Journal of biophotonics最新文献_第9页

High-Resolution Terahertz Spectroscopy for Medical Diagnostics. 用于医学诊断的高分辨率太赫兹光谱学。

Journal of biophotonics Pub Date : 2024-10-12 DOI: 10.1002/jbio.202400316

Vladimir Vaks, Elena Domracheva, Maria Chernyaeva, Vladimir Anfertev, Andrey Ayzenshtadt, Kseniya Glushkova, Aleksandra Cherniaeva

{"title":"High-Resolution Terahertz Spectroscopy for Medical Diagnostics.","authors":"Vladimir Vaks, Elena Domracheva, Maria Chernyaeva, Vladimir Anfertev, Andrey Ayzenshtadt, Kseniya Glushkova, Aleksandra Cherniaeva","doi":"10.1002/jbio.202400316","DOIUrl":"https://doi.org/10.1002/jbio.202400316","url":null,"abstract":"The metabolomics-based approach to diagnostics and therapy monitoring is a fast-emerging trend in modern medicine. Terahertz nonstationary spectroscopy based on the induction and disintegration of freely decaying polarization in the gas mixture during the interaction of radiation with molecules at resonance frequencies is a high-sensitivity method for studying multicomponent gas mixtures, which is promising for identifying metabolites in the thermal decomposition products of biological samples. The paper presents the results of the application of high-resolution terahertz spectroscopy to the study of biological samples taken from patients with certain diseases (pathologically changed tissues of ear-nose-throat organs and similar pathologic tissues formed in other life support systems) to search for characteristic sets of metabolites characterizing the pathology. The world's first measurements of the spectra of pathologic samples of cysts, formed in different life support systems, were carried out, which made it possible to identify similar substances in tissues having the same pathology.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400316"},"PeriodicalIF":0.0,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Simple and Fast Optical Clearing Method for Whole-Mount Fluorescence In Situ Hybridization (FISH) Imaging. 用于整装荧光原位杂交 (FISH) 成像的简单快速光学清除方法。

Journal of biophotonics Pub Date : 2024-10-10 DOI: 10.1002/jbio.202400258

Junwoo Suh, Yehe Liu, Jordan Smith, Michiko Watanabe, Andrew M Rollins, Michael W Jenkins

{"title":"A Simple and Fast Optical Clearing Method for Whole-Mount Fluorescence In Situ Hybridization (FISH) Imaging.","authors":"Junwoo Suh, Yehe Liu, Jordan Smith, Michiko Watanabe, Andrew M Rollins, Michael W Jenkins","doi":"10.1002/jbio.202400258","DOIUrl":"10.1002/jbio.202400258","url":null,"abstract":"We report a single-step optical clearing method that is compatible with RNA fluorescence in situ hybridization (FISH) imaging. We previously demonstrated microscopy imaging with immunohistochemistry and genetic reporters using a technique called lipid-preserving refractive index matching for prolonged imaging depth (LIMPID). Our protocol reliably produces high-resolution three-dimensional (3D) images with minimal aberrations using high magnification objectives, captures large field-of-view images of whole-mount tissues, and supports co-labeling with antibody and FISH probes. We also custom-designed FISH probes for quail embryos, demonstrating the ease of fabricating probes for use with less common animal models. Furthermore, we show high-quality 3D images using a conventional fluorescence microscope, without using more advanced depth sectioning instruments such as confocal or light-sheet microscopy. For broader adoption, we simplified and optimized 3D-LIMPID-FISH to minimize the barrier to entry, and we provide a detailed protocol to aid users with navigating the thick and thin of 3D microscopy.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400258"},"PeriodicalIF":0.0,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142402504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Graphene Oxide Nanoparticles on In Vitro Development of a Mouse Preimplantation Embryo and Interaction With the Zona Pellucida. 氧化石墨烯纳米粒子对小鼠植入前胚胎体外发育的影响以及与透明带的相互作用

Journal of biophotonics Pub Date : 2024-10-01 DOI: 10.1002/jbio.202400268

Artashes V Karmenyan, Alexander S Krivokharchenko, Micahella N Sarmiento, Eviyona L Barus, Elena V Perevedentseva, Chia-Liang Cheng

