Bioscience of microbiota, food and health最新文献

{"title":"Effect of a fructooligosaccharides fraction from <i>Psacalium decompositum</i> on the intestinal bacterial microbiota of rats.","authors":"María Guadalupe Juárez-Pérez, Francisco J Alarcón-Aguilar, J Iván Jasso-Villagómez, M Margarita Canales-Martínez, Jorge E Campos-Contreras, Héctor Escalona-Buendía, Francisco J Fernández","doi":"10.12938/bmfh.2025-032","DOIUrl":"10.12938/bmfh.2025-032","url":null,"abstract":"<p><p><i>Psacalium decompositum</i> is a shrub indigenous to northern Mexico and Arizona and New Mexico in the USA. It has been utilized in traditional medicine for the treatment of many ailments, including rheumatic disorders, pain, and diabetes. A chemical analysis of a hexane extract from the rhizomes of <i>P. decompositum</i> revealed the presence of sesquiterpenes, including cacalol, cacalone, maturin, and maturinone, among others. Furthermore, its aqueous fraction was subjected to isolation, resulting in the identification of linear fructooligosaccharides with an eight-unit polymerisation degree (LFOS8). The objective of this study was to ascertain the impact of LFOS8 derived from <i>P. decompositum</i> on the intestinal microbiota of rats through 16S rRNA gene sequencing and bioinformatics analysis. Male Wistar rats were randomly assigned to the following groups (n=8): a control group administered an isotonic saline solution, positive control group treated with chicory inulin (a prebiotic), and experimental group treated with LFOS8 fraction. The positive control and experimental groups of animals demonstrated alterations in their gut microbiota, resulting in an increase in the growth of beneficial bacteria, including bacteria that are considered probiotics and produce butyrate. This research provides insights into the potential therapeutic benefits of <i>P. decompositum</i>.</p>","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":"45 1","pages":"1-8"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12765533/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145907229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Source-dependent antimicrobial activity of plant-derived lactic acid bacteria against periodontal pathogens.","authors":"Yuji Tsujikawa, Junya Yamamoto, Mami Naito, Iwao Sakane","doi":"10.12938/bmfh.2025-055","DOIUrl":"10.12938/bmfh.2025-055","url":null,"abstract":"<p><p>Periodontal disease is a prevalent inflammatory condition linked to tooth loss and systemic disorders. Plant-derived lactic acid bacteria (LAB) have emerged as promising probiotics for oral health. In this study, 80 LAB strains were isolated from tea leaves, young barley leaves, carrots, and fermented vegetables, and their antimicrobial effects against key periodontal pathogens were evaluated. Various assays, including biofilm inhibition, growth inhibition, disk diffusion, and RT-qPCR, were employed to assess activity. LAB strains from tea leaves and young barley leaves exhibited significantly stronger anti-biofilm activity and growth inhibition against <i>Porphyromonas gingivalis</i> than those from carrots and fermented vegetables. These strains also demonstrated notable antimicrobial activity against <i>P. gingivalis</i> and <i>Fusobacterium nucleatum</i> and significantly downregulated key virulence-related genes, including <i>fimA</i>, <i>kgp</i>, and <i>rgpA</i> in <i>P. gingivalis</i>. The results suggest that the antimicrobial efficacy of LAB is dependent on their plant source. Tea and barley-derived LAB strains may serve as potent candidates for probiotic development aimed at preventing periodontal disease. Further investigation is warranted.</p>","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":"45 2","pages":"149-157"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13083155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147724964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Premature stop codons in <i>inlA</i> reduce Caco-2 cell invasion by <i>Listeria monocytogenes</i> and contribute to serotype bias in human clinical isolates.","authors":"Abdullah Jubair, Hiroyuki Chiba, I Hsun Huang, Akinobu Kajikawa, Masataka Uchino, Shizunobu Igimi","doi":"10.12938/bmfh.2026-028","DOIUrl":"https://doi.org/10.12938/bmfh.2026-028","url":null,"abstract":"<p><p><i>Listeria monocytogenes</i> is a highly lethal foodborne pathogen that exhibits strain-dependent differences in