Yin Li, Kebing Chen, Liangliang Zhu, Qing Li and Su Chen
{"title":"Microfluidic fiber-spinning chemistry for hydrophilic–hydrophobic Janus membranes towards efficient interfacial solar evaporation†","authors":"Yin Li, Kebing Chen, Liangliang Zhu, Qing Li and Su Chen","doi":"10.1039/D5NH00186B","DOIUrl":"10.1039/D5NH00186B","url":null,"abstract":"<p >Janus nanofiber membrane has emerged as a promising solar-driven interfacial evaporator for seawater desalination. However, salt ion accumulation and crystallization persist during long-term operation, which remains a key challenge. In this work, we report a hydrophilic–hydrophobic Janus nanofiber membrane evaporator, which is prepared <em>via</em> continuous microfluidic electrospinning. This method allows <em>in situ</em> chemical reaction of tannin (TA) and Fe<small><sup>3+</sup></small> to be carried out in a “Y” chip during the spinning process, enabling rapid, facile, and flexible fabrication of the nanofiber membrane. TA is rich in hydroxyl groups, which endows the bottom layer with hydrophilicity and electronegativity, thereby enhancing the water transport and Donnan effect. Thus, a high evaporation rate of 1.73 kg m<small><sup>−2</sup></small> h<small><sup>−1</sup></small> in pure water is achieved. More importantly, long-term stability in seawater desalination is realized with an evaporation rate of 1.68 kg m<small><sup>−2</sup></small> h<small><sup>−1</sup></small>, and there is no salt crystallization on the surface during continuous evaporation for 8 hours in 10 wt% NaCl solution. On the one hand, the bottom layer shows electronegativity, which is liable to immobilize cations Na<small><sup>+</sup></small> and repel anions Cl<small><sup>−</sup></small>, achieving the purpose of salt resistance. On the other hand, the Janus structure also favors the redissolution of concentrated salts into raw water, further avoiding salt accumulation. This work offers a promising common strategy for constructing high-performance Janus evaporator, which will stimulate the development of seawater desalination.</p>","PeriodicalId":93,"journal":{"name":"Nanoscale Horizons","volume":" 8","pages":" 1741-1748"},"PeriodicalIF":8.0,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Probing the spin spiral in Fe chains on Ir(001) using magnetic exchange force microscopy†","authors":"Yuuki Adachi, Yuuki Yasui, Atsushi Iiyama, Wataru Kurahashi, Rihito Nagase and Yoshiaki Sugimoto","doi":"10.1039/D5NH00162E","DOIUrl":"10.1039/D5NH00162E","url":null,"abstract":"<p >The collective motion of spin textures in atomic-scale one-dimensional systems enables information transmission with low electrical current at the nanometer scale. While reading such spin textures with current-free methods is essential for miniaturized spin-based schemes, directly probing them without relying on electrical techniques remains a significant challenge. In this study, we probed the spin texture in one-dimensional Fe chains on Ir(001) using magnetic exchange force microscopy. At large tip-sample distances, we found that ferromagnetic coupling with the tip apex magnetic atoms enables the readout of the spin texture in the Fe chain. At small tip-sample distances, we found that the spin texture in the Fe chain remained robust against chemical interactions within our measurement regime. Our ability to locally detect spins in a one-dimensional structure may pave the way for examining spin information as it propagates between the input and output of miniaturized spin logic devices.</p>","PeriodicalId":93,"journal":{"name":"Nanoscale Horizons","volume":" 8","pages":" 1653-1659"},"PeriodicalIF":8.0,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Andrey Ten, Christina Boukouvala, Vladimir Lomonosov and Emilie Ringe
