Annales de biologie clinique最新文献

{"title":"Analytical interference in CA 19-9 immunoassay generates false-positive results in a young woman with a low-grade appendiceal tumor: An educational case report and brief literature review.","authors":"Jérôme A Denis, Mathieu Boissan, Eric Rouzaud, Jean-Marc Lacorte, Marc Pocard, Laurent Genser","doi":"10.1684/abc.2024.1892","DOIUrl":"10.1684/abc.2024.1892","url":null,"abstract":"<p><p>CA 19-9 (carbohydrate antigen 19-9) is a tumor marker widely used for the follow-up of patients with pancreatic cancer and other digestive neoplasia. This case report describes a discrepancy between the results of serum CA 19-9 analyses using the Alinity analytical platform (Abbott™) and two other techniques, Kryptor Gold (ThermoFisher Scientific™) and Cobas E411 (Roche™), in the context of a young woman with appendiceal mucocele. In this context, when the serum concentration of CA 19-9 is high, it may raise concerns about potential malignancy or rupture of the mucocele that may lead to tumoral dissemination in the abdominal cavity. In the present case, we observed with Alinity a false elevation in CA 19-9 concentration at 190 kU/L (normal range < 37 kU/L) before appendix resection that continued to increase until reaching 619 kU/L six months after surgery. This situation led to unnecessary additional tests, increased hospitalization time and stress for the patient who also had to interrupt her medically assisted reproduction project. We solved this case using new measurements in CA 19-9 concentration with two other techniques, Kryptor Gold and Cobas E411, and we identified an analytical interference caused by the presence of heterophile antibodies. In all cases, abnormal result initially obtained with Alinity was found below normal range not only with the two other techniques but also with Alinity after a neutralisation step by using Heterophile Blocking Tubes (Scantibodies Laboratory™). Analytical interferences in medical tests can lead to inappropriate medical care. It is an important issue requiring a continuing training of biologists who must be aware of these problems, which are recurring concerns and are not always easy to identify in laboratories of medical biology, in particular when immunoassays are used. This case report also provides an opportunity to do a brief review of the literature and to remind some recommendations and actions to take into consideration in the presence of discrepancies between the clinic and the biology, in particular, one of them is to measure the biological analyte with a different technique. Moreover, the use of Heterophile Blocking Tubes neutralizing specifically the heterophile antibodies may be useful. In all cases, dialogue between clinicians and biologists remains essential.</p>","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"82 3","pages":"321-328"},"PeriodicalIF":0.0,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141903907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Validation of a method for measuring the antielastolytic activity of human circulating alpha1-antitrypsin].","authors":"Magali Dechomet, Farid Zerimech, Colette Chapuis-Cellier, Christine Lombard, Malika Balduyck","doi":"10.1684/abc.2024.1893","DOIUrl":"10.1684/abc.2024.1893","url":null,"abstract":"<p><p>The existence of alpha-1 antitrypsin variants with apparently unremarkable phenotypes and serum concentrations, contrasting with a clinical picture suggestive of a severe deficiency, led us to investigate whether in these cases there was a reduction or even suppression of the capacity of alpha-1 antitrypsin to inhibit elastase. To this end, in two different laboratories, we adapted and validated a method for measuring the functional activity of alpha-1 antitrypsin, based on spectrophotometric kinetic analysis of the inhibition by serum alpha-1 antitrypsin of the hydrolytic activity of porcine pancreatic elastase on a chromogenic substrate. This method has proved to be robust, reproducible and transferable and made possible to define, on the basis of an analysis of a hospital population, a functionality index with a confidence interval comprised between 0.87 and 1.2, allowing to identify subjects likely to have a functional deficiency of alpha-1 antitrypsin, whether this deficiency being of a genetic origin without any quantitative or phenotypic translation, or whether being acquired under the effect of external agents (cigarette smoke or viruses).