DNA and cell biology最新文献

{"title":"The Role of Regulatory Cell Death in Vitiligo.","authors":"Lyu-Ye Liu, Si-Jia He, Zhao Chen, Man Ge, Chun-Yi Lyu, Dandan Gao, Ji-Peng Yu, Meng-Han Cai, Jin-Xiang Yuan, Jun-Ling Zhang","doi":"10.1089/dna.2023.0188","DOIUrl":"10.1089/dna.2023.0188","url":null,"abstract":"<p><p>Vitiligo is one of the common chronic autoimmune skin diseases in clinic, which is characterized by localized or generalized depigmentation and seriously affects the physical and mental health of patients. At present, the pathogenesis of vitiligo is not clear; mainly, heredity, autoimmunity, oxidative stress, melanocyte (MC) self-destruction, and the destruction, death, or dysfunction of MCs caused by various reasons are always the core of vitiligo. Regulatory cell death (RCD) is an active and orderly death mode of cells regulated by genes, which widely exists in various life activities, plays a pivotal role in maintaining the homeostasis of the organism, and is closely related to the occurrence and development of many diseases. With the deepening of the research and understanding of RCD, people gradually found that there are many different forms of RCD in the lesions and perilesional skin of vitiligo patients, such as apoptosis, autophagy, pyroptosis, ferroptosis, and so on. Different cell death modes have different mechanisms in vitiligo, and different RCDs can interact and regulate each other. In this article, the mechanism related to RCD in the pathogenesis of vitiligo is reviewed, which provides new ideas for exploring the pathogenesis and targeted treatment of vitiligo.</p>","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"61-73"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139049997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oncolytic Therapy of Solid Tumors by Modified Vesicular Stomatitis Virus.","authors":"Yanhua Gao","doi":"10.1089/dna.2023.0368","DOIUrl":"10.1089/dna.2023.0368","url":null,"abstract":"<p><p>Vesicular stomatitis virus (VSV) is a promising oncolytic virus for treating solid tumors. We recently engineered a replicating VSV that specifically targets and destroys <i>Her2/neu</i>-expressing cancer cells. This virus was created by eliminating its natural binding site and adding a coding sequence for a single chain antibody to the <i>Her2/neu</i> receptor into its genome. Such an approach can be tailored to target various cellular surface molecules. This mini review will discuss genomic modifications of VSVs and their role in oncolytic therapy and discuss some challenges for moving VSVs to clinical applications.</p>","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"57-60"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138810802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomes Derived from Quercetin-Treated Bone Marrow Derived Mesenchymal Stem Cells Inhibit the Progression of Osteoarthritis Through Delivering miR-124-3p to Chondrocytes.","authors":"Shiyu Dong, Genrong Xu, Xiaoliang Li, Shengjun Guo, Jing Bai, Jiyang Zhao, Liming Chen","doi":"10.1089/dna.2023.0341","DOIUrl":"10.1089/dna.2023.0341","url":null,"abstract":"<p><p>Osteoarthritis (OA) is a chronic disease characterized by the progressive loss of cartilage and failure of the diarrheal joint. Quercetin has been reported to attenuate the development of OA. Bone marrow derived mesenchymal stem cell (BMSC)-derived exosomes are involved in OA progression. However, the role of BMSC-derived exosomes in quercetin-mediated progression of OA remains unclear. Western blotting and RT-qPCR were used to assess protein and mRNA levels, respectively. CCK8 assay was performed to assess cell viability, and cell apoptosis was assessed using flow cytometry. A dual-luciferase assay was performed to assess the relationship between miR-124-3p and TRAF6 expression. Furthermore, <i>in vivo</i> experiments were performed to test the function of exosomes derived from Quercetin-treated BMSCs in OA patients. IL-1β significantly inhibited the viability of chondrocytes, whereas the