Journal of the American Association for Laboratory Animal Science : JAALAS最新文献

{"title":"The Incidence of Volatile Anesthesia Porcine Stress Syndrome in Pigs (Sus scrofa domesticus) Gives Implications for Physiology during Anesthesia.","authors":"James Corrigan, John Mares, Justin Hutzler, Dan Nonneman, David M Burmeister","doi":"10.30802/AALAS-JAALAS-24-077","DOIUrl":"10.30802/AALAS-JAALAS-24-077","url":null,"abstract":"<p><p>Pigs are extensively used for biomedical research as animal models given their similarities to humans including size, arterial capacity, and cutaneous structure. While their size also allows for the use of clinically available anesthesia equipment (for example, endotracheal tubes and ventilators), anecdotes exist with respect to stress reactions after exposure to volatile anesthetics. Over 3 mo at our institution, 11 pigs (Sus scrofa domesticus) exposed to isoflurane anesthesia during 2 research protocols were euthanized after exhibiting clinical signs of malignant hyperthermia, including hyperthermia, hypercapnia, skeletal muscle rigidity, dyspnea, tachycardia, and hypotension. This group was composed of intact Yorkshire/Landrace crosses (68 to 91 kg) purchased from a research breeder. While malignant hyperthermia is caused by a mutation in ryanodine receptor 1 (RYR1), another unnamed porcine stress syndrome is caused by a dystrophin defect. We analyzed the incidence of the RYR1 mutation and a dystrophin variant in 9 of the originally clinically affected pigs and in 56 subsequent pigs. All animals tested negative for the RYR1 mutation, while the dystrophin variant was found in 2 out of 7 clinical (28.6%) and 22 out of 46 (47.8%) subsequently tested female pigs. Creatine kinase, indicative of muscle damage, was slightly elevated at baseline in dystrophin variant-positive carriers, albeit not significantly. However, for the original clinically affected pigs, the increase in body temperature while under anesthesia was significantly greater in dystrophin variant-positive carriers (7.9 ± 0.8 °C) compared with noncarriers (5.2 ± 0.6 °C, P = 0.046). Taken together, we describe the suspected involvement of a dystrophin variant as one of the genetic etiologies in an unnamed condition that has been anecdotally experienced by pig researchers but not reported. We propose naming this condition volatile anesthesia porcine stress syndrome (VAPSS), which is an umbrella term that includes multiple genetic origins, the most well-known of which is malignant hyperthermia stress syndrome in pigs. Identifying other etiologies for VAPSS has implications for genetic and clinical screening to improve welfare in pigs bred for biomedical research and agricultural purposes.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":"64 1","pages":"179-188"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11808369/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143545033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antidiabetic Effects and Mechanisms of Action of Uncaria gambir Roxb. in Diabetic Sprague-Dawley Rats.","authors":"Indah Fajarwati, Dedy D Solihin, Tutik Wresdiyati, Irmanida Batubara, Sela S Mariya","doi":"10.30802/AALAS-JAALAS-24-117","DOIUrl":"10.30802/AALAS-JAALAS-24-117","url":null,"abstract":"<p><p>The antidiabetic properties of Uncaria gambir are not yet fully understood, particularly concerning how it affects diabetic animal models. Further investigation in this aspect is pivotal before initiating clinical evaluations. This study aimed to investigate the antidiabetic activity of U. gambir and how it influences blood glucose levels in diabetic Sprague-Dawley rats. In this study, 28 rats were divided into 7 groups. The groups were as follows: a nondiabetic rat group, a nondiabetic rat group given U. gambir, a diabetic rat group, a diabetic rat group given glibenclamide, and 3 diabetic rat groups given U. gambir at 3 doses (200, 300, and 400 mg/kg). Diabetes was induced using streptozotocin (50 mg/kg) given by intraperitoneal injection. Blood glucose levels were measured weekly, and the animals were euthanized at the end of the experiment. Intracardiac blood and tissues such as the pancreas, liver, and skeletal muscle were collected for further analysis. The results showed that administering U. gambir to diabetic rats resulted in significantly lower blood glucose levels than untreated diabetic rats. U. gambir has a complex mechanism to reduce blood glucose levels. including increase of insulin production, preservation of the islets and pancreatic β cells, and optimization of glycogenesis, as reflected in a significant increase in liver glycogen levels. These findings suggest that U. gambir's multicompound and multitarget capabilities in controlling blood glucose levels may have utility for treatment of diabetes.