Experimental and therapeutic medicine最新文献

{"title":"[Retracted] MicroRNA-140-5p regulates the proliferation, apoptosis and inflammation of RA FLSs by repressing STAT3.","authors":"Jiehua Zhu, Jianglin Wang, Jialin Huang, Wensheng Du, Yingzhong He, Hongfei Pan, Junmin Luo","doi":"10.3892/etm.2026.13147","DOIUrl":"10.3892/etm.2026.13147","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.3892/etm.2020.9602.].</p>","PeriodicalId":94002,"journal":{"name":"Experimental and therapeutic medicine","volume":"31 6","pages":"152"},"PeriodicalIF":2.3,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13112362/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147792884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interacting proteins of AMPK studied using TurboID proximity labeling technology.","authors":"Xiaoshan Liu, Jieyu Guo, Qiongyao Wang, Wu Liu, Yan Xue, Shuang Guo, Miao Li","doi":"10.3892/etm.2026.13144","DOIUrl":"10.3892/etm.2026.13144","url":null,"abstract":"<p><p>In living organisms, the change in the adenosine 5'-monophosphate (AMP)/adenosine 5'-triphosphate ratio serves as a key signal regulating AMP-activated protein kinase (AMPK) activity, which is an important molecule for the control of cellular energy metabolism and serves a key role in a number of diseases, such as diabetes and myocardial infarction. To explore novel AMPK-interacting proteins and investigate their biological functions, the present study used TurboID proximity labeling in U251 cells stably expressing AMPK-TurboID. Novel AMPK-interacting proteins were explored under both normal and CCCP-treated conditions to investigate their biological functions. Stable cell lines overexpressing AMPK-TurboID were successfully established, and interacting proteins were identified through biotin labeling, silver staining and mass spectrometry analysis. Due to the involvement of AMPK in numerous metabolic pathways and the advantages of TurboID over traditional techniques, including its high temporal resolution, <i>in situ</i> labeling in living cells and its ability to capture weak and transient interactions, a number of proteins interacting with AMPK were identified. Six proteins identified by label-free MS were validated by western blotting, showing that the expression levels of menage a trois-1 and DNAJ heat shock protein family (Hsp40) member A1 (DNAJA1) closely matched the MS data. DNAJA1 was selected for further experimentation. TurboID proximity labeling, affinity purification and LC-MS/MS, western blotting, co-immunoprecipitation (co-IP) and immunofluorescence (IF) techniques demonstrated that AMPK and DNAJA1 not only interact with each other but also synergistically protect cells from apoptosis, perhaps providing a basis for targeted therapeutics (such as treatments for diabetes and myocardial infarction). The present study achieved covalent labeling of endogenous proteins located within nanometer distance of the labeling enzyme by adding a biotin-based substrate. The labeled proteins were then captured and enriched using streptavidin-coated magnetic beads for subsequent identification by MS. These MS results, combined with subsequent validation, led to the identification of DNAJA1 as a key AMPK-interacting protein that synergizes with AMPK to protect cells from apoptosis.</p>","PeriodicalId":94002,"journal":{"name":"Experimental and therapeutic medicine","volume":"31 6","pages":"149"},"PeriodicalIF":2.3,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13096766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147793011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of <i>rok1</i> gene deletion on mitosis in fission yeast at appropriate and stressful temperatures and the molecular mechanisms.","authors":"Jiayi He, Mengnan Liu, Jiani Xu, Xiang Ding, Yiling Hou","doi":"10.3892/etm.2026.13145","DOIUrl":"10.3892/etm.2026.13145","url":null,"abstract":"<p><p>The <i>rok1</i> gene encodes the ATP-dependent RNA helicase Rok1, which is involved in regulating the maturation of small subunit ribosomal RNA and thus ribosome biogenesis. However, the regulation of cellular mitotic dynamics by the <i>rok1</i> gene deletion is currently unclear. In the present study, fluorescent protein labeling and live cell imaging techniques were used to investigate the effects of <i>rok</i>1 deletion on the dynamics of microtubules, actin and kinetochores during mitosis at 25 and 37˚C, and RNA-sequencing and bioinformatics analyses were used to reveal the key genes. Analysis of the live cell imaging results revealed that, in mitosis, the initiation length and contraction length of actin rings were both shortened and the contraction rate was decreased at 25 and 37˚C. The separation process of kinetochores was inhibited at 25 and 37˚C, and the inhibition was more severe at the higher temperature of 37˚C. Analysis of RNA sequencing results showed that upregulation of <i>myo51</i> and <i>blt1</i> resulted in delayed actin ring assembly and slowed actin ring contraction in the <i>rok1</i>Δ strain. In addition, <i>psm1</i> and <i>psc3</i> were upregulated and are key genes affecting the ability of kinetochores to move on the spindle and the cohesion of sister chromatids. The present study revealed that the Rok1 protein not only influences the actin polymerization process, participate in the regulation of actin ring assembly and contraction, and cytoplasmic division, but also affects the migration ability of kinetochores on the spindle and participate in the regulation of the formation and maintenance of cohesion between sister chromatids, which provides a certain scientific basis for further exploring the function of the Rok1 protein in cell division.