{"title":"Colloidal synthesis and etching yield monodisperse plasmonic quasi-spherical Mg nanoparticles†","authors":"Andrey Ten, Christina Boukouvala, Vladimir Lomonosov and Emilie Ringe","doi":"10.1039/D5NH00205B","DOIUrl":"10.1039/D5NH00205B","url":null,"abstract":"<p >Mg is a low-cost, earth-abundant, and biocompatible plasmonic metal. Fine tuning of its optical response, required for successful light-harvesting applications, can be achieved by controlling Mg nanoparticle size and shape. Mg's hexagonal close packed crystal structure leads to the formation of a variety of unique shapes in colloidal synthesis, ranging from single crystalline hexagonal platelets to twinned rods. Yet, shape control in colloidal Mg nanoparticle synthesis is challenging due to complex nucleation and growth kinetics. Here, we present an approach to manipulate Mg nanoparticle shape by one-pot synthesis followed by colloidal etching with polycyclic aromatic hydrocarbons. We demonstrate how tips and edges in faceted Mg nanoparticles can be preferentially etched to produce quasi-spherical nanoparticles with smooth surfaces. The developed approach provides an essential shape control tool in colloidal Mg synthesis potentially applicable to other oxidising metals.</p>","PeriodicalId":93,"journal":{"name":"Nanoscale Horizons","volume":" 8","pages":" 1724-1730"},"PeriodicalIF":8.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12147706/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144245335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mauricio Cortes, Xindi Sun, Anusha, Emile Joseph Batchelder-Schwab, Jinyue Li, Naseem Siraj, Rishab Jampana, Yuchen Zhang, Yuntian Bai and Chengde Mao
{"title":"AlphaFold 3 modeling of DNA nanomotifs: is it reliable?†","authors":"Mauricio Cortes, Xindi Sun, Anusha, Emile Joseph Batchelder-Schwab, Jinyue Li, Naseem Siraj, Rishab Jampana, Yuchen Zhang, Yuntian Bai and Chengde Mao","doi":"10.1039/D5NH00059A","DOIUrl":"10.1039/D5NH00059A","url":null,"abstract":"<p >Being able to accurately predict structures is highly desirable for nanoengineering with DNA and other biomolecules. The newly launched AlphaFold 3 (AF3) provides a potential platform for this purpose. In this work, we have used AF3 to model a list of commonly used DNA nanomotifs and compared the AF3 structures with the experimentally observed structures reported in the literature. For asymmetric motifs, AF3 structures are consistent with the experimental observations; but for symmetric motifs, AF3 structures are often substantially different from experimental observations. However, the fails can be rescued if the symmetric motifs are converted into corresponding asymmetric motifs by breaking DNA sequence symmetry while maintaining the backbone symmetry. This study suggests that while AF3 is immensely helpful, we as experimentalists should use it (as it currently stands) with caution. In addition, AF3 needs further development to incorporate the existing experimental data in the training dataset for AF3. At the current stage, a hybrid approach might be beneficial: theoretical modeling softwares calculate the detailed, 3D DNA structures based on secondary DNA structures inspired by experimental observations.</p>","PeriodicalId":93,"journal":{"name":"Nanoscale Horizons","volume":" 7","pages":" 1428-1435"},"PeriodicalIF":8.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/nh/d5nh00059a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bolim You, Jihoon Huh, Yuna Kim, Mino Yang, Unjeong Kim, Min-Kyu Joo, Myung Gwan Hahm and Moonsang Lee
{"title":"Multifunctional CMOS-integrable and reconfigurable 2D ambipolar tellurene transistors for neuromorphic and in-memory computing†","authors":"Bolim You, Jihoon Huh, Yuna Kim, Mino Yang, Unjeong Kim, Min-Kyu Joo, Myung Gwan Hahm and Moonsang Lee","doi":"10.1039/D5NH00113G","DOIUrl":"10.1039/D5NH00113G","url":null,"abstract":"<p >Despite significant efforts to eliminate the von Neumann bottleneck with new two-dimensional (2D) nanomaterial-based cutting-edge device structures, there remains room for exploring alternative computing architectures that leverage 2D nanomaterials. This study introduced a groundbreaking strategy featuring a complementary metal-oxide semiconductor (CMOS)-integrable and reconfigurable ambipolar 2D tellurene (Te) transistor toward non-von Neumann computing architecture. The innovative scenario integrated seamlessly with CMOS technology, utilizing the p/n-switchable ambipolar characteristics inherited from precise Fermi-level alignment <em>via</em> thermal atomic layer deposition. Further, the architecture exhibited remarkable synaptic behavior while maintaining the conventional inverter performance within a compact single 2D Te device architecture. Expanding these findings, we demonstrated a compact programmable CMOS inverter with reduced spatial complexity and also visualized the construction of diverse complementary logic-in-memory computing. The results of this study pave the way for revolutionary in-memory computing that transcends the boundaries of the von Neumann architecture based on 2D nanomaterials.