DNA and cell biology最新文献

Nucleosome Linker Length and Distribution as Major Players in Epigenetic Regulation: Insights from Cryo-Electron Microscopy and Modeling of Retina Cell Maturation. 核小体连接体长度和分布是表观遗传调控的主要参与者：来自冷冻电子显微镜和视网膜细胞成熟模型的见解。

IF 2.6

DNA and cell biology Pub Date : 2025-09-29 DOI: 10.1177/10445498251381313

Tamar Schlick, Sergei Grigoryev

引用次数: 0

m6A Modification Destabilizes Prss8 and Activates Hepatic Stellate Cells via TLR4-Mediated Inflammatory Responses. m6A修饰通过tlr4介导的炎症反应破坏Prss8的稳定性并激活肝星状细胞

IF 2.6

DNA and cell biology Pub Date : 2025-09-25 DOI: 10.1177/10445498251380337

Huimei Chen, Linhui Zhang, Lili Zhang, Tao Liu, Xue Pang, Chang Fan, Hui Jiang

{"title":"m6A Modification Destabilizes Prss8 and Activates Hepatic Stellate Cells via TLR4-Mediated Inflammatory Responses.","authors":"Huimei Chen, Linhui Zhang, Lili Zhang, Tao Liu, Xue Pang, Chang Fan, Hui Jiang","doi":"10.1177/10445498251380337","DOIUrl":"https://doi.org/10.1177/10445498251380337","url":null,"abstract":"The 6-methyladenine (m6A) modification plays a major role in various diseases. Serine protease 8 (Prss8) contributes to the initiation and progression of liver fibrosis (LF). However, the mechanism by which the m6A modification of Prss8 induces hepatic stellate cells (HSCs) activation in the LF is unclear. This study focused on exploring the contribution of Prss8 m6A modification to the pathogenesis of LF. First, primary hepatic parenchymal cells (hepatocytes) and HSCs were isolated from a mouse model of LF, and a coculture of these two types of cells was used as the object of study. Then, real-time fluorescence quantitative PCR, methylated RNA immunoprecipitation, and Western blotting were used to test the expression levels of Prss8 mRNA and protein, Prss8 m6A modification, Collagen I, α-SMA, and TLR4. Finally, the expression levels of inflammatory markers were measured via an enzyme-linked immunosorbent assay. Compared with the control group, the model group presented significantly lower Prss8 mRNA and protein levels in hepatocytes but greater levels of Prss8 m6A modification; moreover, the expression of HSC activation markers and the TLR4, IL-1β, and IL-18 proteins was significantly elevated. Mutation of the Prss8 m6A modification site led to upregulation of Prss8 mRNA and protein and decreased levels of m6A modification, TLR4, IL-1β, and IL-18. Furthermore, mutation of the Prss8 m6A modification site increased the stability of Prss8 mRNA. Rescue experiments confirmed the regulatory link between Prss8 m6A modification and TLR4. Overall, Prss8 m6A modification decreases the stability of its mRNA, promoting TLR4-mediated inflammatory cascades and leading to excessive activation of HSCs. Targeting Prss8 m6A modification is a promising therapeutic strategy for LF.","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145139818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New Insights into Host Genetic Ancestry as a Risk Factor for Dengue Disease. 宿主遗传祖先作为登革热危险因素的新见解

IF 2.6

DNA and cell biology Pub Date : 2025-09-22 DOI: 10.1177/10445498251379003

Simon M Barratt-Boyes, Priscila M S Castanha

引用次数: 0

KIAA1429 Induces the Tumorigenesis of Clear Cell Renal Cell Carcinoma via Regulating the N⁶-Methyladenosine Modification of Thymosin Beta-10. KIAA1429通过调节胸腺素β -10的n6 -甲基腺苷修饰诱导透明细胞肾细胞癌的发生。

