Journal of materials chemistry. B最新文献

{"title":"An intelligent ZIF-based nanoplatform for photothermal/chemodynamic-induced combination therapy with O₂ evolution properties for improved infected wound regeneration.","authors":"Rezvaneh Azizi, Mehraneh Kermanian, Vajihe Alinezhad, Ali Kalantari-Hesari, Satar Yousefiasl, Lidia Maeso, Gorka Orive, Abbas Mohammadi, Kimia Esmaeilzadeh, Mohammad Seyedhamzeh, Faezeh Almasi, Aziz Maleki","doi":"10.1039/d5tb00934k","DOIUrl":"https://doi.org/10.1039/d5tb00934k","url":null,"abstract":"<p><p>The bacteria and the damage caused by reactive oxygen species (ROS) significantly contribute to the delayed healing of infected skin wounds. Photothermal therapy (PTT), as a promising antibacterial platform, has the ability to reduce bacterial colonization and infection, eliminate ROS, alleviate inflammation, and expedite the process of wound healing. In this study, a multifunctional ZIF-based nanosystem was developed in a facile and one-pot manner, leveraging the unique properties of Cu ions, zeolitic imidazolate framework-8 (ZIF-8), and β-sitosterol (BSTL) (abbreviated as BSTL-O₂-Cu-ZIF) to provide a comprehensive solution for wound healing. The nanosystem exhibited excellent nanozyme activity, resulting in potent antibacterial effects beneficial for wound healing. Furthermore, our findings confirmed that the BSTL-O₂-Cu-ZIF nanosystem can release oxygen <i>via</i> Fenton-like reactions, enhancing the efficiency of wound healing. Moreover, the Cu-doped nanoplatforms displayed effective photothermal capacity, which not only endowed the nanostructures with antibacterial properties but also positively influenced the healing process, as confirmed by histomorphological examinations. These results indicate that the BSTL-O₂-Cu-ZIF nanosystem can efficiently promote skin incision closure and accelerate infected wound healing, making it a promising solution for wound care.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144577451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Galactosylated silver nanoparticles as a biocompatible intrinsic SERS probe for bladder cancer imaging and <i>ex vivo</i> tumor detection.","authors":"Ting-Yu Cheng, Yi-Chun Chiu, Kuan-Hsu Chen, Ya-Jyun Chen, Chih-Chia Huang","doi":"10.1039/d5tb00546a","DOIUrl":"https://doi.org/10.1039/d5tb00546a","url":null,"abstract":"<p><p>The biological application of silver nanoparticles (Ag NPs), which are commonly used as SERS substrates, is often limited by issues related to uncontrolled Ag ion release, resulting in instability and cytotoxicity. In this study, we developed galactosylated Ag@PGlyco-PSMA NPs, a novel biocompatible and bio-SERS platform for sensing small molecules at nanomolar concentration levels, achieving an analytical enhancement factor of 1.71 × 10⁴ alongside intrinsic imaging and labeling capabilities for bladder cancer cells. These Ag NPs were co-synthesized during the polymerization of <i>o</i>-nitrophenyl-β-D-galactopyranoside to form an Ag@polyaniline-based glycopolymer (PGlyco) nanostructure, which was subsequently reacted with poly(styrene-<i>alt</i>-maleic acid) (PSMA). This process stabilized the particle dispersion while generating robust SERS signals due to PGlyco immobilization. By controlling the formation kinetics through the addition of the PSMA polymer at 30 seconds after the reaction of Ag@PGlyco NPs, we observed the formation of aggregate-induced hot spots to evolve PGlyco-related SERS signals arising from interparticle interactions. Our results demonstrated that Ag@PGlyco-PSMA NPs exhibit minimal Ag ion release, resulting in over 80% cell viability across T24, MB49, VERO, and SV-HUC-1 cell lines. Among these cells, Ag@PGlyco-PSMA nanoparticles demonstrated remarkable capability for enhancing cellular uptake, effectively distinguishing bladder cancer cells from normal cells with over 2.6 folds of the signal difference in SERS imaging. The galactose moieties in the PGlyco coating around the Ag@PGlyco-PSMA nanoparticles served as a SERS probe for multivalent binding to bladder cancer cells, enabling cancer imaging diagnosis and tumor-specific detection in accordance with tumor volume growth. Our findings indicated that Ag@PGlyco-PSMA nanoparticles offered intrinsic SERS capability for galactose-mediated bio-interaction and minimal Ag ion release, showing an ideal diagnostic optical platform for <i>in vitro</i> cancer cell imaging and <i>ex vivo</i> tumor progression tracking through bladder SERS detection.