Journal of materials chemistry. B最新文献

{"title":"Photodegradable polyacrylamide tanglemers enable spatiotemporal control over chain lengthening in high-strength and low-hysteresis hydrogels.","authors":"Joshua S Lee, Bruce E Kirkpatrick, Abhishek P Dhand, Lea Pearl Hibbard, Benjamin R Nelson, Nathaniel P Skillin, Makayla C Johnson, Dilara Batan, Benjamin D Fairbanks, Timothy J White, Christopher N Bowman, Jason A Burdick, Kristi S Anseth","doi":"10.1039/d4tb02149e","DOIUrl":"10.1039/d4tb02149e","url":null,"abstract":"<p><p>Covalent hydrogel networks suffer from a stiffness-toughness conflict, where increased crosslinking density enhances the modulus of the material but also leads to embrittlement and diminished extensibility. Recently, strategies have been developed to form highly entangled hydrogels, colloquially referred to as tanglemers, by optimizing polymerization conditions to maximize the density and length of polymer chains and minimize the crosslinker concentration. It is challenging to assess entanglements in crosslinked networks beyond approximating their theoretical contribution to mechanical properties; thus, in this work, we synthesize and characterize polyacrylamide tanglemers using a photolabile crosslinker, which allows for direct assessment of covalent trapping of entanglements under tension. Further, this chemistry allows tuning of the modulus <i>in situ</i> by crosslink photocleavage (from tensile modulus (<i>E</i>_T) = 100 kPa to <25 kPa). Beyond cleavage of crosslinks, we demonstrate that even non-degradable tanglemer formulations can be photo-softened and completely degraded through Fe³⁺-mediated oxidation of the polyacrylamide backbone. While both photodegradation methods are useful for spatial patterning and result in softer gels with reduced fracture strength, only crosslink photocleavage improves gel extensibility <i>via</i> light-induced chain lengthening (<i>ε</i>_F = 700% to >1500%). Crosslink photocleavage in tanglemers also affords significant control over localized swelling and diffusivity. In sum, we introduce a simple and user-directed approach for probing entanglements and asserting spatiotemporal control over stress-strain responses and small molecule diffusivity in polyacrylamide tanglemers, suggesting a multitude of potential soft matter applications including controlled release and tunable bioadhesive interfaces.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626382/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142796637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nebulized inhalation drug delivery: clinical applications and advancements in research.","authors":"Ruyi Yan, Chang Zou, Xiaohang Yang, Weihua Zhuang, Yushi Huang, Xiuli Zheng, Jie Hu, Lingni Liao, Yongchao Yao, Xuping Sun, Wenchuang Walter Hu","doi":"10.1039/d4tb01938e","DOIUrl":"https://doi.org/10.1039/d4tb01938e","url":null,"abstract":"<p><p>Nebulized inhalation administration refers to the dispersion of drugs into small droplets suspended in the gas through a nebulized device, which are deposited in the respiratory tract by inhalation, to achieve the local therapeutic effect of the respiratory tract. Compared with other drug delivery methods, nebulized inhalation has the advantages of fast effect, high local drug concentration, less dosage, convenient application and less systemic adverse reactions, and has become one of the main drug delivery methods for the treatment of respiratory diseases. In this review, we first discuss the characteristics of nebulized inhalation, including its principles and influencing factors. Next, we compare the advantages and disadvantages of different types of nebulizers. Finally, we explore the clinical applications and recent research developments of nebulized inhalation therapy. By delving into these aspects, we aim to gain a deeper understanding of its pivotal role in contemporary medical treatment.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142804231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative features of osteo-bioactive Ti surfaces at the atomic/molecular level.","authors":"Fengxiong Luo, Dongxuan Li, Yu Yang, Jiajun Liu, Ruiqi Mao, Yawen Huang, Jian Lu, Xiangdong Zhu, Kefeng Wang, Yujiang Fan, Xingdong Zhang","doi":"10.1039/d4tb02195a","DOIUrl":"https://doi.org/10.1039/d4tb02195a","url":null,"abstract":"<p><p>The osteo-bioactive potential of biomaterials can be modulated by altering material properties such as chemical composition, surface topography, and geometry. The correlation between the physicochemical properties of biomaterials and their osteo-bioactivity is highly complex. As a material widely used in bone repair, the structure-activity/dose-effect relationship between titanium (Ti) surface features and osteo-bioactivity remains unclear. To quantitatively enhance the osteogenic activity of Ti, we employed femtosecond laser (FSL) technology to create Ti surfaces with gradient changes in bioactive (nucleation) sites. Based on the apatite deposition ability on the etched surfaces, the quantitative relationship between the osteo-bioactivity of Ti surfaces and their characteristic parameters was systematically explored. Concurrently, classical molecular dynamics (MD) simulations were utilized to investigate the aggregation behavior of calcium and phosphate ions on the Ti surfaces with different sites. The findings from mineralization experiments revealed that the type and density of bioactive sites could influence the deposition of apatite. It was further identified that TiO₂ and Ti-OH are the crucial bioactive sites, with basic Ti-OH exhibiting superior efficacy as a bioactive site compared to its acidic counterpart. Moreover, the bioactive (nucleation) site densities should be maintained at a minimum of 2.33 ± 0.55 nm^-2 for TiO₂ and 2.50 ± 0.59 nm^-2 for -OH, ensuring satisfactory osteo-bioactivity on the Ti surface. The results provide the atomic/molecular features of Ti surfaces that effectively foster apatite deposition and bioactivity, promoting the rapid progression of titanium-based bone repair materials.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142788216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In situ</i> fabricated gold nanostars on hydrogel beads as photo-oxidase mimics for rapid and sustainable POCT of uric acid.","authors":"Tanushree Das, Saurav Das, Debapratim Das","doi":"10.1039/d4tb02096k","DOIUrl":"https://doi.org/10.1039/d4tb02096k","url":null,"abstract":"<p><p>Synthetic enzyme mimics surpass their natural counterparts in terms of stability, efficiency, and cost-effectiveness, making them highly valuable for catalytic applications. Gold nanomaterials, particularly gold nanostars, have emerged as promising enzyme mimetic nanocatalysts due to their enhanced light interaction and superior catalytic efficiency. In this study, gold nanostars grown <i>in situ</i> on the surface of core-shell hydrogel beads exhibited specific oxidase-like activity when exposed to light. Photoexcitation of gold nanostars generates singlet oxygen through the interaction of positive holes and superoxide radicals, resulting in photo-oxidase-like activity. Attaching the gold nanostars to the hydrogel bead surface prevented catalytic activity loss caused by agglomeration, resulting in a marked improvement in catalytic stability. This stability is evident from the sustained catalytic activity of the hydrogel bead-embedded gold nanostars, even after 60 days of prolonged incubation in an aqueous medium, and their strong catalytic performance across multiple reaction cycles. Leveraging this photo-oxidase-like activity, a point-of-care testing (POCT) setup is developed for highly sensitive uric acid detection. The system achieved a remarkable detection limit of 0.9 μM and demonstrated excellent accuracy in blood serum and urine sample analyses. Furthermore, the integration of smartphone technology facilitated rapid and convenient on-site testing, bridging the gap between laboratory settings and real-world applications. This approach offers a practical and sustainable solution for efficient and accurate uric acid monitoring in diverse settings.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142788199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent progress towards the development of fluorescent probes for the detection of disease-related enzymes.","authors":"Lopamudra Mishra, Monalisa Mishra","doi":"10.1039/d4tb01960a","DOIUrl":"10.1039/d4tb01960a","url":null,"abstract":"<p><p>Normal physiological functions as well as regulatory mechanisms for various pathological conditions depend on the activity of enzymes. Thus, determining the <i>in vivo</i> activity of enzymes is crucial for monitoring the physiological metabolism and diagnosis of diseases. Traditional enzyme detection methods are inefficient for <i>in vivo</i> detection, which have different limitations, such as high cost, laborious, and inevitable invasive procedures, low spatio-temporal resolution, weak anti-interference ability, and restricted scope of application. Because of its non-destructive nature, ultra-environmental sensitivity, and high spatiotemporal resolution, fluorescence imaging technology has emerged as a potent tool for the real-time visualization of live cells, thereby imaging the motility of proteins and intracellular signalling networks in tissues and cells and evaluating the binding and attraction of molecules. In the last few years, significant advancements have been achieved in detecting and imaging enzymes in biological systems. In this regard, the high sensitivity and unparalleled spatiotemporal resolution of fluorescent probes in association with confocal microscopy have garnered significant interest. In this review, we focus on providing a concise summary of the latest developments in the design of fluorogenic probes used for monitoring disease-associated enzymes and their application in biological imaging. We anticipate that this study will attract considerable attention among researchers in the relevant field, encouraging them to pursue advances in the development and application of fluorescent probes for the real-time monitoring of enzyme activity in live cells and <i>in vivo</i> models while ensuring excellent biocompatibility.