DNA and cell biology最新文献_第2页

The Solute Carrier Family 47 Member 1, Transcriptionally Regulated by GATA Binding Protein 6, Inhibits Ferroptosis in Gastric Cancer. GATA结合蛋白6转录调控的溶质载体家族47成员1抑制胃癌铁下垂

DNA and cell biology Pub Date : 2025-07-01 Epub Date: 2025-04-28 DOI: 10.1089/dna.2025.0015

Chang'e Shi, Hezhong Yan, Qihong Zhao, Zhengli Dou, Dejie Kong, Wensheng Wang

{"title":"The Solute Carrier Family 47 Member 1, Transcriptionally Regulated by GATA Binding Protein 6, Inhibits Ferroptosis in Gastric Cancer.","authors":"Chang'e Shi, Hezhong Yan, Qihong Zhao, Zhengli Dou, Dejie Kong, Wensheng Wang","doi":"10.1089/dna.2025.0015","DOIUrl":"10.1089/dna.2025.0015","url":null,"abstract":"Gastric cancer (GC) remains the leading cause of cancer deaths worldwide. GC development is a multistep and multifactorial process, and the molecular characterization of the multistage progression of gastric lesions to GC is poorly understood. Induction of ferroptosis driven by iron-dependent phospholipid peroxidation ameliorates the malignant progression of GC. Here, we found that solute carrier family 47 member 1 (SLC47A1) promoted GC progression by regulating ferroptosis. Clinically, SLC47A1 was elevated during the progression of gastritis to GC, and its high expression was associated with poor prognosis in patients with GC. Knockdown of SLC47A1 significantly inhibited cell proliferation, colony formation, and tumor growth. Further studies revealed that SLC47A1 was a regulator of ferroptosis rather than apoptosis or necrosis. Knockdown of SLC47A1 promoted ferroptosis in GC cells, as evidenced by increased erastin-induced cytoplasmic membrane rupture, cell death, lipid peroxidation, and malondialdehyde levels. Mechanistically, GATA6 promoted SLC47A1 transcription, leading to elevated SLC47A1 expression and promoting ferroptosis in GC cells. In summary, our study revealed the significant role of SLC47A1 in the development and progression of GC through regulating ferroptosis. Targeting the GATA6/SLC47A1 axis may be a promising therapeutic strategy for GC.","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"360-369"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144047215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromatin Shearing in Suspension Cell Line: A Guide for Optimization. 悬浮细胞系染色质剪切：优化指南。

DNA and cell biology Pub Date : 2025-07-01 Epub Date: 2025-05-05 DOI: 10.1089/dna.2024.0284

Ambika Chamoli, Priyanka Patel Vatsa, Vinal Upadhyay, Amit Mandoli

{"title":"Chromatin Shearing in Suspension Cell Line: A Guide for Optimization.","authors":"Ambika Chamoli, Priyanka Patel Vatsa, Vinal Upadhyay, Amit Mandoli","doi":"10.1089/dna.2024.0284","DOIUrl":"10.1089/dna.2024.0284","url":null,"abstract":"Chromatin immunoprecipitation (ChIP) assesses DNA-proteins interactions and hence helps to generate intricate relationships and vital information. ChIP determines the genomic location of specific proteins or post-translational modifications at an individual locus or genome-wide. The protocol endures complexity; hence it is of utmost importance to identify the variable responsible for experimental erraticism. The most sensitive and critical step involves the chromatin fragmentation step. In the current study, the parameters required for chromatin shearing in the Kasumi-1 cell line have been optimized. To address this, the protocol includes the fixation of cells with formaldehyde followed by cell lysis and nuclei isolation. Further chromatin shearing using various sonication buffers and sonicator parameters was performed. Successful sonication was observed at the following settings: peak incident power of 150 W, duty factor 7.0%, cycles per burst 200, and water fill level 8 generating fragments of ∼250-600 bp in 7 min. To analyze enriched DNA sequences that are associated with the target protein ChIP coupled with quantitative PCR was performed. With this study, the optimal procedure has been standardized for a percentage of detergents, SDS (0.15%), DOC (0.05%) in the sonication buffer, and duration of sonication to achieve the desired fragmentation pattern. The quality of shearing determines the success of the experiment.","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"389-398"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144047213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Association Between MUC13 Gene Polymorphisms and Exacerbations of Asthma Under the Influence of Cigarette Smoking". “MUC13基因多态性与吸烟影响下哮喘恶化之间的关系”的勘误表。

