DNA and cell biology最新文献_第8页

The Role of Complement Component C3 in Protection Against Pseudomonas Pneumonia-Induced Lung Injury. 补体成分 C3 在保护肺部免受假单胞菌肺炎引起的肺损伤中的作用

DNA and cell biology Pub Date : 2024-04-01 Epub Date: 2024-02-06 DOI: 10.1089/dna.2023.0445

Sanjaya K Sahu, Rahul K Maurya, Hrishikesh S Kulkarni

{"title":"The Role of Complement Component C3 in Protection Against Pseudomonas Pneumonia-Induced Lung Injury.","authors":"Sanjaya K Sahu, Rahul K Maurya, Hrishikesh S Kulkarni","doi":"10.1089/dna.2023.0445","DOIUrl":"10.1089/dna.2023.0445","url":null,"abstract":"The complement system is a family of proteins that facilitate immune resistance by attacking microbes to decrease pathogen burden. As a result, deficiencies of certain complement proteins result in recurrent bacterial infections, and can also result in acute lung injury (ALI). We and others have shown that C3 is present in both immune and nonimmune cells, and modulates cellular functions such as metabolism, differentiation, cytokine production, and survival. Although the emerging roles of the complement system have implications for host responses to ALI, key questions remain vis-a-vis the lung epithelium. In this review, we summarize our recent article in which we reported that during Pseudomonas aeruginosa-induced ALI, lung epithelial cell-derived C3 operates independent of liver-derived C3. Specifically, we report the use of a combination of human cell culture systems and global as well as conditional knockout mouse models to demonstrate the centrality of lung epithelial cell-derived C3. We also summarize recent articles that have interrogated the role of intracellular and/or locally derived C3 in host defense. We propose that C3 is a highly attractive candidate for enhancing tissue resilience in lung injury as it facilitates the survival and function of the lung epithelium, a key cell type that promotes barrier function.","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"153-157"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11002327/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139699075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA and cell biology Pub Date : 2024-04-01 Epub Date: 2024-03-11 DOI: 10.1089/dna.2023.0392

Yun Bai, Guanghua Cui, Xiaoke Sun, Meiqi Wei, Yanying Liu, Jialu Guo, Yu Yang

{"title":"Angiopoietin-Related Protein 4-Transcript 3 Increases the Proliferation, Invasion, and Migration of Hepatocellular Carcinoma Cells and Inhibits Apoptosis.","authors":"Yun Bai, Guanghua Cui, Xiaoke Sun, Meiqi Wei, Yanying Liu, Jialu Guo, Yu Yang","doi":"10.1089/dna.2023.0392","DOIUrl":"10.1089/dna.2023.0392","url":null,"abstract":"To investigate the functional differences of angiopoietin-related protein 4 (ANGPTL4) transcripts in hepatocellular carcinoma (HCC) cells. By transfecting ANGPTL4-Transcript 1 and ANGPTL4-Transcript 3 overexpression vectors into HepG2 and Huh7 cell lines with ANGPTL4 knockdown, the effects of overexpression of two transcripts on cell viability, invasion, migration, and apoptosis were analyzed. The expression of two transcripts was compared in human liver cancer tissue, and their effects on tumor development were validated in vivo experiments in mice. Compared with control, the overexpression of ANGPTL4-Transcript 1 had no significant effect on viability, invasion, healing, and apoptosis of HepG2 and Huh7 cells. However, these two cell lines overexpressing ANGPTL4-Transcript 3 showed remarkably enhanced cell viability, invasive and healing ability, and decreased apoptosis ability. Furthermore, the mRNA level of ANGPTL4-Transcript 3 was significantly increased in human HCC tissues and promoted tumor growth compared with Transcript 1. Different transcripts of gene ANGPTL4 have distinct effects on HCC. The abnormally elevated Transcript 3 with the specific ability of promoting HCC proliferation, infiltration, and migration is expected to become a new biological marker and more precise intervention target for HCC.","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"175-184"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140103022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SHCBP1 Overexpression Aggravates Pancreatitis by Triggering the Loss of Primary Cilia. SHCBP1 过度表达会引发原发性纤毛缺失，从而加重胰腺炎。

