Journal of medical entomology最新文献_第6页

Dead deer do tell tales: infestation of road-killed white-tailed deer by juvenile Ixodes scapularis (Ixodida: Ixodidae) ticks. 死鹿确实能说明问题：被公路撞死的白尾鹿会受到幼年蜱虫（蜱虫目：蜱虫科）的侵扰。

IF 2

Journal of medical entomology Pub Date : 2025-09-01 DOI: 10.1093/jme/tjaf094

William J Landesman, Abigail C Serra, Brian F Allan

{"title":"Dead deer do tell tales: infestation of road-killed white-tailed deer by juvenile Ixodes scapularis (Ixodida: Ixodidae) ticks.","authors":"William J Landesman, Abigail C Serra, Brian F Allan","doi":"10.1093/jme/tjaf094","DOIUrl":"10.1093/jme/tjaf094","url":null,"abstract":"The white-tailed deer (Odocoileus virginianus) contributes indirectly to the enzootic circulation of the Lyme disease pathogen, Borrelia burgdorferi by serving as the primary reproductive host for adult-stage blacklegged ticks (Ixodes scapularis). The role of white-tailed deer as a host for juvenile life stages is less well understood, in part because their periods of activity typically fall outside of most white-tailed deer hunting seasons. We inspected 22 road-killed deer for all stages of blacklegged ticks in Rutland County, Vermont from May to August in 2020, 2021, and 2024. Adult-stage blacklegged ticks were found attached to ten deer in May and early June. Larval-stage ticks, including ones that were partially engorged, were found on 3/5 deer inspected by hanging the head, hide, and legs over water for approximately 24 hours. We directly observed 7 nymphal-stage ticks attached to one additional deer. This study adds to a growing body of evidence that the role of juvenile feeding on white-tailed deer may be underestimated and demonstrates that the study of road-killed deer may improve our understanding of how populations of blacklegged ticks are maintained among wildlife communities.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"1372-1376"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12351483/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144621617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular identification of Anopheles moghulensis (Diptera; Culicidae) in Iran: overcoming morphological misidentification. 伊朗莫古尔按蚊（双翅目；库蚊科）分子鉴定：克服形态鉴别错误。

IF 2

Journal of medical entomology Pub Date : 2025-09-01 DOI: 10.1093/jme/tjaf095

Mehdi Badakhshan, Shamal Abdullah Al-Muffti, Leila Poorkhalil Rashid, Noorhalim Zahid Safi, Navid Dinparast Djadid, Saber Gholizadeh

{"title":"Molecular identification of Anopheles moghulensis (Diptera; Culicidae) in Iran: overcoming morphological misidentification.","authors":"Mehdi Badakhshan, Shamal Abdullah Al-Muffti, Leila Poorkhalil Rashid, Noorhalim Zahid Safi, Navid Dinparast Djadid, Saber Gholizadeh","doi":"10.1093/jme/tjaf095","DOIUrl":"10.1093/jme/tjaf095","url":null,"abstract":"Phylogenetic relationships within Anopheles species are critical for accurate classification and vector surveillance. Anopheles superpictus typically serves as a secondary vector across its distribution range and has been morphologically recognized as a single species, despite differences observed in larval and adult female characteristics. However, its close similarity to Anopheles moghulensis has led to frequent misidentifications. This study introduces a molecular species diagnostic tool based on ribosomal DNA internal transcribed spacer 2 (rDNA-ITS2) sequences for differentiating An. moghulensis from An. superpictus. Using newly designed species-specific PCR primers, we identified a misclassification wherein An. moghulensis sequences were erroneously submitted as An. superpictus. Comparative ITS2 sequence analysis revealed that An. moghulensis shares 100% similarity with An. superpictus B, with distinct ITS2 size differences: 373 bp in An. moghulensis vs. 352 bp in An. superpictus. This study represents the first molecular identification of An. moghulensis and its taxonomic placement within Iranian and global Anopheles fauna. Despite advances in species identification, inconsistencies in GenBank accession records remain a significant challenge, necessitating taxonomic reassessment of Anopheles species.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"1192-1199"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mosquitoes in small urban spaces: identification of blood meals and flight distances of engorged females in the southern Great Plains of the United States. 小城市空间中的蚊子：美国南部大平原充血雌蚊的血食鉴定和飞行距离。

