Asian journal of andrology最新文献_第5页

A novel homozygous mutation of CFAP300 identified in a Chinese patient with primary ciliary dyskinesia and infertility. 在一名患有原发性睫状肌运动障碍和不孕症的中国患者身上发现 CFAP300 的新型同源突变。

Asian journal of andrology Pub Date : 2025-01-01 Epub Date: 2024-09-10 DOI: 10.4103/aja202477

Zheng Zhou, Qi Qi, Wen-Hua Wang, Jie Dong, Juan-Juan Xu, Yu-Ming Feng, Zhi-Chuan Zou, Li Chen, Jin-Zhao Ma, Bing Yao

{"title":"A novel homozygous mutation of CFAP300 identified in a Chinese patient with primary ciliary dyskinesia and infertility.","authors":"Zheng Zhou, Qi Qi, Wen-Hua Wang, Jie Dong, Juan-Juan Xu, Yu-Ming Feng, Zhi-Chuan Zou, Li Chen, Jin-Zhao Ma, Bing Yao","doi":"10.4103/aja202477","DOIUrl":"10.4103/aja202477","url":null,"abstract":"Abstract: Primary ciliary dyskinesia (PCD) is a clinically rare, genetically and phenotypically heterogeneous condition characterized by chronic respiratory tract infections, male infertility, tympanitis, and laterality abnormalities. PCD is typically resulted from variants in genes encoding assembly or structural proteins that are indispensable for the movement of motile cilia. Here, we identified a novel nonsense mutation, c.466G>T, in cilia- and flagella-associated protein 300 ( CFAP300 ) resulting in a stop codon (p.Glu156*) through whole-exome sequencing (WES). The proband had a PCD phenotype with laterality defects and immotile sperm flagella displaying a combined loss of the inner dynein arm (IDA) and outer dynein arm (ODA). Bioinformatic programs predicted that the mutation is deleterious. Successful pregnancy was achieved through intracytoplasmic sperm injection (ICSI). Our results expand the spectrum of CFAP300 variants in PCD and provide reproductive guidance for infertile couples suffering from PCD caused by them.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":"113-119"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142303268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of genome tagging technology in elucidating the function of sperm-specific protein 411 (Ssp411). 应用基因组标记技术阐明精子特异性蛋白411（Ssp411）的功能。

Asian journal of andrology Pub Date : 2025-01-01 Epub Date: 2024-08-02 DOI: 10.4103/aja202442

Xue-Hai Zhou, Min-Min Hua, Jia-Nan Tang, Bang-Guo Wu, Xue-Mei Wang, Chang-Gen Shi, Yang Yang, Jun Wu, Bin Wu, Bao-Li Zhang, Yi-Si Sun, Tian-Cheng Zhang, Hui-Juan Shi

{"title":"Application of genome tagging technology in elucidating the function of sperm-specific protein 411 (Ssp411).","authors":"Xue-Hai Zhou, Min-Min Hua, Jia-Nan Tang, Bang-Guo Wu, Xue-Mei Wang, Chang-Gen Shi, Yang Yang, Jun Wu, Bin Wu, Bao-Li Zhang, Yi-Si Sun, Tian-Cheng Zhang, Hui-Juan Shi","doi":"10.4103/aja202442","DOIUrl":"10.4103/aja202442","url":null,"abstract":"Abstract: The genome tagging project (GTP) plays a pivotal role in addressing a critical gap in the understanding of protein functions. Within this framework, we successfully generated a human influenza hemagglutinin-tagged sperm-specific protein 411 (HA-tagged Ssp411) mouse model. This model is instrumental in probing the expression and function of Ssp411. Our research revealed that Ssp411 is expressed in the round spermatids, elongating spermatids, elongated spermatids, and epididymal spermatozoa. The comprehensive examination of the distribution of Ssp411 in these germ cells offers new perspectives on its involvement in spermiogenesis. Nevertheless, rigorous further inquiry is imperative to elucidate the precise mechanistic underpinnings of these functions. Ssp411 is not detectable in metaphase II (MII) oocytes, zygotes, or 2-cell stage embryos, highlighting its intricate role in early embryonic development. These findings not only advance our understanding of the role of Ssp411 in reproductive physiology but also significantly contribute to the overarching goals of the GTP, fostering groundbreaking advancements in the fields of spermiogenesis and reproductive biology.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":"120-128"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141876950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predictability of varicocele repair success: preliminary results of a machine learning-based approach. 精索静脉曲张修复成功率的可预测性：基于机器学习方法的初步结果。