virulence. Infection begins with traversal of the intestinal barrier through a two-step process involving adhesion to intestinal epithelial cells followed by intracellular invasion. In this study, Caco-2 cells were used to investigate these early infection events in 25 Japanese <i>L. monocytogenes</i> isolates (9 human clinical, 14 food-derived, and 2 environmental isolates) and 1 reference clinical strain (ScottA). We examined the genetic integrity of key adhesion- and invasion-related virulence genes (<i>lap, fbpA, inlA,</i> and <i>inlB</i>) and their relationships with adhesion and invasion phenotypes. Premature stop codons (PMSCs) were identified in <i>inlA</i> in 11 isolates and in <i>inlB</i> in 15 isolates. PMSCs in <i>inlA</i> were absent from all clinical isolates and predominantly found in serotypes 1/2a and 1/2c, which are rarely associated with clinical listeriosis. Also, three novel <i>inlA</i> PMSC variants were identified. Adhesion to Caco-2 cells was relatively uniform among isolates (5.87-6.88 log₁₀ colony-forming units [CFU]/mL), whereas invasion varied markedly (2.74-6.27 log₁₀ CFU/mL). Isolates carrying PMSCs in <i>inlA</i> consistently showed reduced invasion (<4 log₁₀ CFU/mL), whereas isolates lacking PMSCs exhibited significantly higher invasion (>5 log₁₀ CFU/mL). Western blot analysis confirmed the absence of full-length InlA in PMSC-containing isolates. Antibody-mediated inhibition of InlA produced a dose-dependent reduction in invasion, confirming the critical role of InlA in epithelial cell invasion. These findings demonstrate that the PMSCs in <i>inlA</i> are a key determinant of invasion capacity and may partly explain the serotype bias observed among human clinical isolates. Our results highlight the importance of virulence factor-based risk assessment for <i>L. monocytogenes</i>, rather than treating all strains as equally pathogenic.</p>","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":"45 4","pages":"284-292"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13659320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149186340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An altered gut microbiota is associated with anthropometric and biochemical changes in a prediabetes Mexican cohort.","authors":"Fernando Suárez-Sánchez, Luis Fernando Alvarado-Vera, Valentina Tremaroli, Graciela Castro-Escarpulli, Jesús Peralta-Romero, Miguel Cruz-López","doi":"10.12938/bmfh.2025-036","DOIUrl":"10.12938/bmfh.2025-036","url":null,"abstract":"<p><p>An altered gut microbiota is considered to be an environmental factor contributing to the pathogenesis of various diseases, including metabolic disorders. In this study, we identified changes in the composition of the fecal microbiota of Mexican individuals with prediabetes and analyzed their influences on variables related to this metabolic disorder. Bacterial DNA was extracted from feces of normoglycemic and prediabetes individuals, followed by sequencing of the V4 16S rDNA. Clinical variables were correlated with bacterial abundance and diversity indexes. LEfSe analysis was used to identify relevant bacteria in normoglycemic and prediabetes individuals. Relationships between bacterial clusters and clinical data were assessed with weighted correlation network analysis (WGCNA). Bacterial diversity was positively correlated with anthropometric variables in the control group, while in individuals with prediabetes, it was negatively correlated with the insulin concentration and homeostatic model assessment of insulin resistance (HOMA-IR). We also observed that the microbiota in prediabetes individuals had a higher prevalence of <i>Ralstonia</i> and was enriched with bacteria belonging to the family <i>Lachnospiraceae</i>, as well as <i>Eubacterium biforme</i> and <i>Gemmiger formicilis</i>, while in controls, we found higher abundances of <i>Coprococcus</i> and <i>Bifidobacterium bifidum</i> (abs(linear discriminant analysis (LDA) score) >2). These results identify changes in the microbiota in Mexican adults with prediabetes. Remarkably, the genus <i>Ralstonia</i> was only found in prediabetes individuals. Higher insulin concentrations and HOMA-IR in those individuals were correlated with lower microbiota diversity.