Asian journal of andrology最新文献_第5页

Relationship between plasma homocysteine and clinical grading of varicocele. 血浆同型半胱氨酸与精索静脉曲张临床分级的关系。

Asian journal of andrology Pub Date : 2025-04-25 DOI: 10.4103/aja202511

Li-Hong Wang, Lei Zheng, Hui Jiang, Tao Jiang

{"title":"Relationship between plasma homocysteine and clinical grading of varicocele.","authors":"Li-Hong Wang, Lei Zheng, Hui Jiang, Tao Jiang","doi":"10.4103/aja202511","DOIUrl":"https://doi.org/10.4103/aja202511","url":null,"abstract":"Abstract: This study aims to explore the correlation between plasma homocysteine (Hcy) levels and the clinical grading of varicocele (VC) when analyzing the potential pathogenesis of endothelial cells injury by Hcy. A total of 184 VC patients, aged 18-46 years, were included in this study. These patients visited The Second Hospital of Dalian Medical University (Dalian, China), between January 2022 and September 2024. Patients were divided into three groups based on clinical grading: Group A (59 cases, Grade I), Group B (28 cases, Grade II), and Group C (97 cases, Grade III). Additionally, 120 individuals with normal fertility test results during the same period were selected as the control group. Routine blood and biochemical indices were collected from the patients. Differences in clinical indices between groups were compared, and univariate and multivariate linear regression analyses were performed to identify factors associated with clinical grading. The results showed that the median Hcy levels in the control group and in patients with Grade I, II, and III VC were 9.56 (interquartile range [IQR]: 8.66, 14.02) µmol l-1, 11.28 (IQR: 9.71, 14.55) µmol l-1, 11.84 (IQR: 10.14, 15.60) µmol l-1, and 12.27 (IQR: 9.52, 15.40) µmol l-1, respectively. The differences between the four groups were statistically significant (χ2 = 12.41, P = 0.006). Multivariate regression analysis indicated that Hcy is a factor associated with the clinical grading of VC (t = 2.53, P = 0.013). Hcy is associated with the clinical grading and may have clinical value in assessing severity of VC.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144060849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sexual activity in men undergoing ADT for localized prostate cancer: a narrative literature review. 局限性前列腺癌接受ADT治疗的男性的性活动：一篇叙述性文献综述。

Asian journal of andrology Pub Date : 2025-04-25 DOI: 10.4103/aja20253

Laeticia Creidy, Daniel Taussky, Frederic Pouliot, Fred Saad, Fabian Falkenbach

{"title":"Sexual activity in men undergoing ADT for localized prostate cancer: a narrative literature review.","authors":"Laeticia Creidy, Daniel Taussky, Frederic Pouliot, Fred Saad, Fabian Falkenbach","doi":"10.4103/aja20253","DOIUrl":"https://doi.org/10.4103/aja20253","url":null,"abstract":"Abstract: Androgen deprivation therapy (ADT) can negatively affect sexual function, and only a minority of patients report sexual activity. We reviewed the existing literature regarding the proportion of men who remained sexually active during and after ADT. The PubMed database was searched for studies published over the past 20 years. We selected and reviewed randomized clinical trials that provided sexual function data at baseline and during and after ADT. The primary outcome measure was the sexual function. Studies assessed sexual function using quality of life patient-reported outcome measures, which included sexual potency/activity evaluation. Information from 2947 patients was analyzed in this review. The median age of patients was 70 years. At baseline, a median of 49.9% (95% confidence interval [CI]: 49.1%-50.7%) of the patients reported being sexually active. At 6 months, 12 months, and 2 years or later of ADT treatment, a median of 10.3% (95% CI: 10.2%-10.5%), 8.9% (95% CI: 8.6%-9.2%), and 8.3% (95% CI: 8.2%-8.5%) of the patients reported being sexually active, respectively. Considering that half of the patients were sexually active at baseline, it seems probable that more than 10% of the patients who were sexually active before starting ADT remained sexually active when undergoing ADT. In conclusion, despite the common belief that ADT eliminates sexual activity, this analysis found that approximately 1 in 10 men are sexually active when on ADT, and this proportion is likely increased in men who are sexually active before starting ADT. Attention to sexual activity should not be dismissed in men on ADT.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144061730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic systems in testicular in vitro culture: a powerful model tool for spermatogenesis and reprotoxicity studies. 睾丸体外培养中的微流体系统：精子发生和生殖毒性研究的强大模型工具。

