Clinical hemorheology and microcirculation最新文献_第2页

Diagnostic value of contrast-enhanced ultrasound in serum biomarker negative hepatocellular carcinoma and colorectal cancer liver metastasis. 超声造影对血清生物标志物阴性的肝细胞癌和结直肠癌肝转移的诊断价值。

Clinical hemorheology and microcirculation Pub Date : 2025-08-01 Epub Date: 2025-08-14 DOI: 10.1177/13860291251358021

Feihang Wang, Xiaolong Zhang, Yadan Xu, Qingyue Xu, Kai Yuan, Yi Dong, Wenping Wang

{"title":"Diagnostic value of contrast-enhanced ultrasound in serum biomarker negative hepatocellular carcinoma and colorectal cancer liver metastasis.","authors":"Feihang Wang, Xiaolong Zhang, Yadan Xu, Qingyue Xu, Kai Yuan, Yi Dong, Wenping Wang","doi":"10.1177/13860291251358021","DOIUrl":"10.1177/13860291251358021","url":null,"abstract":"PurposeTo explore the ultrasound features and the diagnostic values of CEUS LI-RADS and World Federation for Ultrasound in Medicine (WFUMB) - European Federation of Societies for Ultrasound in Medicine and Biology (EFSUMB) criteria in serum biomarker negative hepatocellular carcinoma (HCC) and colorectal cancer liver metastasis (CRLM).MethodsA total of 161 patients with pathologically diagnosed alpha-fetoprotein (AFP)-negative HCC and carcinoembryonic antigen (CEA)-negative CRLM were retrospectively enrolled, including 101 HCC patients and 60 CRLM patients. The clinical characteristics and ultrasound characteristics of all cases were analyzed and lesions were categorized via two criteria.ResultsThe rate of HBV infection in HCC patients was higher than CRLM patients (P < 0.001). For the liver parenchyma, the rate of liver cirrhosis and liver steatosis in HCC patients were both higher than CRLM patients (P < 0.001 and P = 0.048, respectively). On CEUS, HCC lesions depicted higher rate of arterial phase hyperenhancement (APHE), mild washout and later wash-out onset, while rim-hyperenhancement, marked washout and earlier wash-out onset were more frequently observed in CRLM lesions. For diagnosing HCC, the sensitivity, specificity and accuracy of CEUS LR-5 were 55.4%, 93.3% and 69.6%, respectively, and of WFUMB-EFSUMB criteria were 87.1%, 73.3% and 82.0%, respectively. For diagnosing CRLM, the sensitivity, specificity and accuracy of CEUS LR-M were 100.0%, 63.4% and 77.0%, respectively, and of WFUMB-EFSUMB criteria were 76.7%, 94.1% and 87.6%, respectively.ConclusionIn patients with serum biomarker negative HCC and CRLM, both CEUS LI-RADS and WFUMB-EFSUMB criteria have each advantages and have high application value.","PeriodicalId":93943,"journal":{"name":"Clinical hemorheology and microcirculation","volume":" ","pages":"158-164"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144850035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The value of ultrasound in the evaluation of the type 2 diabetes mellitus related plantar fascia changes. 超声对2型糖尿病相关足底筋膜改变的评价价值。

Clinical hemorheology and microcirculation Pub Date : 2025-07-01 Epub Date: 2025-07-07 DOI: 10.1177/13860291251356171