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142788217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PEGylation of indium phosphide quantum dots prevents quantum dot mediated platelet activation.","authors":"Leigh Naylor-Adamson, Thomas W Price, Zoe Booth, Sophie V L Leonard, Juan Gallo, Le Duc Tung, Stanley Harvell-Smith, Nguyen Thi Kim Thanh, Zabeada Aslam, David Allsup, Nicole Hondow, Thomas Chamberlain, Jürgen E Schneider, Khalid Naseem, Jean-Sebastien G Bouillard, Graeme J Stasiuk, Simon D J Calaminus","doi":"10.1039/d4tb01334d","DOIUrl":"10.1039/d4tb01334d","url":null,"abstract":"<p><p>Quantum dots (QDs) are semiconducting inorganic nanocrystals, that have garnered interest in biological and medical spheres due, to their potential benefits in biomedical imaging and drug-delivery systems. Indium phosphide QDs shelled with zinc sulphide (InP/ZnS) are viewed as more biocompatible than previous heavy metal based QDs. However, little is known about how InP/ZnS QDs affect a key blood cell, the platelet. Understanding how platelets interact with QDs is critical as unwanted activation can lead to pathological thrombus formation. Herein, we demonstrate PEGylation of InP/ZnS QDs coated with lipoic acid (QD-LA) or coated with penicillamine (QD-Pen) surface ligands induced markedly less platelet aggregation, platelet-QD interactions, integrin activation, alpha granule secretion and restored platelet spreading in washed platelets in comparison to their non-PEGylated counterparts. Furthermore, in whole blood, PEGylation of QDs reduced the number of QDs in the thrombus, thereby helping to minimise the chance of dysfunctional thrombus formation. Overall, we show that QD PEGylation is important to help prevent QD mediated platelet activation. In combination with the most biocompatible coating, PEGylation markedly reduced platelet activation, widening the concentrations at which QDs were viable for development as potential drug delivery or imaging agents.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11619005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142788214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emissive triphenylamine functionalised 1,8-naphthalimide and naphthalene diimide fluorophores: aggregation, computation and biological studies.","authors":"Laura Ramírez Lázaro, L Constance Sigurvinsson, Niamh Curtin, Joanna Ho, Ena T Luis, Deirdre A McAdams, Tómas A Gudmundsson, Chris S Hawes, Denis Jacquemin, Donal F O'Shea, Eoin M Scanlan, Thorfinnur Gunnlaugsson, Adam F Henwood","doi":"10.1039/d4tb01905a","DOIUrl":"https://doi.org/10.1039/d4tb01905a","url":null,"abstract":"<p><p>Four new aromatic imides bearing triphenylamino (TPA) moieties are reported each of which differ by the number and/or positional arrangements of the TPA units. Compounds 1-3 are 1,8-naphthalimides (naps) that contain <i>N</i>,<i>N</i>'-diphenyl-[1,1'-biphenyl]-4-amino (TPA-Ph) groups appended to the <i>N</i>-termini of the respective imides. Each differs by their functionalisation of the 4-position of the nap: nitro (1), amino (2), or an additional TPA group (3). By contrast, compound 4 is a naphthalene diimide (NDI) functionalised with TPA-Ph moieties on each <i>N</i>-terminus. These simple modifications produce molecules with vastly different optoelectronic and aggregation properties. This article studies these characteristics with particular focus directed toward the contrast in aggregation-caused quenching (ACQ) properties of 2 compared with the aggregation-induced emission (AIE) properties of 3. The distinct aggregation and photophysical properties of 2 and 3 are delicately exploited using self-assembly with an amphiphilic poloxamer to generate nanoparticles capable of delivering 2 and 3 into cells for biological imaging.