Biomedical and environmental sciences : BES最新文献_第4页

HIV Pretreatment Drug Resistance and Transmission Clusters among Newly Diagnosed Patients in the China-Myanmar Border Region, 2020-2023. 2020-2023年中缅边境地区新发感染者HIV预处理耐药及传播聚集性分析

IF 4.1

Biomedical and environmental sciences : BES Pub Date : 2025-07-20 DOI: 10.3967/bes2025.080

Huan Liu, Yue Cheng Yang, Xing Duan, Yi Chen Jin, Yan Fen Cao, Yi Feng, Chang Cai, He He Zhao, Hou Lin Tang

{"title":"HIV Pretreatment Drug Resistance and Transmission Clusters among Newly Diagnosed Patients in the China-Myanmar Border Region, 2020-2023.","authors":"Huan Liu, Yue Cheng Yang, Xing Duan, Yi Chen Jin, Yan Fen Cao, Yi Feng, Chang Cai, He He Zhao, Hou Lin Tang","doi":"10.3967/bes2025.080","DOIUrl":"10.3967/bes2025.080","url":null,"abstract":"Objective: This study aimed to investigate the prevalence of HIV pretreatment drug resistance (PDR) and the transmission clusters associated with PDR-related mutations in newly diagnosed, treatment-naive patients between 2020 and 2023 in Dehong prefecture, Yunnan province, China.Methods: Demographic information and plasma samples were collected from study participants. PDR was assessed using the Stanford HIV Drug Resistance Database. The Tamura-Nei 93 model within HIV-TRACE was employed to compute pairwise matches with a genetic distance of 0.015 substitutions per site.Results: Among 948 treatment-naive individuals with eligible sequences, 36 HIV subtypes were identified, with unique recombinant forms (URFs) being the most prevalent (18.8%, 178/948). The overall prevalence of PDR was 12.4% (118/948), and resistance to non-nucleotide reverse transcriptase inhibitors (NNRTIs), nucleotide reverse transcriptase inhibitors (NRTIs), and protease inhibitors (PIs) was 10.7%, 1.3%, and 1.6%, respectively. A total of 91 clusters were identified, among which eight showed evidence of PDR strain transmission. The largest PDR-associated cluster consisted of six CRF01_AE drug-resistant strains carrying K103N and V179T mutations; five of these individuals had initial CD4+ cell counts < 200 cells/μL.Conclusion: The distribution of HIV subtypes in Dehong is diverse and complex. PDR was moderately prevalent (12.4%) between 2020 and 2023. Evidence of transmission of CRF01_AE strains carrying K103N and V179T mutations was found. Routine surveillance of PDR and the strengthening of control measures are essential to limit the spread of drug-resistance HIV strains.","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"38 7","pages":"840-847"},"PeriodicalIF":4.1,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144877259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemic Evolution Trends and Spatiotemporal Clustering of Human Brucellosis in Xilingol League Inner Mongolia, from 2004 to 2023. 2004 - 2023年内蒙古锡林郭勒盟人间布鲁氏菌病流行演变趋势及时空聚类分析

IF 4.1

Biomedical and environmental sciences : BES Pub Date : 2025-07-20 DOI: 10.3967/bes2025.081