Asian journal of andrology Pub Date : 2025-01-01 Epub Date: 2024-08-09 DOI: 10.4103/aja202438

Andrea Crafa, Marco Russo, Rossella Cannarella, Murat Gül, Michele Compagnone, Laura M Mongioì, Vittorio Cannarella, Rosita A Condorelli, Sandro La Vignera, Aldo E Calogero

{"title":"Predictability of varicocele repair success: preliminary results of a machine learning-based approach.","authors":"Andrea Crafa, Marco Russo, Rossella Cannarella, Murat Gül, Michele Compagnone, Laura M Mongioì, Vittorio Cannarella, Rosita A Condorelli, Sandro La Vignera, Aldo E Calogero","doi":"10.4103/aja202438","DOIUrl":"10.4103/aja202438","url":null,"abstract":"Abstract: Varicocele is a prevalent condition in the infertile male population. However, to date, which patients may benefit most from varicocele repair is still a matter of debate. The purpose of this study was to evaluate whether certain preintervention sperm parameters are predictive of successful varicocele repair, defined as an improvement in total motile sperm count (TMSC). We performed a retrospective study on 111 patients with varicocele who had undergone varicocele repair, collected from the Department of Endocrinology, Metabolic Diseases and Nutrition, University of Catania (Catania, Italy), and the Unit of Urology at the Selcuk University School of Medicine (Konya, Türkiye). The predictive analysis was conducted through the use of the Brain Project, an innovative tool that allows a complete and totally unbiased search of mathematical expressions that relate the object of study to the various parameters available. Varicocele repair was considered successful when TMSC increased by at least 50% of the preintervention value. For patients with preintervention TMSC below 5 × 10 6 , improvement was considered clinically relevant when the increase exceeded 50% and the absolute TMSC value was >5 × 10 6 . From the preintervention TMSC alone, we found a model that predicts patients who appear to benefit little from varicocele repair with a sensitivity of 50.0% and a specificity of 81.8%. Varicocele grade and serum follicle-stimulating hormone (FSH) levels did not play a predictive role, but it should be noted that all patients enrolled in this study were selected with intermediate- or high-grade varicocele and normal FSH levels. In conclusion, preintervention TMSC is predictive of the success of varicocele repair in terms of TMSC improvement in patients with intermediate- or high-grade varicoceles and normal FSH levels.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":"52-58"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784956/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141908561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro effects of antidepressants on human sperm function. 抗抑郁药对人类精子功能的体外影响。

Asian journal of andrology Pub Date : 2025-01-01 Epub Date: 2024-08-13 DOI: 10.4103/aja202459

Rita António Santos, Ana Paula Sousa, Teresa Almeida-Santos, João Ramalho-Santos, Renata Santos Tavares

{"title":"In vitro effects of antidepressants on human sperm function.","authors":"Rita António Santos, Ana Paula Sousa, Teresa Almeida-Santos, João Ramalho-Santos, Renata Santos Tavares","doi":"10.4103/aja202459","DOIUrl":"10.4103/aja202459","url":null,"abstract":"Abstract: Depression currently affects about 280 million people worldwide and its prevalence has been increasing dramatically, especially among the young and people of reproductive age, which consequently leads to an increase in antidepressant consumption. Antidepressants are associated with sexual dysfunction in both men and women; however, their role in male fertility has been scarcely studied. Fluoxetine and sertraline, two serotonin reuptake inhibitors (SSRIs), are among the most prescribed antidepressants worldwide. To determine their possible effects, human sperm cells were exposed to either sertraline or fluoxetine at concentrations previously found in blood and seminal fluid of patients undergoing treatment. Spermatozoa were incubated for up to 24 h at 37°C and 5% CO 2 , and important functional parameters such as sperm motility, viability, mitochondrial membrane potential, cellular reactive oxygen species (ROS) production, chromatin/DNA integrity, acrosome status, and tyrosine phosphorylation were assessed. At low levels, fluoxetine consistently decreased progressive motility throughout time while promoting fluctuations in ROS levels and sperm capacitation. Nevertheless, it did not affect viability, mitochondrial membrane potential, acrosome reaction nor chromatin/DNA integrity. Sertraline, on the other hand, had little to nonsignificant impact at low doses, but affected almost all tested parameters at supratherapeutic concentrations. Altogether, our results suggest that both antidepressants may impair sperm function, possibly through different mechanisms of action, but fluoxetine is the only exhibiting mild negative effects at doses found in vivo .","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":"30-36"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141972457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Asian journal of andrology Pub Date : 2025-01-01 Epub Date: 2024-08-09 DOI: 10.4103/aja202449