conditioned medium of Quercetin-treated BMSCs (BMSCs^QUE-CM) reversed this phenomenon through exosomes. IL-1β notably upregulated MMP13 and ADAMT5 and reduced the expression of COL2A1 in chondrocytes, which were rescued by BMSCs^QUE-CM. The effects of BMSCs^QUE-CM on these three proteins were reversed in the absence of exosomes. Exosomes can be transferred from BMSCs to chondrocytes, and exosomes derived from Quercetin-treated BMSCs (BMSCs^Que-Exo) can reverse the apoptotic effects of IL-1β on chondrocytes. The level of miR-124-3p in BMSCs was significantly upregulated by quercetin, and miR-124-3p was enriched in BMSCs^Que-Exo. TRAF6 was identified as a direct target of miR-124-3p, and BMSCs^Que-Exo abolished the IL-1β-induced activation of MAPK/p38 and NF-κB signaling. Furthermore, BMSCs^Que-Exo significantly attenuated OA progression <i>in vivo</i>. Exosomes derived from Quercetin-treated BMSCs inhibited OA progression through the upregulation of miR-124-3p.</p>","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"85-94"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139503106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation on the Effect of Fluorescence Quenching of Calf Thymus DNA by Piperine: Caspase Activation in the Human Breast Cancer Cell Line Studies.","authors":"Sakineh Rezaei, Hoda-Sadat Meftah, Yasamin Ebtehajpour, Hamid Reza Rahimi, Jamshidkhan Chamani","doi":"10.1089/dna.2023.0269","DOIUrl":"10.1089/dna.2023.0269","url":null,"abstract":"<p><p>In this study, we determined the interaction of piperine and calf thymus DNA (ct DNA) in Tris-HCl buffer solution at pH = 6.8 and also evaluated the binding mechanism through the data of multi-spectroscopic techniques along with thermal melting and viscosity measurements. The outcomes of fluorescence quenching confirmed the occurrence of interactions between piperine and ctDNA and pointed out the role of piperine as the quencher. In addition, the K_SV values were measured at three different temperatures of 298, 303, and 308 K to be 4.5 × 10⁷ M^-1, 5.65 × 10⁷ M^-1, and 9.36 × 10⁷ M^-1, respectively, which suggested the dominance of dynamic mechanism as the fluorescence quenching of piperine-ctDNA. The thermodynamic parameters revealed the predominance of hydrophobic forces in the interaction of ctDNA with piperine. According to the resonance light scattering data, the formation of a complex between piperine and ctDNA led to the creation of a larger particle. Ethidium bromide (EB) and acridine orange (AO) displacement studies, along with the ionic effects of NaCl and KI assessments, confirmed the interaction of piperine-ctDNA through a groove binding mode. The melting temperature assay of ctDNA upon the addition of piperine concentration indicated the probable groove binding of piperine to ctDNA, which was affirmed by relative viscosity measurement as well. The lack of detecting any alterations in the circular dichroism (CD) spectrum of CD investigation verified as a characteristic sign of groove binding mechanism and also confirmed all the experimental results with regard to the binding of piperine-ctDNA complex. Next to observing a concentration and time-dependent cytotoxicity in MDA-MB-231 cells, the impact of piperine on increasing lipid peroxidation and decreasing the activity of superoxide dismutase was also noticed. Apparently, piperine is capable of inducing caspase-3 activity as well.</p>","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"26-38"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138810871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and Functional Characterization of Human Sex-Determining Region on the Y Chromosome Variants Using Protamine 1 Promoter.","authors":"Deepali Pathak, Arka Baksi, S S Vasan, Rajan R Dighe","doi":"10.1089/dna.2022.0619","DOIUrl":"10.1089/dna.2022.0619","url":null,"abstract":"<p><p>The male sex-determining gene, sex-determining region on the Y chromosome (<i>SRY</i>), is expressed in adult testicular germ cells; however, its role in regulating