Zhi Guo Liu, Miao Wang, Hao Tang, Chui Zhao Xue, Zhen Jun Li, Can Jun Zheng

{"title":"Epidemic Evolution Trends and Spatiotemporal Clustering of Human Brucellosis in Xilingol League Inner Mongolia, from 2004 to 2023.","authors":"Zhi Guo Liu, Miao Wang, Hao Tang, Chui Zhao Xue, Zhen Jun Li, Can Jun Zheng","doi":"10.3967/bes2025.081","DOIUrl":"10.3967/bes2025.081","url":null,"abstract":"Objective: Human brucellosis is a serious public health concern in the Xilingol League, Inner Mongolia; however, the epidemic trends are unclear.Method: In this study, Joinpoint regression analysis and spatiotemporal analysis were applied to investigate the epidemic evolution of human brucellosis.Result: From 2004 to 2023, a total of 35,747 cases were reported, with an annual average of 1787.35 cases and an annual average incidence rate of 176.04/100,000. The incidence increased from 173.96/100,000 in 2004 to 500.71/100,000 in 2009 and fluctuated to 61.43/100,000 in 2023. Three epidemic join points were observed in which the disease experienced an alternative rise and fall, peaking in 2009 (APC = 21.73, P > 0.001) and 2020 (APC = 21.51, P > 0.001). The disease showed a persistent decline trend in lentitude (AAPC = -5.30, P > 0.001), suggesting challenges in disease control and a higher risk of rebound. The most cases were reported in Xilinhot City ( n = 4,777), followed by 4,391 in Sonid Left Banner, and 4,324 in Abaga Banner. Spatiotemporal analysis revealed two high clusters (CI and CII) from 2005 to 2012, the high cluster encompassing eight counties and shifting from north to south.Conclusion: The present analysis highlights that human brucellosis has decreased significantly in the Xilingol League, but the epidemic is still severe; further implementation of a strict control program is necessary.","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"38 7","pages":"848-855"},"PeriodicalIF":4.1,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144877258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Socioeconomic and Environmental Factors Influence Tuberculosis Control. 社会经济和环境因素影响结核病控制。

IF 4.1

Biomedical and environmental sciences : BES Pub Date : 2025-07-20 DOI: 10.3967/bes2025.091

Lei Gao

引用次数: 0

Trends Analysis on Vaccination Coverage of Influenza in Shandong Province, China. 山东省流感疫苗接种率趋势分析

IF 4.1

Biomedical and environmental sciences : BES Pub Date : 2025-07-20 DOI: 10.3967/bes2025.063

Gui Jie Luan, Liang Sun, Wei Yan Zhang, Yao Liu, Shao Nan Liu

引用次数: 0

Comparative Transcriptomic and Metabolomic Analyses Reveal the Mechanism by Which Foam Macrophages Restrict Survival of Intracellular Mycobacterium Tuberculosis. 比较转录组学和代谢组学分析揭示泡沫巨噬细胞限制胞内结核分枝杆菌存活的机制。

IF 4.1

Biomedical and environmental sciences : BES Pub Date : 2025-07-20 DOI: 10.3967/bes2025.078

Xiao Peng, Yuan Yuan Liu, Li Yao Chen, Hui Yang, Yan Chang, Ye Ran Yang, Xuan Zhang, An Na Jia, Yong Bo Yu, Yong Li Guo, Jie Lu

{"title":"Comparative Transcriptomic and Metabolomic Analyses Reveal the Mechanism by Which Foam Macrophages Restrict Survival of Intracellular Mycobacterium Tuberculosis.","authors":"Xiao Peng, Yuan Yuan Liu, Li Yao Chen, Hui Yang, Yan Chang, Ye Ran Yang, Xuan Zhang, An Na Jia, Yong Bo Yu, Yong Li Guo, Jie Lu","doi":"10.3967/bes2025.078","DOIUrl":"10.3967/bes2025.078","url":null,"abstract":"Objectives: This study aimed to investigate the impact of foam macrophages (FMs) on the intracellular survival of Mycobacterium tuberculosis (MTB) and identify the molecular mechanisms influencing MTB survival.Methods: An in vitro FM model was established using oleic acid induction. Transcriptomic and metabolomic analyses were conducted to identify the key molecular pathways involved in FM-mediated MTB survival.Results: Induced FMs effectively restricted MTB survival. Transcriptomic and metabolomic profiling revealed distinct changes in gene and metabolite expression in FMs during MTB infection compared with normal macrophages. Integrated analyses identified significant alterations in the cyclic adenosine monophosphate (cAMP) signaling pathway, indicating that its activation contributes to the FM-mediated restriction of MTB survival.Conclusions: FMs inhibit MTB survival. The cAMP signaling pathway is a key contributor. These findings enhance the understanding of the role of FMs in tuberculosis progression, suggest potential targets for host-directed therapies, and offer new directions for developing diagnostic and therapeutic strategies against tuberculosis.","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"38 7","pages":"781-791"},"PeriodicalIF":4.1,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144877255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering Virulence Factors of Hyper-Virulent Pseudomonas aeruginosa Associated with Meningitis. 破译与脑膜炎相关的高毒力铜绿假单胞菌的毒力因子。

IF 4.1

Biomedical and environmental sciences : BES Pub Date : 2025-07-20 DOI: 10.3967/bes2025.082