Zhi-Yi Zhao, Yong Siow, Ling-Yun Liu, Xian Li, Hong-Liang Wang, Zhen-Min Lei

{"title":"The SPARC-related modular calcium binding 1 ( Smoc1 ) regulated by androgen is required for mouse gubernaculum development and testicular descent.","authors":"Zhi-Yi Zhao, Yong Siow, Ling-Yun Liu, Xian Li, Hong-Liang Wang, Zhen-Min Lei","doi":"10.4103/aja202449","DOIUrl":"10.4103/aja202449","url":null,"abstract":"Abstract: Testicular descent occurs in two consecutive stages: the transabdominal stage and the inguinoscrotal stage. Androgens play a crucial role in the second stage by influencing the development of the gubernaculum, a structure that pulls the testis into the scrotum. However, the mechanisms of androgen actions underlying many of the processes associated with gubernaculum development have not been fully elucidated. To identify the androgen-regulated genes, we conducted large-scale gene expression analyses on the gubernaculum harvested from luteinizing hormone/choriogonadotropin receptor knockout ( Lhcgr KO) mice, an animal model of inguinoscrotal testis maldescent resulting from androgen deficiency. We found that the expression of secreted protein acidic and rich in cysteine (SPARC)-related modular calcium binding 1 ( Smoc1 ) was the most severely suppressed at both the transcript and protein levels, while its expression was the most dramatically induced by testosterone administration in the gubernacula of Lhcgr KO mice. The upregulation of Smoc1 expression by testosterone was curtailed by the addition of an androgen receptor antagonist, flutamide. In addition, in vitro studies demonstrated that SMOC1 modestly but significantly promoted the proliferation of gubernacular cells. In the cultures of myogenic differentiation medium, both testosterone and SMOC1 enhanced the expression of myogenic regulatory factors such as paired box 7 ( Pax7 ) and myogenic factor 5 ( Myf5 ). After short-interfering RNA-mediated knocking down of Smoc1 , the expression of Pax7 and Myf5 diminished, and testosterone alone did not recover, but additional SMOC1 did. These observations indicate that SMOC1 is pivotal in mediating androgen action to regulate gubernaculum development during inguinoscrotal testicular descent.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":"44-51"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784950/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141908564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peripheral platelet count is a diagnostic marker for predicting the risk of rapid ejaculation: findings from a pilot study in rats. 外周血小板计数是预测快速射精风险的诊断指标：大鼠试验研究的结果。

Asian journal of andrology Pub Date : 2025-01-01 Epub Date: 2024-08-02 DOI: 10.4103/aja202447