IF 2

Journal of medical entomology Pub Date : 2025-09-01 DOI: 10.1093/jme/tjaf105

Brandon E Henriquez, Scott R Loss, Bruce H Noden

{"title":"Mosquitoes in small urban spaces: identification of blood meals and flight distances of engorged females in the southern Great Plains of the United States.","authors":"Brandon E Henriquez, Scott R Loss, Bruce H Noden","doi":"10.1093/jme/tjaf105","DOIUrl":"10.1093/jme/tjaf105","url":null,"abstract":"Vector-borne disease transmission can only occur when host(s), vector(s), and pathogen(s) interact in a given environment. While many studies have focused on these interactions in large urban areas, there is a need for habitat-focused studies in small urban areas where human populations are often close to wildlife and livestock. The aim of the current study was to identify the bloodmeal sources of mosquitoes in a small urban area in the southern Great Plains of the United States. Using 2 trap types, bloodmeals from 12 different hosts were detected, and the most frequently detected bloodmeal hosts were white-tailed deer, cow, and horse. The known locations of livestock at each site made it possible to identify the nearest location where mosquitoes could have fed on cows, horses, and alpacas, and we demonstrated that mosquitoes could fly distances between 200 m and 1.2 km from the bloodmeal host to the resting trap location within 30 h after taking blood. This study highlights the opportunities that are available within small urban areas to discover important host-vector relationships.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"1200-1207"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507432/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effective Aedes mosquito reduction through In2Care interventions in an extreme environment. 在极端环境下通过In2Care干预措施有效减少伊蚊。

IF 2

Journal of medical entomology Pub Date : 2025-09-01 DOI: 10.1093/jme/tjaf081

Samia Elfekih, Moataz AbdulGhaffar, Yacer Gibreel, Hedi Omer, Dan Pagendam, Majed S Nassar, Essam A Tawfik, Ary A Hoffmann

{"title":"Effective Aedes mosquito reduction through In2Care interventions in an extreme environment.","authors":"Samia Elfekih, Moataz AbdulGhaffar, Yacer Gibreel, Hedi Omer, Dan Pagendam, Majed S Nassar, Essam A Tawfik, Ary A Hoffmann","doi":"10.1093/jme/tjaf081","DOIUrl":"10.1093/jme/tjaf081","url":null,"abstract":"In2Care traps combining an insect growth regulator (pyriproxyfen) with an entomopathogen (Beauveria bassiana) have been developed as an effective way of reducing mosquito populations with promising results. Here, we test the technology in Jeddah, Saudi Arabia, against Aedes aegypti mosquitoes. In2Care traps might be particularly suitable in Jeddah because harsh climatic conditions in the hot dry season may limit Aedes movement when mosquitoes likely persist in localized sites around underground car parks and other built structures. After 8 wk of deployment, the proportion of positive traps in the intervention area was gradually reduced by 39% in the unfavorable season compared with a control area. No adults emerged from In2Care traps. Breeding containers placed in the trial site, 3 wk after termination of the trial, showed a range of emergence values from 0 to 100% compared with controls where emergence was always >80%. In subsequent interventions in three other sites, we obtained reductions of 30% to 52% based on positive traps and egg numbers per trap. However, the intervention did not reduce numbers at one site which was expected to be a mosquito hotspot and subsequently shown to have an estimated mosquito population 5.2× larger than the adjacent control area. The clear reduction at some sites and an increasing reduction in numbers over time at others, several weeks after post-In2Care monitoring operations were completed, highlight the potential of the In2Care approach in this environment. The approach could help to reduce routine pesticide-based fogging that forms the mainstay of current control measures in Jeddah.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"1243-1252"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144546666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resistance to organophosphate and pyrethroid and their underlying mechanism in Aedes albopictus (Diptera: Culicidae) from dengue endemic sub-Himalayan West Bengal, India. 印度西孟加拉邦亚喜马拉雅地区登革热流行地区白纹伊蚊对有机磷和拟除虫菊酯的抗性及其机制

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Journal of medical entomology Pub Date : 2025-09-01 DOI: 10.1093/jme/tjaf083

Subhajit Das, Prapti Das, Abhirup Saha, Subhajit Ghosh, Subarna Thakur, Dhiraj Saha