</p>","PeriodicalId":94002,"journal":{"name":"Experimental and therapeutic medicine","volume":"31 6","pages":"151"},"PeriodicalIF":2.3,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13093012/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147793004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of allicin against viral infections: Mechanisms and safety profile (Review).","authors":"Ming-Jun Yu, Yong Zhu, Wei Zhang, Qiao-Ping Xu, Hai-Ming Wang, Zhao Ma","doi":"10.3892/etm.2026.13146","DOIUrl":"10.3892/etm.2026.13146","url":null,"abstract":"<p><p>For centuries, garlic and other <i>Allium</i> species have been valued not only as food but also for their medicinal uses, with their antiviral effects recorded in numerous traditional healing practices. Crushing or chopping of garlic releases potent organosulfur compounds, the most notable of which is allicin, a compound that fights a range of viruses, including influenza and herpes simplex virus. These organosulfur compounds, particularly allicin and its derivatives such as diallyl disulfide, diallyl trisulfide and ajoene, function through a number of mechanisms, preventing viral entry into host cells by blocking attachment and fusion, inhibiting key viral enzymes required for replication (such as neuraminidase and viral polymerase), and strengthening the innate immune response of the host. However, the clinical application of allicin has been limited by its inherent instability, rapid metabolism and poor bioavailability. To overcome these challenges, researchers are beginning to develop innovative nano-delivery systems using lipid nanoparticles and polymeric carriers that can effectively protect allicin from degradation, improving its solubility and enhancing targeted delivery to infected tissues. Combining the established applications of allicin with nanotechnology advancements represents a promising way to create garlic-based antiviral treatments. However, their effectiveness still needs to be determined through rigorous clinical trials in order to develop antiviral therapies that may finally realize the full therapeutic potential long attributed to this ancient remedy.</p>","PeriodicalId":94002,"journal":{"name":"Experimental and therapeutic medicine","volume":"31 6","pages":"150"},"PeriodicalIF":2.3,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13093131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147793037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics analysis of glutamine and fish collagen peptides in alleviating post-antibiotic <i>Streptococcus pneumoniae</i> injury in feline lung cells.","authors":"Huasong Bai, Tong Liu, Hengyan Wang, Hongchen Jin, Yunliang Li, Zhanzhong Wang","doi":"10.3892/etm.2026.13143","DOIUrl":"10.3892/etm.2026.13143","url":null,"abstract":"<p><p><i>Streptococcus pneumoniae</i> (SP) infection often leads to persistent lung injury even after antibiotic treatment. Despite this phenomenon, the mechanisms underlying host cell recovery remain poorly understood. Upon breaching the epithelial barrier, SP primarily targets the pulmonary interstitial cells, which constitute the major mesenchymal component of the lung. These cells serve as essential effectors of tissue repair, extracellular matrix remodeling and epithelial restoration. Therefore, a feline pulmonary interstitial cell (FCA-L2) model of SP infection was established to investigate the protective effects of glutamine (GLU) and fish collagen peptides (FCP) through integrated transcriptomic and metabolomic analyses. Cells were infected with SP (0.05 McFarland units for 4 h) and then treated with doxycycline (7.5 µg/ml for 18 h) followed by GLU (40 mM) or FCP (500 µg/ml). Notably, SP infection increased lactate dehydrogenase (LDH) release by 3.5-fold, induced secretion of IL-1β, TNF-α and IL-8, disrupted tight-junction proteins (claudin, ZO-1 and occludin) and caused oxidative imbalance and apoptosis despite antibiotic (doxycycline) treatment. However, treatment with GLU or FCP significantly reduced LDH release by ~40%, restored junctional proteins, suppressed inflammatory cytokines and enhanced antioxidant enzyme activities. Multi-omics analysis revealed that GLU promoted amino acid biosynthesis and energy metabolism and suppressed aminoacyl-tRNA synthetases and cell-cycle regulators, thereby enhancing metabolic adaptability. By contrast, FCP activated amino and nucleotide sugar metabolism, increased polyunsaturated fatty-acid synthesis and supported glycocalyx repair and membrane reconstruction. GLU and FCP provided complementary metabolic and structural protection, which mitigated post-infectious stress and promoted cellular recovery. The findings of the present study underscore the potential of bioactive food-derived compounds as adjunctive therapies that may accelerate lung tissue repair and enhance the efficacy of conventional antibiotics.