IF 2.6

DNA and cell biology Pub Date : 2025-09-22 DOI: 10.1177/10445498251381257

Sheng Jin, Fang Liu

{"title":"KIAA1429 Induces the Tumorigenesis of Clear Cell Renal Cell Carcinoma via Regulating the N6-Methyladenosine Modification of Thymosin Beta-10.","authors":"Sheng Jin, Fang Liu","doi":"10.1177/10445498251381257","DOIUrl":"https://doi.org/10.1177/10445498251381257","url":null,"abstract":"N6-Methyladenosine (m6A) is a reversible RNA modification that regulates tumorigenesis. KIAA1429, a critical component of the m6A methyltransferase complex, has an unclear role in clear cell renal cell carcinoma (ccRCC). Here, we investigated the role of KIAA1429 in ccRCC tumorigenesis. The expressions of KIAA1429 and thymosin beta-10 (TMSB10) in ccRCC samples were evaluated using quantitative real-time PCR (qRT-PCR). The malignant features of ccRCC cells were assessed via CCK-8, colony formation, transwell migration, and invasion assays, as well as in vivo tumor xenograft models. The relationship between KIAA1429 and TMSB10 was verified via Pearson correlation analysis, methylated RNA immunoprecipitation, qRT-PCR, and Western blotting assays. Functional rescue experiments further confirmed their interaction. We found that KIAA1429 was highly expressed in ccRCC, and its silencing significantly suppressed cell proliferation, migration, invasion, and tumor growth in vivo, while overexpression had the opposite effect. Bioinformatics and mechanistic analyses identified TMSB10 as a downstream target of KIAA1429, whose expression was upregulated in an m6A-dependent manner. Furthermore, overexpressing TMSB10 partially reversed the inhibitory effects of KIAA1429 silencing on ccRCC cells. Moreover, TMSB10 overexpression partially reversed the inhibitory effects of KIAA1429 knockdown. Taken together, our findings demonstrate that KIAA1429 promotes ccRCC tumorigenesis by enhancing TMSB10 expression via m6A modification, suggesting it as a potential prognostic biomarker and therapeutic target. However, the lack of clinical validation limits the immediate translational impact of these findings.","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145115696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polymorphism of BIK as a Host Risk Factor for Severe Influenza. BIK作为严重流感宿主危险因子的多态性

IF 2.6

DNA and cell biology Pub Date : 2025-09-19 DOI: 10.1177/10445498251379681

Sourabh Soni, Yohannes A Mebratu

引用次数: 0

Retraction: Melatonin Inhibits the Proliferation of Gastric Cancer Cells Through Regulating the miR-16-5p-Smad3 Pathway. 缩回：褪黑素通过调节miR-16-5p-Smad3通路抑制胃癌细胞增殖。

IF 2.6

DNA and cell biology Pub Date : 2025-09-17 DOI: 10.1177/10445498251371637

引用次数: 0

Probiotic Feed Additive from Indigenous Bacillus subtilis Enhances Growth and Health in Common Carp (Cyprinus carpio). 原生枯草芽孢杆菌益生菌饲料添加剂促进鲤鱼生长和健康。

IF 2.6

DNA and cell biology Pub Date : 2025-09-03 DOI: 10.1177/10445498251370558

Havan Dwud Sleman, Nasreen Mohialddin Abdulrahman

{"title":"Probiotic Feed Additive from Indigenous Bacillus subtilis Enhances Growth and Health in Common Carp (Cyprinus carpio).","authors":"Havan Dwud Sleman, Nasreen Mohialddin Abdulrahman","doi":"10.1177/10445498251370558","DOIUrl":"https://doi.org/10.1177/10445498251370558","url":null,"abstract":"Probiotics sourced from host-adapted microbes represent a sustainable innovation in aquaculture nutrition, aiming to replace antibiotics and enhance fish health. However, studies evaluating indigenous probiotic strains specifically adapted to the gut environment of target species remain limited. In this study, an indigenous Bacillus subtilis strain was isolated from common carp (Cyprinus carpio) intestine, PCR-authenticated via dual endoglucanase amplicons (545 and 1311 bp), formulated as a powdered feed additive, and tested at 1, 10, and 100 mg/kg against commercial Nutri-Fish®, Infloran Bio®, vitamin C, and a supplemented control in an 8-week feeding trial with (n = 126) carp. The 10 mg/kg dose significantly (p < 0.05) enhanced growth performance, producing the highest weight gain (133.27 ± 4.56 g), specific growth rate (2.59 ± 0.12% per day), and relative growth rate (43.09 ± 1.23%), along with improved feed conversion ratio (3.51 ± 0.09%), feed efficiency ratio (29.58 ± 1.45%), and protein efficiency ratio (475.95 ± 15.32%). Hematological analysis revealed neutrophilia (52%, p < 0.05), a moderated neutrophil-to-lymphocyte ratio, and stable erythron parameters, indicating primed innate immunity without systemic stress. Plasma alanine aminotransferase and aspartate aminotransferase levels decreased by 30-40% relative to other groups (p < 0.05), evidencing improved hepatic integrity and lipid metabolism. Intestinal histology showed moderate mucosal fold hypertrophy at 10 mg/kg, whereas the highest dose (100 mg/kg) caused epithelial sloughing and inflammation. These results demonstrate that a precision microdose (10 mg/kg) of host-adapted B. subtilis can outperform multicomponent commercial supplements by significantly enhancing growth, immunity, and organ health. This probiotic strategy provides a sustainable, eco-friendly alternative for warm-water carp aquaculture, supporting circular economy principles and reducing reliance on antibiotics.","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144983858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exosome-Mediated circRNA Hsa_Circ_0113050 Enhances Colorectal Cancer Cell Malignancy by Interacting with EIF4A3. 外泌体介导的circRNA Hsa_Circ_0113050通过与EIF4A3相互作用增强结直肠癌细胞恶性。