Shengqiao Wang, Xiuyun Lu, Juan Chen, Xinliang Xu, Jun Jiang, Yi Dong

{"title":"The value of ultrasound in the evaluation of the type 2 diabetes mellitus related plantar fascia changes.","authors":"Shengqiao Wang, Xiuyun Lu, Juan Chen, Xinliang Xu, Jun Jiang, Yi Dong","doi":"10.1177/13860291251356171","DOIUrl":"10.1177/13860291251356171","url":null,"abstract":"BackgroundPlantar fasciopathy is a common complication in diabetes patients.ObjectiveTo evaluate the effectiveness of ultrasound in detecting diabetic tendinopathy in type 2 diabetes mellitus (T2DM) patients by assessing plantar fascia (PF) thickness and stiffness.MethodsThis study included 70 T2DM patients with heel pain, diagnosed by American Diabetes Association (ADA). B mode ultrasound was used to evaluate PF thickness. Shear wave elastography (SWE) was used to assess PF stiffness.ResultsIn T2DM patients, the PF thickness (PFT) was significantly thicker (4.40 ± 0.79 mm vs. 2.87 ± 0.26 mm, P < 0.05) and the shear wave velocity (SWV) of PF was significantly lower (3.09 ± 0.57 m/s vs. 4.00 ± 1.01 m/s, P < 0.05) than the control group. PFT and SWV values were strongly correlated with age (r = 0.880, -0.888) and BMI (r = 0.814, -0.853). ROC analysis showed that PFT > 4.33 mm had a sensitivity of 98.0% and specificity of 77.2% for diabetic tendinopathy, with an area under the ROC curve (AUROC) of 0.956, SWV < 3.22 m/s had a sensitivity of 86.3% and specificity of 60.5% (AUROC = 0.789).ConclusionUltrasound is a non-invasive and efficient diagnostic tool for PF in T2DM patients.","PeriodicalId":93943,"journal":{"name":"Clinical hemorheology and microcirculation","volume":" ","pages":"122-130"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144577246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of cardiopulmonary bypass on hemorheology: Evaluation of clinical outcomes. 体外循环对血液流变学的影响：临床结果的评价。

Clinical hemorheology and microcirculation Pub Date : 2025-07-01 Epub Date: 2025-07-10 DOI: 10.1177/13860291251352316

Süleyman Aycan, Nazlı Helvacı, Alev Kural, Gözde Tekin, Mehmet Kızılay

{"title":"The effect of cardiopulmonary bypass on hemorheology: Evaluation of clinical outcomes.","authors":"Süleyman Aycan, Nazlı Helvacı, Alev Kural, Gözde Tekin, Mehmet Kızılay","doi":"10.1177/13860291251352316","DOIUrl":"10.1177/13860291251352316","url":null,"abstract":"BackgroundDuring cardiopulmonary bypass (CPB), which is frequently utilized in open-heart surgery, the maintenance of circulation in a non-physiological environment may lead to alterations in the properties of circulating blood components. In our study, we aimed to evaluate these changes using contemporary methodologies.MethodsThe study included 48 patients who underwent cardiac surgery with CPB. CPB duration and hypothermia degree were recorded and analyzed. Erythrocyte deformability (ED), erythrocyte aggregation (EA), whole blood viscosity (WBV), total oxidant status (TOS), and total antioxidant status (TAS) were measured; ED and EA were measured using LORRCA (RR Mechatronics, Hoorn, Netherlands).ResultsCompared to the preoperative period, a decrease in postoperative ED, EA, and WBV was observed. No significant change was detected in TAS levels; however, TOS levels were found to be elevated. No statistically significant correlation was observed between perioperative patient temperature, CPB duration, and ED, EA, or WBV values.ConclusionsIn patients undergoing surgery with CPB, a postoperative decrease in ED, EA, and WBV was demonstrated. Previous studies have suggested that the decline in ED is associated with blood transfusion. However, the significant reduction in ED observed in our study, even in patients who did not receive blood transfusions, is noteworthy.","PeriodicalId":93943,"journal":{"name":"Clinical hemorheology and microcirculation","volume":" ","pages":"105-115"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144602466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The m6A methyltransferase METTL14 governs NLRP3 and facilitates myocardial infarction via pyroptosis. m6A甲基转移酶METTL14控制NLRP3并通过焦亡促进心肌梗死。

Clinical hemorheology and microcirculation Pub Date : 2025-07-01 Epub Date: 2025-07-08 DOI: 10.1177/13860291251352301

Xiaoling Li, Longwei Zhou, Chengyan Yang, Keqin Wang, Yangjie Li, Sha Han, Xuan Gong, Ke Qin, Zengxue Lu