spermatogenesis remains unclear. The role of <i>SRY</i> in the postmeiotic gene expression was investigated by determining the effect of <i>SRY</i> on the promoter of the haploid-specific Protamine 1 (<i>PRM1</i>) gene, which harbors five distinct <i>SRY</i>-binding motifs. In a luciferase reporter assay system, <i>SRY</i> upregulates <i>PRM1</i> promoter activity <i>in vitro</i> in a dose-dependent manner. Through a gel-shift assay involving a 31-bp DNA fragment encompassing the <i>SRY</i> element within the <i>PRM1</i> promoter, the third <i>SRY</i>-binding site on the sense strand (-373/-367) was identified as crucial for <i>PRM1</i> promoter activation. This assay was extended to analyze 9 <i>SRY</i> variants found in the testicular DNA of 44 azoospermia patients. The findings suggest that <i>SRY</i> regulates <i>PRM1</i> promoter activity by directly binding to its specific motif within the <i>PRM1</i> promoter.</p>","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"12-25"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139089728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Correction to:</i> Tadalafil Reverses the Effect of Three-Dimensional Cell Culture System on Stem Cell Features in A549 and SK-MES-1, by Li et al. <i>DNA and Cell Biol</i> 2021;40(7):869-880; doi: 10.1089/dna.2020.6467.","authors":"","doi":"10.1089/dna.2020.6467.correx","DOIUrl":"10.1089/dna.2020.6467.correx","url":null,"abstract":"","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"56"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138810779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening and Identifying Reference Genes for Erythrocyte Production from Cord Blood CD34+ Cells Exposed to Hypoxia.","authors":"Jun Xiao, Zhicai Li, Xiaowei Li, Huifen Lei, Fangyuan Meng, Cuiying Li","doi":"10.1089/dna.2023.0201","DOIUrl":"10.1089/dna.2023.0201","url":null,"abstract":"<p><p>Cord blood (CB) CD34+ cells have the potential to be used to achieve artificial hematopoiesis because of their ability to expand and differentiate in multiple directions. However, the mechanism and molecular changes underlying such differentiation are still unclear. The differentiation of CB CD34+ cells is generally driven by subtle changes in gene expression. A crucial method for examining gene expression is quantitative real-time polymerase chain reaction, but the accuracy of the results is dependent on the use of reliable reference genes. Here, the transcription levels of 10 novel candidate reference genes (<i>EIF4G2</i>, <i>DYNC1H1</i>, <i>LUC7L3</i>, <i>CD46</i>, <i>POLR1D</i>, <i>WSB1</i>, <i>GAPVD1</i>, <i>HGS</i>, <i>LGALS8</i>, and <i>RBM5</i>) and 8 traditional reference genes (<i>GAPDH</i>, <i>YWHAZ</i>, <i>ACTB</i>, <i>B2MG</i>, <i>TBP</i>, <i>HMBS</i>, <i>PPIA</i>, <i>HPRT1</i>) in CB CD34+ cells under different oxygen concentrations were screened and evaluated by using the geNorm and NormFinder algorithms. Comprehensive analysis conducted by RefFinder online tool showed that <i>TBP</i> (a traditional reference gene) and <i>EIF4G2</i> (a novel reference gene) had the most stable expression, whereas <i>GAPDH</i> and <i>HMBS</i> were the least suitable reference genes under these conditions. These results may serve as a basis for selecting reference genes with stable expression for more accurate normalization under different oxygen concentration stimulation during CB CD34+ cells differentiation.</p>","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138447560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circular RNA circXPO1 Promotes Multiple Myeloma Progression by Regulating miR-495-3p/DNA Damage-Induced Transcription 4 Axis.","authors":"Fangmei Li, Jing Liu, Jiyu Miao, Fei Hong, Rui Liu, Yang Lv, Yun Yang, Aili He, Jianli Wang","doi":"10.1089/dna.2023.0288","DOIUrl":"10.1089/dna.2023.0288","url":null,"abstract":"<p><p>Multiple myeloma (MM) is a hematologic malignancy that results from uncontrolled plasma cell proliferation. Circular RNAs are versatile regulators that influence cancer