牛冠状杆菌在组织培养条件和培养基中的生长。

Alyssa M Kleymann, Nicholas A Zawadzki, Derek L Fong, Michael K Fink, Lauren M Habenicht, Jori K Leszczynski, Steven M Anderson, Michael J Schurr, Christopher A Manuel
{"title":"牛冠状杆菌在组织培养条件和培养基中的生长。","authors":"Alyssa M Kleymann, Nicholas A Zawadzki, Derek L Fong, Michael K Fink, Lauren M Habenicht, Jori K Leszczynski, Steven M Anderson, Michael J Schurr, Christopher A Manuel","doi":"10.30802/AALAS-JAALAS-24-050","DOIUrl":null,"url":null,"abstract":"<p><p>A common concern in preclinical cancer research is the introduction of <i>Corynebacterium bovis</i> into immunodeficient mouse colonies through cancer cell lines. <i>C. bovis</i> is a known contaminant of patient-derived xenograft tumors passaged horizontally between immunodeficient mice. However, it is unclear if <i>C. bovis</i> can grow in mammalian tissue culture conditions or tissue culture media. We hypothesized that <i>C. bovis</i> would not grow under tissue culture conditions or media, diminishing the risk of transmission from tumor cell lines cultured <i>in vitro</i>. Three <i>C. bovis</i> isolates, CUAMC1, HAC, and ATCC-7715, were used to test our hypothesis in 3 of the most common media used to grow human cancer cell lines including RPMI 1640 + 10% FBS (RPMI), DMEM/high glucose + 10% FBS (DMEM), and DMEM/F-12 + 10% FBS (DMEM/F12). Our results confirmed propagation of each <i>C. bovis</i> isolate in DMEM/F12 media under tissue culture conditions after 72 h. However, these results also demonstrate diminished viability of each <i>C. bovis</i> isolate in RPMI and DMEM after 72 h. To assess whether antibiotics could halt the growth of <i>C. bovis</i> under tissue culture conditions in DMEM/F12, penicillin-streptomycin (pen/strep) was added to the experimental media. This treatment was effective in eliminating all viable <i>C. bovis</i> in the culture system after 72 h. Our data suggest that <i>C. bovis</i> growth under tissue culture conditions is possible and growth in tissue culture media is nuanced. These results highlight the importance of pathogen surveillance for tumor cell lines propagated <i>in vitro</i> and demonstrate the need for further investigation into <i>C. bovis</i> growth requirements.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"<i>Corynebacterium bovis</i> Growth in Tissue Culture Conditions and Media.\",\"authors\":\"Alyssa M Kleymann, Nicholas A Zawadzki, Derek L Fong, Michael K Fink, Lauren M Habenicht, Jori K Leszczynski, Steven M Anderson, Michael J Schurr, Christopher A Manuel\",\"doi\":\"10.30802/AALAS-JAALAS-24-050\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A common concern in preclinical cancer research is the introduction of <i>Corynebacterium bovis</i> into immunodeficient mouse colonies through cancer cell lines. <i>C. bovis</i> is a known contaminant of patient-derived xenograft tumors passaged horizontally between immunodeficient mice. However, it is unclear if <i>C. bovis</i> can grow in mammalian tissue culture conditions or tissue culture media. We hypothesized that <i>C. bovis</i> would not grow under tissue culture conditions or media, diminishing the risk of transmission from tumor cell lines cultured <i>in vitro</i>. Three <i>C. bovis</i> isolates, CUAMC1, HAC, and ATCC-7715, were used to test our hypothesis in 3 of the most common media used to grow human cancer cell lines including RPMI 1640 + 10% FBS (RPMI), DMEM/high glucose + 10% FBS (DMEM), and DMEM/F-12 + 10% FBS (DMEM/F12). Our results confirmed propagation of each <i>C. bovis</i> isolate in DMEM/F12 media under tissue culture conditions after 72 h. However, these results also demonstrate diminished viability of each <i>C. bovis</i> isolate in RPMI and DMEM after 72 h. To assess whether antibiotics could halt the growth of <i>C. bovis</i> under tissue culture conditions in DMEM/F12, penicillin-streptomycin (pen/strep) was added to the experimental media. This treatment was effective in eliminating all viable <i>C. bovis</i> in the culture system after 72 h. Our data suggest that <i>C. bovis</i> growth under tissue culture conditions is possible and growth in tissue culture media is nuanced. These results highlight the importance of pathogen surveillance for tumor cell lines propagated <i>in vitro</i> and demonstrate the need for further investigation into <i>C. bovis</i> growth requirements.</p>\",\"PeriodicalId\":94111,\"journal\":{\"name\":\"Journal of the American Association for Laboratory Animal Science : JAALAS\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-08-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of the American Association for Laboratory Animal Science : JAALAS\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.30802/AALAS-JAALAS-24-050\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the American Association for Laboratory Animal Science : JAALAS","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.30802/AALAS-JAALAS-24-050","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

