Experimental and therapeutic medicine最新文献

{"title":"Clinical characteristics and prognostic analysis of patients with HIV and glioma: A case series and literature review.","authors":"Xinghuan Ding, Tingyu Liang, Bo Liang, Xingang Zhou, Jiamin Chen, Haili Gao, Fang Wang, Xinmei Zheng, Enshan Feng","doi":"10.3892/etm.2024.12380","DOIUrl":"10.3892/etm.2024.12380","url":null,"abstract":"<p><p>Cerebral glial tumors have become increasingly common in human immunodeficiency virus (HIV)-positive patients. The present study aimed to report a series of such cases, explore their clinical and pathological characteristics and subject all the reported cases to a survival analysis. The characteristics, management and prognosis of 10 HIV-positive patients with brain gliomas enrolled in a single hospital were investigated in detail. Immunohistochemical assessment of CD31, CD68 and CD163 was performed in the 10 HIV-positive patients with glioma and 18 HIV-negative patients with glioma. The relevant literature was also reviewed using relevant search terms. The potential predictive factors were screened by univariate and multivariate logistic regression analyses, and a nomogram was established based on the potential predictive factors. A total of 50 patients, including the 10 primary cases, were included in the survival analysis. The median survival time was 9 months. The gliomas of HIV-negative patients had a lower cell count of CD163⁺ cells than those of HIV-positive patients. High CD4⁺ T-cell count and the use of highly active antiretroviral therapy (HAART) tended to increase the median survival duration, although not significantly according to the log-rank analysis. In the univariate analysis, only surgery, radiotherapy (RT) and World Health Organization (WHO) tumor grade had significant associations with overall survival. In the multivariate analysis, only RT and WHO grade were independent predictors. In conclusion, gliomas may occur more frequently in HIV-positive populations than is currently recognized. The survival duration of most HIV-positive patients with glioma is determined by the tumor rather than HIV status. Adjuvant radiotherapy and the WHO grade of the glioma are predicted to be independent prognostic factors. Surgical resection followed by RT plus regular HAART is recommended for patients with glioma who are HIV-positive.</p>","PeriodicalId":94002,"journal":{"name":"Experimental and therapeutic medicine","volume":"27 2","pages":"90"},"PeriodicalIF":0.0,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10809446/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139565125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemokine‑like receptor 1‑positive cells are present in the odontoblast layer in tooth tissue in rats and humans.","authors":"Daisuke Yokokawa, Naoki Umemura, Yuka Miyamoto, Nobuo Kondoh, Satoshi Kawano","doi":"10.3892/etm.2023.12363","DOIUrl":"10.3892/etm.2023.12363","url":null,"abstract":"<p><p>Cluster of differentiation (CD)44 is a marker of dental pulp stem cells and is involved in odontoblast differentiation and calcification. Chemokine-like receptor 1 (CMKLR1), also known as chemerin receptor 23 (ChemR23) is also expressed in odontoblasts and dental pulp stem cells and is involved in inflammation suppression and tooth regeneration. Resolvin E1, a bioactive lipid, is a CMKLR1 ligand that mediates the chemerin-CMKLR1 interaction and suppresses pulpal inflammation. The present study clarified the intracellular and tissue localization of CD44 and CMKLR1 by immunohistochemical staining of normal pulp and pulp with pulpitis from 12-week-old male Wistar rat teeth or human teeth. In addition, the localization of CD44 and CMKLR1 in human dental pulp stem cells was observed by immunofluorescence staining. The present study also examined the involvement of resolvin E1 in inhibiting inflammation and calcification by western blotting. CD44- and CMKLR1-positive cells were confirmed in the odontoblast layer in normal dental pulp of rats and humans. CD44 was mainly localized in the cell membrane and CMKLR1 was mainly found in the cytoplasm of human dental pulp stem cells. CMKLR1 was also confirmed in the odontoblast layer in rats and humans with pulpitis but CD44 was not present. Following treatment of dental pulp stem cells with lipoteichoic acid, which imitates Gram-positive bacterial infection, resolvin E1 did not suppress the expression of cyclooxygenase-2 or of the odontoblast differentiation marker, dentin sialophosphoprotein. Furthermore, resolvin E1 induced the differentiation of dental pulp stem cells into odontoblasts even in the presence of the inflammatory stimulus.</p>","PeriodicalId":94002,"journal":{"name":"Experimental and therapeutic medicine","volume":"27 2","pages":"75"},"PeriodicalIF":0.0,"publicationDate":"2023-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10804379/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139543854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum caspase‑1 levels serve as a predictive biomarker for the prognosis of patients undergoing arthroscopic‑assisted locking plate internal fixation in the treatment of high‑energy pilon fractures.","authors":"Xiaoyu Gao, Feng Liu, Gang Wang, Yu Gao, Xin Suyalatu","doi":"10.3892/etm.2023.12365","DOIUrl":"10.3892/etm.2023.12365","url":null,"abstract":"<p><p>The present study aimed to investigate the serum levels of caspase-1 in patients with high-energy pilon fractures, and its correlation with prognosis and clinical results. In this prospective study, 136 patients with high-energy pilon fractures who were treated with a locking plate combined with ankle arthroscopy from July 2015 