Corynebacterium bovis Growth in Tissue Culture Conditions and Media.

Alyssa M Kleymann, Nicholas A Zawadzki, Derek L Fong, Michael K Fink, Lauren M Habenicht, Jori K Leszczynski, Steven M Anderson, Michael J Schurr, Christopher A Manuel
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Abstract

A common concern in preclinical cancer research is the introduction of Corynebacterium bovis into immunodeficient mouse colonies through cancer cell lines. C. bovis is a known contaminant of patient-derived xenograft tumors passaged horizontally between immunodeficient mice. However, it is unclear if C. bovis can grow in mammalian tissue culture conditions or tissue culture media. We hypothesized that C. bovis would not grow under tissue culture conditions or media, diminishing the risk of transmission from tumor cell lines cultured in vitro. Three C. bovis isolates, CUAMC1, HAC, and ATCC-7715, were used to test our hypothesis in 3 of the most common media used to grow human cancer cell lines including RPMI 1640 + 10% FBS (RPMI), DMEM/high glucose + 10% FBS (DMEM), and DMEM/F-12 + 10% FBS (DMEM/F12). Our results confirmed propagation of each C. bovis isolate in DMEM/F12 media under tissue culture conditions after 72 h. However, these results also demonstrate diminished viability of each C. bovis isolate in RPMI and DMEM after 72 h. To assess whether antibiotics could halt the growth of C. bovis under tissue culture conditions in DMEM/F12, penicillin-streptomycin (pen/strep) was added to the experimental media. This treatment was effective in eliminating all viable C. bovis in the culture system after 72 h. Our data suggest that C. bovis growth under tissue culture conditions is possible and growth in tissue culture media is nuanced. These results highlight the importance of pathogen surveillance for tumor cell lines propagated in vitro and demonstrate the need for further investigation into C. bovis growth requirements.

牛冠状杆菌在组织培养条件和培养基中的生长。
临床前癌症研究中的一个常见问题是通过癌细胞株将牛棒状杆菌引入免疫缺陷小鼠群。已知牛海绵状杆菌是在免疫缺陷小鼠间水平传递的患者来源异种移植肿瘤的污染源。然而,目前还不清楚牛肝菌是否能在哺乳动物组织培养条件或组织培养基中生长。我们假设牛海绵状芽孢杆菌不会在组织培养条件或培养基中生长,从而降低了从体外培养的肿瘤细胞系中传播的风险。我们用 CUAMC1、HAC 和 ATCC-7715 这三种牛海绵状芽孢杆菌分离物在 3 种最常用的培养人类肿瘤细胞系的培养基(包括 RPMI 1640 + 10% FBS (RPMI)、DMEM/高葡萄糖 + 10% FBS (DMEM) 和 DMEM/F-12 + 10% FBS (DMEM/F12))中测试了我们的假设。为了评估抗生素是否能阻止牛杆菌在 DMEM/F12 组织培养条件下的生长,我们在实验培养基中添加了青霉素-链霉素(pen/strep)。我们的数据表明,牛海绵状芽孢杆菌在组织培养条件下是可以生长的,而且在组织培养基中的生长是有细微差别的。这些结果突显了对体外繁殖的肿瘤细胞系进行病原体监控的重要性,并表明有必要进一步研究牛肝菌的生长要求。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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