Biomedical and environmental sciences : BES最新文献

{"title":"Antibiotic-Depleted Lung Microbiota Modulates Surfactant Proteins Expression and Reduces Experimental Silicosis.","authors":"Qiang Zhou, Mei Yu Chang, Ning Li, Yi Guan, San Qiao Yao","doi":"10.3967/bes2025.012","DOIUrl":"https://doi.org/10.3967/bes2025.012","url":null,"abstract":"<p><strong>Objective: </strong>Recent studies have overturned the traditional concept of the lung as a \"sterile organ\" revealing that pulmonary microbiota dysbiosis and abnormal surfactant proteins (SPs) expression are involved in the progression of silicosis. This study aimed to investigate the relationship between abnormal SPs expression and dysbiosis of lung microbiota in silica-induced lung fibrosis, providing insights into mechanisms of silicosis.</p><p><strong>Methods: </strong>Lung pathology, SPs expression, and microbiota composition were evaluated in silica-exposed mice. A mouse model of antibiotic-induced microbiota depletion was established, and alveolar structure and SPs expression were assessed. The roles of the lung microbiota and SPs in silicosis progression were further evaluated in mice with antibiotic-induced microbiota depletion, both with and without silica exposure.</p><p><strong>Results: </strong>Silica exposure induced lung inflammation and fibrosis, along with increased expression of SP-A expression. Antibiotics (Abx)-induced microbiota depletion elevated SP-A and SP-D expression. Furthermore, silica exposure altered lung microbiota composition, enriching potentially pathogenic taxa. However, antibiotic-induced microbiota depletion prior to silica exposure reduced silica-mediated lung fibrosis and inflammation.</p><p><strong>Conclusion: </strong>Lung microbiota is associated with silica-induced lung injury. Overproduction of SP-A and SP-D, induced by Abx-induced microbiota depletion, may enhance the resistance of mouse lung tissue to silica-induced injury.</p>","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"38 4","pages":"469-483"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of High-Risk Populations: The Cornerstone of Effective and Cost-Efficient Cancer Screening.","authors":"Wei Cao, Fei Wang, Ni Li","doi":"10.3967/bes2025.044","DOIUrl":"https://doi.org/10.3967/bes2025.044","url":null,"abstract":"","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"38 4","pages":"401-402"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a Prognostic Model for Lysosome-dependent Cell Death in Gastric Cancer Based on Single-cell RNA-seq and Bulk RNA-seq Data.","authors":"Peng Ni, Kai Xin Guo, Tian Yi Liang, Xin Shuang Fan, Yan Qiao Hua, Yang Ye Gao, Shuai Yin Chen, Guang Cai Duan, Rong Guang Zhang","doi":"10.3967/bes2024.159","DOIUrl":"https://doi.org/10.3967/bes2024.159","url":null,"abstract":"<p><strong>Objective: </strong>To identify prognostic genes associated with lysosome-dependent cell death (LDCD) in patients with gastric cancer (GC).</p><p><strong>Methods: </strong>Differentially expressed genes (DEGs) were identified using The Cancer Genome Atlas - Stomach Adenocarcinoma. Weighted gene co-expression network analysis was performed to identify the key module genes associated with LDCD score. Candidate genes were identified by DEGs and key module genes. Univariate Cox regression analysis, and least absolute shrinkage and selection operator regression and multivariate Cox regression analyses were performed for the selection of prognostic genes, and risk module was established. Subsequently, key cells were identified in the single-cell dataset (GSE183904), and prognostic gene expression was analyzed. Cell proliferation and migration were assessed using the Cell Counting Kit-8 assay and the wound healing assay.</p><p><strong>Results: </strong>A total of 4,465 DEGs, 95 candidate genes, and 4 prognostic genes, including <i>C19orf59</i>, <i>BATF2</i>, <i>TNFAIP2</i>, and <i>TNFSF18</i>, were identified in the analysis. Receiver operating characteristic curves indicated the excellent predictive power of the risk model. Three key cell types (B cells, chief cells, and endothelial/pericyte cells) were identified in the GSE183904 dataset. <i>C19orf59</i> and <i>TNFAIP2</i> exhibited predominant expression in macrophage species, whereas <i>TNFAIP2</i> evolved over time in endothelial/pericyte cells and chief cells. Functional experiments confirmed that interfering with <i>C19orf59</i> inhibited proliferation and migration in GC cells.