Asian journal of andrology Pub Date : 2025-04-22 DOI: 10.4103/aja20254

Botho Maximilian Schneider, Hande Irem Hamurcu, Andrea Salzbrunn, Kathrein von Kopylow

{"title":"Microfluidic systems in testicular in vitro culture: a powerful model tool for spermatogenesis and reprotoxicity studies.","authors":"Botho Maximilian Schneider, Hande Irem Hamurcu, Andrea Salzbrunn, Kathrein von Kopylow","doi":"10.4103/aja20254","DOIUrl":"https://doi.org/10.4103/aja20254","url":null,"abstract":"Abstract: As prepubertal boys do not yet produce spermatozoa, they cannot rely on sperm cryopreservation for fertility preservation before gonadotoxic therapy, such as high-dose alkylating agents or radiotherapy in the case of childhood cancers. According to the current guidelines, cryopreservation of testicular biopsies containing spermatogonial stem cells (SSCs) may be proposed to high-risk patients for potential later therapeutic use to fulfill the patients' wish for a biological child. One promising technique for human in vitro spermatogenesis and in vitro propagation of human SSCs is microfluidic (MF) culture, in which cells or tissues are subjected to a continuous flow of medium. This provides exact control over such parameters as nutrient content and gradients, as well as the removal of waste metabolites. While MF has been shown to maintain tissues and cell populations of organs for longer than conventional in vitro culture techniques, it has not been widely used for testicular in vitro culture. MF could advance human testicular in vitro culture and is also applicable to reprotoxicity studies. This review summarizes the findings and achievements of testis-on-chip (ToC) setups to date and discusses the benefits and limitations of these for spermatogenesis in vitro and toxicity assessment.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144058753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exogenous administration of heparin-binding epidermal growth factor-like growth factor improves erectile function in mice with bilateral cavernous nerve injury. 外源性肝素结合表皮生长因子样生长因子可改善双侧海绵状神经损伤小鼠的勃起功能。

Asian journal of andrology Pub Date : 2025-04-18 DOI: 10.4103/aja2024125

Minh Nhat Vo, Mi-Hye Kwon, Fang-Yuan Liu, Fitri Rahma Fridayana, Yan Huang, Soon-Sun Hong, Ju-Hee Kang, Guo Nan Yin, Ji-Kan Ryu

{"title":"Exogenous administration of heparin-binding epidermal growth factor-like growth factor improves erectile function in mice with bilateral cavernous nerve injury.","authors":"Minh Nhat Vo, Mi-Hye Kwon, Fang-Yuan Liu, Fitri Rahma Fridayana, Yan Huang, Soon-Sun Hong, Ju-Hee Kang, Guo Nan Yin, Ji-Kan Ryu","doi":"10.4103/aja2024125","DOIUrl":"https://doi.org/10.4103/aja2024125","url":null,"abstract":"Prostate cancer is the second most common malignancy and the sixth leading cause of cancer-related death in men worldwide. Radical prostatectomy (RP) is the standard treatment for localized prostate cancer, but the procedure often results in postoperative erectile dysfunction (ED). The poor efficacy of phosphodiesterase 5 inhibitors after surgery highlights the need to develop new therapies to enhance cavernous nerve regeneration and improve the erectile function of these patients. In the present study, we aimed to examine the potential of heparin-binding epidermal growth factor-like growth factor (HB-EGF) in preserving erectile function in cavernous nerve injury (CNI) mice. We found that HB-EGF expression was reduced significantly on the 1st day after CNI in penile tissue. Ex vivo and in vitro studies showed that HB-EGF promotes major pelvic ganglion neurite sprouting and neuro-2a (N2a) cell migration. In vivo studies showed that exogenous HB-EGF treatment significantly restored the erectile function of CNI mice to 86.9% of sham levels. Immunofluorescence staining showed that mural and neuronal cells were preserved by inducing cell proliferation and reducing apoptosis and reactive oxygen species production. Western blot analysis showed that HB-EGF upregulated protein kinase B and extracellular signal-regulated kinase activation and neurotrophic factor expression. Overall, HB-EGF is a major promising therapeutic agent for treating ED in postoperative RP.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144032098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements in penile lengthening techniques concurrent with penile prosthesis placement: a narrative review. 阴茎延长技术与阴茎假体放置的进展：叙述回顾。