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":"64 1","pages":"35-43"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11808383/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143545427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infrared Thermography for Temperature Measurement in Adult Female C57BL/6NCrl Mice: A Comparison with Rectal Probe and Subcutaneous Transponder.","authors":"Kerstin Fiebig, Thomas Jourdan, Martin Kock, Karolina Krehl, Roswitha Merle, Christa Thöne-Reineke","doi":"10.30802/AALAS-JAALAS-23-000114","DOIUrl":"10.30802/AALAS-JAALAS-23-000114","url":null,"abstract":"<p><p>Body temperature is an easily measured clinical parameter that provides important information about an animal's health and welfare. In the context of animal experiments, temperature monitoring provides relevant data needed to manage animal care and has been embraced as a means of assessing humane endpoints. At the same time, temperature measurement in the sense of the 3Rs (Replacement, Refinement, and Reduction) should not cause any additional pain or distress to the animals. Therefore, the use of noninvasive, accurate, and cost-effective methods for temperature monitoring is of great importance in research laboratories. The purpose of this study was to determine the accuracy and consistency of different temperature measurement methods in black-haired C57BL/6NCrl mice. Body surface temperature measured by noninvasive infrared thermography was compared with established methods: subcutaneous and rectal temperature measurements. The study was conducted on 50 adult female mice, and measurements were taken for 5 d. Temperatures were measured using previously implanted subcutaneous temperature transponders, followed by infrared thermometry and rectal probes. The analyzed data showed that mouse temperature measurement using an infrared camera is an adequate method for noncontact and noninvasive temperature assessment in female C57BL/6NCrl mice and promotes laboratory animal welfare refinement.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":"64 1","pages":"120-131"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11808375/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143545488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Antimicrobial Therapy in Eradicating Chlamydia muridarum in Research Mice: Immune Status and Its Impact on Outcomes.","authors":"Michael B Palillo, Sebastian E Carrasco, Noah Mishkin, Jack A Palillo, Denise B Lynch, Samira Lawton, Mert Aydin, Anthony Mourino, Neil S Lipman, Rodolfo J Ricart Arbona","doi":"10.30802/AALAS-JAALAS-24-069","DOIUrl":"10.30802/AALAS-JAALAS-24-069","url":null,"abstract":"<p><p>Chlamydia muridarum (Cm) is a moderately prevalent, gram-negative, intracellular bacterium that affects laboratory mice, causing subclinical to severe disease, depending on the host's immune status. The effectiveness of various antibiotic regimens aimed at eradicating Cm in both immunodeficient and immunocompetent laboratory mice was evaluated. NSG mice were cohoused with Cm-shedding BALB/cJ mice for 14 d to simulate natural exposure. Four groups of 8 infected NSG mice were treated for 7 d with either 0.08% sulfamethoxazole and 0.016% trimethoprim (TMS) in water, 0.0625% doxycycline in feed, 0.124%/0.025% TMS in feed, or 0.12% amoxicillin in feed. A control group was provided standard water and feed. The impact of treatment on gastrointestinal microbiota (GM) was investigated using next-generation shotgun sequencing on the last day of treatment. TMS and amoxicillin had negligible effects on GM, while doxycycline had the largest effect. All antibiotic-treated NSG mice exhibited clinical disease, including dehydration, hunched posture, greater than 20% weight loss, and dyspnea, leading to euthanasia 21 to 40 d posttreatment (32.6 ± 4.2 d; mean ± SD). Untreated controls were euthanized 14 to 33 d postexposure (23.75 ± 5.9 d). All mice were fecal PCR positive for Cm at euthanasia. Histologic evaluation revealed multifocal histiocytic and neutrophilic bronchointerstitial pneumonia and/or bronchiolitis featuring prominent intralesional chlamydial inclusion bodies in all mice. Subsequently, groups of 8 C57BL/6J, BALB/cJ, NOD.SCID, and NSG mice infected with Cm were treated with 0.124%/0.025% TMS in feed for 7 (BALB/cJ and C57BL/6J) or 21 d (NSG and NOD.SCID). All immunocompetent and NOD.SCID mice were negative for Cm by PCR 14 d posttreatment, remained clinically normal, and had no evidence of Cm infection at necropsy, and all NSG mice remained Cm positive and were euthanized. While these findings highlight the difficulties in eradicating Cm from highly immunodeficient mice, eradication of Cm from immunocompetent or moderately immunocompromised mice with antibiotics is feasible.