</p><p><strong>Conclusion: </strong><i>C19orf59</i>, <i>BATF2</i>, <i>TNFAIP2</i>, and <i>TNFSF18</i> are prognostic genes associated with LDCD in GC. Furthermore, the risk model established in this study showed robust predictive power.</p>","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"38 4","pages":"416-432"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144046246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk Factors and Predictive Model for Acute Mountain Sickness among Han Chinese Travelers to Xizang Autonomous Region.","authors":"Qian Hui Gong, Qiong Li, Zhi Chao Xu, Xiao Wei Chen, Xiao Bing Shen","doi":"10.3967/bes2025.030","DOIUrl":"https://doi.org/10.3967/bes2025.030","url":null,"abstract":"","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"38 4","pages":"506-510"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144063100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of the Gut-Brain Axis in Chronotype-Driven Alzheimer's Disease: A Mendelian Randomization Study.","authors":"Jing Ting Kong, Meng Xue Wang, Xue Zi Zhang, Zan Wang, Qing Guo Ren","doi":"10.3967/bes2025.033","DOIUrl":"https://doi.org/10.3967/bes2025.033","url":null,"abstract":"","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"38 4","pages":"519-524"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144055216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kitchen Ventilation Attenuate the Association of Solid Fuel Use with Sarcopenia: A Cross-Sectional and Prospective Study.","authors":"Ying Hao Yuchi, Wei Liao, Jia Qiu, Rui Ying Li, Ning Kang, Xiao Tian Liu, Wen Qian Huo, Zhen Xing Mao, Jian Hou, Lei Zhang, Chong Jian Wang","doi":"10.3967/bes2025.031","DOIUrl":"https://doi.org/10.3967/bes2025.031","url":null,"abstract":"","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"38 4","pages":"511-515"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144047988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Efficacy of BMSC Transplantation <i>via</i> Various Pathways for Treating Cholestatic Liver Fibrosis in Mice.","authors":"Jun Jie Ren, Zi Xu Li, Xin Rui Shi, Ting Ting Lyu, Xiao Nan Li, Min Ge, Qi Zhi Shuai, Ting Juan Huang","doi":"10.3967/bes2024.180","DOIUrl":"https://doi.org/10.3967/bes2024.180","url":null,"abstract":"<p><strong>Objective: </strong>To compare the therapeutic efficacy of portal and tail vein transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) against cholestatic liver fibrosis in mice.</p><p><strong>Methods: </strong>BMSCs were isolated and co-cultured with starvation-activated hepatic stellate cells (HSCs). HSC activation markers were identified using immunofluorescence and qRT-PCR. BMSCs were injected into the liver tissues of bile duct ligation (BDL) mice <i>via</i> the tail and portal veins. Histomorphology, liver function, inflammatory cytokines, and the expression of key proteins were all determined in the liver tissues.</p><p><strong>Results: </strong>BMSCs inhibited HSC activation by reducing α-SMA and collagen I expression. Compared to tail vein injection, DIL-labeled BMSCs injected through the portal vein maintained a high homing rate in the liver. Moreover, BMSCs transplanted through the portal vein resulted in greater improvement in liver color, hardness, and gallbladder size than did those transplanted through the tail vein. Furthermore, BMSCs injected by portal vein, but not tail vein, markedly ameliorated liver function, reduced the secretion of inflammatory cytokines, including TNF-α, IL-6, and IL-1β, and decreased α-SMA + hepatic stellate cell (HSC) activation and collagen fiber formation.</p><p><strong>Conclusion: </strong>The therapeutic effect of BMSCs on cholestatic liver fibrosis in mice <i>via</i> portal vein transplantation was superior to that of tail vein transplantation. This comparative study provides reference information for further BMSC studies focused on clinical cholestatic liver diseases.</p>","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"38 4","pages":"447-458"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Threshold-Effect Associations of Serum 25-hydroxyvitamin D on Bone Turnover Markers and GC rs2282679 Variants in Chinese Women of Childbearing Age.","authors":"Xiao Yun Shan, Yu Ting Li, Xia Yu Zhao, Yi Chun Hu, Si Ran Li, Hui di Zhang, Yang Cao, Rui Wang, Li Chen Yang","doi":"10.3967/bes2024.151","DOIUrl":"https://doi.org/10.3967/bes2024.151","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate possible serum 25-hydroxyvitamin D [25(OH)D] cutoffs for the associations between 25(OH)D and Bone turnover markers (BTMs), and how GC gene variation influences such cutoffs in Chinese women of childbearing age.