</p>","PeriodicalId":93,"journal":{"name":"Nanoscale Horizons","volume":" 8","pages":" 1760-1770"},"PeriodicalIF":8.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144172094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Verónica Mora-Sanz, Laura Saa, Valeri Pavlov, Aitziber L. Cortajarena, Bergoi Ibarlucea and Nerea Briz
{"title":"Dot-blot immunoassay based on antibody-nanocluster biohybrids as tags for naked-eye detection†","authors":"Verónica Mora-Sanz, Laura Saa, Valeri Pavlov, Aitziber L. Cortajarena, Bergoi Ibarlucea and Nerea Briz","doi":"10.1039/D5NH00045A","DOIUrl":"10.1039/D5NH00045A","url":null,"abstract":"<p >Paper-based assays such as dot-blot show high promise to develop point-of-care testing devices fulfilling the ASSURED requirements suggested by the World Health Organization (affordable, sensitive, specific, user-friendly, rapid and robust, equipment-free, and deliverable). In this technique, natural enzymes are conventionally employed tags to provide bioreceptors such as antibodies with catalytic activity for quantitative assessment. Nonetheless, their inherent biomolecular limitations pose significant challenges, including cost and storage constraints. We propose an alternative conjugation for antibodies based on catalytic bimetallic nanoclusters and their integration in such simple colorimetric paper-based immunoassay. The nanoclusters are composed of gold and platinum and they are embedded in the structure of an anti-rabbit antibody, integrating in a single component the biorecognition and transduction elements required for biosensing. The detection is based on the catalytic properties of the NCs to oxidize an insoluble chromogenic substrate, generating a visible signal on the surface of the paper that can be further analysed for quantitative results. We demonstrate the detection of antibodies against the inflammation biomarker interleukin-6 with a limit of detection of 200 ng mL<small><sup>−1</sup></small>. Experimental results reveal improvements in terms of stability compared to the natural enzyme horseradish peroxidase, retaining most of its activity after a storage equivalent to 6 months at 4 °C. Additionally, incorporating the NCs within the antibody structure instead of attaching them <em>via</em> a covalent bond provides an enhanced sensitivity of 69.7%. This assay could be transferred to other specific antibodies to detect and quantify other analytes of interest.</p>","PeriodicalId":93,"journal":{"name":"Nanoscale Horizons","volume":" 8","pages":" 1674-1683"},"PeriodicalIF":8.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sara Memarzadeh, Mateusz Gołębiewski, Maciej Krawczyk and Jarosław W. Kłos
{"title":"Nucleation and arrangement of Abrikosov vortices in hybrid superconductor–ferromagnet nanostructures†","authors":"Sara Memarzadeh, Mateusz Gołębiewski, Maciej Krawczyk and Jarosław W. Kłos","doi":"10.1039/D4NH00618F","DOIUrl":"10.1039/D4NH00618F","url":null,"abstract":"<p >This study investigates the nucleation, dynamics, and stationary configurations of Abrikosov vortices in hybrid superconductor–ferromagnet nanostructures subjected to inhomogeneous magnetic fields generated by a ferromagnetic nanodot. Employing the simulations based on time-dependent Ginzburg–Landau coupled with Maxwell's equations, we reveal the evolution of curved vortex structures that exhibit creep-like deformation before stabilizing. The interplay between vortices and currents confined within the superconducting nanoelement gives rise to unconventional stationary vortex arrangements, which evolve