DNA and cell biology Pub Date : 2025-07-01 Epub Date: 2025-07-02 DOI: 10.1089/dna.2024.0268.correx

引用次数: 0

Mapping Cophosphoregulation Networks Linked to Transcriptional Regulator Bromodomain-Containing Protein 4. 定位与转录调控因子含溴域蛋白4相关的磷调控网络。

DNA and cell biology Pub Date : 2025-06-12 DOI: 10.1089/dna.2025.0088

Anjana C Lalu, Fathimathul Lubaba, Athira Perunelly Gopalakrishnan, Althaf Mahin, Suhail Subair, Prathik Basthikoppa Shivamurthy, Athira C Rajeev, Rajesh Raju

{"title":"Mapping Cophosphoregulation Networks Linked to Transcriptional Regulator Bromodomain-Containing Protein 4.","authors":"Anjana C Lalu, Fathimathul Lubaba, Athira Perunelly Gopalakrishnan, Althaf Mahin, Suhail Subair, Prathik Basthikoppa Shivamurthy, Athira C Rajeev, Rajesh Raju","doi":"10.1089/dna.2025.0088","DOIUrl":"https://doi.org/10.1089/dna.2025.0088","url":null,"abstract":"Bromodomain-containing protein 4 (BRD4) is a pivotal transcriptional regulator implicated in cancer, fibrosis, and inflammation, yet its phospho-regulatory network remains underexplored. This study leverages an extensive analysis of 1000 qualitative and 225 quantitative global phosphoproteome datasets to decode the BRD4 phosphorylation landscape. We identified S601 and S1117 as predominant phosphorylation sites, driving the majority of BRD4 phospho-signaling. Co-regulation analysis revealed 755 and 972 proteins positively cophosphorylated with S601 and S1117, respectively, including key interactors like TRIM28 (S473) and PRKAR2A (S78), which enhance transcriptional activity and cAMP signaling. Upstream kinases MAPK14 and GRK5 emerged as high-confidence regulators of S1117 and S601, respectively, with correlations in breast cancer highlighting disease relevance. In addition, 93 phosphosites in 71 transcription factors co-regulated with S1117 and 69 in 53 with S601 underscore the role of BRD4 in transcription control. These findings unveil a complex phospho-signaling network, offering novel therapeutic targets for BRD4-associated diseases and a foundation for future experimental validation.","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144277127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

UBE2C, Regulated by n6-methyladenosine Methyltransferase METTL3, Is an Oncogene in Retinoblastoma via PI3K-AKT Pathway. n6-甲基腺苷甲基转移酶METTL3调控的UBE2C是一个通过PI3K-AKT通路在视网膜母细胞瘤中的致癌基因。

DNA and cell biology Pub Date : 2025-06-04 DOI: 10.1089/dna.2025.0074

Lan Chen, Songhua Mei

{"title":"UBE2C, Regulated by n6-methyladenosine Methyltransferase METTL3, Is an Oncogene in Retinoblastoma via PI3K-AKT Pathway.","authors":"Lan Chen, Songhua Mei","doi":"10.1089/dna.2025.0074","DOIUrl":"https://doi.org/10.1089/dna.2025.0074","url":null,"abstract":"Retinoblastoma (RB) arising from the retina's primitive neural precursor cells is a highly aggressive pediatric ocular malignancy. Ubiquitin-conjugating enzyme E2C (UBE2C) is implicated in carcinogenesis, but its role and mechanism in RB remain unexplored. Here, we aimed to explore the effect of UBE2C and its regulatory mechanism in an N6-methyladenosine (m6A) modification method in RB. The expression of UBE2C and methyltransferase-like 3 (METTL3) was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blotting. After using shRNA and overexpression vectors to modulate the expression of UBE2C and METTL3 in RB cells, cell viability, proliferation, apoptosis, and phosphoinositide 3-kinase-protein kinase B (PI3K-AKT) pathway activity were assessed via cell counting kit-8, 5-ethynyl-2'-deoxyuridine, flow cytometry, and Western blotting assays, respectively. The correlation between METTL3 and UBE2C in RB cells was verified by qRT-PCR, Western blotting, methylated RNA immunoprecipitation, mRNA stability assays. The results showed that UBE2C with high expression in RB enhanced RB cell survival via elevating cell viability and proliferation, as well as suppressing apoptosis. UBE2C activated the PI3K-AKT pathway by promoting the PI3K and AKT proteins. METTL3 upregulated UBE2C expression and enhanced UBE2C mRNA stability via m6A modification. In addition, upregulating METTL3 partly restored the negative effects of UBE2C downregulation on RB cells. In conclusion, METTL3 drives UBE2C overexpression through m6A modification, thereby activating the PI3K-AKT pathway to foster RB progression. This study identifies the METTL3/UBE2C/PI3K-AKT axis as a novel therapeutic target for RB.","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Therapeutic Potential of Extracellular Vesicles in Sepsis Management. 细胞外囊泡在脓毒症治疗中的治疗潜力。