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144577453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into advanced dressings based on shape memory materials for wound management.","authors":"Tianran Song, Kun Li, Jingxi Wang, Xuemei Sun, Shuang Li, Chaojuan Yang, Ping Li","doi":"10.1039/d5tb00695c","DOIUrl":"https://doi.org/10.1039/d5tb00695c","url":null,"abstract":"<p><p>Wound is the breakage of the skin caused by external damage, and it greatly enhances the risk of infection. Severe chronic wounds can typically lead to the deterioration of the wound condition, prolonged wound healing, complex medical treatment and even mortality. As a reliable strategy, diverse innovative medical dressings have demonstrated significant potential in accelerating wound healing. Shape memory materials can respond to specific external stimuli, such as heat, light, or moisture, to revert to their original shapes and possess good biocompatibility, adaptability and tunable biodegradability, which have exhibited quite valuable applications in biomedical fields such as cardiovascular stents and self-tightening sutures. In this review, the routine wound healing process and common wound dressings including electrospun fibers, hydrogels and sponges are first summarized. Then, the classification and different activation mechanisms of shape memory materials are analyzed, with a focus on comparison among thermally, optically, and water-driven modes, highlighting the potential of water-driven modes in biomedical applications. Finally, the latest research progress on shape memory materials for wound healing, including self-contractive dressing, drug-delivery dressing and hemostatic dressing, are systematically reviewed and highlighted. Finally, the current challenges and prospects are analyzed, with the aim to provide a preliminary discussion and scientific reference for building more effective wound treatment dressings.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144577454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From simple delivery to multimodal systems: the critical role of macromolecular platforms in bioorthogonal drug synthesis.","authors":"Tieze van den Elsen, Kevin Neumann","doi":"10.1039/d5tb01111f","DOIUrl":"https://doi.org/10.1039/d5tb01111f","url":null,"abstract":"<p><p>Targeted drug delivery strategies have emerged as promising solutions to overcome traditional challenges such as poor bioavailability and side effects associated with conventional drug delivery methods. Among these strategies, the use of prodrugs offers a viable approach by leveraging enzymatic or controlled chemical transformations <i>in vivo</i> to enhance drug efficacy and specificity. The advent of bioorthogonal chemistry has revolutionized prodrug activation, providing a multitude of activation strategies beyond conventional methods. This review explores the integration of bioorthogonal chemistry, particularly transition metal catalysis, into prodrug activation strategies, with a focus on the use of macromolecular scaffolds as platforms to enable and localize these chemistries in biological environments. Specifically, this review focuses on the growing field of <i>in situ</i> bond-forming synthesis mediated by transition metal catalysts, often enabled by the use of macromolecular platforms. By forming carbon-carbon or carbon-heteroatom bonds intra- or intermolecularly, this approach offers advantages over traditional uncaging strategies through the absence of the pharmacoactive motif in the prodrug. We emphasize the central role of macromolecular platforms in integrating bioorthogonal chemistries into multimodal systems that enable targeting strategies and stimuli-responsive behavior, both crucial for achieving site-specific activation and minimizing off-target effects. We conclude that the future of this field lies with the development of retrosynthetic prodrug design, and the use and development of multimodular macromolecular platforms to host and enable new bioorthogonal transition metal catalysis.