{"title":"Resistance to organophosphate and pyrethroid and their underlying mechanism in Aedes albopictus (Diptera: Culicidae) from dengue endemic sub-Himalayan West Bengal, India.","authors":"Subhajit Das, Prapti Das, Abhirup Saha, Subhajit Ghosh, Subarna Thakur, Dhiraj Saha","doi":"10.1093/jme/tjaf083","DOIUrl":"10.1093/jme/tjaf083","url":null,"abstract":"Dengue cases are increasing every year in the sub-Himalayan part of West Bengal, where Aedes albopictus (Skuse 1894) has emerged as a predominant dengue vector. Dengue management heavily relies on the recurrent application of chemical insecticides such as temephos (larvicide) and deltamethrin (adulticide). However Ae. albopictus can quickly develop resistance which complicates their management. Thus, detailed population-specific studies on resistance status and mechanism of resistance are useful for designing an effective dengue management strategy. In this study, wild mosquitoes were collected and bioassays with temephos and deltamethrin, including synergist assays, were conducted to investigate the probable mechanism of resistance. Furthermore, carboxylesterase activity and level of monooxygenase were measured followed by real-time polymerase chain reaction analysis to assess expression patterns of CCE and CYP genes. Bioassays confirmed temephos and deltamethrin resistance in Ae. albopictus population is driven by metabolic detoxification. Biochemical assay recorded significantly elevated carboxylesterase activity and monooxygenase levels compared to a susceptible laboratory strain. This is further supported by the elevated relative expression of CCE and CYP genes with consistent expression of CYP6A8 and CCEae3a in these populations. A molecular docking study suggested that the conformation of the binding site for temephos and deltamethrin may enhance their potential metabolism. These findings align with the global trend of insecticide resistance development and highlight the urgent need for more sustainable and informed approaches to dengue vector management.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"1265-1276"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144593271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cutaneous leishmaniasis in a hyperendemic metropolitan area in Iran: spatial probability modeling by machine-learning. 伊朗高流行都市地区的皮肤利什曼病：机器学习的空间概率模型。

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Journal of medical entomology Pub Date : 2025-09-01 DOI: 10.1093/jme/tjaf073

Alireza Mohammadi, Elahe Pishgar, Robert Bergquist

{"title":"Cutaneous leishmaniasis in a hyperendemic metropolitan area in Iran: spatial probability modeling by machine-learning.","authors":"Alireza Mohammadi, Elahe Pishgar, Robert Bergquist","doi":"10.1093/jme/tjaf073","DOIUrl":"10.1093/jme/tjaf073","url":null,"abstract":"Cutaneous leishmaniasis (CL), a parasitic, vector-borne infection with a broad, global distribution is prevalent in Mashhad, a city in north-eastern Iran, which is known as a significant hyperendemic area both for anthroponotic and zoonotic CL. This study evaluates the spatial distribution probability of CL prevalence by examining various factors such as socio-demography, built environment, geology, and climate within Mashhad. Data from 3,033 CL patients diagnosed between 2013 and 2020 were analyzed. Sociological, environmental, and climatic variables were assessed using the generalized linear regression model (GLM) and the maximum entropy (MaxEnt) model. The results indicate socio-demography and built environment and geology as significant influencers of the CL distribution in Mashhad. The MaxEnt model identified 42.6% (138.5 km2) of the study area as high-risk for CL, including both urban and rural areas characterized by specific geological and geographical conditions, high urbanization rates, and poor environmental quality. As confirmed by previous studies, we found that ~0.04% of the city's population were infected, young individuals with low literacy levels and those living in densely populated areas. In addition to the known environmental variables, such as temperatures between 20 and 40 °C and humidity, we also found risk areas associated with built environment and a certain type of exposed rocks that is attractive for the vector. The findings presented provide valuable insights for urban planners and health managers to target CL control programs and allocate resources effectively.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"1087-1100"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ForumA systematic review of the associations between mosquito- and tick-borne diseases and vector research in the United States. 论坛对美国蚊媒和蜱媒疾病与媒介研究之间关系的系统回顾。

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Journal of medical entomology Pub Date : 2025-09-01 DOI: 10.1093/jme/tjaf100

Yuexun Tian, Lauren DeWitt, Ryan S Almeida, Melinda Clark, Daniel Obregon, Sophie Adame, Taylor Davis, Nicole E Mendez-Maio, Gabriel L Hamer

