, free-ranging population of ∼1,900 individuals. Pedigrees of 3,679 animals at the CNPRC and 2,147 animals at CS were analyzed to assess cross-generational genome uniqueness (GU), inbreeding coefficients, mean kinship (MK), founder equivalents, and founder genome equivalents. An estimated 30% of the 409 CS founders contributed to the present population, compared with <10% of the 2,980 founders and subsequently introduced animals at the CNPRC. Differences in pedigree structure and potential inbreeding risk, particularly at the CNPRC, indicate a more genetically subdivided population there than at CS. The CNPRC also exhibited a lower GU than CS, whereas the MK of both colonies showed a similar upward trend over the study period (2000-2024). Also revealed are significant differences in reproductive activity, lifespan, and the number of families per individual between the 2 colonies. There are challenges in maintaining captive-bred colonies, such as the CNPRC, where restricted gene flow and inbreeding lead to dramatic declines in genetic variability. Strategies such as equalizing reproductive success, implementing cross-fostering programs for infants, and introducing new animals are insufficient to combat inbreeding, genetic subdivisions, and the loss of founder representation. Findings highlight the need for ongoing monitoring of inbreeding coefficients, GU, and MK to safeguard genetic integrity and ensure the long-term viability of captive-bred colonies. The analysis also offers insights for managing these colonies, highlighting MK as a key metric for preserving genetic diversity because it integrates founder representation, drift, and inbreeding into a colony-wide measure that directly informs colony management decisions.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":" ","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13086203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146208434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Single-Operator Jugular Vein Sampling Method for Repeated Blood Collection in Conscious Rats.","authors":"Mingqiang Wang, Juncheng Deng, Yingyong Wen, Youan Xue, Yongjiao Xie, Yi Jin, Jing Li","doi":"10.30802/AALAS-JAALAS-25-164","DOIUrl":"10.30802/AALAS-JAALAS-25-164","url":null,"abstract":"<p><p>Jugular vein (JV) blood sampling is widely employed in pharmacological, toxicological, and pharmacokinetic studies due to its ability to provide large and repeatable blood volumes. However, current approaches are constrained by low convenience, safety concerns, and variable sample quality. Here, we report the development of a new single-operator JV blood sampling technique for conscious rats. By optimizing animal restraint, puncture site localization, and procedural standardization, the method significantly reduced sampling time, puncture attempts, pain-related behaviors, and hemorrhagic injury, while ensuring consistent blood yield. Compared with the conventional approach, it achieved a 100% success rate, halved the average sampling time, and maintained stable serum biochemical, coagulation, and hematologic parameters. The utility of this method was further confirmed in an intraperitoneal glucose tolerance test and a cisplatin-induced acute kidney injury model, where data consistency was preserved. This technique provides a convenient, safe, and reliable alternative for repeated blood collection in conscious rats, ensuring animal welfare and supporting the generation of high-quality data in preclinical research.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":" ","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13086199/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146138258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paired versus Single Housing of Ferrets (Mustela putorius furo) Does Not Influence Experimental Outcomes of Influenza A Virus Pathogenesis Studies.","authors":"Jessica A Belser, Troy J Kieran, Melissa A Truelove, Gregory Daggett, Sherrie M Jean, Taronna R Maines","doi":"10.30802/AALAS-JAALAS-25-191","DOIUrl":"10.30802/AALAS-JAALAS-25-191","url":null,"abstract":"<p><p>Social housing of animals is well recognized as a fundamental element in providing for the well-being of social animals during laboratory research activities but is contraindicated in some experimental designs. To ascertain if social housing represented a potential confounder in studies determining influenza A virus (IAV) pathogenicity conducted in the ferret model, we analyzed data from ferrets inoculated with 56 distinct IAV strains, where animals were housed either singly or in pairs postinoculation. Parameters examined included frequency of lethal outcomes, timing and magnitude of clinical signs (weight loss and fever), and magnitude and kinetics of viral shedding in the upper respiratory tract. Statistical differences between IAV-inoculated ferrets housed in either setting were not consistently detected among any parameters examined, supporting that data from ferrets inoculated with a diverse range of avian- and mammalian-origin IAV housed singly or in social pairs may be combined and analyzed without confounding based on this variable. This study supports the utility of performing retrospective analyses to ensure results obtained from in vivo experimentation are interpreted to the highest standard possible. Further, it aligns with ethical obligations of researchers who perform in vivo experimentation to ensure these studies are designed to meet both animal welfare considerations and research goals.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":" ","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13086212/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146208469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standardizing Perioperative Heat to Improve Mouse (Mus musculus) Recovery.","authors":"Andrew J Popadich, Bhanvi Mishra, Stephanie N Oldham, Seo-Kyoung Hwang, Xiaokang Luo, Julita A Ramirez, Erin E Straley, Robin J Kastenmayer","doi":"10.30802/AALAS-JAALAS-25-173","DOIUrl":"10.30802/AALAS-JAALAS-25-173","url":null,"abstract":"<p><p>Maintaining the core body temperature of anesthetized rodents is essential because of the depression of physiologic homeostasis caused by anesthetics. The maintenance of core body temperature is influenced by the ability of the heating device to provide sufficient heat, the presence of material that might alter heat transfer, and the administration of heat in the period surrounding anesthesia. In this study, optimal heat transfer from 4 unique heating devices, with or without insulating drapes, was determined initially with an inert model. Optimal animal recovery, as evaluated by recovery to baseline activity and normothermic temperature of postoperative animals implanted with an intrabdominal thermometer and monitored with a digitally ventilated caging, was achieved by a device that provided consistent electrically supplied heat at 40 °C throughout the perianesthesia period including 35 minutes before isoflurane anesthetic induction. Animals without preoperative heating required at least 24 hours to return to normal core temperature and normal circadian activity levels.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":" ","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13086213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146222562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}