DNA and cell biology最新文献_第10页

IFI16 Positively Regulates RIG-I-Mediated Type I Interferon Production in a STING-Independent Manner. IFI16 以 STING 依赖性方式积极调节 RIG-I 介导的 I 型干扰素的产生。

DNA and cell biology Pub Date : 2024-04-01 Epub Date: 2024-03-11 DOI: 10.1089/dna.2023.0362

Xibao Shi, Menglu Wei, Yuwen Feng, Yuanhao Yang, Xiaozhuan Zhang, Hao Chen, Yuqi Xing, Keqi Wang, Wensheng Wang, Li Wang, Aiping Wang, Gaiping Zhang

{"title":"IFI16 Positively Regulates RIG-I-Mediated Type I Interferon Production in a STING-Independent Manner.","authors":"Xibao Shi, Menglu Wei, Yuwen Feng, Yuanhao Yang, Xiaozhuan Zhang, Hao Chen, Yuqi Xing, Keqi Wang, Wensheng Wang, Li Wang, Aiping Wang, Gaiping Zhang","doi":"10.1089/dna.2023.0362","DOIUrl":"10.1089/dna.2023.0362","url":null,"abstract":"Previous studies have shown that interferon gene-stimulating protein (STING) is essential for IFN-γ-inducible protein 16 (IFI16) as the DNA sensor and RNA sensor to induce transcription of type I interferon (IFN-I) and is essential for IFI16 to synergize with DNA sensor GMP-AMP (cGAMP) synthase (cGAS) in induction of IFN-I transcription. While other and our previous studies have shown that IFI16 enhanced retinoic acid-inducible gene I (RIG-I)-, which was an RNA sensor, and mitochondrial antiviral signaling (MAVS)-, which was the adaptor protein of RIG-I, induced production of IFN-I, so we wonder whether IFI16 regulates the signal pathway of RNA-RIG-I-MAVS-IFN-I in a STING-dependent manner. We used HEK 293T cells, which did not express endogenous STING and were unable to mount an innate immune response upon DNA transfection and found that IFI16 could enhance RIG-I- and MAVS-mediated induction of IFN-I in a STING-independent way. Furthermore, we found that upregulation of the expression of NF-kappa-B essential modulator (NEMO) by IFI16 was not the mechanism that IFI16 regulated the induction of IFN-I. In conclusion, we found that IFI16 regulated the signal pathway of RNA-RIG-I-MAVS-IFN-I in a STING-independent manner.","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"197-205"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140103023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role Played by Mitochondria in Polycystic Ovary Syndrome. 线粒体在多囊卵巢综合征中的作用

DNA and cell biology Pub Date : 2024-04-01 DOI: 10.1089/dna.2023.0345

Chang Sun, Shanshan Zhao, Zimeng Pan, Jing Li, Yasong Wang, Hongying Kuang

引用次数: 0

Tanshinone I Stimulates Pyroptosis of Cisplatin-Resistant Gastric Cancer Cells by Activating the NF-κB/Caspase-3(8)/GSDME Signaling Pathway. 丹参酮 I 通过激活 NF-κB/Caspase-3(8)/GSDME 信号通路刺激顺铂耐药胃癌细胞的嗜热反应

DNA and cell biology Pub Date : 2024-04-01 Epub Date: 2024-03-11 DOI: 10.1089/dna.2023.0293