DNA and cell biology Pub Date : 2025-06-01 Epub Date: 2025-05-05 DOI: 10.1089/dna.2025.0009

Laifa Kong, Qigang Huang, Jianghua Cheng, Yingwei Ding, Baodi Wang

引用次数: 0

Mechanism of LncRNA-MiRNA in Renal Intrinsic Cells of Diabetic Kidney Disease and Potential Therapeutic Direction. 糖尿病肾病肾固有细胞中 LncRNA-MiRNA 的作用机制及潜在治疗方向

DNA and cell biology Pub Date : 2025-06-01 Epub Date: 2025-03-21 DOI: 10.1089/dna.2025.0026

Xiyue Tian, Min Zhou, Jingbo Zhang, Xinchun Huang, Dongyang Jiang, Jian Liu, Qiong Zhang, Dingguo Chen, Qiongdan Hu

{"title":"Mechanism of LncRNA-MiRNA in Renal Intrinsic Cells of Diabetic Kidney Disease and Potential Therapeutic Direction.","authors":"Xiyue Tian, Min Zhou, Jingbo Zhang, Xinchun Huang, Dongyang Jiang, Jian Liu, Qiong Zhang, Dingguo Chen, Qiongdan Hu","doi":"10.1089/dna.2025.0026","DOIUrl":"10.1089/dna.2025.0026","url":null,"abstract":"The occurrence of diabetic kidney disease (DKD), a critical microvascular issue in diabetes, is progressively on the rise. In recent years, long noncoding RNAs (lncRNAs) have garnered considerable attention as a novel and critical layer of biological regulation. Our knowledge regarding the roles and underlying mechanisms of lncRNAs in various diseases, including DKD, continues to evolve. Similarly, microRNAs (miRNAs), which are small noncoding RNAs, have been recognized as crucial contributors to cellular processes and disease pathogenesis. Emerging studies have highlighted the complex interactions between lncRNAs and miRNAs, particularly in the context of DKD, underscoring their importance in complex human diseases. Renal intrinsic cell damage is an important cause of inducing DKD. Persistent high glucose stimulation leads to remodeling of renal intrinsic cells and a cascade of pathological changes. This article aims to review recent literature on the lncRNAs-mediated regulation of miRNAs affecting renal intrinsic cells in DKD and to propose novel molecular-level therapeutic strategies for DKD. Through in-depth investigation of this dynamic molecular interaction, we can gain a profound understanding of the potential mechanisms underlying diabetic nephropathy, potentially identifying new targets for therapeutic intervention and paving the way for personalized and effective treatments.","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"304-324"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143675119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Guardians of the Lung: The Multifaceted Roles of Macrophages in Cancer and Infectious Disease. 肺的守护者：巨噬细胞在癌症和传染病中的多方面作用。

DNA and cell biology Pub Date : 2025-06-01 Epub Date: 2025-03-19 DOI: 10.1089/dna.2024.0211

Zhi Liu, Yangjing Ou, Xiaojin He, Ting Yuan, Miao Li, Yunzhu Long, Yukun Li, Yingzheng Tan