{"title":"The m6A methyltransferase METTL14 governs NLRP3 and facilitates myocardial infarction via pyroptosis.","authors":"Xiaoling Li, Longwei Zhou, Chengyan Yang, Keqin Wang, Yangjie Li, Sha Han, Xuan Gong, Ke Qin, Zengxue Lu","doi":"10.1177/13860291251352301","DOIUrl":"10.1177/13860291251352301","url":null,"abstract":"BackgroundN6-methyladenosine (m6A) methylation plays an important role in myocardial infarction, which is related to cardiomyocyte pyroptosis. This study aimed to explore the effects of the m6A methyltransferase, METTL14, on pyroptosis and the underlying mechanisms.Materials and methodsThe role of METTL14 in vivo was assessed by TTC staining, biochemical indicators, and H&E assays. Cell pyroptosis was evaluated by PI staining, IF, and western blot assay. The m6A methylation of NLRP3 was evaluated by RIP, dual-luciferase reporter assay, Me-RIP, and stability test.ResultsThe results indicated that METTL14 was highly expressed in LAD-ligated mice and OGD-induced cardiomyocytes. Depletion of METTL14 inhibited OGD-induced pyroptosis of cardiomyocytes and suppressed myocardial injury. Mechanically, METTL14 promoted m6A methylation of NLRP3 to enhance mRNA stability. Overexpression of NLRP3 reversed the effects of METTL14 knockdown on pyroptosis.ConclusionsMETTL14 knockdown attenuated cardiomyocyte pyroptosis and myocardial infarction by suppressing NLRP3 m6A methylation. The findings suggested that METTL14 has the potential to be the therapeutic target of myocardial infarction.","PeriodicalId":93943,"journal":{"name":"Clinical hemorheology and microcirculation","volume":" ","pages":"95-104"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144585786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using dynamic nomogram to modify TI-RADS and reduce the unnecessary FNA of thyroid nodules. 应用动态图修正TI-RADS，减少甲状腺结节的不必要FNA。

Clinical hemorheology and microcirculation Pub Date : 2025-07-01 Epub Date: 2025-07-17 DOI: 10.1177/13860291251357792

Jiahui Ni, Yunyun Liu, Xiaolong Li, Beibei Ye, Hui Shi, Ying Zhang, Yifeng Zhang

{"title":"Using dynamic nomogram to modify TI-RADS and reduce the unnecessary FNA of thyroid nodules.","authors":"Jiahui Ni, Yunyun Liu, Xiaolong Li, Beibei Ye, Hui Shi, Ying Zhang, Yifeng Zhang","doi":"10.1177/13860291251357792","DOIUrl":"10.1177/13860291251357792","url":null,"abstract":"Background: Current guidelines recommend Fine Needle Aspiration (FNA) based on nodule size and ultrasound characteristics; however, these guidelines still lead to a certain amount of unnecessary FNAs.Objective: To develop a dynamic nomogram prediction model based on the ACR Thyroid Imaging, Reporting, and Data System (TI-RADS) to reduce unnecessary FNAs.Methods: This multicenter study analyzed 3313 thyroid nodules undergoing FNA. Univariate and multivariate logistic regression models were constructed. Patients were divided into a training cohort and two validation cohorts to compare diagnostic performance and unnecessary FNAs.Results: This nomogram achieved performance of Area Under the Curve (AUC) 0.914 (95%CI: 0.894-0.934), 0.923 (95%CI: 0.900-0.946), 0.948 (95%CI: 0.918-0.978) in the training, internal and external validation cohort. Using this model, the unnecessary FNA rates for nodules in ACR TI-RADS category 3 (TR3) have decreased from 99.4% to 0%, in TR4 from 77.6% to 47.1%, and in TR5 from 25.4% to 18.9% in Center1, in TR3 have decreased from 91.9% to 0%, in TR4 from 60.0% to 21.1%, and in TR5 from 11.5% to 4.5% in Center 2 (p < 0.01).Conclusions: This dynamic nomogram achieved better prediction of malignant thyroid nodules compared with the mentioned risk stratification system, leading to a more rational FNA strategy.","PeriodicalId":93943,"journal":{"name":"Clinical hemorheology and microcirculation","volume":" ","pages":"131-144"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144661266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differences in oxygen-gradient ektacytometry parameters and blood viscosity between patients with sickle cell anemia and patients with sickle cell-hemoglobin C disorder. 镰状细胞性贫血与镰状细胞-血红蛋白C紊乱患者血粘度及氧梯度测定参数的差异