</p>","PeriodicalId":94002,"journal":{"name":"Experimental and therapeutic medicine","volume":"31 6","pages":"148"},"PeriodicalIF":2.3,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13077270/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147694406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Retracted] MicroRNA-1236-3p/translationally controlled tumor protein (TPT1) axis participates in congenital hypothyroidism progression by regulating neuronal apoptosis.","authors":"Tingting Meng, Shiman Shen, Cheng Li, Xuehua Liu","doi":"10.3892/etm.2026.13142","DOIUrl":"10.3892/etm.2026.13142","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.3892/etm.2019.8262.].</p>","PeriodicalId":94002,"journal":{"name":"Experimental and therapeutic medicine","volume":"31 6","pages":"147"},"PeriodicalIF":2.3,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13082828/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147701278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>FCER1G</i>: A multifunctional regulator in the immune microenvironment (Review).","authors":"Yuyu Zhang, Jinglan Wang, Wenting He, Tao Liu","doi":"10.3892/etm.2026.13141","DOIUrl":"10.3892/etm.2026.13141","url":null,"abstract":"<p><p>The Fc fragment of IgE receptor 1G (<i>FCER1G</i>) gene encodes the Fc receptor common γ chain (FcRγ), a crucial adaptor protein in immune signaling. As the core subunit for multiple immune receptors, including high-affinity immunoglobulin E receptor, Fc fragment of IgG receptor and C-type lectin receptors, it regulates both innate and adaptive immune responses through its intracellular immunoreceptor tyrosine-based activation motif. Downstream effector functions include antibody-dependent cytotoxicity, phagocytosis and inflammatory cytokine production. Emerging evidence implicates <i>FCER1G</i> in diverse pathological conditions. In inflammatory diseases, epigenetic mechanisms strictly regulate its expression, driving inflammation by influencing immune cell polarization. In the context of cancer, it induces tumor progression by remodeling the immune microenvironment, inducing angiogenesis and enabling platelet-mediated metastasis. Notably, its prognostic value varies according to tissue origin. Although no current drugs directly target <i>FCER1G</i>, established agents such as aspirin may indirectly modulate its signaling. The present review aimed to summarize the current knowledge on the molecular structure, immune functions and regulatory mechanisms of <i>FCER1G</i> in inflammation and carcinogenesis, establishing a rationale for its potential use as a prognostic biomarker and therapeutic target.</p>","PeriodicalId":94002,"journal":{"name":"Experimental and therapeutic medicine","volume":"31 5","pages":"146"},"PeriodicalIF":2.3,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13082826/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147701313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A nomogram for predicting recurrence-free survival in patients with high-risk GIST receiving adjuvant imatinib: A nationwide registry study.","authors":"Chun-Nan Yeh, Yan-Shen Shan, Ching-Yao Yang, Chin-Fu Hsiao, Chung-Hsin Tsai, Chuan-Cheng Wang, Ming-Tsan Lin, Chun-Fu Ting, De-Chuan Chan, Te-Hung Chen, Chueh-Chuan Yen, Yen-Yang Chen, Hsuan-Yu Lin, Ta-Sen Yeh, Ching-Liang Ho, Tze-Yu Shieh, Li-Yaun Bai, Jun-Te Hsu, I-Shu Chen, Li-Tzong Chen, Hui-Jen Tsai","doi":"10.3892/etm.2026.13140","DOIUrl":"10.3892/etm.2026.13140","url":null,"abstract":"<p><p>Despite adjuvant imatinib therapy, a substantial proportion of patients with high-risk gastrointestinal stromal tumor (GIST) experience disease recurrence. This study aimed to identify prognostic factors associated with poor recurrence-free survival (RFS) and develop a predictive nomogram for personalized clinical decision-making. In the present study, a retrospective analysis was conducted of 269 patients with modified Armed Forces Institute of Pathology-classified high-risk GIST who underwent macroscopically complete resection and received adjuvant imatinib therapy at 11 Taiwanese centers between 2013 and 2023. All patients were intended to receive 3 years of adjuvant imatinib and were categorized into disease-free (n=204) and recurrence (n=65) cohorts. Independent predictors of poor RFS were identified through multivariate Cox regression analysis and incorporated into a nomogram. Model performance was assessed using concordance index (C-index) and