{"title":"Guardians of the Lung: The Multifaceted Roles of Macrophages in Cancer and Infectious Disease.","authors":"Zhi Liu, Yangjing Ou, Xiaojin He, Ting Yuan, Miao Li, Yunzhu Long, Yukun Li, Yingzheng Tan","doi":"10.1089/dna.2024.0211","DOIUrl":"10.1089/dna.2024.0211","url":null,"abstract":"The lung as an organ that is fully exposed to the external environment for extended periods, comes into contact with numerous inhaled microorganisms. Lung macrophages are crucial for maintaining lung immunity and operate primarily through signaling pathways such as toll-like receptor 4 and nuclear factor-κB pathways. These macrophages constitute a diverse population with significant plasticity, exhibiting different phenotypes and functions on the basis of their origin, tissue residence, and environmental factors. During lung homeostasis, they are involved in the clearance of inhaled particles, cellular remnants, and even participate in metabolic processes. In disease states, lung macrophages transition from the inflammatory M1 phenotype to the anti-inflammatory M2 phenotype. These distinct phenotypes have varying transcriptional profiles and serve different functions, from combating pathogens to repairing inflammation-induced damage. However, macrophages can also exacerbate lung injury during prolonged inflammation or exposure to antigens. In this review, we delve into the diverse roles of pulmonary macrophages the realms in homeostasis, pneumonia, tuberculosis, and lung tumors.","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"249-262"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143665443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crosstalk Between Plk1 and PTEN in Mitosis Affects Chromosomal Stability. 有丝分裂中Plk1和PTEN间的串扰影响染色体稳定性。

DNA and cell biology Pub Date : 2025-06-01 Epub Date: 2025-03-21 DOI: 10.1089/dna.2024.0246

Wei Li, Xianning Wang, Jiannan Liu, Bing Liu, Yongjian Hao

{"title":"Crosstalk Between Plk1 and PTEN in Mitosis Affects Chromosomal Stability.","authors":"Wei Li, Xianning Wang, Jiannan Liu, Bing Liu, Yongjian Hao","doi":"10.1089/dna.2024.0246","DOIUrl":"10.1089/dna.2024.0246","url":null,"abstract":"The mitotic phase involves the distribution and regulation of genetic material. Defects in gene regulation can lead to serious errors in genetic transmission, such as increased instability of chromosomes, thereby increasing susceptibility to cancer and promoting its development. The maintenance of chromosome stability depends on several mechanisms, such as efficient DNA repair, proper sister chromatid separation, and timely cytokinesis. The serine/threonine kinase Plk1 is a key molecule in maintaining chromosome stability, participating in multiple stages of precise regulation during mitosis, including promoting entry into mitosis, facilitating centrosome maturation and bipolar spindle formation, promoting sister chromatid separation, and facilitating cytokinesis. Several proteins can regulate the kinase activity of Plk1 through protein-protein interactions, coordinating the genetic stability of the cell, including the kinases Aurora A, c-Abl, and Chk1 as well as the phosphatase phosphatase and tension homolog (PTEN). PTEN has been described as an essential regulator of Plk1 for dephosphorylation and chromosomal stability during cell division, and Plk1 may directly interact with and phosphorylate PTEN at centromeres. Here, we review the bidirectional interplay between Plk1 and PTEN and how it contributes to genomic stability during mitosis.","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"263-273"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143675056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and Chromosomal Features of Guizhou Wangmo Babu Tea. 贵州望谟八步茶的形态与染色体特征。

DNA and cell biology Pub Date : 2025-06-01 Epub Date: 2025-04-28 DOI: 10.1089/dna.2024.0290

Ying Wang, Shu Wang, Leijia Li, Cheng Wan, Quanshu Luo, JiHong Wang

{"title":"Morphological and Chromosomal Features of Guizhou Wangmo Babu Tea.","authors":"Ying Wang, Shu Wang, Leijia Li, Cheng Wan, Quanshu Luo, JiHong Wang","doi":"10.1089/dna.2024.0290","DOIUrl":"10.1089/dna.2024.0290","url":null,"abstract":"The taxonomic classification of Babu tea (Family Theaceae) was preliminarily investigated by analyzing its epigenetic morphology and chromosomal features. Morphological observations of three Babu Tea phenotypes (red flower/purple bud, pink flower/purple bud, and white flower/green bud) revealed that the stomata were located exclusively on the lower epidermis, and the anticlinal walls of epidermal cells were deeply undulated. The stomatal apparatus also consisted of two crescent-shaped guard cells, forming a spindle shape with thickened \"T-shaped\" ends. Furthermore, the outer stomatal arches had smooth surfaces, while the inner ones were shallowly undulated. Finally, subsidiary cells were covered by a shallowly undulated epidermal stratum corneum that was composed of three layers. Their pollen grains exhibited a prolate spherical shape. Based on pollen morphology, the Babu Tea was therefore classified within Sect. Theopsis Coh. St., Camellia. Chromosome counting and karyotype analysis further confirmed that the three phenotypes share a chromosome count of 2n = 2x = 30, with their karyotypes classified as Type 2A. These findings suggest that the three phenotypes share a common evolutionary origin.","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"284-293"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0