Optimized reconstruction of the absorption spectra of kidney tissues from the spectra of tissue components using the least squares method.

Maria R Pinheiro, Luís E Fernandes, Isa C Carneiro, Sónia D Carvalho, Rui M Henrique, Valery V Tuchin, Hélder P Oliveira, Luís M Oliveira
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Abstract

With the objective of developing new methods to acquire diagnostic information, the reconstruction of the broadband absorption coefficient spectra (μa [λ]) of healthy and chromophobe renal cell carcinoma kidney tissues was performed. By performing a weighted sum of the absorption spectra of proteins, DNA, oxygenated, and deoxygenated hemoglobin, lipids, water, melanin, and lipofuscin, it was possible to obtain a good match of the experimental μa (λ) of both kidney conditions. The weights used in those reconstructions were estimated using the least squares method, and assuming a total water content of 77% in both kidney tissues, it was possible to calculate the concentrations of the other tissue components. It has been shown that with the development of cancer, the concentrations of proteins, DNA, oxygenated hemoglobin, lipids, and lipofuscin increase, and the concentration of melanin decreases. Future studies based on minimally invasive spectral measurements will allow cancer diagnosis using the proposed approach.

利用最小二乘法从组织成分的光谱中优化重建肾脏组织的吸收光谱。
为了开发获取诊断信息的新方法,研究人员重建了健康肾脏组织和嗜色性肾细胞癌肾脏组织的宽带吸收系数光谱(μa [λ])。通过对蛋白质、DNA、含氧血红蛋白和脱氧血红蛋白、脂质、水、黑色素和脂褐素的吸收光谱进行加权求和,可以获得两种肾脏状况下实验μa (λ) 的良好匹配。使用最小二乘法估算了这些重构所使用的重量,并假设两种肾组织的总含水量为 77%,从而可以计算出其他组织成分的浓度。研究表明,随着癌症的发展,蛋白质、DNA、含氧血红蛋白、脂类和脂褐素的浓度会增加,而黑色素的浓度会降低。未来基于微创光谱测量的研究将允许使用建议的方法进行癌症诊断。
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