{"title":"ForumA systematic review of the associations between mosquito- and tick-borne diseases and vector research in the United States.","authors":"Yuexun Tian, Lauren DeWitt, Ryan S Almeida, Melinda Clark, Daniel Obregon, Sophie Adame, Taylor Davis, Nicole E Mendez-Maio, Gabriel L Hamer","doi":"10.1093/jme/tjaf100","DOIUrl":"10.1093/jme/tjaf100","url":null,"abstract":"Tick- and mosquito-borne diseases in the United States are occurring at increasing rates and are heterogeneously distributed among the states. The allocation of public health resources and the attention of a research community on ticks and mosquitoes should be proportional to the number of reported human disease cases in each state. We conducted a systematic literature review of all publications on field-based studies of mosquitoes and ticks as a proxy for resource availability and research attention and compared these to the number of human tick- and mosquito-borne disease cases. The results showed that although some states have proportional publications and human disease, many deviate. This study highlights many states that have low numbers of publications on ticks or mosquitoes yet high incidence of human disease and other states that have high number of publications on ticks or mosquitoes yet a low disease incidence. This study may help public health agencies and the research community prioritize the need for increased research attention in states with the greatest disease burden.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"1047-1056"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vector Control, Pest Management, Resistance, RepellentsEvaluation of fruit sjuice preferences by Aedes, Anopheles, and Culex mosquito (Diptera: Culicidae) species for the development of effective attractive toxic sugar baits. 媒介控制、害虫管理、抗性、驱避蚊伊蚊、按蚊、库蚊对果汁的偏好研究，为研制有效、有吸引力的糖毒饵提供依据。

IF 2

Journal of medical entomology Pub Date : 2025-09-01 DOI: 10.1093/jme/tjaf099

Gaurav Kumar, Muhammad Farooq, Chander Prakash Yadav, Kai Blore, Joseph Diclaro, Himmat Singh, Whitney Qualls, Rui-De Xue

{"title":"Vector Control, Pest Management, Resistance, RepellentsEvaluation of fruit sjuice preferences by Aedes, Anopheles, and Culex mosquito (Diptera: Culicidae) species for the development of effective attractive toxic sugar baits.","authors":"Gaurav Kumar, Muhammad Farooq, Chander Prakash Yadav, Kai Blore, Joseph Diclaro, Himmat Singh, Whitney Qualls, Rui-De Xue","doi":"10.1093/jme/tjaf099","DOIUrl":"10.1093/jme/tjaf099","url":null,"abstract":"Mosquito-borne diseases pose significant public health challenges, necessitating the development of effective and sustainable vector control strategies. This study investigated the feeding preferences of 4 mosquito species (Aedes aegypti Linnaeus, Aedes albopictus Skuse, Culex quinquefasciatus Say, and Anopheles quadrimaculatus Say) for various fruit juices using a home-made choice chamber. We also assessed the impact of combining biogents lure and boric acid into attractive toxic sugar baits to enhance mosquito attraction and mortality. Choice assays revealed species-specific and sex-specific preferences. In choice assays, Aedes (Ae.) aegypti showed a preference for sweet corn juice (18.1 ± 4.0%), while Ae. albopictus preferred pumpkin juice (19.7 ± 6.0%). Female Ae. aegypti preferred sweet corn (20.2 ± 5.8%) while males preferred honey (19.8 ± 0.8%) most, but both male (22.5 ± 7.3%) and female (19.0 ± 6.8%) Ae. albopictus preferred the pumpkin most. In contrast, Cx. quinquefasciatus favored beetroot juice (17.1 ± 3.6%), and An. quadrimaculatus preferred beetroot (20.8 ± 17.4%) and peach juices (20.1 ± 9.7%). Olfactometer assays for female Ae. aegypti showed enhanced attractiveness with the addition of 3% biogents lure to beetroot juice (61.5 ± 6.5%) compared to beetroot juice (38.9 ± 6.5%) alone. Mortality bioassays confirmed the efficacy of attractive toxic sugar baits formulations containing biogents lure, boric acid, and fruit juices, achieving 86.7% to 100% mortality in all 4 mosquito species within 48 h. While these findings underscore the potential of combining natural and synthetic attractants for mosquito control; the study's laboratory setting and lack of field validation necessitate further research to ensure ecological safety and real-world applicability.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"1298-1305"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145002366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tick infestation of birds in grasslands experiencing woody plant encroachment in the United States Great Plains. 在美国大平原经历木本植物入侵的草原上，鸟类的蜱虫侵扰。