Asian journal of andrology Pub Date : 2025-04-18 DOI: 10.4103/aja202512

Mattia Lo Re, Manuel Alonso Isa, Esther Garcia Rojo, Celeste Manfredi, Agustin Fraile Poblador, Marta Pezzoli, Andrea Cocci, Francesco Sessa, Andrea Minervini, Javier Romero-Otero

{"title":"Advancements in penile lengthening techniques concurrent with penile prosthesis placement: a narrative review.","authors":"Mattia Lo Re, Manuel Alonso Isa, Esther Garcia Rojo, Celeste Manfredi, Agustin Fraile Poblador, Marta Pezzoli, Andrea Cocci, Francesco Sessa, Andrea Minervini, Javier Romero-Otero","doi":"10.4103/aja202512","DOIUrl":"https://doi.org/10.4103/aja202512","url":null,"abstract":"Penile prosthetic devices stand as the foremost solution for medication-resistant erectile dysfunction. Frequently, medical conditions triggering erectile dysfunction also led to penile shortening, detrimentally impacting patients' quality of life. This narrative review aims to explore and evaluate the various techniques available for penile lengthening that can be performed concurrently with inflatable penile prosthesis (IPP) insertion. We conducted a comprehensive examination of the literature, utilizing PubMed focusing on key terms such as \"penile prosthesis corporal length\", \"inflatable penile prosthesis and short penis\", and \"buried penis\". The review identified several advanced methodologies for preserving and enhancing penile length during IPP insertion. These techniques include subcoronal IPP insertion, sliding technique, modified sliding technique, multiple slice technique, and circumferential incision with grafting. Supplementary procedures aimed at improving the perception of increased length involve ventral phalloplasty and suprapubic lipectomy. It is worth noting that the maximum length gain seems to be constrained by the length of the neurovascular bundles. In summary, the evidence available in the literature is limited due to the short of case series reported, lack of randomized controlled trials, and heterogeneity of the studies. The literature suggests that for carefully selected patients, surgical penile lengthening procedures performed concurrently with IPP insertion emerge as effective treatments for individuals grappling with penile shortening and severe erectile dysfunction. Well-designed, larger studies are needed to establish the safety and efficacy of these procedures.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144048610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recurrent spontaneous miscarriages from sperm after ABVD chemotherapy in a patient with Hodgkin's lymphoma: sperm DNA and methylation profiling. 霍奇金淋巴瘤患者ABVD化疗后精子复发性自发流产：精子DNA和甲基化分析

Asian journal of andrology Pub Date : 2025-04-15 DOI: 10.4103/aja2024107

Gwendoline Lecuyer, Antoine D Rolland, Anne-Sophie Neyroud, Bertrand Evrard, Nathan Alary, Clemence Genthon, Nathalie Dejucq-Rainsford, Célia Ravel, Jessika Moreau, Nathalie Moinard, Mohamed Hadi Mohamed Abdelhamid, Christophe Klopp, Louis Bujan, Frédéric Chalmel