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":"64 1","pages":"76-88"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11808367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143545429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Overview of Anesthetic Procedures for Ferret (Mustela putorius furo) Preclinical Brain MRI: A Call for Standardization.","authors":"Diego Celdran-Bonafonte, Kathryn A O'Connell, Laurel Dieckhaus, Emily Mocarski, Natalie M Adras, Elisabeth B Hutchinson, Cholawat Pacharinsak","doi":"10.30802/AALAS-JAALAS-24-086","DOIUrl":"10.30802/AALAS-JAALAS-24-086","url":null,"abstract":"<p><p>Neuroscience research has been increasingly active during the last 2 decades, largely driven by the high prevalence and increasing burden of neurologic disorders. While rodents are essential models for biomedical research of neurologic disorders, they lack similar brain anatomy and protein expression profiles to humans, thus limiting their translational value as models of traumatic brain injury, stroke, Alzheimer's disease, and Parkinson's disease. The use of ferrets as a model species in neuroscience has been increasing due to their neuroanatomical similarities with humans, including a gyrencephalic brain and larger white matter-to-gray matter ratio. Compared with nonhuman primates, sheep, and swine, ferrets also have the advantages of reduced size, lower housing costs, and lower phylogenetic order. These, among other advantages, make ferrets a promising species to bridge research gaps and complement traditional rodent studies. Although modern neuroscience research in ferrets relies heavily on advanced imaging techniques such as magnetic resonance imaging (MRI), most of the preclinical MRI installations are not designed or optimized for use with ferrets. In this paper we discuss the intricacies and limitations that need to be considered when performing ferret MRI procedures. Reviews of ferret biologic peculiarities, anesthetic protocols, management of complications, and the impact of these factors on MRI outcomes are presented. Standardizing anesthesia protocols for the conduct of MRI in ferrets will aid in better physiologic monitoring as well as imaging outcomes that ultimately benefit the science being conducted.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":"64 1","pages":"16-28"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11808380/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143545471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental Enrichment Attenuates Reproductive Adversity in a Mouse Model of Parkinson Disease.","authors":"Courtney A Walsh, Lindsay Johnson, Patricia A Cirillo, Peter C Smith, Steven R Wilson","doi":"10.30802/AALAS-JAALAS-24-000032","DOIUrl":"10.30802/AALAS-JAALAS-24-000032","url":null,"abstract":"<p><p>Environmental enrichment is the provision of different substrates to mimic an animal's natural environment and encourage natural, species-specific behavior. However, the use of enrichment to improve breeding efficiency in mouse models for neurologic conditions is not well described. There are reports that diminished environmental stimuli and chronic isolation can result in the early expression of the Parkinson phenotype in mice with a genetic predisposition to the disease. In this study, we compared the provision of crinkle paper, DietGel, and their combination on reproductive parameters in B6.Cg-Tg(THY1-SNCA*A53T)M53Sud mice. We found that enhanced enrichment combined with enhanced nutrition increased dam weight and decreased the interlitter intervals. In addition, enhanced enrichment increased the production index, number of pups born, pups weaned, and the percent survival of pups. This study underscores the importance of incorporating enrichment to enhance the reproductive parameters in mice that are models of Parkinson disease.