IF 2.6

DNA and cell biology Pub Date : 2025-09-01 Epub Date: 2025-07-14 DOI: 10.1177/10445498251359374

Yuan Tian, Chen He

{"title":"Exosome-Mediated circRNA Hsa_Circ_0113050 Enhances Colorectal Cancer Cell Malignancy by Interacting with EIF4A3.","authors":"Yuan Tian, Chen He","doi":"10.1177/10445498251359374","DOIUrl":"10.1177/10445498251359374","url":null,"abstract":"The exosome-mediated circular RNAs (circRNAs) play a crucial role in tumorigenesis. The present study investigated the role of the exosome-mediated circRNA hsa_circ_0113050 in colorectal cancer (CRC) through its interaction with the eukaryotic translation initiation factor 4A3 (EIF4A3). CRC-derived exosomes were isolated and characterized by differential ultracentrifugation, transmission electron microscopy, and nanoparticle tracking analysis. The hsa_circ_0113050 expressions in CRC and exosomes were confirmed through a bioinformatic analysis and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays. Cell functional and in vivo assays were applied to evaluate the effects of exosomes and hsa_circ_0113050 on CRC cell malignancy. The interaction between EIF4A3 and hsa_circ_0113050 was analyzed by RNA immunoprecipitation, Western blotting, and qRT-PCR assays. CRC-derived exosomes with diameters of 102 and 104 nm enhanced the ability of CRC cells to proliferate, migrate, and invade. hsa_circ_0113050 was highly expressed in CRC tissues and CRC-derived exosomes. Silencing hsa_circ_0113050 in exosomes effectively reversed the exosome-induced CRC cell malignancy. Furthermore, EIF4A3 bound to the linear gene (EIF3I) of hsa_circ_0113050 to enhance the hsa_circ_0113050 expression in the CRC cells. In conclusion, the present study is the first to reveal that exosome-mediated hsa_circ_0113050 enhances CRC cell malignancy by interacting with EIF4A3. Our study findings provide new mechanistic insights into circRNA regulation and highlight a potential therapeutic target for CRC.","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"512-521"},"PeriodicalIF":2.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144628219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bile Acid-Mediated Interactions with Various Cell Types in the Cholestatic Liver. 胆汁淤积肝中胆汁酸介导的各种细胞类型的相互作用。

IF 2.6

DNA and cell biology Pub Date : 2025-09-01 Epub Date: 2025-07-15 DOI: 10.1177/10445498251359370

Guanyi He, Jie Qing

{"title":"Bile Acid-Mediated Interactions with Various Cell Types in the Cholestatic Liver.","authors":"Guanyi He, Jie Qing","doi":"10.1177/10445498251359370","DOIUrl":"10.1177/10445498251359370","url":null,"abstract":"Bile acids (BAs) have garnered significant attention due to their novel roles in modulating diverse host physiological processes. They play a crucial role in nutrient transport, organelle function, and maintaining the systemic balance of pro/anti-inflammatory states. BAs exert complex physiological effects through their interaction with nuclear receptors, such as farnesoid X receptor or cell membrane receptor Takeda G protein-coupled receptor 5. Disruption of BA transport and homeostasis results in the accumulation of BAs and elevated concentrations in the systemic circulation. This contributes to the pathogenesis of cholestatic disorders and is implicated in a variety of liver diseases, including primary biliary cholangitis and primary sclerosing cholangitis. In the context of cholestatic liver injury, BAs interact with parenchymal hepatocytes and nonparenchymal cells, leading to hepatocyte apoptosis, activation of hepatic stellate cells, and the initiation of inflammatory responses. Identifying key cellular and molecular components involved in this interaction may contribute to the development of potential therapies for cholestatic liver diseases. In this article, we provide a summary of the molecular mechanisms underlying BA-mediated interactions with various cell types in the cholestatic liver and discuss therapeutic strategies targeting BA pathways. We anticipate that a deeper understanding of these interactions will enable the formulation of novel strategies for the treatment of cholestatic liver injury.","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"502-511"},"PeriodicalIF":2.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144639018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Underpinnings of Mitochondrial Cardiomyopathy: A Scoping 2010-2024 Update. 线粒体心肌病的遗传基础：范围2010-2024更新。

IF 2.6

DNA and cell biology Pub Date : 2025-09-01 Epub Date: 2025-07-02 DOI: 10.1089/dna.2025.0089

Insaf Moudian, Joaira Bakkach, Zeineb Zian, Naima Ghailani Nourouti, Amina Barakat, Mohcine Bennani Mechita

引用次数: 0