{"title":"Impact of Graphene Oxide Nanoparticles on In Vitro Development of a Mouse Preimplantation Embryo and Interaction With the Zona Pellucida.","authors":"Artashes V Karmenyan, Alexander S Krivokharchenko, Micahella N Sarmiento, Eviyona L Barus, Elena V Perevedentseva, Chia-Liang Cheng","doi":"10.1002/jbio.202400268","DOIUrl":"https://doi.org/10.1002/jbio.202400268","url":null,"abstract":"The development of assisted reproductive technologies increases the likelihood of nanoparticles' (NPs) direct contact with gametes and embryos in in vitro conditions. Analyzing the influence of nanomaterials on the early mammalian embryo becomes increasingly relevant. This work is devoted to the effect of graphene oxide (GO) NPs on the in vitro development of mammalian embryos. Mouse 2-cell embryos were preincubated with GO NPs. The interaction of GO with the Zona Pellucida (ZP) of the embryo was investigated using fluorescence lifetime imaging with two-photon excitation (2p-FLIM). During embryo development, the NPs penetration into ZP (blastocyst stage) and perivitelline space (blastocyst hatching stage) was observed. Despite this, GO did not affect the embryo's ability to develop till late and hatching blastocysts. The mechanism of the NPs getting into the perivitelline space and the consequences of NP-embryo direct contact are discussed. The 2p-FLIM efficiency for studying NP interaction with mammalian embryos is evaluated.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400268"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photoplethysmography-Based Angiography of Skin Tumors in Arbitrary Areas of Human Body. 基于光速摄影的人体任意部位皮肤肿瘤血管造影。

Journal of biophotonics Pub Date : 2024-09-26 DOI: 10.1002/jbio.202400242

Anastasia Guryleva, Alexander Machikhin, Ekaterina Orlova, Evgeniya Kulikova, Michail Volkov, Gaiane Gabrielian, Ludmila Smirnova, Marina Sekacheva, Olga Olisova, Ekaterina Rudenko, Olga Lobanova, Vera Smolyannikova, Tatiana Demura

{"title":"Photoplethysmography-Based Angiography of Skin Tumors in Arbitrary Areas of Human Body.","authors":"Anastasia Guryleva, Alexander Machikhin, Ekaterina Orlova, Evgeniya Kulikova, Michail Volkov, Gaiane Gabrielian, Ludmila Smirnova, Marina Sekacheva, Olga Olisova, Ekaterina Rudenko, Olga Lobanova, Vera Smolyannikova, Tatiana Demura","doi":"10.1002/jbio.202400242","DOIUrl":"https://doi.org/10.1002/jbio.202400242","url":null,"abstract":"Noninvasive, rapid, and robust diagnostic techniques for clinical screening of tumors located in arbitrary areas of the human body are in demand. To address this challenge, we analyzed the feasibility of photoplethysmography-based angiography for assessing vascular structures within malignant and benign tumors. The proposed hardware and software were approved in a clinical study involving 30 patients with tumors located in the legs, torso, arms, and head. High-contrast and detailed vessel maps within both benign and malignant tumors were obtained. We demonstrated that capillary maps are consistent and can be interpreted using well-established dermoscopic criteria for vascular morphology. Vessel mapping provides valuable details, which may not be available in dermoscopic images and can aid in determining whether a tumor is benign or malignant. We believe that the proposed approach may become a valuable tool in the preliminary cancer diagnosis and is suitable for large-scale screening.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400242"},"PeriodicalIF":0.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microsaccades Tracking by Secondary Speckle Pattern Analysis. 通过二次斑点模式分析追踪微注视

Journal of biophotonics Pub Date : 2024-09-09 DOI: 10.1002/jbio.202400184

Ola Shteinberg, Sergey Agdarov, Yafim Beiderman, Yoram S Bonneh, Inbal Ziv, Zeev Zalevsky

