Clinical hemorheology and microcirculation最新文献

{"title":"Lipoprotein subfractions, erythrocyte membrane cholesterol in coronary artery disease: A cross-sectional clinical study.","authors":"Hüseyin Avni Uydu, Mehmet Bostan","doi":"10.1177/13860291261490206","DOIUrl":"https://doi.org/10.1177/13860291261490206","url":null,"abstract":"<p><p>BackgroundAtherosclerosis is a lipid-driven inflammatory disease in which plasma lipoproteins and cellular cholesterol pools contribute to coronary artery disease (CAD). Lipoprotein subfractions and erythrocyte membrane cholesterol (EMC) have each been linked to CAD, but their combined behavior across angiographic stages remains insufficiently characterized. EMC may also be relevant to blood flow properties and microcirculatory function.MethodsWe studied 122 patients undergoing coronary angiography and 64 controls. CAD severity was classified by the number of diseased vessels. Plasma lipid profile, LDL and HDL subfractions, atherogenic indices, EMC, and C-reactive protein were analyzed using multivariable regression, ANCOVA, ROC analysis, and an exploratory multi-marker approach.ResultsConventional lipid parameters showed limited group differences, whereas sdLDL, HDL subfractions, EMC, CRP, and selected lipid ratios differed across CAD severity groups. EMC increased across CAD categories and remained associated with CAD after adjustment for conventional lipid variables. Among lipid-related variables, EMC showed the most consistent association with angiographically defined CAD.ConclusionsEMC may provide complementary hemorheology- and microcirculation-relevant information in angiographically defined CAD. Lipoprotein subfractions and composite lipid indices may offer adjunctive characterization of CAD-related lipid remodeling.</p>","PeriodicalId":93943,"journal":{"name":"Clinical hemorheology and microcirculation","volume":" ","pages":"13860291261490206"},"PeriodicalIF":0.0,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149186899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic and functional changes in apheresis platelets during cold and room temperature storage: A pilot in vitro study.","authors":"Aidos Kenzhin, Saniya Abdrakhmanova, Serik Baidurin, Zharas Bibekov, Mariya Akhayeva, Yevgeniy Merenkov","doi":"10.1177/13860291261489729","DOIUrl":"https://doi.org/10.1177/13860291261489729","url":null,"abstract":"<p><strong>Background: </strong>Platelet concentrates are conventionally stored at room temperature (20-24°C), which preserves platelet viability but is associated with progressive metabolic deterioration and increased risk of bacterial contamination. Cold storage (4°C) has re-emerged as a potential alternative strategy; however, its impact on platelet metabolism, activation, and hemostatic function during extended storage remains incompletely characterized.</p><p><strong>Aim: </strong>To evaluate metabolic and functional changes in apheresis platelet concentrates during storage under cold and room temperature conditions.</p><p><strong>Methods: </strong>Apheresis platelet concentrates obtained from two healthy donors were stored in platelet additive solution with or without pathogen inactivation. Platelets were stored at 20-24°C (up to 7 days) or 4°C (up to 21 days). Metabolic parameters (pH, glucose, lactate), activation markers (CD62P, Annexin V), and hemostatic function (thromboelastography) were assessed at predefined time points. Because this was an exploratory pilot study with two independent donors, the analysis was primarily descriptive and no inferential statistical comparisons were performed.</p><p><strong>Results: </strong>Cold storage was associated with slower glucose consumption and lower lactate accumulation during the early storage period, accompanied by a progressive decline in pH during prolonged storage. Cold-stored platelets showed increased CD62P expression and phosphatidylserine exposure, as reflected by Annexin V binding, particularly during prolonged storage. Thromboelastographic analysis showed relative preservation of clot strength during the first 7 days of cold storage, with mean maximum amplitude values remaining approximately 72-79 mm during this period.