gradually with increasing magnetic field strength—a behavior absent in homogeneous fields. Our numerical results illustrate how the ferromagnetic element can control vortex configurations <em>via</em> a stray magnetic field—insights that are difficult to access experimentally or analytically. We demonstrate that the superconducting nanoelement can stabilize into distinct vortex states in response to even small system perturbations. This highlights the extreme sensitivity of the system and the richness of its dynamic behaviour, revealing complex pinning mechanisms and providing valuable insights into the optimisation of nanoscale superconducting systems.</p>","PeriodicalId":93,"journal":{"name":"Nanoscale Horizons","volume":" 7","pages":" 1453-1464"},"PeriodicalIF":8.0,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/nh/d4nh00618f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Priya Gupta, Harshdeep Kaur, Mohammad Anees, Sachchidanand Tiwari, Ankushi Bansal and Harpal Singh
{"title":"pH-Triggered delivery of pirarubicin–gemcitabine duo using polymeric nanoparticles for synergistic breast cancer therapy†","authors":"Priya Gupta, Harshdeep Kaur, Mohammad Anees, Sachchidanand Tiwari, Ankushi Bansal and Harpal Singh","doi":"10.1039/D4NH00654B","DOIUrl":"10.1039/D4NH00654B","url":null,"abstract":"<p >Combination chemotherapy using nanocarriers presents a promising approach to overcome the restrictions associated with conventional chemotherapy, particularly by enhancing drug stability in the bloodstream, modulating pharmacokinetics to improve therapeutic efficacy and minimizing adverse side effects on the patient's health. In pursuit of an optical treatment approach for breast cancer, various chemotherapeutic drug combinations with advanced nanocarriers are being extensively explored. This study investigated the development of pirarubicin and gemcitabine co-loaded polymeric nanoparticles for synergistic activity against breast cancer cells. To enable sustained and site-specific delivery within the tumor microenvironment, both pirarubicin and gemcitabine were chemically conjugated to a polylactic acid-based block copolymer <em>via</em> a pH-responsive “Schiff's base” linkage. The synthesized polymer–drug conjugates were subsequently formulated into Pira–Gem co-loaded block copolymeric nanoparticles, demonstrating good stability and minimal toxicity towards non-cancerous cells. Pira–Gem co-loaded nanoparticles exhibited a significantly higher percentage of drug release under acidic pH conditions, (characteristic of tumor microenvironments) compared with physiological pH conditions. Furthermore, they showed superior cellular uptake on 2D adherent cancer cell lines relative to free drugs in <em>in vitro</em> studies. Both apoptotic analysis and cell proliferation inhibition studies revealed that the co-loaded nanoparticles exhibited a synergistic therapeutic effect across multiple breast cancer cell lines, surpassing the efficacy of Pira/Gem single drug-loaded nanoparticles and their free drug counterparts. These findings suggest that the Pira–Gem co-loaded nanoformulation holds considerable promise for breast cancer therapy and requires further exploration as a potential treatment strategy.</p>","PeriodicalId":93,"journal":{"name":"Nanoscale Horizons","volume":" 7","pages":" 1465-1477"},"PeriodicalIF":8.0,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"High electrical conductivity in directionally polymerized C60 nanowires by grazing incidence of single particles†","authors":"Masaki Nobuoka, Shugo Sakaguchi, Minori Kawata, Akie Taguchi, Kosuke Kishida, Yusuke Tsutsui, Masayuki Suda, Haruka Inoue, Akira Idesaki, Tetsuya Yamaki and Shu Seki","doi":"10.1039/D5NH00228A","DOIUrl":"10.1039/D5NH00228A","url":null,"abstract":"<p >As organic electronics continue to evolve, there is a growing demand for nanometer-scale microfabrication techniques for organic semiconductors. Although precise 2D alignment and 3D integration are essential for future device applications, significant challenges remain, particularly with organic materials. Here, we demonstrate the successful fabrication of highly oriented