{"title":"Recurrent spontaneous miscarriages from sperm after ABVD chemotherapy in a patient with Hodgkin's lymphoma: sperm DNA and methylation profiling.","authors":"Gwendoline Lecuyer, Antoine D Rolland, Anne-Sophie Neyroud, Bertrand Evrard, Nathan Alary, Clemence Genthon, Nathalie Dejucq-Rainsford, Célia Ravel, Jessika Moreau, Nathalie Moinard, Mohamed Hadi Mohamed Abdelhamid, Christophe Klopp, Louis Bujan, Frédéric Chalmel","doi":"10.4103/aja2024107","DOIUrl":"https://doi.org/10.4103/aja2024107","url":null,"abstract":"Abstract: Lymphomas represent one of the most common malignant diseases in young men and an important issue is how treatments will affect their reproductive health. It has been hypothesized that chemotherapies, similarly to environmental chemicals, may alter the spermatogenic epigenome. Here, we report the genomic and epigenomic profiling of the sperm DNA from a 31-year-old Hodgkin lymphoma patient who faced recurrent spontaneous miscarriages in his couple 11-26 months after receiving chemotherapy with adriamycin, bleomycin, vinblastine, and dacarbazine (ABVD). In order to capture the potential deleterious impact of the ABVD treatment on mutational and methylation changes, we compared sperm DNA before and 26 months after chemotherapy with whole-genome sequencing (WGS) and reduced representation bisulfite sequencing (RRBS). The WGS analysis identified 403 variants following ABVD treatment, including 28 linked to genes crucial for embryogenesis. However, none were found in coding regions, indicating no impact of chemotherapy on protein function. The RRBS analysis identified 99 high-quality differentially methylated regions (hqDMRs) for which methylation status changed upon chemotherapy. Those hqDRMs were associated with 87 differentially methylated genes, among which 14 are known to be important or expressed during embryo development. While no variants were detected in coding regions, promoter regions of several genes potentially important for embryo development contained variants or displayed an altered methylated status. These might in turn modify the corresponding gene expression and thus affect their function during key stages of embryogenesis, leading to potential developmental disorders or miscarriages.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated seminal plasma metabolomics and lipidomics profiling highlight distinctive signature of varicocele patients with male infertility. 精浆代谢组学和脂质组学综合分析突出了精索静脉曲张患者男性不育症的独特特征。

Asian journal of andrology Pub Date : 2025-04-08 DOI: 10.4103/aja2024113

Jing-Di Zhang, Xiao-Gang Li, Rong-Rong Wang, Xin-Xin Feng, Si-Yu Wang, Hai Wang, Yu-Tao Wang, Hong-Jun Li, Yong-Zhe Li, Ye Guo

{"title":"Integrated seminal plasma metabolomics and lipidomics profiling highlight distinctive signature of varicocele patients with male infertility.","authors":"Jing-Di Zhang, Xiao-Gang Li, Rong-Rong Wang, Xin-Xin Feng, Si-Yu Wang, Hai Wang, Yu-Tao Wang, Hong-Jun Li, Yong-Zhe Li, Ye Guo","doi":"10.4103/aja2024113","DOIUrl":"https://doi.org/10.4103/aja2024113","url":null,"abstract":"Varicocele (VC) is a common cause of male infertility, yet there is a lack of molecular information for VC-associated male infertility. This study investigated alterations in the seminal plasma metabolomic and lipidomic profiles of infertile male VC patients. Twenty infertile males with VC and twenty-three age-matched healthy controls (HCs) were recruited from Peking Union Medical College Hospital (Beijing, China) between October 2019 and April 2021. Untargeted metabolite and lipid profiles from seminal plasma were analyzed using mass spectrometry. Four hundred and seventy-six metabolites and seventeen lipids were significantly different in infertile male VC patients compared to HCs. The top enriched pathways among these significantly different metabolites are protein digestion and absorption, aminoacyl-transfer RNA (tRNA) biosynthesis, and biosynthesis of amino acids. Different key lipid species, including triglyceride (TG), diacylglycerol (DG), ceramides (Cer), and phosphatidylserine (PS), varied between VC and HC groups. The distinct metabolites and lipids were moderately correlated. DL-3-phenyllactic acid is a potential diagnostic biomarker for VC-related male infertility (area under the curve [AUC] = 0.893), positively correlating with sperm count, concentration, and motility. Furthermore, DL-3-phenyllactic acid is the only metabolite shared by all four comparisons (VC vs HC, VC-induced oligoasthenospermia [OAS] vs VC-induced asthenospermia [AS], OAS vs HC, and AS vs HC). DL-3-phenyllactic acid significantly decreased in OAS than AS. Metabolite-targeting gene analysis revealed carbonic anhydrase 9 (CA9) might be the strongest candidate associated with the onset and severity of VC. The seminal plasma metabolite and lipid profiles of infertile males with VC differ significantly from those of HCs. DL-3-phenyllactic acid could be a promising biomarker.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143805081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Observation on the therapeutic effect of a modified Devine procedure with subcutaneous sliding fixation method for concealed penis. 改良Devine手术加皮下滑动固定治疗隐匿性阴茎的疗效观察。