DNA and cell biology Pub Date : 2024-03-01 Epub Date: 2024-01-12 DOI: 10.1089/dna.2023.0240

Lianshun Li, Huiming Zhao, Zhengyang Li, Wengui Shi, Zuoyi Jiao

引用次数: 0

Mechanisms of Plasmid Behavioral Manipulation. 致编辑的信：质粒行为操纵机制。

DNA and cell biology Pub Date : 2024-03-01 Epub Date: 2024-01-30 DOI: 10.1089/dna.2023.0402

Jacob G Malone, Catriona M A Thompson

引用次数: 0

MicroRNAs in Male Fertility. 男性生育能力中的微RNA。

DNA and cell biology Pub Date : 2024-03-01 Epub Date: 2024-02-23 DOI: 10.1089/dna.2023.0314

Sedigheh Bahmyari, Seyyed Hossein Khatami, Sina Taghvimi, Sahar Rezaei Arablouydareh, Mortaza Taheri-Anganeh, Hojat Ghasemnejad-Berenji, Tooba Farazmand, Elahe Soltani Fard, Arezoo Solati, Ahmad Movahedpour, Hassan Ghasemi

引用次数: 0

Aspirin Challenge-Induced Genome-Wide DNA Methylation Profile of Peripheral Blood Lymphocytes in Aspirin-Exacerbated Respiratory Disease. 阿司匹林加重呼吸道疾病患者外周血淋巴细胞中阿司匹林挑战诱导的全基因组DNA甲基化谱图

DNA and cell biology Pub Date : 2024-03-01 Epub Date: 2024-02-22 DOI: 10.1089/dna.2023.0218

Jong-Uk Lee, Hun Soo Chang, Ji-Su Shim, Min-Hye Kim, Young-Joo Cho, Min Kyung Kim, Seung-Lee Park, Sun Ju Lee, Jong-Sook Park, Choon-Sik Park

{"title":"Aspirin Challenge-Induced Genome-Wide DNA Methylation Profile of Peripheral Blood Lymphocytes in Aspirin-Exacerbated Respiratory Disease.","authors":"Jong-Uk Lee, Hun Soo Chang, Ji-Su Shim, Min-Hye Kim, Young-Joo Cho, Min Kyung Kim, Seung-Lee Park, Sun Ju Lee, Jong-Sook Park, Choon-Sik Park","doi":"10.1089/dna.2023.0218","DOIUrl":"10.1089/dna.2023.0218","url":null,"abstract":"Genetic variation and epigenetic factors are thought to contribute to the development of hypersensitivity to aspirin. DNA methylation fluctuates dynamically throughout the day. To discover new CpG methylation in lymphocytes associated with aspirin-exacerbated respiratory disease (AERD), we evaluated changes in global CpG methylation profiles from before to after an oral aspirin challenge in patients with AERD and aspirin-tolerant asthma (ATA). Whole-genome CpG methylation levels of peripheral blood mononuclear cells were quantified with an Illumina 860K Infinium Methylation EPIC BeadChip array and then adjusted for inferred lymphocyte fraction (ILF) with GLINT and Tensor Composition Analysis. Among the 866,091 CpGs in the array, differentially methylated CpGs (DMCs) were found in 6 CpGs in samples from all 12 patients with asthma included in the study (AERD, n = 6; ATA, n = 6). DMCs were found in 3 CpGs in the 6 ATA samples and in 615 CpGs in the 6 AERD samples. A total of 663 DMCs in 415 genes and 214 intergenic regions differed significantly in the AERD compared with the ATA. In promoters, 126 CpG loci were predicted to bind to 38 transcription factors (TFs), many of which were factors already known to be involved in the pathogenesis of asthma and immune responses. In conclusion, we identified 615 new CpGs methylated in peripheral blood lymphocytes by oral aspirin challenge in AERD but not in ATA. These findings indicate that oral aspirin challenge induces epigenetic changes in ILFs, specifically in AERD patients, possibly via changes in TF binding, which may have epigenetic effects on the development of AERD.","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"132-140"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139934711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloning and Expression Analysis of Key Enzyme Gene CoGPPS Involved in Iridoid Glycoside Synthesis in Cornus officinalis. 参与山茱萸铱苷合成的关键酶基因 CoGPPS 的克隆和表达分析