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144577452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-functionalized graphene oxide induces M2a and M2c macrophage polarization to orchestrate inflammation and tissue remodeling.","authors":"Hui Li, Hefeng Yang, Bo Ma, Jia Qiao, Fanfan Chen, Pinwen Wang, Riyue Yu, Jingjing Sun, Yuanwei Chen","doi":"10.1039/d5tb00474h","DOIUrl":"https://doi.org/10.1039/d5tb00474h","url":null,"abstract":"<p><p>Functional tissue repair is often constrained by inflammation and fibrosis. Alternatively activated M2 macrophages have emerged as promising therapeutic targets for optimizing graft-to-host interactions; however, efficient induction methods are required. Presumably, the outcome of regenerative wound healing or scar formation/fibrosis might be dependent on the balance between M2a and M2c sub-phenotypes. This study utilized dual-functionalized graphene oxide (GO) as a DNA delivery agent to induce M2a and M2c macrophage polarization. Mechanistically, molecular characteristics were analyzed using RNA sequencing. We designed GO with polyethyleneimine (PEI) modification and subsequently conjugated it with polyethylene glycol (PEG)-folate (FA) to target human THP-1-derived macrophage activation. Specifically, the resulting GO-PEI-PEG-FA (GPPF) compound effectively activated CD206⁺CD209⁺M2a and CD163⁺MerTK⁺M2c phenotype polarization. The efficient delivery of IL4 or IL10 plasmid DNA using GPPF (GPPF/pIL4 or GPPF/pIL10) significantly enhanced macrophage cellular elongation and reduced MHC-II-associated antigen presentation. M2a(GPPF/pIL4) and M2c(GPPF/pIL10) were validated as negative regulators of the immune response and positive regulators of Th2 effectors. Up-regulated genes in M2a(GPPF/pIL4) even inhibited type I interferon production and restricted the innate immune response. Supplemental to the established data, M2a(GPPF/pIL4) behaved similar to IFN-responsive macrophages, restricting viral life cycles and promoting myogenesis and osteogenesis. Meanwhile, M2c(GPPF/pIL10) was characterized using IL10 signaling, anti-fibrosis, and neutrophil-mediated suppression of the LPS-bacterial response. Regarding the tissue remodeling process, the two subsets attenuated negative-regulated BMP signaling to facilitate osteoinduction and up-regulated NAMPT to establish a transient stem cell-activating niche for tissue regeneration. This study underscored the potential of functionalized GO-induced M2 sub-phenotypes as modulators in regenerative medicine.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional nanodrug-enabled mild photothermal therapy for enhanced immunotherapy in triple-negative breast cancer.","authors":"Xinyue Du, Ying Zhang, Bingxin Gu, Ziyi Yang, Xiaoping Xu, Haibao Peng, Shaoli Song, Zhongyi Yang","doi":"10.1039/d5tb00322a","DOIUrl":"https://doi.org/10.1039/d5tb00322a","url":null,"abstract":"<p><p>Triple-negative breast cancer (TNBC) responds poorly to immune checkpoint blockade (ICB) based immunotherapy owing to the lack of immunogenicity as well as a highly immunosuppressive tumor microenvironment (TME). Mild photothermal therapy (PTT) can induce an immune-favorable tumor microenvironment and has been proposed to sensitize tumors to ICB therapy. Drawing from this concept, a novel multifunctional nanodrug, ICG@Tf-DTPA-Gd, was developed. This nanodrug, which combines transferrin (Tf) with Gd-DTPA and ICG, is designed for multimodal imaging-guided mild PTT, aiming to enhance immunogenicity and facilitate effective ICB immunotherapy in TNBC. When exposed to an 808 nm laser, the ICG@Tf-DTPA-Gd NPs exhibited exceptional photothermal performance, effectively triggered immunogenic cell death (ICD) and promoted the maturation of dendritic cells (DCs) <i>in vitro</i>. <i>In vivo</i> studies revealed strong multimodal imaging capabilities, consisting of magnetic resonance imaging (MRI), fluorescence imaging (FLI), and photothermal imaging. When used in combination with anti-programmed death-ligand 1 (PD-L1), this photo-immunotherapy system induced ICD and DC maturation, and increased the infiltration of cytotoxic T lymphocytes (CTLs) into tumors, which resulted in significant reduction in primary tumor foci and pulmonary metastases. As a result, this approach not only triggers a robust adaptive immune response but also transforms the immunologically \"cold\" TNBC into a \"hot\" tumor, enabling effective anti-PD-L1 therapy. This provides a promising alternative and valuable reference for effective immunotherapy of TNBC.