临床前癌症研究中的一个常见问题是通过癌细胞株将牛棒状杆菌引入免疫缺陷小鼠群。已知牛海绵状杆菌是在免疫缺陷小鼠间水平传递的患者来源异种移植肿瘤的污染源。然而,目前还不清楚牛肝菌是否能在哺乳动物组织培养条件或组织培养基中生长。我们假设牛海绵状芽孢杆菌不会在组织培养条件或培养基中生长,从而降低了从体外培养的肿瘤细胞系中传播的风险。我们用 CUAMC1、HAC 和 ATCC-7715 这三种牛海绵状芽孢杆菌分离物在 3 种最常用的培养人类肿瘤细胞系的培养基(包括 RPMI 1640 + 10% FBS (RPMI)、DMEM/高葡萄糖 + 10% FBS (DMEM) 和 DMEM/F-12 + 10% FBS (DMEM/F12))中测试了我们的假设。为了评估抗生素是否能阻止牛杆菌在 DMEM/F12 组织培养条件下的生长,我们在实验培养基中添加了青霉素-链霉素(pen/strep)。我们的数据表明,牛海绵状芽孢杆菌在组织培养条件下是可以生长的,而且在组织培养基中的生长是有细微差别的。这些结果突显了对体外繁殖的肿瘤细胞系进行病原体监控的重要性,并表明有必要进一步研究牛肝菌的生长要求。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Corynebacterium bovis Growth in Tissue Culture Conditions and Media.

A common concern in preclinical cancer research is the introduction of Corynebacterium bovis into immunodeficient mouse colonies through cancer cell lines. C. bovis is a known contaminant of patient-derived xenograft tumors passaged horizontally between immunodeficient mice. However, it is unclear if C. bovis can grow in mammalian tissue culture conditions or tissue culture media. We hypothesized that C. bovis would not grow under tissue culture conditions or media, diminishing the risk of transmission from tumor cell lines cultured in vitro. Three C. bovis isolates, CUAMC1, HAC, and ATCC-7715, were used to test our hypothesis in 3 of the most common media used to grow human cancer cell lines including RPMI 1640 + 10% FBS (RPMI), DMEM/high glucose + 10% FBS (DMEM), and DMEM/F-12 + 10% FBS (DMEM/F12). Our results confirmed propagation of each C. bovis isolate in DMEM/F12 media under tissue culture conditions after 72 h. However, these results also demonstrate diminished viability of each C. bovis isolate in RPMI and DMEM after 72 h. To assess whether antibiotics could halt the growth of C. bovis under tissue culture conditions in DMEM/F12, penicillin-streptomycin (pen/strep) was added to the experimental media. This treatment was effective in eliminating all viable C. bovis in the culture system after 72 h. Our data suggest that C. bovis growth under tissue culture conditions is possible and growth in tissue culture media is nuanced. These results highlight the importance of pathogen surveillance for tumor cell lines propagated in vitro and demonstrate the need for further investigation into C. bovis growth requirements.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信