DNA and cell biology Pub Date : 2024-03-01 Epub Date: 2024-02-13 DOI: 10.1089/dna.2023.0335

Jiaxi Chen, Xinjie Tan, Guangyang Guo, Panpan Wang, Hongxiao Zhang, Shufang Lv, Huawei Xu, Dianyun Hou

{"title":"Cloning and Expression Analysis of Key Enzyme Gene CoGPPS Involved in Iridoid Glycoside Synthesis in Cornus officinalis.","authors":"Jiaxi Chen, Xinjie Tan, Guangyang Guo, Panpan Wang, Hongxiao Zhang, Shufang Lv, Huawei Xu, Dianyun Hou","doi":"10.1089/dna.2023.0335","DOIUrl":"10.1089/dna.2023.0335","url":null,"abstract":"Cornus iridoid glycosides (CIGs), including loganin and morroniside, are the main active components of Cornus officinalis. As one of the key enzymes in the biosynthesis of CIGs, geranyl pyrophosphate synthase (GPPS) catalyzes the formation of geranyl pyrophosphate, which is the direct precursor of CIGs. In this study, the C. officinalis geranyl pyrophosphate synthase (CoGPPS) sequence was cloned from C. officinalis and analyzed. The cDNA sequence of the CoGPPS gene was 915 bp (GenBank No. OR725699). Phylogenetic analysis showed that CoGPPS was closely related to the GPPS sequence of Actinidia chinensis and Camellia sinensis, but relatively distantly related to Paeonia lactiflora and Tripterygium wilfordii. Results from the quantitative real-time PCR showed the spatiotemporal expression pattern of CoGPPS; that is, CoGPPS was specifically expressed in the fruits. Subcellular localization assay proved that CoGPPS was specifically found in chloroplasts. Loganin and morroniside contents in the tissues were detected by high-performance liquid chromatography, and both compounds were found to be at higher levels in the fruits than in leaves. Thus, this study laid the foundation for further studies on the synthetic pathway of CIGs.","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"125-131"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139731278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparing for the Next Pandemic: Increased Expression of Interferon-Stimulated Genes After Local Administration of Nasalferon or HeberNasvac. 为下一次大流行做准备：局部注射 Nasalferon 或 HeberNasvac 后干扰素刺激基因的表达增加。

DNA and cell biology Pub Date : 2024-02-01 Epub Date: 2023-12-21 DOI: 10.1089/dna.2023.0283

Jorge Agustín Aguiar Santiago, Maria Acelia Marrero Miragaya, Dariel Adrian Figueroa Oliva, Andres Aguilar Juanes, Arletis Idavoy Corona, Seknia Martínez Fernández, Ivis Morán Bertot, Meilyn Rodríguez Hernández, Eduardo Canales López, Ingrid Hernández Esteves, José Angel Silva Girado, Regla Caridad Estrada Vázquez, Omar Gell Cuesta, Yssel Mendoza-Marí, Iris Valdés Prado, Chabeli Rodríguez Ibarra, Daniel Octavio Palenzuela Gardon, Eduardo Pentón Arias, Gerardo Guillén Nieto, Julio Cesar Aguilar Rubido