Clinical hemorheology and microcirculation Pub Date : 2025-07-01 Epub Date: 2025-07-08 DOI: 10.1177/13860291251357796

Philippe Connes, Marie Martin, Camille Boisson, Emeric Stauffer, Céline Renoux, Philippe Joly, Solene Poutrel, Carine Halfon-Domenech, Alexandra Gauthier, Elie Nader

{"title":"Differences in oxygen-gradient ektacytometry parameters and blood viscosity between patients with sickle cell anemia and patients with sickle cell-hemoglobin C disorder.","authors":"Philippe Connes, Marie Martin, Camille Boisson, Emeric Stauffer, Céline Renoux, Philippe Joly, Solene Poutrel, Carine Halfon-Domenech, Alexandra Gauthier, Elie Nader","doi":"10.1177/13860291251357796","DOIUrl":"10.1177/13860291251357796","url":null,"abstract":"Oxygen-gradient ektacytometry (oxygenscan) has been recently used in the context of sickle cell disease (SCD) to determine the ability of red blood cells (RBCs) to deform in normoxia and during deoxygenation. While several studies focused on the determinants of oxygenscan parameters in the homozygous form of SCD (i.e., HbSS), very few studies focused on hemoglobin SC disease (HbSC). Oxygenscan parameters were compared between HbSS (N = 304) and HbSC (N = 58) patients, with or without hydroxyurea (HU). RBC deformability measured in normoxia or at low oxygen level was lower in HbSS patients without HU compared to HbSS patients with HU and HbSC patients with or without HU. The propensity of RBCs to sickle during deoxygenation was greater in HbSS than in HbSC patients. Although no significant impact of HU was observed on RBC deformability/sickling in HbSC patients, those with HU had lower blood viscosity. The ability of RBC to recover their maximum deformability after a deoxygenation/reoxygenation cycle was lower in HbSC compared to HbSS patients. Finally, oxygenscan parameters and routine hematological parameters correlated differently in HbSS and HbSC patients. Our study showed difference in oxygenscan parameters between HbSS and HbSC individuals that could partly explain the difference in the clinical expression of SCD.","PeriodicalId":93943,"journal":{"name":"Clinical hemorheology and microcirculation","volume":" ","pages":"145-151"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144585784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental investigation of proangiogenic properties of sacubitril on a chorio allantoic membrane model. 苏比利在绒毛膜尿囊膜模型上促血管生成的实验研究。

Clinical hemorheology and microcirculation Pub Date : 2025-07-01 Epub Date: 2025-07-08 DOI: 10.1177/13860291251352350

Mustafa Ozgul, Eyup Aydogan

{"title":"Experimental investigation of proangiogenic properties of sacubitril on a chorio allantoic membrane model.","authors":"Mustafa Ozgul, Eyup Aydogan","doi":"10.1177/13860291251352350","DOIUrl":"10.1177/13860291251352350","url":null,"abstract":"BackgroundSacubitril is an important and effective agent for cardiovascular remodeling. This study aims to evaluate its proangiogenic effects in an experimental model.MethodsThe chorioallantoic membrane (CAM) model was created for the investigation of the proangiogenic potential of sacubitril with different therapeutic doses (10-7 M, 10-6 M, 10-5 M) and compared with drug-free pellet group (sham) and normal morphology (control) of chick embryo development in drug-free chick embryos. The developing embryo's vascularity and the pellets' effect were evaluated under a stereoscopic microscope. The density of vascular shoots and newly formed vascular nodules were not recorded.ResultsThere was no significant difference between the control and drug-free pellet groups (12.4 ± 2.8 vs. 14.1 ± 1.3 junctions per ROI, p = 0.48). The incremental angiogenic properties were detected in drug groups as follows: 15.3 ± 3.8 per ROI in Group I (10-7 M concentration); 21.6 ± 5.4 per ROI in Group II (10-6 M concentration); 22.9 ± 8.1 per ROI in Group III (10-5 M concentration) (p < 0.001).ConclusionOur findings support that sacubitril provokes angiogenesis in a dose-dependent manner. Investigating these properties can be useful for understanding further effects of this agent in other cardiovascular diseases. Therapeutic angiogenesis is important for ameliorating the results.","PeriodicalId":93943,"journal":{"name":"Clinical hemorheology and microcirculation","volume":" ","pages":"116-121"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144585785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In situ assessment of renal cortical microcirculation in septic acute kidney injury rats using contrast-enhanced ultrasound and sidestream dark-field imaging. 对比增强超声和侧流暗场成像原位评价脓毒性急性肾损伤大鼠肾皮质微循环。