calibration plots. The cohort comprised 149 males and 120 females with a median age of 60.9 years (range, 29-88 years). At a median follow-up of 62.6 months, the 1-, 3- and 5-year disease-free survival rates were 97.0, 91.7 and 76.4%, respectively. Multivariate analysis identified male sex [hazard ratio (HR): 1.76, P=0.039], non-gastric tumor origin with duodenum showing highest risk (HR: 6.15, P<0.0001) and shorter imatinib duration with <1 year treatment showing HR: 3.91 (P=0.002) as independent predictors of poor RFS. The resulting nomogram demonstrated robust predictive performance with good calibration and C-index of 0.72. Nomogram-based prognostic stratification identified three distinct groups with 5-year survival rates of 99.2% (favorable prognosis), 68.1% (moderate prognosis) and 16.7% (poor prognosis). In the present study, a clinically applicable nomogram was developed that accurately predicts poor RFS in patients with high-risk GIST following surgery and adjuvant imatinib therapy. While external validation is needed before clinical implementation, this prognostic tool may facilitate individualized treatment strategies and patient counseling.</p>","PeriodicalId":94002,"journal":{"name":"Experimental and therapeutic medicine","volume":"31 5","pages":"145"},"PeriodicalIF":2.3,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13044977/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147625254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Retracted] MicroRNA-18a-5p represses scar fibroblast proliferation and extracellular matrix deposition through regulating Smad2 expression.","authors":"Tianshi Li, Yiguang Wu, Dandan Liu, Lida Zhuang","doi":"10.3892/etm.2026.13139","DOIUrl":"https://doi.org/10.3892/etm.2026.13139","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.3892/etm.2021.10753.].</p>","PeriodicalId":94002,"journal":{"name":"Experimental and therapeutic medicine","volume":"31 5","pages":"144"},"PeriodicalIF":2.3,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13491531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148803590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors for severe retinopathy of prematurity: A retrospective case-control study.","authors":"Qingmin Ma, Xi Lv, Jiangya Wang, Kejun Li, Wenwen Tang","doi":"10.3892/etm.2026.13138","DOIUrl":"10.3892/etm.2026.13138","url":null,"abstract":"<p><p>The present study aimed to investigate the risk factors associated with severe retinopathy of prematurity (ROP) in preterm infants and to identify predictive biomarkers for early risk stratification. The present retrospective case-control study analyzed preterm infants who underwent ROP screening at Hebei General Hospital (Shijiazhuang, China) between September 2018 and July 2023. Participants were stratified into mild (n=42) and severe ROP (n=38) groups based on fundus examination results and treatment requirements. Demographic characteristics, perinatal complications, maternal third-trimester lipid profiles and serial hematological parameters (within 24 h of birth and at 1 week of age) were analyzed. Binary logistic regression analysis was performed to identify independent risk factors, and receiver operating characteristic (ROC) curves were constructed to evaluate predictive performance. Significant differences were observed between severe and mild ROP groups in gestational age, birth weight and 1-min Apgar score (all P<0.001). Perinatal complications significantly associated with severe ROP included neonatal bronchopulmonary dysplasia (P<0.001), neonatal respiratory distress syndrome (P=0.003), neonatal sepsis (P=0.009) and blood transfusion requirements (P<0.001). Among hematological parameters, lymphocyte-to-monocyte ratio (LMR) was significantly lower in the severe ROP group both within 24 h of birth (P=0.015) and at 1 week of age (P=0.01). Multivariate logistic regression identified gestational age as the sole independent risk factor for severe ROP (P<0.001). ROC curve analysis revealed that gestational age ≤30.5 weeks predicted severe ROP with 76.2% sensitivity and 71.1% specificity (P<0.001). Maternal third-trimester lipid parameters showed no significant associations with severe ROP development. In conclusion, gestational age represents the most significant independent predictor of severe ROP in preterm infants, with infants born at ≤30.5 weeks showing significantly increased risk. Additional risk factors include major perinatal complications and altered inflammatory profiles characterized by reduced LMR. These findings support enhanced surveillance protocols for preterm infants and suggest the potential utility of inflammatory biomarkers in ROP risk stratification strategies.</p>","PeriodicalId":94002,"journal":{"name":"Experimental and therapeutic medicine","volume":"31 5","pages":"143"},"PeriodicalIF":2.3,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13040524/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147611032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}