</p>","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":"45 1","pages":"19-26"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12765534/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145907261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of supplementing a <i>Saccharomyces cerevisiae</i>-fermentation-derived postbiotic in sow diet on pathogenic and antibiotic-resistant bacteria in a production system with high biosecurity.","authors":"Le Dinh Phung, Pham Hoang Son Hung, Le Duc Thao, Ho Thi Dung, Nguyen Thi Hoa, Bui Thi Hien, Anjan Mondal, Victor Nsereko, Nguyen VAN Chao","doi":"10.12938/bmfh.2025-038","DOIUrl":"10.12938/bmfh.2025-038","url":null,"abstract":"<p><p>This study evaluated the effects of <i>Saccharomyces cerevisiae</i>-fermentation-derived postbiotic (SCFP) on pathogenic and antimicrobial-resistant (AMR) bacteria in sows within a high biosecurity system. Thirty sows were divided into three groups: a standard basal diet (CON group), CON with 1.0 kg/MT beta-glucan 50% (BG-50 group), and CON with 2.0 kg/MT SCFP (SCFP group). Fecal samples were collected at day 0, 60 days of gestation, and farrowing for enumeration of <i>Escherichia coli</i> and isolation of <i>Salmonella</i>. Isolates were assessed for AMR and antimicrobial resistance genes (ARGs) using disk diffusion and polymerase chain reaction (PCR) methods. The results showed that <i>E. coli</i> counts (log10 CFU/g) were significantly reduced in the SCFP group compared with the CON group (p=0.03). The SCFP decreased the frequency of <i>E. coli</i> isolates with resistance to chloramphenicol and tetracycline (p<0.05), while BG-50 reduced resistance to chloramphenicol, doxycycline, erythromycin, trimethoprim-sulfamethoxazole, and tetracycline (p<0.05). The mean inhibition zone for most antimicrobials against <i>E. coli</i> increased with gestation (p<0.05). Overall, dietary SCFP supplementation alleviated <i>E. coli</i> counts and the frequency of <i>Salmonella</i>. In addition, the effect of dietary SCFP and BG-50 supplementation on the level of AMR in <i>E. coli</i> and <i>Salmonella</i> was observed.</p>","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":"45 2","pages":"100-114"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13083193/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147725001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shenling Baizhu powder associated with alleviating inflammation and intestinal mucosal microbiota injury in mice with diarrhea induced by a lard diet and fatigued state.","authors":"Wenjing Huang, Jing Liu, Miao Jiang, Ying Cai, Zhoujin Tan","doi":"10.12938/bmfh.2026-043","DOIUrl":"https://doi.org/10.12938/bmfh.2026-043","url":null,"abstract":"<p><p>The intestinal microbiota plays a crucial role in the onset of diarrhea and can be modulated through dietary changes. Shenling Baizhu powder (SLBZ) has been shown to regulate diarrhea. Thirty Kunming mice were randomly divided into a normal group (MCN), model group (MMD), and SLBZ group (MSLBZ). Diarrhea was induced by fatigue combined with a high-fat diet. After modeling, the MSLBZ group received 145.6 g/(kg·d) SLBZ for 7 days. Pathological examination of small intestine tissues, along with measurements of interleukin 6 (IL-6), interleukin 17 (IL-17), immunoglobulin A (sIgA), and mucin 2 (Muc2) levels, were conducted. The intestinal mucosa was extracted for high-throughput sequencing of 16S rRNA. In the MMD group, intestinal damage, lymphocyte infiltration, decreased sIgA, diarrhea, and a significant increase in <i>Helicobacter</i> were observed compared with the MCN group. After SLBZ treatment, the MSLBZ group exhibited improved body weight, reduced intestinal damage, decreased levels of IL-6, increased sIgA, and alleviated diarrhea. Compared with the MMD group, the abundance of <i>Duncaniella freteri</i> was significantly higher, while <i>Helicobacter</i> was markedly reduced. Correlation analysis showed that <i>Duncaniella freteri</i> was positively correlated with sIgA and Muc2 and negatively correlated with IL-6 and IL-17. SLBZ alleviates intestinal mucosal damage, reduces pro-inflammatory cytokines, enhances sIgA production, and restores the microbiota balance in mice with diarrhea.