</p><p><strong>Conclusion: </strong>Cold storage was associated with distinct metabolic and functional changes, including slower early glucose consumption, lower lactate accumulation, increased platelet activation and phosphatidylserine exposure, and relative preservation of clot strength during the first 7 days. Given the exploratory design and limited sample size, these findings should be considered preliminary and require confirmation in larger experimental and clinical studies.</p>","PeriodicalId":93943,"journal":{"name":"Clinical hemorheology and microcirculation","volume":" ","pages":"13860291261489729"},"PeriodicalIF":0.0,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149186882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calculated whole blood viscosity and erythrocytes parameters in plasma cell dyscrasias: Insights from MGUS and SMM.","authors":"Melania Carlisi, Rosalia Lo Presti, Salvatrice Mancuso, Anna Di Giorgio, Beatrice Salamone, Miriam Sostegno, Sergio Siragusa, Gregorio Caimi","doi":"10.1177/13860291261493017","DOIUrl":"https://doi.org/10.1177/13860291261493017","url":null,"abstract":"<p><strong>Background and objective: </strong>In the plasma cell dyscrasias, monoclonal gammopathy of undetermined significance and smoldering multiple myeloma represent the asymptomatic form, but, in their clinical course we can observe some pathological conditions. For this reason, our aim is to evaluate calculated whole blood viscosity and some erythrocyte indices, to obtain information on mechanisms underlying thrombotic risk and microcirculatory alterations.</p><p><strong>Patients and methods: </strong>We retrospectively evaluated calculated whole blood viscosity (according to the de Simone formula), hematocrit, plasma protein concentration, RDW, hemoglobin, RDW/albumin and RDW/hemoglobin ratios in 50 subjects with MGUS [median age 64.00 (16.059)] and in 93 SMM patients [median age 69.00 (13.00)].</p><p><strong>Results: </strong>From the data comparison, obtained with the Mann-Whitney test, it emerges that the hematocrit value, is lower in patients with SMM unlike the plasma protein values which are higher in this nosographic entity. Serum albumin is significantly reduced in subjects with MGUS, while RDW and the RDW/albumin ratio are higher in these same subjects. No statistically significant results were obtained by subdividing each nosographic entity according to both isotype (IgA and IgG) and risk level.</p><p><strong>Conclusions: </strong>The evaluated erythrocyte indices could represent a simple and accessible parameters for a better clinical evaluation, and further risk stratification, warranting a next prospective validation.</p>","PeriodicalId":93943,"journal":{"name":"Clinical hemorheology and microcirculation","volume":" ","pages":"13860291261493017"},"PeriodicalIF":0.0,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149083167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intraoperative monitoring of malignant liver tumor microwave ablation using contrast-enhanced ultrasound: A preliminary study.","authors":"Ivor Dropco, Nina Käser, Ulrich Kaiser, Paul Kupke, Hans Jürgen Schlitt, Stefan M Brunner, Christina Hackl, Christian Stroszczynski, Ernst Michael Jung","doi":"10.1177/13860291261491824","DOIUrl":"https://doi.org/10.1177/13860291261491824","url":null,"abstract":"<p><p>ObjectiveThis study aimed to evaluate intraoperative assessment and monitoring of liver tumor ablations using contrast-enhanced ultrasound (CEUS).MethodsTwenty patients (11 males [65%] and 9 females [45%]) with malignant lesions (cholangiocellular carcinoma (CCC), 1; hepatocellular carcinoma (HCC), 5; colorectal liver metastases (CRLM), 13; and one patient with alveolar echinococcosis) were examined intraoperatively with CEUS. Images were acquired in cine loops, covering the early arterial phase (0-15 s) up to the portal venous phase (1 min). Ablation was performed under real-time ultrasound guidance using microwave ablation (MWA). The generator output was adjusted up to 80 watts, up to 10 min. Post-ablation CEUS was conducted for immediate control.ResultsTwenty patients underwent ablation procedures. Ablation was carried out under ultrasound guidance with adjusted output settings between 40 and 80 watts and durations ranging from 2 to 5 min. Post-interventional CEUS confirmed complete devascularization in all planes. All patients underwent track ablation using 70 watts. At 6-month follow-up, no local recurrence was observed.ConclusionThe preliminary findings demonstrate the feasibility of intraoperative CEUS during microwave ablation of liver lesions. The technique proves to be a safe and effective intraoperative tool, owing to its ability to provide verification of complete tumor devascularization.