Yuan-Yuan Huang, Nan Ye, Dang-Wei Peng, Guang-Yuan Li, Xian-Sheng Zhang

{"title":"Peripheral platelet count is a diagnostic marker for predicting the risk of rapid ejaculation: findings from a pilot study in rats.","authors":"Yuan-Yuan Huang, Nan Ye, Dang-Wei Peng, Guang-Yuan Li, Xian-Sheng Zhang","doi":"10.4103/aja202447","DOIUrl":"10.4103/aja202447","url":null,"abstract":"Abstract: Parameters of peripheral blood cell have been shown as the potential predictors of erectile dysfunction (ED). To investigate the clinical significance of hematological parameters for predicting the risk of rapid ejaculation, we established a rat copulatory model on the basis of ejaculation distribution theory. Blood samples from different ejaculatory groups were collected for peripheral blood cell counts and serum serotonin (5-HT) tests. Meanwhile, the relationship between hematological parameters and ejaculatory behaviors was assessed. Final analysis included 11 rapid ejaculators, 10 normal ejaculators, and 10 sluggish ejaculators whose complete data were available. The platelet (PLT) count in rapid ejaculators was significantly lower than that in normal and sluggish ejaculators, whereas the platelet distribution width (PDW) and mean platelet volume (MPV) were significantly greater in rapid ejaculators. Multivariate logistic regression analysis and receiver operating characteristic (ROC) curve analysis showed that the PLT was an independent protective factor for rapid ejaculation. Meanwhile, rapid ejaculators were found to have the lowest serum 5-HT compared to normal and sluggish ejaculators ( P < 0.001). Furthermore, there was a positive correlation between the PLT and serum 5-HT ( r = 0.662, P < 0.001), indicating that the PLT could indirectly reflect the serum 5-HT concentration. In addition, we assessed the association between the PLT and ejaculatory parameters. There was a negative correlation between ejaculation frequency (EF) and the PLT ( r = -0.595, P < 0.001), whereas there was a positive correlation between ejaculation latency (EL) and the PLT ( r = 0.740, P < 0.001). This study indicated that the PLT might be a useful and convenient diagnostic marker for predicting the risk of rapid ejaculation.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":"129-134"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784955/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141876951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Delayed covering causes the accumulation of motile sperm, leading to overestimation of sperm concentration and motility with a Makler counting chamber. 延迟覆盖会导致运动精子的积累，从而导致马克勒计数室对精子浓度和运动能力的高估。

Asian journal of andrology Pub Date : 2025-01-01 Epub Date: 2024-09-03 DOI: 10.4103/aja202474

Lin Yu, Qing-Yuan Cheng, Ye-Lin Jia, Yan Zheng, Ting-Ting Yang, Ying-Bi Wu, Fu-Ping Li

{"title":"Delayed covering causes the accumulation of motile sperm, leading to overestimation of sperm concentration and motility with a Makler counting chamber.","authors":"Lin Yu, Qing-Yuan Cheng, Ye-Lin Jia, Yan Zheng, Ting-Ting Yang, Ying-Bi Wu, Fu-Ping Li","doi":"10.4103/aja202474","DOIUrl":"10.4103/aja202474","url":null,"abstract":"Abstract: According to the World Health Organization (WHO) manual, sperm concentration should be measured using an improved Neubauer hemocytometer, while sperm motility should be measured by manual assessment. However, in China, thousands of laboratories do not use the improved Neubauer hemocytometer or method; instead, the Makler counting chamber is one of the most widely used chambers. To study sources of error that could impact the measurement of the apparent concentration and motility of sperm using the Makler counting chamber and to verify its accuracy for clinical application, 67 semen samples from patients attending the Department of Andrology, West China Second University Hospital, Sichuan University (Chengdu, China) between 13 September 2023 and 27 September 2023, were included. Compared with applying the cover glass immediately, delaying the application of the cover glass for 5 s, 10 s, and 30 s resulted in average increases in the sperm concentration of 30.3%, 74.1%, and 107.5%, respectively (all P < 0.0001) and in the progressive motility (PR) of 17.7%, 30.8%, and 39.6%, respectively (all P < 0.0001). However, when the semen specimens were fixed with formaldehyde, a delay in the application of the cover glass for 5 s, 10 s, and 30 s resulted in an average increase in the sperm concentration of 6.7%, 10.8%, and 14.6%, respectively, compared with immediate application of the cover glass. The accumulation of motile sperm due to delays in the application of the cover glass is a significant source of error with the Makler counting chamber and should be avoided.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":"59-64"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784962/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142121312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A behind-the-scenes role of BDNF in the survival and differentiation of spermatogonia. BDNF在精原细胞存活和分化过程中的幕后作用

Asian journal of andrology Pub Date : 2025-01-01 Epub Date: 2024-08-13 DOI: 10.4103/aja202457