</p>","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":"45 4","pages":"307-318"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13659319/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149186392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of Sishen Pill: modulating intestinal mucosal microbiota and barrier function in diarrhea-predominant irritable bowel syndrome with Spleen-Kidney Yang Deficiency.","authors":"Liwen Li, Qi Long, Zhoujin Tan, Na Deng","doi":"10.12938/bmfh.2026-016","DOIUrl":"https://doi.org/10.12938/bmfh.2026-016","url":null,"abstract":"<p><p>This study aimed to investigate the intestinal microecological mechanisms underlying the effects of Sishen Pill (SSP) on diarrhea-predominant irritable bowel syndrome (IBS-D) with Spleen-Kidney Yang Deficiency Syndrome, thereby providing experimental evidence for clinical management. Murine models of IBS-D characterized by Spleen-Kidney Yang deficiency were established in SPF-grade female Kunming mice by <i>Folium senna</i>-adenine gavage in conjunction with restraint-tail clamping. Experimental groups received SSP decoction or pinaverium bromide suspension. Intestinal morphological changes were assessed via Alcian blue staining. The concentrations of serum D-lactate (D-LA) and diamine oxidase (DAO) were measured through enzyme-linked immunosorbent assay. The intestinal mucosal microbiota was analyzed using 16S rRNA gene sequencing. The model mice exhibited reduced anal temperatures, diarrhea, weight loss, intestinal mucosal barrier injury, and altered mucosal microbiota. SSP treatment alleviated these abnormalities, decreased serum D-LA and DAO levels, and improved the intestinal mucosal structure. Microbiota analysis identified <i>Alkaliphilus</i> as a potential genus associated with SSP intervention, while <i>Streptococcus</i> and <i>Maribacter</i> were correlated with intestinal barrier indicators. These findings suggest that SSP may improve IBS-D with Spleen-Kidney Yang Deficiency by modulating the intestinal mucosal microbiota and restoring mucosal barrier function.</p>","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":"45 4","pages":"237-253"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13658991/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149186450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between <i>Lactococcus cremoris</i> subsp. <i>cremoris</i> FC intake and microbiota profiles in relation to bowel movement: a post hoc analysis of a placebo-controlled, parallel-group trial.","authors":"Yoshiyuki Tabata, Yayoi Gotoh, Masamichi Watanabe, Kentaro Maruyama","doi":"10.12938/bmfh.2026-001","DOIUrl":"10.12938/bmfh.2026-001","url":null,"abstract":"<p><p><i>Lactococcus cremoris</i> subsp. <i>cremoris</i> FC (<i>L. cremoris</i> FC) improves bowel movement; however, its associations with the intestinal microbiota remain unclear. To address this gap, this post hoc analysis of a four-week, placebo-controlled, parallel-group trial evaluates the associations between <i>L. cremoris</i> FC intake and gut microbiota profiles. We analyzed fecal samples from 83 healthy Japanese adults with mild constipation who received either placebo capsules containing corn starch (n=20) or <i>L. cremoris</i> FC capsules (50, 75, or 100 mg; n=21 each). Using 16S rRNA gene amplicon sequencing, we evaluated diversity and relative abundance and explored association patterns through Sparse InversE Covariance estimation for Ecological Association and Statistical Inference network analysis. Although no dose dependence was observed, alpha diversity decreased significantly in the <i>L. cremoris</i> FC 100 mg group, whereas beta diversity shifted significantly in the 50 mg group. Short-chain fatty acid producing genera, including <i>Blautia</i>, <i>Anaerobutyricum</i>, <i>Anaerostipes</i>, and <i>Agathobaculum</i>, showed higher relative abundances after intervention in both <i>L. cremoris</i> FC intake groups. Moreover, the relative abundance of <i>Blautia</i> was positively correlated with stool characteristics across post-intervention samples. Exploratory network analysis indicated altered statistical associations among bacterial taxa. <i>Blautia</i> and <i>Roseburia</i> showed higher centrality in the <i>L. cremoris</i> FC 100 mg group, whereas <i>Bacteroides</i> and <i>Ruminococcus</i> were more central in the placebo group. These findings suggest that <i>L. cremoris</i> FC intake was associated with coordinated alterations in microbial diversity, genus-level relative abundance, and association patterns, providing ecological insights into microbiota changes accompanying bowel improvement, though underlying mechanisms remain unclear.