</p>","PeriodicalId":93943,"journal":{"name":"Clinical hemorheology and microcirculation","volume":" ","pages":"13860291261491824"},"PeriodicalIF":0.0,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149077065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clot microstructure in infective and non-infective exacerbation of COPD, are they different?","authors":"Suresh Pillai, Matthew Lawrence, Jun-Cezar Zaldua, Karl Hawkins, Keith Morris, Janet Whitley, Phillip A Evans","doi":"10.1177/13860291261489728","DOIUrl":"https://doi.org/10.1177/13860291261489728","url":null,"abstract":"<p><p>ObjectivePatients with Chronic Obstructive Pulmonary Disease (COPD) often develop exacerbations that could be infective or non-infective and are prone to develop thrombosis. To assess thrombogenicity in this group, a functional biomarker of clot microstructure namely fractal dimension (<i>d</i>_f) is utilised. This sub-group analysis intent to compare the changes in <i>d</i>_f between infective and non-infective exacerbation of COPD.Methods85 patients with acute exacerbations of COPD were recruited from the Emergency Department. Blood samples were taken to determine biomarkers of inflammation, haemostasis and rheology at four time points (0 h, 4-6 h, 24 h and 3-7 days).ResultsThe biomarker of clot microstructure did not attain significance in the infective group when compared to the non-infective group at four time points.ConclusionsThe study demonstrates that exacerbation caused by infection does not cause more thrombogenicity compared to non-infective causes utilising clot microstructure.</p>","PeriodicalId":93943,"journal":{"name":"Clinical hemorheology and microcirculation","volume":" ","pages":"13860291261489728"},"PeriodicalIF":0.0,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148993091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A.L. Copley Best Paper Prize 2024.","authors":"Christian Lehmann, Philippe Connes","doi":"10.1177/13860291261484283","DOIUrl":"https://doi.org/10.1177/13860291261484283","url":null,"abstract":"","PeriodicalId":93943,"journal":{"name":"Clinical hemorheology and microcirculation","volume":" ","pages":"13860291261484283"},"PeriodicalIF":0.0,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148993156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Red blood cell aggregation and atherosclerosis: Curvilinear clinical implications.","authors":"Yingying Wang, Yiwen Chen, Cong Yan","doi":"10.1177/13860291261470519","DOIUrl":"10.1177/13860291261470519","url":null,"abstract":"<p><p>The progression of atherosclerosis is closely associated with red blood cell (RBC) aggregation. By increasing blood viscosity and reducing shear stress, RBC aggregation induces blood flow stasis and microcirculatory dysfunction, thereby facilitating lipid deposition within the vascular wall. Moreover, aggregated RBCs activate endothelial inflammatory responses and trigger oxidative stress, forming a pathological cycle characterized by \"aggregation-inflammation-oxidation.\" The red blood cell aggregation index (EAI) correlates with the severity of atherosclerosis and may serve as a potential biomarker for early diagnosis. In integrative Chinese and Western medical approaches, blood-activating and stasis-resolving traditional Chinese medicines exhibit multitarget regulatory effects, particularly in improving hemorheological properties and suppressing inflammation, providing novel insights into the prevention and treatment of atherosclerosis.