Asian journal of andrology Pub Date : 2025-03-25 DOI: 10.4103/aja20251

Mohammed Abdulkarem Al-Qaisi, Hai-Fu Tian, Jia-Jin Feng, Ke-Ming Chen, Jin Zhang, Yun-Shang Tuo, Xue-Hao Wang, Bin-Cheng Huang, Muhammad Arslan Ul Hassan, Rui He, Guang-Yong Li

{"title":"Observation on the therapeutic effect of a modified Devine procedure with subcutaneous sliding fixation method for concealed penis.","authors":"Mohammed Abdulkarem Al-Qaisi, Hai-Fu Tian, Jia-Jin Feng, Ke-Ming Chen, Jin Zhang, Yun-Shang Tuo, Xue-Hao Wang, Bin-Cheng Huang, Muhammad Arslan Ul Hassan, Rui He, Guang-Yong Li","doi":"10.4103/aja20251","DOIUrl":"https://doi.org/10.4103/aja20251","url":null,"abstract":"To evaluate the therapeutic effect of a modified Devine procedure with a subcutaneous sliding fixation method for the treatment of congenital concealed penis, we retrospectively selected 45 patients with congenital concealed penises who were admitted to General Hospital of Ningxia Medical University (Yinchuan, China) between September 2020 and November 2023. In all cases, the penis was observed to be short, and retracting the skin at the base revealed a normal penile body, which immediately returned to its original position upon release. All patients underwent the modified Devine procedure with subcutaneous sliding fixation and completed a 12-week postoperative follow-up. A statistically significant increase in penile length was observed postoperatively, with the median length increasing from 4.0 (interquartile range [IQR]: 3.5-4.8; 95% confidence interval [CI]: 3.9-4.4) cm to 8.0 (IQR: 7.8-8.0; 95% CI: 7.7-7.9) cm, with P < 0.001. The parents were satisfied with the outcomes, including increased penile length, improved hygiene, and enhanced esthetics. Except for mild foreskin edema in all cases, no complications (such as infections, skin necrosis, or penile retraction) were observed. The edema was resolved within 4 weeks after the operation. This study demonstrates that the modified Devine procedure utilizing the subcutaneous sliding fixation method yields excellent outcomes with minimal postoperative complications, reduced penile retraction, and high satisfaction rates among patients and their families.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143702496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeted gene silencing in mouse testicular Sertoli and Leydig cells using adeno-associated virus vectors. 利用腺相关病毒载体对小鼠睾丸支持细胞和间质细胞进行靶向基因沉默。

Asian journal of andrology Pub Date : 2025-03-21 DOI: 10.4103/aja2024120

Jing Pang, Mao-Xing Xu, Xiao-Yu Wang, Xu Feng, Yi-Man Duan, Xiao-Yan Zheng, Yu-Qian Chen, Wen Yin, Ying Liu, Ju-Xue Li