</p>","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":"45 3","pages":"217-224"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13322762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148371257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiota: a potential therapeutic target for hyperuricemia and gout.","authors":"Jiahui Tang, Yaxi Liu, Nianwei Wu, Jie Lu, Yuwei Zhang, Nanwei Tong, Qingguo Lü","doi":"10.12938/bmfh.2025-042","DOIUrl":"10.12938/bmfh.2025-042","url":null,"abstract":"<p><p>The prevalence of hyperuricemia (HUA) and gout has increased in recent decades. Current therapeutic approaches for HUA/gout are often limited by potential risks, necessitating the exploration of safer and more effective treatment options. Emerging evidence highlights the gut microbiota as a pivotal regulator of uric acid (UA) homeostasis. This review synthesizes current advances in microbiota-targeted interventions for HUA/gout, focusing on mechanistic insights and translational potential. We aim to provide a roadmap for optimizing microbiota-based therapies in HUA/gout management by bridging mechanistic discoveries with clinical translation. Gut microbiota can mitigate HUA/gout through several mechanisms, including regulating UA and purine metabolism, alleviating inflammation and modulating immune response, and enhancing the integrity of the intestinal barrier. Therapeutic strategies targeting gut microbiota include probiotics, prebiotics, traditional Chinese medicine, and fecal microbiota transplantation, which offer multi-target and multi-pathway benefits. While these microbiota-targeted therapies offer advantages over conventional drugs, several challenges remain. Future research should prioritize mechanistic elucidation, personalized microbiota modulation, and large-scale trials to optimize therapeutic paradigms for HUA/gout.</p>","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":"45 2","pages":"85-99"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13083157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147724979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiota alters the Th1/Th2 balance and aggravates food-allergic enteropathy in a mouse model.","authors":"Kosuke Nishitsuji, Tomohiro Hoshino, Masato Tsuda, Yoshiyo Bamba, Shigeru Kakuta, Hiroyuki Adachi, Naoki Shimojo, Huijuan Jia, Akira Hosono, Kazuhiro Hirayama, Satoshi Hachimura, Haruyo Nakajima-Adachi","doi":"10.12938/bmfh.2025-106","DOIUrl":"https://doi.org/10.12938/bmfh.2025-106","url":null,"abstract":"<p><p>In non-IgE-mediated food allergy, food protein-induced enteropathy (FPE) can be evoked by the Th2 response. Some reports show that either Th2-biased immune responses or gut microbial dysbiosis can exacerbate food-allergic gastrointestinal inflammation. Nevertheless, it remains unclear whether gut microbiota directly affect Th2-biased immune responses involving FPE. To address this issue, we used OVA23-3 × BALB/cA mice, which is an FPE-like model. Feeding the mice an egg-white diet (EW) induced excessive Th2-type immune responses in CD4⁺ T cells in the mesenteric lymph nodes (MLNs), resulting in weight loss and enteropathy. Firstly, we compared specific pathogen-free (SPF) OVA23-3 × BALB/cA mice with germ-free (GF) OVA23-3 × BALB/cA mice when fed an EW diet. Symptoms shown in SPF mice were attenuated in GF mice, suggesting that gut microbiota may contribute to exacerbating symptoms. Additionally, we orally administered broad-spectrum antibiotics (Abx) to OVA23-3×BALB/cA mice and analyzed the gut microbiota. Compared with a no-Abx administration group, we found that Abx administration significantly altered the alpha and beta diversity of the gut microbiota in EW-fed mice. The presence of <i>Alloprevotella</i>, <i>Bacteroides</i>, and <i>Muribaculaceae</i> was reduced to approximately 10%. Both weight loss and enteropathy in EW-fed mice were ameliorated after Abx administration. Furthermore, administration of Abx to the EW-fed group improved the Th1/Th2 balance in MLN cells and decreased the mRNA expression of IL-33 and the aryl hydrocarbon receptor in the jejunum. These results suggest that some members of the gut microbiota disturb Th1/Th2 responses in the MLN, by affecting the small intestinal epithelium, thereby exacerbating Th2-biased responses and inducing food-allergic enteropathy.</p>","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":"45 4","pages":"293-306"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13659322/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}