aggression. The pathogenic mechanism of circXPO1 in MM is still unknown. In this study, the expression of circXPO1, miR-495-3p, and DNA damage-induced transcription 4 (<i>DDIT4</i>) was detected. Knockdown and overexpression assays were used to evaluate the effect of circXPO1 on MM. Specifically, 5-ethynyl-2'-deoxyuridine and cell counting kit-8 assay were used to investigate cell proliferation. Meanwhile, flow cytometry was adopted to detect cell apoptosis and cell cycle. Apoptosis-associated and cell cycle-related proteins were detected by Western blot. Mechanistically, biotin RNA pull-down assay and dual-luciferase assay were implemented to verify the combination among miR495-3p and circXPO1 or <i>DDIT4</i>. The function of circXPO1 <i>in vivo</i> was explored in xenograft experiments. The results showed that circXPO1 was up-regulated in both MM samples and MM cell lines and miR-495-3p was down-regulated in MM patients. Silencing circXPO1 inhibited cell proliferation, increased apoptosis rates, and caused the G1 phase arrest. Overexpression of circXPO1 yielded opposite results. In addition, RNA pull-down experiment demonstrated the interaction between circXPO1 and miR-495-3p. Silencing miR-495-3p rescued the inhibitory function caused by the knockdown of circXPO1. <i>DDIT4</i> was the target of miR-495-3p. Finally, silencing circXPO1 inhibited the growth of subcutaneous tumors <i>in vivo</i>. In conclusion, our findings showed that circXPO1 could promote MM progression via the miR-495-3p/<i>DDIT4</i> axis.</p>","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"39-55"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10825292/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138810869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acknowledgment of Reviewers 2023.","authors":"","doi":"10.1089/dna.2023.29025.ack","DOIUrl":"https://doi.org/10.1089/dna.2023.29025.ack","url":null,"abstract":"","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138810788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LncRNA RP1-276N6.2 Expression and RP1-276N6.2 Gene Polymorphisms Contribute to the Risk of Coronary Artery Disease in Chinese Han Population.","authors":"Lijuan Chen, Mingming Zhao, Mingsha Zhou, Jia Luo, Shan Li, Xing Liu, Zheng Cheng, Yang Zhuo, Weiqi Zeng, Zhiyu Zhang, Li Zhou","doi":"10.1089/dna.2023.0202","DOIUrl":"10.1089/dna.2023.0202","url":null,"abstract":"<p><p>Long noncoding RNAs (lncRNAs) have been implicated in coronary artery disease (CAD) processes. However, the relationship between the gene polymorphisms of lncRNA RP1-276N6.2 as a novel molecule and susceptibility to CAD remains unclear. In our case-control study, 949 CAD patients and 892 healthy controls were genotyped using the TaqMan genotyping assay. The quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay were performed to examine the expression levels of RP1-276N6.2 and SLC22A3(OCT3). We observed that CAD patients had significantly lower RP1-276N6.2 levels than those healthy participants (<i>p</i> < 0.05). Compared to the wild-type genotype, the rs611950 T allele and the rs10499313 AG genotype and G allele significantly increased the premature CAD risk (<i>p</i> = 0.02, <i>p</i> = 0.002, and <i>p</i> = 0.01, respectively), while the rs505000 G allele reduced this risk (<i>p</i> = 0.01); moreover, the rs505000 CG genotype significantly enhanced the delayed CAD risk (<i>p</i> = 0.03), and the rs505000 G allele reduced the expression levels of RP1-276N6.2 and SLC22A3 (<i>p</i> < 0.05 and <i>p</i> < 0.05, respectively). In addition, RP1-276N6.2 positively regulated the mRNA and secreted protein levels of SLC22A3 (<i>p</i> < 0.05). In conclusion, the RP1-276N6.2 gene polymorphisms were closely associated with CAD risk. LncRNA RP1-276N6.2 may be a potential genetic target for CAD early diagnosis and treatment.</p>","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"746-752"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41242565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}