</p>","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"82 3","pages":"308-320"},"PeriodicalIF":0.0,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141989722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[An expanding spleen].","authors":"Anastasia Milojkovic, Anne-Margaux Dejean, Marianne Tarnat, Marie-Liesse Etienne, Sarah Bugier","doi":"10.1684/abc.2024.1889","DOIUrl":"10.1684/abc.2024.1889","url":null,"abstract":"<p><p>A 24 years-old woman was admitted to hospital for an epistaxis that had not resolved. Biological results show bi-cytopenias (anemia and thrombopenia). Clinically she presents a fourth grade splenomegaly. To explore these cytopenias, a myelogram is performed.</p>","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"82 3","pages":"329-332"},"PeriodicalIF":0.0,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140900658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Intoxications with trace metal elements: A new concept of the metal profile].","authors":"Youssef Moutaouakkil, Radia Mounir, Mina Ait El Cadi, Jamel Lamsaouri, Yassir Bousliman, Rachid ElJaoudi","doi":"10.1684/abc.2024.1898","DOIUrl":"10.1684/abc.2024.1898","url":null,"abstract":"<p><p>Present in quantities ranging from hundreds of milligrams to several grams in various environmental compartments, trace metal elements (TMEs), formerly known as \"heavy metals,\" have been the subject of much discussion in recent years due to their major toxicological impact on human health. They are divided into essential elements such as iron, zinc, copper, selenium, manganese, and toxic elements such as mercury, cadmium, lead, lithium, and arsenic. Essential trace elements play a vital role in the body, and their deficiency can alter important physiological functions, while toxic elements have no physiological role and can lead to serious illnesses. However, essential elements can also cause intoxication depending on their concentrations and chemical forms. The industrialization of the modern world exposes the global population to high concentrations of trace elements that can be toxic in the short or long term. These TMEs enter the human body through various pathways (inhalation, ingestion, or dermal contact). The clinical manifestations of TME intoxications are highly varied and nonspecific, making their diagnosis and management difficult. Inductively coupled plasma mass spectrometry (ICP-MS) allows the detection of TME intoxications. Indeed, it enables the simultaneous quantification of around thirty trace metal elements in biological media. Thus, it has led to the establishment of a new approach: the metal profile, which has many applications in clinical biology and clinical, forensic, occupational, or environmental toxicology.</p>","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"82 3","pages":"254-265"},"PeriodicalIF":0.0,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141989720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Professor Philippe Gillery honoured at WorldLab Dubai (UAE) with the IFCC Howard Morris 2024 Prize].","authors":"Bernard Gouget, Katell Peoc'h","doi":"10.1684/abc.2024.1894","DOIUrl":"10.1684/abc.2024.1894","url":null,"abstract":"","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"82 3","pages":"249-251"},"PeriodicalIF":0.0,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141989721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unexpected diagnosis of Enterobius vermicularis infection by urine sediment analysis on sediMAX conTRUST PRO.","authors":"Sophie Herens, Giang Ho, Pierre-Alexandre Olivier, Terry Laurent","doi":"10.1684/abc.2024.1890","DOIUrl":"10.1684/abc.2024.1890","url":null,"abstract":"","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"82 2","pages":"239-240"},"PeriodicalIF":0.0,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140900654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Factor XI deficiency: actuality and review of the literature].","authors":"Marine Demoy, Julien Labrousse, François Grand, Stéphanie Moyrand, Marie Tuffigo, Solene Lamarche, Laurent Macchi","doi":"10.1684/abc.2024.1884","DOIUrl":"10.1684/abc.2024.1884","url":null,"abstract":"<p><p>Although two clusters have been identified in France, constitutional factor XI deficiency is a rare disorder. Acquired factor XI deficiency is extremely rare. The management of factor XI deficiency is not staightforward because of the unpredictable bleeding tendency that does not clearly relate to the factor XI level. Other haemostastis parameters have to be taken into account to evaluate the bleeding tendency. We report the cases of a congenital factor XI deficiency, an acquired factor XI deficiency and a von Willebrand disease associated to a factor XI deficiency. On the other hand, some interferences can lead to underestimation of factor XI and we report the case of an interference by lupus anticoagulant. The objective of this review is to better understand how to manage a reduced factor XI level.