{"title":"Preparing for the Next Pandemic: Increased Expression of Interferon-Stimulated Genes After Local Administration of Nasalferon or HeberNasvac.","authors":"Jorge Agustín Aguiar Santiago, Maria Acelia Marrero Miragaya, Dariel Adrian Figueroa Oliva, Andres Aguilar Juanes, Arletis Idavoy Corona, Seknia Martínez Fernández, Ivis Morán Bertot, Meilyn Rodríguez Hernández, Eduardo Canales López, Ingrid Hernández Esteves, José Angel Silva Girado, Regla Caridad Estrada Vázquez, Omar Gell Cuesta, Yssel Mendoza-Marí, Iris Valdés Prado, Chabeli Rodríguez Ibarra, Daniel Octavio Palenzuela Gardon, Eduardo Pentón Arias, Gerardo Guillén Nieto, Julio Cesar Aguilar Rubido","doi":"10.1089/dna.2023.0283","DOIUrl":"10.1089/dna.2023.0283","url":null,"abstract":"HeberNasvac, a therapeutic vaccine for chronic hepatitis B, is able to safely stimulate multiple Toll-like receptors, increasing antigen presentation in vitro and in a phase II clinical trial (Profira) in elderly volunteers who were household contacts of respiratory infection patients. Thus, a new indication as a postexposure prophylaxis or early therapy for respiratory infections has been proposed. In this study, we evaluated the expression of several interferon-stimulated genes (ISGs) after mucosal administration of HeberNasvac and compared this effect with the nasal delivery of interferon alpha 2b (Nasalferon). Molecular studies of blood samples of 50 subjects from the Profira clinical trial who were locally treated with HeberNasvac or Nasalferon and concurrent untreated individuals were compared based on their relative mRNA expression of OAS1, ISG15, ISG20, STAT1, STAT3, and DRB1-HLA II genes. In most cases, the gene expression induced by HeberNasvac was similar in profile and intensity to the expression induced by Nasalferon and significantly superior to that observed in untreated controls. The immune stimulatory effect of HeberNasvac on ISGs paved the way for its future use as an innate immunity stimulator in elderly persons and immunocompromised subjects or as part of Mambisa, a nasal vaccine to prevent severe acute respiratory syndrome coronavirus 2 infection.","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"95-102"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138833680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of Cellular-Signaling Pathways by Mammalian Proteins Containing Bacterial EPIYA or EPIYA-Like Motifs Predicted to be Phosphorylated. 含细菌 EPIYA 或 EPIYA 类似基团的哺乳动物蛋白质对细胞信号通路的调控。

DNA and cell biology Pub Date : 2024-02-01 Epub Date: 2023-12-28 DOI: 10.1089/dna.2023.0350

Mohammad Rasouli, Fatemeh Safari, Navid Sobhani, Mana Alavi, Raheleh Roudi

{"title":"Regulation of Cellular-Signaling Pathways by Mammalian Proteins Containing Bacterial EPIYA or EPIYA-Like Motifs Predicted to be Phosphorylated.","authors":"Mohammad Rasouli, Fatemeh Safari, Navid Sobhani, Mana Alavi, Raheleh Roudi","doi":"10.1089/dna.2023.0350","DOIUrl":"10.1089/dna.2023.0350","url":null,"abstract":"The effector proteins of several pathogenic bacteria contain the Glu-Pro-Ile-Tyr-Ala (EPIYA) motif or other similar motifs. The EPIYA motif is delivered into the host cells by type III and IV secretion systems, through which its tyrosine residue undergoes phosphorylation by host kinases. These motifs atypically interact with a wide range of Src homology 2 (SH2) domain-containing mammalian proteins through tyrosine phosphorylation, which leads to the perturbation of multiple signaling cascades, the spread of infection, and improved bacterial colonization. Interestingly, it has been reported that EPIYA (or EPIYA-like) motifs exist in mammalian proteomes and regulate mammalian cellular-signaling pathways, leading to homeostasis and disease pathophysiology. It is possible that pathogenic bacteria have exploited EPIYA (or EPIYA-like) motifs from mammalian proteins and that the mammalian EPIYA (or EPIYA-like) motifs have evolved to have highly specific interactions with SH2 domain-containing proteins. In this review, we focus on the regulation of mammalian cellular-signaling pathways by mammalian proteins containing these motifs.","PeriodicalId":93981,"journal":{"name":"DNA and cell biology","volume":" ","pages":"74-84"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139049996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acknowledgment of Reviewers 2023. 鸣谢 2023 年审稿人。

DNA and cell biology Pub Date : 2024-02-01 Epub Date: 2023-12-14 DOI: 10.1089/dna.2023.29025.ack

引用次数: 0