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142788201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leveraging nanomaterials for ultrasensitive biosensors in early cancer detection: a review.","authors":"Drishti Khandelwal, Aheli Bhattacharya, Vanshika Kumari, Sachchidanand Soaham Gupta, Kumar Rakesh Ranjan, Vivek Mishra","doi":"10.1039/d4tb02107j","DOIUrl":"https://doi.org/10.1039/d4tb02107j","url":null,"abstract":"<p><p>Cancer remains a major global health challenge with a high mortality rate, as evidenced by the rise in new cases every year. Conventional diagnostic methods like PET scans, MRIs, and biopsies, despite being widely used, suffer from significant drawbacks such as high radiation exposure, difficulty in distinguishing malignant from benign tumors, and invasiveness. Early detection, which is crucial for improving treatment outcomes and survival rates, is hindered by the asymptomatic nature of early-stage cancer and the limitations of current diagnostic tools. Cancer biomarkers, detectable in body fluids, offer valuable diagnostic information, and recent advances in nanotechnology have led to the development of highly sensitive nano-biosensors. This review explores recent advancements (2022-2024) in the field of ultrasensitive nano-biosensors, emphasizing the strategic integration of nanomaterials to enhance sensitivity and accuracy in cancer biomarker detection. It highlights how precise nanomaterial positioning in sensor components like electrodes and bioreceptors enables early cancer diagnosis at low biomarker concentrations. These innovations underscore the transformative potential of nanomaterials in revolutionizing early cancer diagnostics, improving patient care, and enhancing survival outcomes.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142782306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A controlled co-assembly approach to tune temperature responsiveness of biomimetic proteins.","authors":"Nisal Wanasingha, Rajkamal Balu, Agata Rekas, Jitendra P Mata, Naba K Dutta, Namita Roy Choudhury","doi":"10.1039/d4tb01737d","DOIUrl":"https://doi.org/10.1039/d4tb01737d","url":null,"abstract":"<p><p>The controlled co-assembly of biomacromolecules through tuneable interactions offers a simple and fascinating opportunity to assemble multiple molecules into a single entity with enhanced complexity and unique properties. Herein, our study presents a dynamic co-assembled system using the multistimuli responsive intrinsically disordered protein Rec1-resilin and the adhesive hydrophilic protein silk sericin (SS). We utilized advanced characterization techniques including circular dichroism (CD) spectroscopy, dynamic light scattering (DLS), small-angle X-ray scattering (SAXS), and small/ultra-small angle neutron scattering (SANS/USANS) to elucidate the detailed co-assembly behavior of the system and its evolution over time and temperature. To achieve sufficient neutron contrast, we successfully biosynthesised deuterium-labeled Rec1-resilin (D-Rec1). Our research demonstrates that this co-assembly allows the formation of a robust entity with dynamic conformational assembly and disassembly, exhibiting both the upper critical solution temperature (UCST) and lower critical solution temperature (LCST) with reversibility. The assembly and disassembly dynamics of the co-assembled entity at UCST are very fast, while the process is kinetically controlled at LCST. This study provides significant new insights into the interplay of a hydrophilic, multi-responsive IDP and a highly hydrophilic protein, shaping the thermoresponsive and stable properties of the co-assembled entity.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142775897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Topical siRNA therapy of diabetic-like wound healing.","authors":"Eva Neuhoferova, Marek Kindermann, Matej Buzgo, Karolina Vocetkova, Dalibor Panek, Petr Cigler, Veronika Benson","doi":"10.1039/d4tb01547a","DOIUrl":"https://doi.org/10.1039/d4tb01547a","url":null,"abstract":"<p><p>Non-healing wounds are a serious complication in diabetic patients. One of the detrimental factors contributing to limited wound healing is the accumulation of metalloproteinase-9 (MMP-9) in the wound. Selective inhibition of MMP-9 is one of the established therapeutic targets for diabetic wound healing. Here, a functional and biocompatible wound dressing is developed to enable a controlled release of a traceable vector loaded with the antisense siRNA against MMP-9 in the wound. The dressing consists of degradable polymer nanofibers embedded with a vector nanosystem - polymer-coated fluorescent nanodiamonds optimized for the binding of siRNA and colloidal stability of nanodiamond-siRNA complexes in a physiological environment. The developed dressing is tested on murine fibroblasts and also applied to wounds in a diabetic murine model to evaluate its suitability in terms of <i>in vivo</i> toxicity, biological efficacy, and handling. The treatment results in significant local inhibition of MMP-9 and a shortening of the wound healing time. The scar formation in treated diabetic-like mice becomes comparable with that in non-treated diabetes-free mice. Our results suggest that the application of our biocompatible dressing loaded with a non-toxic vector nanosystem is an effective and promising approach to gene therapy of non-healing wounds.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142775210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}