</p>","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"82 2","pages":"225-236"},"PeriodicalIF":0.0,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140871694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Six Sigma driven QC management in hepatitis C serology].","authors":"Mohamed Mokhtar Khelil","doi":"10.1684/abc.2024.1885","DOIUrl":"10.1684/abc.2024.1885","url":null,"abstract":"<p><p>The Westgard quality control (QC) rules are often applied in infectious diseases serology to validate the quality of results, but this requires a reasonable tradeoff between maximum sensitivity to errors and minimum false rejections. This article, in addition to illustrate the six sigma methodology in the QC management of the (anti-HCV Architect®) test, it discusses the main influencing factors on sigma value. Data from low positive and in-kit control materials spreading over 6 months and using four reagent kits, were used to calculate the precision of the test. The difference between the control material reactivity and the cut-off defined the error budget. Sigma values were > 6, which indicates that the method produces four erroneous results per million tests. The application of the six sigma concept made it possible to argue the choice of the new QC strategy (use of 13S rule with one positive control) and to relax the existing QC rules. This work provides a framework for infectious diseases serology laboratories to evaluate tests performances against a quality requirement and design an optimal QC strategy.</p>","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"82 2","pages":"215-224"},"PeriodicalIF":0.0,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140856868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Cybersecurity and cyber-resilience: a summary of the SFBC 2023 session at the 2023 Biology Innovation Days].","authors":"Laurence Mouly, Nasser Amani, Camille Gobeaux, Gilles Defrance, Maxime Vandershooten, Jérôme Vétillard, Katell Peoc'h","doi":"10.1684/abc.2024.1881","DOIUrl":"10.1684/abc.2024.1881","url":null,"abstract":"","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"82 2","pages":"241-244"},"PeriodicalIF":0.0,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140857645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of small dense LDL-C/large buoyant LDL-C ratio as an independent risk factor in patients with type 2 diabetes mellitus and metabolic syndrome","authors":"Cui Wang, Yaoze Li, Pei Peng, Hao Lin, Yulin Zhu, Xiangdong Xu, PingPing Wu, Yali Zhang, Ting Xu, Jian Ren, Haibo Xu, Guoyu Dong, Yue Xu, Huihui Jiang, Jiangman Zhao, Yufeng Zhai, Guotian Zai","doi":"10.1684/abc.2024.1883","DOIUrl":"10.1684/abc.2024.1883","url":null,"abstract":"<p><p>Dyslipidemia plays a key role in metabolic syndrome (MS), intricately linked to type 2 diabetes mellitus (T2DM). This study aimed to investigate the differences in low-density lipoprotein cholesterol (LDL-C) subfraction levels between T2DM and T2DM with MS, and identify the risk factors associated with the disease. A total of 246 individuals diagnosed with T2DM, including 144 T2DM patients with MS, and 147 healthy subjects were recruited. All participants underwent a comprehensive clinical evaluation. Lipoprotein subfraction analysis was performed using the Lipoprint LDL system. Multivariate logistic regression analysis revealed that several lipid markers, including triglyceride (TG), LDL-C, large buoyant LDL-C (lbLDL-C), small dense LDL-C (sdLDL-C), LDLC2-5, and sdLDL-C/lbLDL-C ratio, were identified as independent risk factors for T2DM. Additionally, TG, sdLDL-C, LDLC-4, LDLC-5, and sdLDL-C/lbLDL-C ratio were found to be independent risk factors for T2DM with MS. Furthermore, the results of the receiver operating characteristic (ROC) curves demonstrated that sdLDL-C, LDLC-4, LDLC-3, and sdLDL-C/lbLDL-C ratio exhibited excellent predictive performance for the risk of T2DM (AUC > 0.9). The sdLDL-C/lbLDL-C ratio emerges as a shared independent risk factor for T2DM and MS complications. Furthermore, sdLDL-C/lbLDL-C ratio, along with LDL-4 and LDL-3, exhibits noteworthy predictive capabilities for T2DM.</p>","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"82 2","pages":"174-186"},"PeriodicalIF":0.0,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141238941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}