</p><p><strong>Methods: </strong>In total, 1,505 non-pregnant or non-lactating women (18-45 years) were recruited from the 2015 Chinese Adult Chronic Disease and Nutrition Surveillance. Serum 25(OH)D, osteocalcin (OC), procollagen type 1 N-terminal propeptide (P1NP), β-CrossLaps of type 1 collagen containing cross-linked C-telopeptide (β-CTX), and single nucleotide polymorphisms were determined. Locally weighted regression and smoothing scatterplot and segmented regression were performed to estimate the 25(OH)D thresholds.</p><p><strong>Results: </strong>The median serum 25(OH)D was 16.63 (11.96-22.55) ng/mL and the prevalence of low serum 25(OH)D (< 12 ng/mL) was 25.2%. Women with the lowest 25(OH)D had the highest β-CTX. After adjustment for the confounders, 25(OH)D cutoffs for OC [14.04 (12.84-15.23) ng/mL], β-CTX [13.94 (12.49-15.39) ng/mL], and P1NP [13.87 (12.37-15.37) ng/mL] in the whole population, cutoffs for OC [12.30 (10.68-13.91) ng/mL], β-CTX [12.23 (10.22-14.23) ng/mL], and P1NP [11.85 (10.40-13.31) ng/mL] in women with the GC rs2282679 G allele, and cutoffs for OC [12.75 (11.81-13.68) ng/mL], β-CTX [13.05 (11.78-14.32) ng/mL], and P1NP [12.81 (11.57-14.06) ng/mL] in women with the GC rs2282679 T allele, were observed. Below these cutoffs, BTMs were negatively associated with 25(OH)D, while above these cutoffs, BTMs plateaued.</p><p><strong>Conclusion: </strong>In Chinese women of childbearing age, there were thresholds effect of serum 25(OH)D concentrations on BTMs. The results indicated that serum 25(OH)D concentrations < 13.87 ng/mL in this population had adverse influences on maintaining bone remodeling. BTMs were suppressed at a relatively lower serum 25(OH)D in women with the GC rs2282679 G allele compared with those with the T allele.</p>","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"38 4","pages":"433-446"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Gold Nanorods Functionalized by Nucleic Acid Nanostructures Carrying Doxorubicin in Synergistic Anti-Cancer Therapy.","authors":"Hao Wu, Huang Shui Ma, Xing Han Wu, Qiang Sun, Lin Feng, Rui Fang Jiang, Yan Hong Li, Quan Shi","doi":"10.3967/bes2024.152","DOIUrl":"https://doi.org/10.3967/bes2024.152","url":null,"abstract":"<p><strong>Objective: </strong>Cancer remains a significant global health challenge, necessitating the development of effective treatment approaches. Developing synergistic therapy can provide a highly promising strategy for anti-cancer treatment through combining the benefits of various mechanisms.</p><p><strong>Methods: </strong>In this study, we developed a synergistic strategy for chemo-photothermal therapy by constructing nanocomposites using gold nanorods (GNRs) and tetrahedral framework nucleic acids (tFNA) loaded with the anti-tumor drug doxorubicin (DOX).</p><p><strong>Results: </strong>Our <i>in vitro</i> studies have systematically clarified the anti-cancer behaviors of tFNA-DOX@GNR nanocomposites, characterized by their enhanced cellular uptake and proficient lysosomal escape capabilities. It was found that the key role of tFNA-DOX@GNR nanocomposites in tumor ablation is primarily due to their capacity to induce cytotoxicity in tumor cells <i>via</i> a photothermal effect, which generates instantaneous high temperatures. This mechanism introduces various responses in tumor cells, facilitated by the thermal effect and the integrated chemotherapeutic action of DOX. These reactions include the induction of endoplasmic reticulum stress, characterized by elevated reactive oxygen species levels, the promotion of apoptotic cell death, and the suppression of tumor cell proliferation.</p><p><strong>Conclusion: </strong>This work exhibits the potential of synergistic therapy utilizing nanocomposites for cancer treatment and offers a promising avenue for future therapeutic strategies.</p>","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"38 4","pages":"403-415"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Validation of Machine Learning Models for Lung Cancer Risk Prediction in High-Risk Population: A Retrospective Cohort Study.","authors":"Yu Su, Hao Ran Zhan, Shang Yao Li, Yi Tong Lu, Ru Huan Ma, Hai Fang, Ting Ting Xu, Yu Tian","doi":"10.3967/bes2025.038","DOIUrl":"https://doi.org/10.3967/bes2025.038","url":null,"abstract":"","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"38 4","pages":"501-505"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144045966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}