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":"64 1","pages":"58-63"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11808379/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143545475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Food Marking and Contamination Behaviors Informs Feeding Practice in Research Colonies of Laboratory Opossums.","authors":"Abbigail L Lanier, Bettina Schemera, Patricia E Rynders, Trisha Kendall, Myra Petrofski, Jennifer Spooner, Paul B Samollow, John L VandeBerg, Xu Wang","doi":"10.30802/AALAS-JAALAS-24-060","DOIUrl":"10.30802/AALAS-JAALAS-24-060","url":null,"abstract":"<p><p>The laboratory opossum, Monodelphis domestica, serves as a critical marsupial model in biomedical research. Proper feeding approaches are essential for promoting animal growth and wellbeing. In this study, we systematically evaluated food scattering and potential food contamination from feces across 4 feeding methods: direct placement of food pellets on bedding and using 3 different types of containers. We conducted timed daily observations of food scattering and marking behaviors in 22 animals, capturing images by photograph at specific intervals over the course of a week. Body weight was measured before and after the trial. Our findings revealed that the containers did not prevent food scattering behaviors, as evidenced by comparable survival curves for food scattering across all methods (P > 0.05, log-rank test). Although the paper tray and ceramic dish delayed the occurrence of food marking by feces, indicated by a significant extension in the time to marking events (P = 0.009 and P < 0.001, respectively), these containers introduced new animal welfare concerns. The paper tray increased bleeding incidents in digits and paw pads nearly 8-fold (P = 0.0002), presumably due to sharp edges. The ceramic dish was associated with urine marking, and small but statistically significant weight loss (0.7%, P < 0.05). By 144 h, all cages showed food contamination regardless of the feeding method. The results suggest that containers provide minimal benefit in preventing food contamination, and some types of containers may pose health risks. Therefore, we propose that placing food pellets directly on the bedding, a practice used for 45 y of laboratory opossum maintenance, is acceptable for promoting optimal health and operational efficiency for this species. Our results fill a significant gap in care practices and offer insights into optimal colony management for this important research model.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":"64 1","pages":"89-97"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11808382/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143545498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light Cycle Phase Effects on Behavioral Responses to Stress Exposure in Rats.","authors":"Chad E Brown, Andrew C Borrell, James C DeMar, Cooper J Almarode, Aurian O Naderi, Abraham J Han, Rachel M Taylor, Emily G Lowery-Gionta","doi":"10.30802/AALAS-JAALAS-24-103","DOIUrl":"10.30802/AALAS-JAALAS-24-103","url":null,"abstract":"<p><p>Animal models enable investigation of the impact of stress on emotional-behavioral performance to facilitate understanding of posttraumatic stress symptoms experienced in humans. Refinement of animal stress models could lead to a reduction in the number of subjects needed to detect statistically significant stress effects, in accordance with Russel and Burch's three Rs of research. We assessed whether performance of experimental procedures (that is, stress exposure and poststress behavioral testing) during the dark or light phases of the 12-h light/12 h-dark cycle is a refinement that could accomplish this reduction. At 3 h into either the light or dark phase, male and female adult Sprague-Dawley rats underwent a single-day traumatic stress exposure. Rats then underwent behavioral testing for exploratory behaviors, startle responses, and conditioned fear responses at 2 h, 1 d, and 9 d after stress exposure. Distance traveled in the elevated plus maze (EPM) by both male and female rats was significantly reduced in the dark phase compared with the light phase. Male rats of the dark phase group also spent less time in the open arms of the EPM, and traveled less, spent less time in the center, and spent more cumulative time freezing in the open field. Female rats of the dark phase group spent more cumulative time freezing in the EPM and exhibited significantly more tone-cued conditioned freezing responses. Our results suggest that performing experimental procedures during the dark phase of the light cycle may be a useful refinement mechanism, as procedures performed during this period had the greatest effect on behavioral outcomes in both males and females. Light cycle phase is an experimental variable that should be considered when designing experiments to maximize behavioral effects, including those in response to stress.