{"title":"Targeted gene silencing in mouse testicular Sertoli and Leydig cells using adeno-associated virus vectors.","authors":"Jing Pang, Mao-Xing Xu, Xiao-Yu Wang, Xu Feng, Yi-Man Duan, Xiao-Yan Zheng, Yu-Qian Chen, Wen Yin, Ying Liu, Ju-Xue Li","doi":"10.4103/aja2024120","DOIUrl":"https://doi.org/10.4103/aja2024120","url":null,"abstract":"Abstract: Researchers commonly use cyclization recombination enzyme/locus of X-over P1 (Cre/loxP) technology-based conditional gene knockouts of model mice to investigate the functional roles of genes of interest in Sertoli and Leydig cells within the testis. However, the shortcomings of these genetic tools include high costs, lengthy experimental periods, and limited accessibility for researchers. Therefore, exploring alternative gene silencing techniques is of great practical value. In this study, we employed adeno-associated virus (AAV) as a vector for gene silencing in Sertoli and Leydig cells. Our findings demonstrated that AAV serotypes 1, 8, and 9 exhibited high infection efficiency in both types of testis cells. Importantly, we discovered that all three AAV serotypes exhibited exquisite specificity in targeting Sertoli cells via tubular injection while demonstrating remarkable selectivity in targeting Leydig cells via interstitial injection. We achieved cell-specific knockouts of the steroidogenic acute regulatory (Star) and luteinizing hormone/human chorionic gonadotropin receptor (Lhcgr) genes in Leydig cells, but not in Sertoli cells, using AAV9-single guide RNA (sgRNA)-mediated gene editing in Rosa26-LSL-Cas9 mice. Knockdown of androgen receptor (Ar) gene expression in Sertoli cells of wild-type mice was achieved via tubular injection of AAV9-short hairpin RNA (shRNA)-mediated targeting. Our findings offer technical approaches for investigating gene function in Sertoli and Leydig cells through AAV9-mediated gene silencing.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143675093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sperm tRNA-derived fragments expression is potentially linked to abstinence-related improvement of sperm quality. 精子trna衍生片段的表达可能与禁欲相关的精子质量改善有关。

Asian journal of andrology Pub Date : 2025-03-18 DOI: 10.4103/aja2024123

Xi-Ren Ji, Rui-Jun Wang, Zeng-Hui Huang, Hui-Lan Wu, Xiu-Hai Huang, Hao Bo, Ge Lin, Wen-Bing Zhu, Chuan Huang

{"title":"Sperm tRNA-derived fragments expression is potentially linked to abstinence-related improvement of sperm quality.","authors":"Xi-Ren Ji, Rui-Jun Wang, Zeng-Hui Huang, Hui-Lan Wu, Xiu-Hai Huang, Hao Bo, Ge Lin, Wen-Bing Zhu, Chuan Huang","doi":"10.4103/aja2024123","DOIUrl":"https://doi.org/10.4103/aja2024123","url":null,"abstract":"Abstract: Recent studies have shown that shorter periods of ejaculatory abstinence may enhance certain sperm parameters, but the molecular mechanisms underlying these improvements are still unclear. This study explored whether reduced abstinence periods could improve semen quality, particularly for use in assisted reproductive technologies (ART). We analyzed semen samples from men with normal sperm counts (n = 101) and those with low sperm motility or concentration (n = 53) after 3-7 days of abstinence and then after 1-3 h of abstinence, obtained from the Reproductive & Genetic Hospital of CITIC-Xiangya (Changsha, China). Physiological and biochemical sperm parameters were evaluated, and the dynamics of transfer RNA (tRNA)-derived fragments (tRFs) were analyzed using deep RNA sequencing in five consecutive samples from men with normal sperm counts. Our results revealed significant improvement in sperm motility and a decrease in the DNA fragmentation index after the 1- to 3-h abstinence period. Additionally, we identified 245 differentially expressed tRFs, and the mitogen-activated protein kinase (MAPK) signaling pathway was the most enriched. Further investigations showed significant changes in tRF-Lys-TTT and its target gene mitogen-activated protein kinase kinase 2 (MAP2K2), which indicates a role of tRFs in improving sperm function. These findings provide new insights into how shorter abstinence periods influence sperm quality and suggest that tRFs may serve as biomarkers for male fertility. This research highlights the potential for optimizing ART protocols and improving reproductive outcomes through molecular approaches that target sperm function.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0