Guijun Wang, Yanrong Li, Zhaokai Guo, Qiang He, Zhen Liu, Beibei Deng

{"title":"Tanshinone I Stimulates Pyroptosis of Cisplatin-Resistant Gastric Cancer Cells by Activating the NF-κB/Caspase-3(8)/GSDME Signaling Pathway.","authors":"Guijun Wang, Yanrong Li, Zhaokai Guo, Qiang He, Zhen Liu, Beibei Deng","doi":"10.1089/dna.2023.0293","DOIUrl":"10.1089/dna.2023.0293","url":null,"abstract":"Cisplatin (DDP) resistance frequently occurs in gastric cancer (GC) therapy. Tanshinone I is a liposoluble phenanthraquinone compound present in the roots of Salvia miltiorrhiza Bunge (Danshen). In this study, we aimed to explore the effects of tanshinone I on modulating DDP resistance of GC cells in vitro and in vivo. DDP-resistant GC cell models (BGC823/DDP and SGC7901/DDP) were established, and their viability, proliferation, migration, lactate dehydrogenase activity, reactive oxygen species (ROS) generation, and pyroptosis were assessed after DDP treatment with or without tanshinone I. In addition, a mouse model with subcutaneously transplanted GC tumors was established to confirm the effects of tanshinone I and DDP on tumor growth and cell pyroptosis. The results revealed that tanshinone I inhibited DDP-resistant GC cell proliferation and migration; increased intracellular ROS levels; and activated cell pyroptosis by enhancing the levels of cleaved caspase-8, cleaved caspase-3, GSDME-NT, phospho-IKK-α/β, and nuclear factor kappa-B (NF-κB). GSDME knockdown weakened these effects of tanshinone I on DDP-resistant GC cells. Furthermore, DDP combined with tanshinone I inhibited the growth of subcutaneously transplanted GC tumors in mice by reducing cell proliferation and inducing pyroptosis. In conclusion, tanshinone I reversed DDP resistance of GC cells by stimulating pyroptosis, by activating NF-κB/caspase-3(8)/GSDME signaling pathway.","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"185-196"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140103024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of Complement Component C3 in Protection Against Pseudomonas Pneumonia-Induced Lung Injury. 补体成分 C3 在保护肺部免受假单胞菌肺炎引起的肺损伤中的作用

DNA and cell biology Pub Date : 2024-04-01 Epub Date: 2024-02-06 DOI: 10.1089/dna.2023.0445

Sanjaya K Sahu, Rahul K Maurya, Hrishikesh S Kulkarni

{"title":"The Role of Complement Component C3 in Protection Against Pseudomonas Pneumonia-Induced Lung Injury.","authors":"Sanjaya K Sahu, Rahul K Maurya, Hrishikesh S Kulkarni","doi":"10.1089/dna.2023.0445","DOIUrl":"10.1089/dna.2023.0445","url":null,"abstract":"The complement system is a family of proteins that facilitate immune resistance by attacking microbes to decrease pathogen burden. As a result, deficiencies of certain complement proteins result in recurrent bacterial infections, and can also result in acute lung injury (ALI). We and others have shown that C3 is present in both immune and nonimmune cells, and modulates cellular functions such as metabolism, differentiation, cytokine production, and survival. Although the emerging roles of the complement system have implications for host responses to ALI, key questions remain vis-a-vis the lung epithelium. In this review, we summarize our recent article in which we reported that during Pseudomonas aeruginosa-induced ALI, lung epithelial cell-derived C3 operates independent of liver-derived C3. Specifically, we report the use of a combination of human cell culture systems and global as well as conditional knockout mouse models to demonstrate the centrality of lung epithelial cell-derived C3. We also summarize recent articles that have interrogated the role of intracellular and/or locally derived C3 in host defense. We propose that C3 is a highly attractive candidate for enhancing tissue resilience in lung injury as it facilitates the survival and function of the lung epithelium, a key cell type that promotes barrier function.","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"153-157"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11002327/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139699075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA and cell biology Pub Date : 2024-04-01 Epub Date: 2024-03-11 DOI: 10.1089/dna.2023.0392