Clinical hemorheology and microcirculation Pub Date : 2025-06-01 Epub Date: 2025-06-10 DOI: 10.1177/13860291251347870

Wei Su, Tian Li, Jin Lin, Xinjie Guo, Ran Pang, Meili Duan, Jingfeng Liu

{"title":"In situ assessment of renal cortical microcirculation in septic acute kidney injury rats using contrast-enhanced ultrasound and sidestream dark-field imaging.","authors":"Wei Su, Tian Li, Jin Lin, Xinjie Guo, Ran Pang, Meili Duan, Jingfeng Liu","doi":"10.1177/13860291251347870","DOIUrl":"10.1177/13860291251347870","url":null,"abstract":"Purpose: To evaluate in situ assessment of renal cortical microcirculation (RCM) in S-AKI rats using CEUS and SDF imaging.Methods: CLP was performed to induce S-AKI in Sprague-Dawley rats; Sham rats served as control. CEUS and SDF imaging were sequentially applied at postoperative 24 h and 48 h to investigate the RCM.Results: At postoperative 24 h and 48 h, CLP rats exhibited hemodynamic deterioration and AKI. Renal injury score and positive staining for pimonidazole were significantly increased (p < 0.05). In CLP rats, parameters of RCM, including peak enhancement (PE), rise time (RT), PE/RT, time to peak (TTP), total small vessel density (TVD), perfused small vessel density (PVD), microvascular flow index (MFI), and proportion of perfused vessels (PPV) were significantly deteriorated compared to Sham rats (p < 0.05). In CLP rats, Lac was significantly negatively correlated with PE, PE/RT, TVD, PVD, PPV and MFI, and significantly positively correlated with RT and TTP. TVD, PVD, PPV and MFI were significantly positively correlated with PE and PE/RT and negatively correlated with RT and TTP.Conclusion: The RCM of S-AKI rats exhibited decreased blood perfusion and heterogeneity in early sepsis. CEUS and SDF imaging are effective methods for detecting the RCM.","PeriodicalId":93943,"journal":{"name":"Clinical hemorheology and microcirculation","volume":" ","pages":"60-72"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144259567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-379-3p activates the MAPK/JNK/p38 pathway by negatively targeting DUSP1 expression to promote myocardial ischemia-reperfusion injury. miR-379-3p通过负向靶向DUSP1表达激活MAPK/JNK/p38通路，促进心肌缺血-再灌注损伤。

Clinical hemorheology and microcirculation Pub Date : 2025-06-01 Epub Date: 2025-06-22 DOI: 10.1177/13860291251348806