引用次数: 0

Extracting Superficial Scattering by Q-Sensing Technique. 利用 Q 感应技术提取表层散射。

Journal of biophotonics Pub Date : 2024-09-06 DOI: 10.1002/jbio.202400262

Alon Tzroya, Hamootal Duadi, Dror Fixler

{"title":"Extracting Superficial Scattering by Q-Sensing Technique.","authors":"Alon Tzroya, Hamootal Duadi, Dror Fixler","doi":"10.1002/jbio.202400262","DOIUrl":"https://doi.org/10.1002/jbio.202400262","url":null,"abstract":"Optical properties determine how light interacts with biological tissues. The current methods for measuring these optical properties are influenced by both deep and superficial skin layers. Polarization-based methods have been proposed in order to determine the influence of deep layer scattering. Polarized light allows for the separation of ballistic photons from diffuse ones, enhancing image contrast and resolution while providing additional tissue information. The Q-sensing technique captures co-polarized <math> <semantics> <mrow> <mfenced><msub><mi>I</mi> <mo>∥</mo></msub> </mfenced> </mrow> <annotation>$$ left({I}_{parallel}right) $$</annotation></semantics> </math> and cross-polarized <math> <semantics> <mrow> <mfenced><msub><mi>I</mi> <mo>⊥</mo></msub> </mfenced> </mrow> <annotation>$$ left({I}_{perp}right) $$</annotation></semantics> </math> signals, making it possible to isolate the superficial scattering. However, the random structure of tissues leads to rapid depolarization of the polarized light. Detecting where the light becomes depolarized aids in sensing abnormalities within the tissues. Hence, this research focuses on identifying where depolarization occurs within the tissue. Tissue-mimicking phantoms, simulating the optical properties of biological tissues, are created to measure depolarization at various thicknesses. Experimental findings are validated with a Monte Carlo simulation, modeling polarized light behavior through the polydisperse tissue (as the tissue scatterers are heterogeneous in size). Additionally, the research demonstrates how polarized light can extract the optical properties of the medium.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400262"},"PeriodicalIF":0.0,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142143508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of the Effect of Compression Pressure in Contact OCT Imaging on the Measurement of Epidermis Thickness. 研究接触式 OCT 成像中的压缩压力对表皮厚度测量的影响

Journal of biophotonics Pub Date : 2024-08-27 DOI: 10.1002/jbio.202400289

Zhiying Xie, Yaping Shi, Agathe Marmin, Ruikang K Wang

引用次数: 0

Incorporating artificial intelligence in portable infrared thermal imaging for the diagnosis and staging of nonalcoholic fatty liver disease. 将人工智能纳入便携式红外热成像，用于非酒精性脂肪肝的诊断和分期。

Journal of biophotonics Pub Date : 2024-08-06 DOI: 10.1002/jbio.202400189

Yana Davidov, Rafael Y Brzezinski, Monica-Inda Kaufmann, Mariya Likhter, Tammy Hod, Orit Pappo, Yair Zimmer, Zehava Ovadia-Blechman, Neta Rabin, Adi Barlev, Orli Berman, Ziv Ben Ari, Oshrit Hoffer

{"title":"Incorporating artificial intelligence in portable infrared thermal imaging for the diagnosis and staging of nonalcoholic fatty liver disease.","authors":"Yana Davidov, Rafael Y Brzezinski, Monica-Inda Kaufmann, Mariya Likhter, Tammy Hod, Orit Pappo, Yair Zimmer, Zehava Ovadia-Blechman, Neta Rabin, Adi Barlev, Orli Berman, Ziv Ben Ari, Oshrit Hoffer","doi":"10.1002/jbio.202400189","DOIUrl":"https://doi.org/10.1002/jbio.202400189","url":null,"abstract":"Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) is one of the most prevalent chronic liver diseases worldwide. Thermal imaging combined with advanced image-processing and machine learning analysis accurately classified disease status in a study on mice; this study aimed to develop this tool for humans. This prospective study included 46 patients who underwent liver biopsy. Liver thermal imaging was performed on the same day as liver biopsy. We developed an image-processing algorithm that measured the relative spatial thermal variation across the skin covering the liver. The texture parameters obtained from the thermal images were input into the machine learning algorithm. Patients were diagnosed with MASLD and stratified according to nonalcoholic fatty liver disease activity score (NAS) and fibrosis stage using the METAVIR score. Twenty-one of 46 patients were diagnosed with MASLD. Using thermal imaging followed by processing, detection accuracy for patients with NAS >4 was 0.72.","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202400189"},"PeriodicalIF":0.0,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141899249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of image formation in optical palpation. 光学触诊图像形成分析。

Journal of biophotonics Pub Date : 2024-07-30 DOI: 10.1002/jbio.202400180

R Jones, Q Fang, B F Kennedy

引用次数: 0

Delineation of gastrointestinal tumors biopsies using a fluorescence lifetime imaging optical fiber probe. 利用荧光寿命成像光纤探针划分胃肠道肿瘤活检组织。

Journal of biophotonics Pub Date : 2024-07-16 DOI: 10.1002/jbio.202400122

D Suraci, E Baria, L Tirloni, J L Lagarto, S Buccianti, C Agostini, S Pillozzi, L Antonuzzo, A Taddei, R Cicchi

引用次数: 0