Yun Bai, Guanghua Cui, Xiaoke Sun, Meiqi Wei, Yanying Liu, Jialu Guo, Yu Yang

{"title":"Angiopoietin-Related Protein 4-Transcript 3 Increases the Proliferation, Invasion, and Migration of Hepatocellular Carcinoma Cells and Inhibits Apoptosis.","authors":"Yun Bai, Guanghua Cui, Xiaoke Sun, Meiqi Wei, Yanying Liu, Jialu Guo, Yu Yang","doi":"10.1089/dna.2023.0392","DOIUrl":"10.1089/dna.2023.0392","url":null,"abstract":"To investigate the functional differences of angiopoietin-related protein 4 (ANGPTL4) transcripts in hepatocellular carcinoma (HCC) cells. By transfecting ANGPTL4-Transcript 1 and ANGPTL4-Transcript 3 overexpression vectors into HepG2 and Huh7 cell lines with ANGPTL4 knockdown, the effects of overexpression of two transcripts on cell viability, invasion, migration, and apoptosis were analyzed. The expression of two transcripts was compared in human liver cancer tissue, and their effects on tumor development were validated in vivo experiments in mice. Compared with control, the overexpression of ANGPTL4-Transcript 1 had no significant effect on viability, invasion, healing, and apoptosis of HepG2 and Huh7 cells. However, these two cell lines overexpressing ANGPTL4-Transcript 3 showed remarkably enhanced cell viability, invasive and healing ability, and decreased apoptosis ability. Furthermore, the mRNA level of ANGPTL4-Transcript 3 was significantly increased in human HCC tissues and promoted tumor growth compared with Transcript 1. Different transcripts of gene ANGPTL4 have distinct effects on HCC. The abnormally elevated Transcript 3 with the specific ability of promoting HCC proliferation, infiltration, and migration is expected to become a new biological marker and more precise intervention target for HCC.","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"175-184"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140103022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SHCBP1 Overexpression Aggravates Pancreatitis by Triggering the Loss of Primary Cilia. SHCBP1 过度表达会引发原发性纤毛缺失，从而加重胰腺炎。

DNA and cell biology Pub Date : 2024-03-01 Epub Date: 2024-01-12 DOI: 10.1089/dna.2023.0240

Lianshun Li, Huiming Zhao, Zhengyang Li, Wengui Shi, Zuoyi Jiao

引用次数: 0

Mechanisms of Plasmid Behavioral Manipulation. 致编辑的信：质粒行为操纵机制。

DNA and cell biology Pub Date : 2024-03-01 Epub Date: 2024-01-30 DOI: 10.1089/dna.2023.0402

Jacob G Malone, Catriona M A Thompson

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MicroRNAs in Male Fertility. 男性生育能力中的微RNA。

DNA and cell biology Pub Date : 2024-03-01 Epub Date: 2024-02-23 DOI: 10.1089/dna.2023.0314

Sedigheh Bahmyari, Seyyed Hossein Khatami, Sina Taghvimi, Sahar Rezaei Arablouydareh, Mortaza Taheri-Anganeh, Hojat Ghasemnejad-Berenji, Tooba Farazmand, Elahe Soltani Fard, Arezoo Solati, Ahmad Movahedpour, Hassan Ghasemi

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Aspirin Challenge-Induced Genome-Wide DNA Methylation Profile of Peripheral Blood Lymphocytes in Aspirin-Exacerbated Respiratory Disease. 阿司匹林加重呼吸道疾病患者外周血淋巴细胞中阿司匹林挑战诱导的全基因组DNA甲基化谱图

DNA and cell biology Pub Date : 2024-03-01 Epub Date: 2024-02-22 DOI: 10.1089/dna.2023.0218

Jong-Uk Lee, Hun Soo Chang, Ji-Su Shim, Min-Hye Kim, Young-Joo Cho, Min Kyung Kim, Seung-Lee Park, Sun Ju Lee, Jong-Sook Park, Choon-Sik Park