nanowire arrays of fullerene (C<small><sub>60</sub></small>) <em>via</em> directional polymerization, mediated by grazing incidence of high-energy charged particles. These C<small><sub>60</sub></small> nanowires exhibit remarkably high electrical conductivity, comparable to that of undoped germanium, which is attributed to a unique polymerization process induced by particle irradiation. Field-effect transistor (FET) measurements revealed that electrons serve as the primary charge carriers in the nanowires. Temperature-dependent electrical measurements further indicate that the conduction mechanism follows a thermally activated hopping process, rather than conventional band conduction, reflecting the amorphous and crosslinked nature of the polymerized nanowires. Furthermore, a measurable change in conductivity upon nitrobenzene adsorption suggests their potential application as highly sensitive, electron-based organic gas sensors.</p>","PeriodicalId":93,"journal":{"name":"Nanoscale Horizons","volume":" 7","pages":" 1345-1353"},"PeriodicalIF":8.0,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/nh/d5nh00228a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144214407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pallavi Samal, Siddharth Satpathy, Lipsa Leena Panigrahi, Suman Jha and Manoranjan Arakha
{"title":"Exploring the intricacies of protein–nanoparticle interaction and its implications in chronic diseases: a comprehensive review","authors":"Pallavi Samal, Siddharth Satpathy, Lipsa Leena Panigrahi, Suman Jha and Manoranjan Arakha","doi":"10.1039/D5NH00076A","DOIUrl":"10.1039/D5NH00076A","url":null,"abstract":"<p >The protein and nanoparticle interaction is the basis of nanoparticle bio-reactivity. Nanoparticles upon interaction with proteins form a protein corona, altering their characteristics. This corona influences nanoparticles' biodistribution, pharmacokinetics, and therapeutic efficacy. The complex protein–nanoparticle interactions have a significant impact on the emergence of chronic inflammation and chronic diseases. This study is a comprehensive review that explores the dynamic nature of protein–nanoparticle interactions, emphasizing their long-term effects on sustained inflammatory responses and subsequent implications for various chronic conditions, and not an exhaustive review of all aspects. This study investigates the role of nanoparticle characteristics such as the size, shape, and surface charge in the formation of a protein corona, addressing the molecular aspects and cellular pathways involved. The connection between protein–nanoparticle interactions and chronic inflammation is deeply explored in the context of specific diseases, including cardiovascular disorders, neurological conditions, respiratory ailments, metabolic disorders, autoimmune conditions, and cancer. Insights from <em>in vivo</em> and clinical studies, coupled with discussions on genotoxicity, immunotoxicity, and mitigation strategies, contribute to a deeper understanding of the broader implications of these interactions. Nevertheless, this serves as a foundational framework for grasping the pivotal advancements and breakthroughs achieved <em>via</em> recent novel perspectives concerning the advanced methodologies for investigating protein–nanoparticle interaction and its correlation with chronic diseases. Additionally, this endeavour seeks to identify existing knowledge gaps demanding thorough exploration and offers insights for enhancing our knowledge of the interplay between protein–nanoparticle interactions and chronic disease pathogenesis. By addressing ethical considerations and public perceptions, this review outlines future research directions, highlighting the importance of extending our understanding of the safe and effective integration of nanotechnology into a broad range of applications.</p>","PeriodicalId":93,"journal":{"name":"Nanoscale Horizons","volume":" 8","pages":" 1615-1641"},"PeriodicalIF":8.0,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/nh/d5nh00076a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144232714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}