IF 2

Journal of medical entomology Pub Date : 2025-09-01 DOI: 10.1093/jme/tjaf072

Tucker C Taylor, Jozlyn D Propst, Bruce H Noden, Scott R Loss

{"title":"Tick infestation of birds in grasslands experiencing woody plant encroachment in the United States Great Plains.","authors":"Tucker C Taylor, Jozlyn D Propst, Bruce H Noden, Scott R Loss","doi":"10.1093/jme/tjaf072","DOIUrl":"10.1093/jme/tjaf072","url":null,"abstract":"Woody plant encroachment is one of the largest threats to grasslands of the US Great Plains. Its spread, mainly due to fire suppression, affects entire ecosystems, including arthropod vectors, bird communities, and the ecology of vector-borne disease. Eastern redcedar (Juniperus virginiana), one of the primary encroaching species in this region, is known to increase abundance of pathogen-carrying tick species such as Amblyomma americanum; however, the role birds play in carrying ticks in association with eastern redcedar encroachment is unknown. In areas of Oklahoma representing 3 stages of eastern redcedar encroachment, we mist-netted birds, sampled larval and nymphal ticks from them, and evaluated tick infestation of birds from May to October 2023. Of 140 birds sampled, 25.7% were infested with ticks, a higher prevalence of infestation than in most previous studies of birds in the United States. Notably, some birds were infested with Ixodes scapularis, which has rarely been found on wildlife other than reptiles in the southern US. There were no significant differences in prevalence or intensity of tick infestation in birds across differing levels of eastern redcedar encroachment, indicating that a high proportion of birds carry ticks in all encroachment stages. This study provides the first evidence that birds contribute to the ecology of tick-borne disease systems in grasslands experiencing woody plant encroachment. Our results, which suggest birds are likely moving ticks into, out of, and among eastern redcedar-encroached grasslands of the US Great Plains, can help inform land management and public health efforts seeking to reduce disease risk.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"1306-1316"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507430/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144513019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular species delimitation analysis of Leptotrombidium spp. and other chigger species parasitizing birds in Malaysia. 马来西亚细恙螨及其他寄生鸟类恙虫的分子种划分分析。

IF 2

Journal of medical entomology Pub Date : 2025-09-01 DOI: 10.1093/jme/tjaf078

Praveena Rajasegaran, Kim-Kee Tan, Jing Jing Khoo, Mohammad Saiful Mansor, Mohd K S Ahmad Khusaini, Sazaly AbuBakar, Zubaidah Ya'cob, Benjamin L Makepeace

{"title":"Molecular species delimitation analysis of Leptotrombidium spp. and other chigger species parasitizing birds in Malaysia.","authors":"Praveena Rajasegaran, Kim-Kee Tan, Jing Jing Khoo, Mohammad Saiful Mansor, Mohd K S Ahmad Khusaini, Sazaly AbuBakar, Zubaidah Ya'cob, Benjamin L Makepeace","doi":"10.1093/jme/tjaf078","DOIUrl":"10.1093/jme/tjaf078","url":null,"abstract":"Trombiculid mites (Acariformes) are unique among arthropods of medical importance in that only the larval instar (chigger) is parasitic, which can result in the transmission of zoonotic scrub typhus. The use of molecular approaches for chigger species discrimination has been very limited until recently, especially for those parasitizing bird hosts, where data remain scarce. Here, we aimed to generate DNA barcodes of chiggers parasitizing birds in Malaysia based on the mitochondrial cytochrome c oxidase subunit I (COI) gene following DNA extraction, PCR and sequencing. Fifty-four COI sequences from 8 bird-associated chigger species in Malaysia were combined with 50 GenBank sequences comprising 7 genera from various countries for DNA barcode and phylogenetic analysis. The correct identification rates for the 95 COI barcodes were 96.84% (Best Match) and 86.31% (Best-Close Match). DNA barcode analyses effectively clustered the 8 nominal species from this study into their respective genera. Genetic divergence of less than 3% was observed within Ascoschoengastia lorius, Neoschoengastia gallinarum, Parascoschoengastia heynemani, Leptotrombidium imphalum, and Blankaartia acuscutellaris, all of which formed a monophyletic clade, confirming their conspecific nature. Conversely, intraspecific divergences of 17.64%, 15.49%, and 11.63% were obtained for Toritrombicula densipiliata, Odontacarus audyi, and Leptotrombidium deliense. These divergences, supported by evidence of distinct entities through delimitation analyses, indicate potential cryptic diversity within these populations. In conclusion, this study represents the first molecular genetic analysis of bird chiggers in Malaysia, revealing varying levels of genetic divergence. Our findings highlight the utility of DNA barcoding for understanding chigger diversity and aiding in accurate identification.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"1175-1191"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507433/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144513016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0