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pt(IV)-functionalised polyacrylic acid-coated iron oxide magnetic nanoparticles as redox-responsive cancer theranostics.","authors":"Beatriz Brito, Thomas W Price, Cátia V Rocha, Manuel Bañobre-López, Graeme J Stasiuk, Juan Gallo","doi":"10.1039/d5tb01007a","DOIUrl":"10.1039/d5tb01007a","url":null,"abstract":"<p><p>Iron oxide nanoparticles represent a class of nanomaterials with unique physicochemical properties and high potential for theranostic applications. Herein, we functionalised polyacrylic acid (PAA)-coated iron oxide nanoparticles with a chemotherapeutic Pt(IV) prodrug, to prepare Fe₃O₄@PAA-Pt(IV) nanostructures that act as <i>T</i>₂ MR theranostics with redox- (and thus TME-) responsive therapeutic properties. The synthesis of Fe₃O₄@PAA-Pt(IV) nanoparticles was optimised to yield nanoparticles with appropriate hydrodynamic diameter and Pt/Fe ratio. The Fe₃O₄@PAA-Pt(IV) nanoparticles displayed promising magnetic and relaxometric properties, showing a higher relaxivity than commercially available NP-based MRI agent Resovist®. Cell internalisation studies in 2D and 3D cell models demonstrated that the nanomaterials accumulated in cancer cells after only 6 h of incubation at a concentration that allowed for contrast enhancement in MRI. Cell viability studies showed that Fe₃O₄@PAA-Pt(IV) nanoparticles were 2.5 times more effective than the Pt(IV) prodrug in inducing apoptosis (IC₅₀ = 156 μM <i>vs.</i> 379 μM) in 2D models, while in 3D models, they were found to be as effective as active drug cisplatin. These results show the potential of these versatile Pt(IV)-functionalised PAA-coated iron oxide nanostructures as redox responsive MR theranostics for cancer therapy.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12227113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144565582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Addressing the polycation dilemma in drug delivery: charge-converting liposomes.","authors":"Martyna Truszkowska, Ahmad Saleh, Melanie Lena Ebert, Gergely Kali, Andreas Bernkop-Schnürch","doi":"10.1039/d5tb00945f","DOIUrl":"https://doi.org/10.1039/d5tb00945f","url":null,"abstract":"<p><p>The aim of this study was to tackle the polycation dilemma in drug delivery by developing charge-converting liposomes capable of permeating the mucus gel layer and enhancing cellular uptake. Positively charged liposomes containing dioleoylphosphatidylethanolamine (DOPE), cholesterol, and oleyl-oligolysine were formulated <i>via</i> the thin-film method. These liposomes were coated with polyphosphate to create negatively charged phosphorylated liposomes (pp-liposomes). Liposomes were characterized regarding droplet size, zeta potential, stability, cytotoxicity, and hemolytic activity. The cleavage of polyphosphates from the surface of liposomes triggered by intestinal alkaline phosphatase (AP) was monitored <i>via</i> malachite green assay and shift in zeta potential. Mucus permeation was assessed using porcine intestinal mucus in Transwell inserts. Cellular uptake was quantified in Caco-2 cells by flow cytometry and confocal microscopy. Liposomes exhibited an average size of 138.7 ± 2.9 nm and a zeta potential of +35.4 ± 1.5 mV, while the size of polyphosphate-coated liposomes increased to 168.4 ± 1.2 nm with a zeta potential of -24.2 ± 2.5 mV; both remained stable over 24 hours. Liposomes were non-toxic and hemolytic in a concentration of 0.1%. pp-Liposomes were less toxic than uncoated liposomes. Significant phosphate release occurred within first 6 hours of incubation with AP, and the zeta potential converted to +12.9 ± 5.19 mV within 24 hours. Mucus permeation studies showed that pp-liposomes exhibited 12-fold increase in permeability in the absence of AP compared to its presence. Cellular uptake of liposomes and pp-liposomes in Caco-2 cells demonstrated comparable levels of internalization. Accordingly, charge-converting liposomes effectively traversed the mucus barrier and improved cellular uptake, indicating a promising approach to resolving the polycation dilemma.