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":"64 1","pages":"64-75"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11808373/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143545503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nerve Sheath Tumor in a Cynomolgus Macaque (Macaca fascicularis) and Diagnostic Differentiation from Amelanotic Melanoma.","authors":"Lisa C Mullaney, Johanna Rigas, Christine Watson, Anil Puttaswamy, Lisa Haviland, Laure Molitor, Elise E B LaDouceur","doi":"10.30802/AALAS-JAALAS-24-057","DOIUrl":"10.30802/AALAS-JAALAS-24-057","url":null,"abstract":"<p><p>There are few reports describing spontaneous neoplasms in cynomolgus macaques, despite the frequent use of this species in laboratory research. This report describes cytologic, histologic, and immunohistochemical findings of a cutaneous to subcutaneous nerve sheath tumor located within the haired skin of the abdomen of a 2.5-y-old, intact, female, captive Mauritius cynomolgus macaque. The nerve sheath tumor was well demarcated, partially encapsulated, densely cellular, and extended from the subcutis to the most superficial dermis, abutting the epidermis. Neoplastic cells formed intersecting streams and had a high mitotic count (18 per 2.37 mm2). Due to the substantial morphologic overlap of this neoplasm with amelanotic melanoma, particularly the close association with the epidermis, immunohistochemistry was required for definitive diagnosis. Neoplastic cells were immunoreactive to vimentin, S-100, SOX10, laminin, collagen IV, and CD56, and negative for melan-A, tyrosinase, MITF, and HMB45. This immunohistochemical profile is diagnostic for nerve sheath tumor based on human and canine criteria and rules out amelanotic melanoma. Despite incomplete excision, the nerve sheath tumor had not grossly recurred after 1 mo, at which point the animal was euthanized for unrelated reasons. This report underscores the importance of using an immunohistochemical panel in cases of cutaneous and subcutaneous spindle cell neoplasms, as there is substantial morphologic and immunohistochemical overlap between nerve sheath tumors and melanocytic neoplasms due to their shared neuroectodermal origin. To our knowledge, this is the first report of a nerve sheath tumor in a cynomolgus macaque, and one of the few reports of spontaneous neoplasia in this species.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":"64 1","pages":"44-48"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11808374/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143545518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effective Eradication of Mouse Norovirus and Helicobacter spp. in Laboratory Mice (Mus musculus) via Iodine Immersion and Cross-Fostering Technique.","authors":"Roseann E Kehoe, David C Reimer, Lauren A Bright, Bhupinder Singh, Jeetendra Eswaraka","doi":"10.30802/AALAS-JAALAS-24-087","DOIUrl":"10.30802/AALAS-JAALAS-24-087","url":null,"abstract":"<p><p>Eradication of pathogens from mouse colonies is crucial for scientific research reproducibility and animal welfare. The previously described techniques for eradicating pathogens by rederivation through embryo transfer or caesarian technique can be costly and technically challenging. The objective of our study was to assess the efficacy of iodine immersion combined with cross-fostering for eradicating murine norovirus (MNV) and Helicobacter spp. in laboratory mice. The iodine immersion technique was modified to prevent pathogen transmission and reduce the risk of cannibalism. The hypothesis of this study was that iodine immersion combined with cross-fostering of pups would be as effective at eliminating MNV as it is at clearing Helicobacter in laboratory mice. This study was performed on newborn litters of various mice strains housed in a room positive for both these pathogens. The pups were immersed in warmed, diluted iodine within 48 h of birth, and then cross-fostered to a Swiss Webster dam negative for MNV and Helicobacter. The presence of MNV and Helicobacter in donor dams, weanlings, and adult post-immersion animals was tested using fecal PCR. All 27 litters born to MNV- and Helicobacter-positive dams tested negative for both pathogens at weaning and at 8 wk postweaning. Follow-up PCR exhaust dust testing from the housing racks confirmed a negative status for MNV and Helicobacter over multiple quarterly pathogen screening tests conducted over an 18-mo period. This study is, to our knowledge, the first to demonstrate successful eradication of MNV through iodine immersion combined with cross-fostering, proving this method to be effective for eliminating MNV and Helicobacter spp. in affected colonies.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":"64 1","pages":"29-34"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11808376/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143545436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}