Li Ling Xie, Shuo Liu, Yu Fan Wang, Ming Chun Li, Zhen Hua Huang, Yue Ma, Qi Lin Yu

{"title":"Deciphering Virulence Factors of Hyper-Virulent Pseudomonas aeruginosa Associated with Meningitis.","authors":"Li Ling Xie, Shuo Liu, Yu Fan Wang, Ming Chun Li, Zhen Hua Huang, Yue Ma, Qi Lin Yu","doi":"10.3967/bes2025.082","DOIUrl":"10.3967/bes2025.082","url":null,"abstract":"Objective: Pseudomonas aeruginosa( P. aeruginosa) is a prevalent pathogenic bacterium involved in meningitis; however, the virulence factors contributing to this disease remain poorly understood.Methods: The virulence of the P. aeruginosa A584, isolated from meningitis samples, was evaluated by constructing in vitro blood-brain barrier and in vivo systemic infection models. qPCR, whole-genome sequencing, and drug efflux assays of A584 were performed to analyze the virulence factors.Results: Genomic sequencing showed that A584 formed a phylogenetic cluster with the reference strains NY7610, DDRC3, Pa58, and Pa124. Its genome includes abundant virulence factors, such as hemolysin, the Type IV secretion system, and pyoverdine. A584 is a multidrug-resistant strain, and its wide-spectrum resistance is associated with enhanced drug efflux. Moreover, this strain caused significantly more severe damage to the blood-brain barrier than the standard strain, PAO1. qPCR assays further revealed the downregulation of the blood-brain barrier-associated proteins Claudin-5 and Occludin by A584. During systemic infection, A584 exhibited a higher capacity of brain colonization than PAO1 (37.1 × 10 6 CFU/g brain versus 2.5 × 10 6 CFU/g brain), leading to higher levels of the pro-inflammatory factors IL-1β and TNF-α.Conclusion: This study sheds light on the virulence factors of P. aeruginosa involved in meningitis.","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"38 7","pages":"856-866"},"PeriodicalIF":4.1,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144877256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased Tertiary Lymphoid Structures are Associated with Exaggerated Lung Tissue Damage in Smokers with Pulmonary Tuberculosis. 吸烟者肺结核患者三级淋巴结构增加与肺组织损伤加重有关。

IF 4.1

Biomedical and environmental sciences : BES Pub Date : 2025-07-20 DOI: 10.3967/bes2025.020

Yue Zhang, Liang Li, Zi Kang Sheng, Ya Fei Rao, Xiang Zhu, Yu Pang, Meng Qiu Gao, Xiao Yan Gai, Yong Chang Sun

{"title":"Increased Tertiary Lymphoid Structures are Associated with Exaggerated Lung Tissue Damage in Smokers with Pulmonary Tuberculosis.","authors":"Yue Zhang, Liang Li, Zi Kang Sheng, Ya Fei Rao, Xiang Zhu, Yu Pang, Meng Qiu Gao, Xiao Yan Gai, Yong Chang Sun","doi":"10.3967/bes2025.020","DOIUrl":"10.3967/bes2025.020","url":null,"abstract":"Objective: Cigarette smoking exacerbates the progression of pulmonary tuberculosis (TB). The role of tertiary lymphoid structures (TLS) in chronic lung diseases has gained attention; however, it remains unclear whether smoking-exacerbated lung damage in TB is associated with TLS. This study aimed to analyze the characteristics of pulmonary TLS in smokers with TB and to explore the possible role of TLS in smoking-related lung injury in TB.Methods: Lung tissues from 36 male patients (18 smokers and 18 non-smokers) who underwent surgical resection for pulmonary TB were included in this study. Pathological and immunohistological analyses were conducted to evaluate the quantity of TLS, and chest computed tomography (CT) was used to assess the severity of lung lesions. The correlation between the TLS quantity and TB lesion severity scores was analyzed. The immune cells and chemokines involved in TLS formation were also evaluated and compared between smokers and non-smokers.Results: Smoker patients with TB had significantly higher TLS than non-smokers ( P < 0.001). The TLS quantity in both the lung parenchyma and peribronchial regions correlated with TB lesion severity on chest CT (parenchyma: r = 0.5767; peribronchial: r = 0.7373; both P < 0.001). Immunohistochemical analysis showed increased B cells, T cells, and C-X-C motif chemokine ligand 13 (CXCL13) expression in smoker patients with TB ( P < 0.001).Conclusion: Smoker TB patients exhibited increased pulmonary TLS, which was associated with exacerbated lung lesions on chest CT, suggesting that cigarette smoking may exacerbate lung damage by promoting TLS formation.","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"38 7","pages":"810-818"},"PeriodicalIF":4.1,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144877260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial-temporal Dynamics of Tuberculosis and Its Association with Meteorological Factors and Air Pollution in Shaanxi Province, China. 陕西省肺结核的时空变化及其与气象因子和大气污染的关系

