电子烟液体对猪肺组织的影响——离体共聚焦拉曼显微光谱研究。

Ali Jaafar, Abbas Albarazanchi, Mohammed Jawad Kadhim, Maxim E Darvin, Tamás Váczi, Valery V Tuchin, Miklós Veres
{"title":"电子烟液体对猪肺组织的影响——离体共聚焦拉曼显微光谱研究。","authors":"Ali Jaafar,&nbsp;Abbas Albarazanchi,&nbsp;Mohammed Jawad Kadhim,&nbsp;Maxim E Darvin,&nbsp;Tamás Váczi,&nbsp;Valery V Tuchin,&nbsp;Miklós Veres","doi":"10.1002/jbio.202300336","DOIUrl":null,"url":null,"abstract":"<p><p>Ex vivo porcine lung immersed in e-liquid was investigated in-depth using confocal Raman micro-spectroscopy to assess the e-liquid influence on the lung. It was found that lung-related Raman band intensities at 1002, 1548, 1618 and 1655 cm<sup>-1</sup> increased after first and second treatments except the surface, which was attributed to the well-known optical clearing (OC) effect due to alveoli filling with e-liquid resulting in light scattering reduction. The autofluorescence enhancement was explained by oxidative stress induced in lung during exposure to e-liquid. Moreover, e-liquid induced collagen dehydration was revealed by the I<sub>937</sub> /I<sub>926</sub> Raman band intensity ratio change. The effect was enhanced after the second treatment of the same lung tissue that indicates the possibility of multi-step OC treatment. We hypothesize that the nicotine-flavour-free e-liquids containing glycerol and propylene glycol could potentially be used in clinical protocols as OC agent for enhanced in-depth Raman-guided bronchoscopy.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Impact of e-cigarette liquid on porcine lung tissue-Ex vivo confocal Raman micro-spectroscopy study.\",\"authors\":\"Ali Jaafar,&nbsp;Abbas Albarazanchi,&nbsp;Mohammed Jawad Kadhim,&nbsp;Maxim E Darvin,&nbsp;Tamás Váczi,&nbsp;Valery V Tuchin,&nbsp;Miklós Veres\",\"doi\":\"10.1002/jbio.202300336\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Ex vivo porcine lung immersed in e-liquid was investigated in-depth using confocal Raman micro-spectroscopy to assess the e-liquid influence on the lung. It was found that lung-related Raman band intensities at 1002, 1548, 1618 and 1655 cm<sup>-1</sup> increased after first and second treatments except the surface, which was attributed to the well-known optical clearing (OC) effect due to alveoli filling with e-liquid resulting in light scattering reduction. The autofluorescence enhancement was explained by oxidative stress induced in lung during exposure to e-liquid. Moreover, e-liquid induced collagen dehydration was revealed by the I<sub>937</sub> /I<sub>926</sub> Raman band intensity ratio change. The effect was enhanced after the second treatment of the same lung tissue that indicates the possibility of multi-step OC treatment. We hypothesize that the nicotine-flavour-free e-liquids containing glycerol and propylene glycol could potentially be used in clinical protocols as OC agent for enhanced in-depth Raman-guided bronchoscopy.</p>\",\"PeriodicalId\":94068,\"journal\":{\"name\":\"Journal of biophotonics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-10-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of biophotonics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1002/jbio.202300336\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biophotonics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/jbio.202300336","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

使用共聚焦拉曼显微光谱对浸泡在电子液体中的离体猪肺进行了深入研究,以评估电子液体对肺的影响。研究发现,除表面外,第一次和第二次处理后,1002、1548、1618和1655 cm-1处的肺相关拉曼光谱强度增加,这归因于众所周知的光学清除效应,因为肺泡充满电子液体,导致光散射减少。自发荧光增强是由暴露于电子液体期间在肺部诱导的氧化应激所解释的。此外,I937/I926拉曼带强度比的变化揭示了电子液体诱导的胶原脱水。对同一肺组织进行第二次治疗后,效果增强,这表明有可能进行多步骤OC治疗。我们假设,含有甘油和丙二醇的无尼古丁香料电子液体有可能在临床方案中用作增强深度拉曼引导支气管镜检查的光学清除剂。这篇文章受版权保护。保留所有权利。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Impact of e-cigarette liquid on porcine lung tissue-Ex vivo confocal Raman micro-spectroscopy study.

Impact of e-cigarette liquid on porcine lung tissue-Ex vivo confocal Raman micro-spectroscopy study.

Ex vivo porcine lung immersed in e-liquid was investigated in-depth using confocal Raman micro-spectroscopy to assess the e-liquid influence on the lung. It was found that lung-related Raman band intensities at 1002, 1548, 1618 and 1655 cm-1 increased after first and second treatments except the surface, which was attributed to the well-known optical clearing (OC) effect due to alveoli filling with e-liquid resulting in light scattering reduction. The autofluorescence enhancement was explained by oxidative stress induced in lung during exposure to e-liquid. Moreover, e-liquid induced collagen dehydration was revealed by the I937 /I926 Raman band intensity ratio change. The effect was enhanced after the second treatment of the same lung tissue that indicates the possibility of multi-step OC treatment. We hypothesize that the nicotine-flavour-free e-liquids containing glycerol and propylene glycol could potentially be used in clinical protocols as OC agent for enhanced in-depth Raman-guided bronchoscopy.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信