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyphosphate coacervate gels for manufacturing of manganese loaded glass powders and fibres: structural, cytocompatibility and surface bioactivity study.","authors":"Chiara Cavazzoli, Roberto Di Pasquale, Zarrin Moghaddam, Hongjuan Zhao, Agron Hoxha, Lauren Lewendon, Monica Felipe-Sotelo, Carol Crean, Alfonso Zambon, Gigliola Lusvardi, Jorge Merino-Gutierrez, Daniela Carta","doi":"10.1039/d5tb00454c","DOIUrl":"https://doi.org/10.1039/d5tb00454c","url":null,"abstract":"<p><p>Phosphate-based glasses (PGs) are promising bioresorbable materials for controlled delivery of therapeutic species and tissue regeneration. The traditional method of synthesis of PGs involves the use of high temperatures, which limits their biomedical applications. The main goal of this work was to manufacture Mn loaded PGs for bone regeneration using an alternative, versatile and sustainable manufacturing technique. In this work, the novel room temperature, water-based method of coacervation was used for the synthesis of PGs in the system P₂O₅-CaO-Na₂O-(MnO)_<i>x</i> where <i>x</i> = 0, 1, 3, 5, 10 mol% both in powder (PGPs) and fibre (PGFs) form. PGPs were manufactured by vacuum drying polyphosphate coacervate gels and PGFs by electrospinning them. The addition of Mn²⁺, which plays an important role in bone mineralization, represents a clear novelty of this work as Mn loaded PGs prepared <i>via</i> coacervation have not been presented to date. Mn²⁺ release in deionized (DI) water has been shown to increase with Mn²⁺ loading in both PGPs and PGFs, demonstrating tailored release by modifying its content in the glass. <i>In vitro</i> biocompatibility was investigated for both systems <i>via</i> MTT assay on human osteosarcoma cells (MG-63) at three different ratios of dissolution products to cell medium after 24 h immersion in DI water (1, 3 and 5% v/v). Results have demonstrated that PGPs and PGFs loaded with Mn²⁺ up to 1 mol% are the most promising systems as they are not cytotoxic at all ratios investigated. Preliminary bioactivity tests performed by immersing a PGP sample containing 1 mol% of Mn²⁺ in both cell medium (McCoy's 5A) and Tris-buffer solution for 24 and 72 h suggest the deposition of a disordered, possibly hydroxyapatite-like phase on the surface of the glass. This study demonstrates that PGPs and PGFs, synthesised <i>via</i> coacervation, exhibit controlled release of the therapeutic ion Mn²⁺ and promising biocompatibility, making them suitable candidates for applications such as bone regeneration and controlled delivery.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144546590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue-adhesive, antibacterial, and macrophage-reprogramming hydrogel for sealing colorectal anastomotic leakage and promoting healing.","authors":"Yuzhou Zhu, Zhongwu Bei, Tianying Luo, Ziqiang Wang, Zhiyong Qian","doi":"10.1039/d5tb01242b","DOIUrl":"https://doi.org/10.1039/d5tb01242b","url":null,"abstract":"<p><p>Intestinal anastomosis is indispensable for treating inflammatory bowel disease and colorectal cancer, yet anastomotic leakage (AL) remains a frequent, life-threatening complication that markedly prolongs hospitalization. Conventional suturing affords limited protection against leakage and infection. Here, we report an injectable, multifunctional hydrogel (PGOT) composed of γ-poly(glutamic acid) conjugated with L-cysteine and dopamine (γ-PGA-Cys-DA), oxidized konjac glucomannan (OKGM), and tannic acid (TA). Dynamic thiol-aldehyde cross-linking affords rapid gelation (∼12 s) and repeatable self-healing, while catechol and polyphenol moieties confer strong wet-tissue adhesion and mechanical resilience that tolerates intestinal peristalsis and luminal pressure. <i>In vitro</i> and <i>in vivo</i> studies show that PGOT exhibits broad-spectrum antibacterial activity, robust pro-angiogenic properties, and effectively reprograms macrophages toward an M2 reparative phenotype. Application of PGOT to a rat colorectal anastomosis significantly reduces the incidence of AL and accelerates regenerative healing. These findings demonstrate that PGOT provides immediate mechanical sealing combined with bioactive regulation of the wound microenvironment, offering a promising strategy to improve postoperative outcomes after intestinal anastomosis.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144546593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}