IF 4.1

Biomedical and environmental sciences : BES Pub Date : 2025-07-20 DOI: 10.3967/bes2025.083

Heng Liang Lyu, Xi Hao Liu, Hui Chen, Xue Li Zhang, Feng Liu, Zi Tong Zheng, Hong Wei Zhang, Yuan Yong Xu, Wen Yi Zhang

引用次数: 0

Droplet Digital PCR for Diagnosing Brucellosis Spondylitis: Method Development and Evaluation. 微滴数字PCR诊断布鲁氏菌病脊柱炎：方法开发与评价。

IF 4.1

Biomedical and environmental sciences : BES Pub Date : 2025-07-20 DOI: 10.3967/bes2025.085

Xiu Qin Chang, Guang Tian Liu, Bo Li, Meng Hao, Xue Feng Jiang, Zhi Guo Liu

引用次数: 0

Spatio-Temporal Pattern and Socio-economic Influencing Factors of Tuberculosis Incidence in Guangdong Province: A Bayesian Spatiotemporal Analysis. 广东省结核病发病时空格局及社会经济影响因素：贝叶斯时空分析

IF 4.1

Biomedical and environmental sciences : BES Pub Date : 2025-07-20 DOI: 10.3967/bes2025.071

Hui Zhong Wu, Xing Li, Jia Wen Wang, Rong Hua Jian, Jian Xiong Hu, Yi Jun Hu, Yi Ting Xu, Jianpeng Xiao, Ai Qiong Jin, Liang Chen

{"title":"Spatio-Temporal Pattern and Socio-economic Influencing Factors of Tuberculosis Incidence in Guangdong Province: A Bayesian Spatiotemporal Analysis.","authors":"Hui Zhong Wu, Xing Li, Jia Wen Wang, Rong Hua Jian, Jian Xiong Hu, Yi Jun Hu, Yi Ting Xu, Jianpeng Xiao, Ai Qiong Jin, Liang Chen","doi":"10.3967/bes2025.071","DOIUrl":"10.3967/bes2025.071","url":null,"abstract":"Objective: To investigate the spatiotemporal patterns and socioeconomic factors influencing the incidence of tuberculosis (TB) in the Guangdong Province between 2010 and 2019.Method: Spatial and temporal variations in TB incidence were mapped using heat maps and hierarchical clustering. Socioenvironmental influencing factors were evaluated using a Bayesian spatiotemporal conditional autoregressive (ST-CAR) model.Results: Annual incidence of TB in Guangdong decreased from 91.85/100,000 in 2010 to 53.06/100,000 in 2019. Spatial hotspots were found in northeastern Guangdong, particularly in Heyuan, Shanwei, and Shantou, while Shenzhen, Dongguan, and Foshan had the lowest rates in the Pearl River Delta. The ST-CAR model showed that the TB risk was lower with higher per capita Gross Domestic Product (GDP) [Relative Risk ( RR), 0.91; 95% Confidence Interval ( CI): 0.86-0.98], more the ratio of licensed physicians and physician ( RR, 0.94; 95% CI: 0.90-0.98), and higher per capita public expenditure ( RR, 0.94; 95% CI: 0.90-0.97), with a marginal effect of population density ( RR, 0.86; 95% CI: 0.86-1.00).Conclusion: The incidence of TB in Guangdong varies spatially and temporally. Areas with poor economic conditions and insufficient healthcare resources are at an increased risk of TB infection. Strategies focusing on equitable health resource distribution and economic development are the key to TB control.","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"38 7","pages":"819-828"},"PeriodicalIF":4.1,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144877265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0