Shin-Ichi Tomizawa, Kazushige Kuroha, Michio Ono, Kuniko Nakajima, Kazuyuki Ohbo

{"title":"A behind-the-scenes role of BDNF in the survival and differentiation of spermatogonia.","authors":"Shin-Ichi Tomizawa, Kazushige Kuroha, Michio Ono, Kuniko Nakajima, Kazuyuki Ohbo","doi":"10.4103/aja202457","DOIUrl":"10.4103/aja202457","url":null,"abstract":"Abstract: Mouse spermatogenesis entails the maintenance and self-renewal of spermatogonial stem cells (SSCs), which require a complex web-like signaling network transduced by various cytokines. Although brain-derived neurotrophic factor (BDNF) is expressed in Sertoli cells in the testis, and its receptor tropomyosin receptor kinase B (TrkB) is expressed in the spermatogonial population containing SSCs, potential functions of BDNF for spermatogenesis have not been uncovered. Here, we generate BDNF conditional knockout mice and find that BDNF is dispensable for in vivo spermatogenesis and fertility. However, in vitro , we reveal that BDNF -deficient germline stem cells (GSCs) exhibit growth potential not only in the absence of glial cell line-derived neurotrophic factor (GDNF), a master regulator for GSC proliferation, but also in the absence of other factors, including epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), and insulin. GSCs grown without these factors are prone to differentiation, yet they maintain expression of promyelocytic leukemia zinc finger ( Plzf ), an undifferentiated spermatogonial marker. Inhibition of phosphoinositide 3-kinase (PI3K), mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK), and Src pathways all interfere with the growth of BDNF-deficient GSCs. Thus, our findings suggest a role for BDNF in maintaining the undifferentiated state of spermatogonia, particularly in situations where there is a shortage of growth factors.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":"37-43"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142037990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Divergences in neuroendocrine prostate cancer frequency as recognized by anatomic pathologists, clinicians, and basic scientists. 解剖病理学家、临床医生和基础科学家认识到的神经内分泌前列腺癌频率差异。

Asian journal of andrology Pub Date : 2025-01-01 Epub Date: 2024-08-20 DOI: 10.4103/aja202467

Kenneth A Iczkowski, Mamta Parikh

引用次数: 0

Sperm RNA quantity and PRM1, PRM2 , and TH2B transcript levels reflect sperm characteristics and early embryonic development. 精子 RNA 数量以及 PRM1、PRM2 和 TH2B 转录本水平反映了精子的特征和早期胚胎发育。

Asian journal of andrology Pub Date : 2025-01-01 Epub Date: 2024-08-27 DOI: 10.4103/aja202452

Bilge Ozsait-Selcuk, Sibel Bulgurcuoglu-Kuran, Dilek Sever-Kaya, Neslihan Coban, Gulsen Aktan, Ates Kadioglu

{"title":"Sperm RNA quantity and PRM1, PRM2 , and TH2B transcript levels reflect sperm characteristics and early embryonic development.","authors":"Bilge Ozsait-Selcuk, Sibel Bulgurcuoglu-Kuran, Dilek Sever-Kaya, Neslihan Coban, Gulsen Aktan, Ates Kadioglu","doi":"10.4103/aja202452","DOIUrl":"10.4103/aja202452","url":null,"abstract":"Abstract: Spermatozoa have a highly complex RNA profile. Several of these transcripts are suggested as biomarkers for male infertility and contribute to early development. To analyze the differences between sperm RNA quantity and expression of protamine ( PRM1 and PRM2 ) and testis-specific histone 2B ( TH2B ) genes, spermatozoa from 33 patients who enrolled in assisted reproduction treatment (ART) program were analyzed. Sperm RNA of teratozoospermic (T), oligoteratozoospermic (OT), and normozoospermic (N) samples was extracted, and the differences in transcript levels among the study groups were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). The correlations of total RNA per spermatozoon and the expression of the transcripts were evaluated in relation to sperm characteristics and preimplantation embryo development. The mean (±standard deviation) RNA amount per spermatozoon was 28.48 (±23.03) femtogram in the overall group and was significantly higher in the OT group than that in N and T groups. Total sperm RNA and gene expression of PRM1 and PRM2 genes were related to preimplantation embryo development and developmental arrest. Specific sperm characteristics were correlated with the expressions of PRM1 , PRM2 , or TH2B genes. We conclude that the sperm RNA amount and composition are important factors and might influence early embryonic development and also differ in different cases of male infertility.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":"76-83"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142074821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0