{"title":"Aspirin Challenge-Induced Genome-Wide DNA Methylation Profile of Peripheral Blood Lymphocytes in Aspirin-Exacerbated Respiratory Disease.","authors":"Jong-Uk Lee, Hun Soo Chang, Ji-Su Shim, Min-Hye Kim, Young-Joo Cho, Min Kyung Kim, Seung-Lee Park, Sun Ju Lee, Jong-Sook Park, Choon-Sik Park","doi":"10.1089/dna.2023.0218","DOIUrl":"10.1089/dna.2023.0218","url":null,"abstract":"Genetic variation and epigenetic factors are thought to contribute to the development of hypersensitivity to aspirin. DNA methylation fluctuates dynamically throughout the day. To discover new CpG methylation in lymphocytes associated with aspirin-exacerbated respiratory disease (AERD), we evaluated changes in global CpG methylation profiles from before to after an oral aspirin challenge in patients with AERD and aspirin-tolerant asthma (ATA). Whole-genome CpG methylation levels of peripheral blood mononuclear cells were quantified with an Illumina 860K Infinium Methylation EPIC BeadChip array and then adjusted for inferred lymphocyte fraction (ILF) with GLINT and Tensor Composition Analysis. Among the 866,091 CpGs in the array, differentially methylated CpGs (DMCs) were found in 6 CpGs in samples from all 12 patients with asthma included in the study (AERD, n = 6; ATA, n = 6). DMCs were found in 3 CpGs in the 6 ATA samples and in 615 CpGs in the 6 AERD samples. A total of 663 DMCs in 415 genes and 214 intergenic regions differed significantly in the AERD compared with the ATA. In promoters, 126 CpG loci were predicted to bind to 38 transcription factors (TFs), many of which were factors already known to be involved in the pathogenesis of asthma and immune responses. In conclusion, we identified 615 new CpGs methylated in peripheral blood lymphocytes by oral aspirin challenge in AERD but not in ATA. These findings indicate that oral aspirin challenge induces epigenetic changes in ILFs, specifically in AERD patients, possibly via changes in TF binding, which may have epigenetic effects on the development of AERD.","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"132-140"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139934711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloning and Expression Analysis of Key Enzyme Gene CoGPPS Involved in Iridoid Glycoside Synthesis in Cornus officinalis. 参与山茱萸铱苷合成的关键酶基因 CoGPPS 的克隆和表达分析

DNA and cell biology Pub Date : 2024-03-01 Epub Date: 2024-02-13 DOI: 10.1089/dna.2023.0335

Jiaxi Chen, Xinjie Tan, Guangyang Guo, Panpan Wang, Hongxiao Zhang, Shufang Lv, Huawei Xu, Dianyun Hou

{"title":"Cloning and Expression Analysis of Key Enzyme Gene CoGPPS Involved in Iridoid Glycoside Synthesis in Cornus officinalis.","authors":"Jiaxi Chen, Xinjie Tan, Guangyang Guo, Panpan Wang, Hongxiao Zhang, Shufang Lv, Huawei Xu, Dianyun Hou","doi":"10.1089/dna.2023.0335","DOIUrl":"10.1089/dna.2023.0335","url":null,"abstract":"Cornus iridoid glycosides (CIGs), including loganin and morroniside, are the main active components of Cornus officinalis. As one of the key enzymes in the biosynthesis of CIGs, geranyl pyrophosphate synthase (GPPS) catalyzes the formation of geranyl pyrophosphate, which is the direct precursor of CIGs. In this study, the C. officinalis geranyl pyrophosphate synthase (CoGPPS) sequence was cloned from C. officinalis and analyzed. The cDNA sequence of the CoGPPS gene was 915 bp (GenBank No. OR725699). Phylogenetic analysis showed that CoGPPS was closely related to the GPPS sequence of Actinidia chinensis and Camellia sinensis, but relatively distantly related to Paeonia lactiflora and Tripterygium wilfordii. Results from the quantitative real-time PCR showed the spatiotemporal expression pattern of CoGPPS; that is, CoGPPS was specifically expressed in the fruits. Subcellular localization assay proved that CoGPPS was specifically found in chloroplasts. Loganin and morroniside contents in the tissues were detected by high-performance liquid chromatography, and both compounds were found to be at higher levels in the fruits than in leaves. Thus, this study laid the foundation for further studies on the synthetic pathway of CIGs.","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"125-131"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139731278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0