to July 2020 were included. The treatment efficacy was evaluated according to the Mazur ankle function score. Serum caspase-1, interleukin (IL)-6, IL-1β and C-reactive protein (CRP) levels were measured using enzyme-linked immunosorbent assay. Reverse transcription-quantitative PCR was used to measure the mRNA expression of caspase-1. Additionally, demographic data and clinical characteristics, such as sex, age, intraoperative blood loss, fracture healing time, fracture classification and complications were collected and analyzed. The study revealed that the intraoperative blood loss, proportion of Ruedi-Allgower III and the serum levels of caspase-1 in the poor prognosis group were significantly higher compared with those in the good prognosis group. Additionally, patients with high-energy pilon fractures in the poor prognosis group exhibited significantly higher levels of caspase-1 and IL-1β serum levels at all time points in contrast to those in the good prognosis group. Spearman's analysis revealed a significant association between caspase-1, IL-1β levels and Mazur scores. Furthermore, caspase-1 could serve as a potential diagnostic biomarker for poor prognosis of patients with high-energy pilon fractures. Caspase-1, IL-1β, intraoperative blood loss and Ruedi-Allgower grade were the risk factors for poor prognosis in patients with high-energy pilon fractures. In summary, this study demonstrated that serum caspase-1 levels were progressively reduced during the treatment of high-energy pilon fractures patients and prominently lowered in those with a favorable prognosis. These findings could provide novel targets and a comprehensive approach to protecting patients with high-energy pilon fractures.</p>","PeriodicalId":94002,"journal":{"name":"Experimental and therapeutic medicine","volume":"27 2","pages":"77"},"PeriodicalIF":0.0,"publicationDate":"2023-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10804377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139543859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel approach for transforming breast cancer stem cells into endothelial cells","authors":"Qi-Qi Mao, Xiao-Chun Ji, Jia-Nan Zhang, Wei-Feng Teng, Shao-Cheng Zhou","doi":"10.3892/etm.2023.12362","DOIUrl":"https://doi.org/10.3892/etm.2023.12362","url":null,"abstract":"","PeriodicalId":94002,"journal":{"name":"Experimental and therapeutic medicine","volume":"19 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138950389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mimicking pneumonia with septic shock: A case report and literature review","authors":"Yufang Guo, Qiuqi Lin, Zexu Wang, Ping Zhan, Liangquan Wu, Xia Pan, Xiuwei Zhang, Li Wang, Bing Wan","doi":"10.3892/etm.2023.12361","DOIUrl":"https://doi.org/10.3892/etm.2023.12361","url":null,"abstract":"","PeriodicalId":94002,"journal":{"name":"Experimental and therapeutic medicine","volume":"117 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138953773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decrease in lipid metabolic indexes in infants with neonatal respiratory distress syndrome","authors":"Guohong Zheng, Jiansheng Zheng, Xiangrong Hu, Tang Zhu","doi":"10.3892/etm.2023.12357","DOIUrl":"https://doi.org/10.3892/etm.2023.12357","url":null,"abstract":"","PeriodicalId":94002,"journal":{"name":"Experimental and therapeutic medicine","volume":"110 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138959260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between lactate metabolism‑related molecules and venous thromboembolism: A study based on bioinformatics and an in vitro model","authors":"Zhong Qin, Jing Chen, Jianfeng Zhang, Hailin Lu, Quanzhi Chen","doi":"10.3892/etm.2023.12359","DOIUrl":"https://doi.org/10.3892/etm.2023.12359","url":null,"abstract":"","PeriodicalId":94002,"journal":{"name":"Experimental and therapeutic medicine","volume":" 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138960065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reducing polypyrimidine tract‑binding protein 1 fails to promote neuronal transdifferentiation on HT22 and mouse astrocyte cells under physiological conditions","authors":"Qian Li, Wei Zhang, Xin-yu Qiao, Chong Liu, Jian Dao, Chenmeng Qiao, Chun Cui, Yan-Qin Shen, Wei-Jiang Zhao","doi":"10.3892/etm.2023.12360","DOIUrl":"https://doi.org/10.3892/etm.2023.12360","url":null,"abstract":"","PeriodicalId":94002,"journal":{"name":"Experimental and therapeutic medicine","volume":" 47","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138962181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safranal acts as a neurorestorative agent in rats with cerebral ischemic stroke via upregulating SIRT1","authors":"Fei He, Chunmian Chen, Yangyang Wang, Shuen Wang, Shuangyan Lyu, Junqiang Jiao, Guoyong Huang, Jiangshun Yang","doi":"10.3892/etm.2023.12358","DOIUrl":"https://doi.org/10.3892/etm.2023.12358","url":null,"abstract":"","PeriodicalId":94002,"journal":{"name":"Experimental and therapeutic medicine","volume":" 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138962817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Retracted] MicroRNA‑138 suppresses proliferation, invasion and glycolysis in malignant melanoma cells by targeting HIF‑1α","authors":"Yao Chen, Ke Cao, Shao-Hua Wang, Jia Chen, Bin He, Gu He, Yong Chen, Bin Peng, Jianda Zhou","doi":"10.3892/etm.2023.12353","DOIUrl":"https://doi.org/10.3892/etm.2023.12353","url":null,"abstract":"","PeriodicalId":94002,"journal":{"name":"Experimental and therapeutic medicine","volume":"80 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138965278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}