Wei Yang, Xiangfeng Bai, Yao Tang, Ziting Ren, Wendong Chen

{"title":"miR-379-3p activates the MAPK/JNK/p38 pathway by negatively targeting DUSP1 expression to promote myocardial ischemia-reperfusion injury.","authors":"Wei Yang, Xiangfeng Bai, Yao Tang, Ziting Ren, Wendong Chen","doi":"10.1177/13860291251348806","DOIUrl":"10.1177/13860291251348806","url":null,"abstract":"BackgroundMyocardial ischemia-reperfusion injury (MI/RI) is a primary cause of cardiomyocyte death in various cardiovascular diseases. MicroRNAs (miRNAs) play significant roles in MI/RI.ObjectiveThis study aims to elucidate the function and mechanism of miR-379-3p in this context.MethodsThe Cell Counting Kit 8 (CCK-8) and flow cytometry were utilized to assess the proliferation and apoptosis of cardiomyocytes. The expression levels of cardiomyocyte injury-related proteins, including LDHA, cTnT, CK-MB, and cTnI, were measured using enzyme-linked immunoassay (ELISA) kits. The targeted regulatory relationship between miR-379-3p and dual-specificity protein phosphatase 1 (DUSP1) was investigated through dual luciferase reporter gene assays. Additionally, the expression levels of miR-379-3p/DUSP1 and mitogen-activated protein kinases (MAPKs) signaling pathway in MI/RI were evaluated using Western blotting.ResultsThe expression of miR-379-3p was significantly elevated in MI/RI, and the inhibition of miR-379-3p notably alleviated MI/RI. The specific binding of miR-379-3p to the 3'UTR of DUSP1 mRNA was confirmed by dual luciferase reporter gene assays. Furthermore, miR-379-3p exacerbated cardiomyocyte injury by negatively regulating DUSP1. Ultimately, miR-379-3p activated the MAPK/JNK/p38 pathway by downregulating DUSP1 expression, thereby promoting MI/RI.ConclusionsmiR-379-3p activates the MAPK/JNK/p38 pathway through the negative regulation of DUSP1 expression to promote MI/RI.","PeriodicalId":93943,"journal":{"name":"Clinical hemorheology and microcirculation","volume":" ","pages":"73-83"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of high-frequency ultrasound in occult focal liver lesions: Does it help? 高频超声在隐匿性局灶性肝病变中的应用：有帮助吗？

Clinical hemorheology and microcirculation Pub Date : 2025-06-01 Epub Date: 2025-06-25 DOI: 10.1177/13860291251348805

Hong Qin, Zheng-Biao Ji, Yu-Li Zhu, Qian-Nan Zhao, Kai Yuan, Dan Zuo, Xiao-Long Zhang, Yi Dong, Wen-Ping Wang

{"title":"Application of high-frequency ultrasound in occult focal liver lesions: Does it help?","authors":"Hong Qin, Zheng-Biao Ji, Yu-Li Zhu, Qian-Nan Zhao, Kai Yuan, Dan Zuo, Xiao-Long Zhang, Yi Dong, Wen-Ping Wang","doi":"10.1177/13860291251348805","DOIUrl":"10.1177/13860291251348805","url":null,"abstract":"ObjectivesTo investigate the role of high-frequency ultrasound in detection of occult focal liver lesions (FLLs).MethodsIn this retrospective study from April 2016 to March 2019, eighty-five patients with indistinctive FLLs were included. High-frequency and low-frequency B-mode ultrasound (BMUS) and contrast-enhanced ultrasound (CEUS) were performed for each lesion. The detection rates of two methods and the ultrasound performance of co-detected lesions were compared. Subgroup analysis was performed according to different liver backgrounds.ResultsFor 85 FLLs with mean diameter of 11.80 ± 4.66 mm and mean depth of 20.91 ± 6.89 mm, high-frequency BMUS detected 77 (90.6%) lesions, significantly more than low-frequency BMUS (36, 42.4%, p < 0.001). Among the lesions co-detected by low-frequency and high-frequency ultrasound, the lesions at high-frequency mainly showed heterogeneous echogenicity (p < 0.001), regular morphology (p < 0.001), well-defined boundary (p < 0.001) and inhomogeneous enhancement (p < 0.001) in arterial phase. Even in the presence of cirrhosis or fatty liver, high-frequency ultrasound still performed excellently.ConclusionHigh-frequency ultrasound improves the detection of small and superficially located FLLs.","PeriodicalId":93943,"journal":{"name":"Clinical hemorheology and microcirculation","volume":" ","pages":"84-92"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0