</p>","PeriodicalId":93943,"journal":{"name":"Clinical hemorheology and microcirculation","volume":" ","pages":"60-75"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149027235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental characterization of blood rheology and development of a computational rheological Index during progressive hemorrhagic shock.","authors":"Maka Mantskava, Nana Momtselidze, Giorgi Kuchava, Lena Davlianidze","doi":"10.1177/13860291261484056","DOIUrl":"10.1177/13860291261484056","url":null,"abstract":"<p><p><b>Background:</b> Hemorrhagic shock is associated with profound disturbances of microcirculatory perfusion in which alterations of blood rheology may contribute to tissue hypoxia and organ dysfunction. Historically, the concept of hemorrhagic shock has evolved from an initial focus on systemic hemodynamic failure toward recognition of the critical role of microcirculatory dysfunction and impaired tissue oxygen delivery. More recent experimental and clinical research has further emphasized the contribution of blood rheological abnormalities to the progression of hemorrhagic shock and organ dysfunction. However, the relative contribution of individual hemorheological parameters during progressive hemorrhagic shock remains insufficiently characterized. <b>Objective:</b> To investigate stage-dependent changes in blood rheology in an experimental rat model of hemorrhagic shock and to evaluate the relationship between conventional hemorheological parameters and a newly proposed computational rheological index derived from routine hematological variables. <b>Methods:</b> Hemorrhagic shock was induced in 60 anesthetized rats by controlled withdrawal of 2.0, 2.5, or 3.5 mL of arterial blood, corresponding to progressive stages of shock. 20 intact animals served as controls. Erythrocyte aggregation, erythrocyte deformability, plasma viscosity, hematocrit, and the computational rheological index were assessed 15 min after blood withdrawal. <b>Results:</b> Progressive blood loss resulted in stage-dependent deterioration of hemorheological properties. Erythrocyte aggregation demonstrated the greatest relative increase and was strongly associated with shock severity. Changes in erythrocyte deformability were less pronounced, whereas plasma viscosity and hematocrit remained relatively stable throughout the experimental stages. The computational rheological index exhibited changes parallel to erythrocyte aggregation, suggesting that it reflects progressive rheological impairment. <b>Conclusions:</b> Experimental hemorrhagic shock is accompanied by progressive deterioration of blood rheology, with erythrocyte aggregation representing the most sensitive laboratory marker. The proposed computational rheological index demonstrated a close association with experimentally observed rheological alterations and warrants further validation in independent experimental and clinical studies.</p>","PeriodicalId":93943,"journal":{"name":"Clinical hemorheology and microcirculation","volume":" ","pages":"81-90"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148972385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dalcetrapib decreases red blood cell sickling propensity in sickle cell disease.","authors":"Claire Bordat, Elie Nader, Philippe Connes, Philippe Joly, Solene Poutrel, Anne Perez, Eric Niesor","doi":"10.1177/13860291261475024","DOIUrl":"10.1177/13860291261475024","url":null,"abstract":"<p><p>The aim of the present study was to compare the effects of dalcetrapib and voxelotor on the rheology of red blood cells (RBCs) from sickle cell patients, as well as on hemoglobin oxygen affinity. Oxygen gradient ektacytometry was used to determine RBC deformability in normoxia and hypoxia, as well as the propensity of RBCs to sickle. Hemoglobin oxygen affinity was also measured following dalcetrapib and voxelotor incubation. Both voxelotor and dalcetrapib increased RBC deformability in hypoxia and decreased the propensity of RBCs to sickle under deoxygenation. However, in contrast to voxelotor, the affinity of hemoglobin S (HbS) to oxygen was not affected by dalcetrapib. The combination of both voxelotor and dalcetrapib led to greater RBC rheological improvement. Our findings suggest that dalcetrapib could inhibit RBC sickling during deoxygenation.</p>","PeriodicalId":93943,"journal":{"name":"Clinical hemorheology and microcirculation","volume":" ","pages":"76-80"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148726561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new chapter: Charting the future of <i>Clinical Hemorheology and Microcirculation</i>.","authors":"Christian Lehmann, Philippe Connes","doi":"10.1177/13860291261488124","DOIUrl":"10.1177/13860291261488124","url":null,"abstract":"","PeriodicalId":93943,"journal":{"name":"Clinical hemorheology and microcirculation","volume":" ","pages":"59"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148946365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}