Na Zhang, Yue Xu, Hao Liang, Qingsong Wang, Yu An, Haichao Gao, Jiangman Zhao, Hong Wang
{"title":"Diagnostic value of small dense low-density lipoprotein and trace elements in coronary artery disease.","authors":"Na Zhang, Yue Xu, Hao Liang, Qingsong Wang, Yu An, Haichao Gao, Jiangman Zhao, Hong Wang","doi":"10.1684/abc.2025.1960","DOIUrl":"https://doi.org/10.1684/abc.2025.1960","url":null,"abstract":"<p><p>Coronary artery disease (CAD) is a worldwide leading cause of death. Considering that 20%-40% of patients with CAD have a long asymptomatic period of atherosclerosis, it has become urgent to explore the feasibility of diagnosing CAD at an early stage. This is an observational, case-control study, a total of 489 consecutive CAD patients and 75 non-CAD controls were recruited. The levels of low-density lipoprotein subfractions (LDLC1-7) in serum were measured by the Quantimetrix Lipoprint LDL system. The levels of 18 trace elements (vanadium, chromium, manganese, cobalt, nickel, copper, zinc, gallium, arsenic, selenium, strontium, cadmium, tin, antimony, barium, mercury, thallium, and lead) were tested using inductively coupled plasma mass spectrometry. Six machine learning algorithms (Logistic Regression, K Neighbors, GaussianNB, Random Forest, Decision Tree and XGBoost) were used to construct CAD diagnostic models. The levels of LDLC-3, LDLC-4, LDLC-5, and lead were significantly higher in CAD patients, while the levels of LDLC-1, chromium, manganese, cobalt, and strontium were lower (p < 0.05 for all). Univariate logistic regression analysis indicates that LDLC-3, LDLC-4, and lead were the risk factors for CAD development (odds ratio >1 and p < 0.05 for all), while LDLC-1, chromium, manganese, cobalt, and strontium were the protective factors for CAD (odds ratio < 1 and p < 0.05 for all). XGBoost had the best overall diagnostic performance among the six algorithms. There are significant differences in the levels of several LDL subfractions and trace elements between non-CAD controls and CAD patients. These biomarkers may help the diagnostic of CAD while applying machine learning algorithms.</p>","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"83 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143733109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Overview of practices among French clinical pathologists specialized in haemostasis].","authors":"Andréas Perrier-Cornet, Nicolas Gendron, Céline Desconclois, Virginie Planche, Caroline Vayne, Fabienne Nedelec Gac, Cécile Lavenu-Bombled, Dominique Lasne, Claire Flaujac, Myriam Hormi","doi":"10.1684/abc.2025.1959","DOIUrl":"https://doi.org/10.1684/abc.2025.1959","url":null,"abstract":"<p><p>Medical biology is a medical specialty shared by medical doctor (MD) and pharmacists (PharmD), and Hemostasis is one of its disciplines. Since 2007, physicians have lost interest in medical biology. In haemostasis, the regulatory heterogeneity of status between medical biologists/MDs and medical biologists/PharmDs in a strained health system raises two major issues: (i) with demographic change, optimal patient care may no longer be guaranteed, (ii) medical biologists/PharmDs are sometimes forced to go beyond the scope of their regulatory field of practice. We conducted a survey in 2022 to examine hemostasis advice and consultation practices: 152 professionals responded, including 99 pharmacist-biologists (65.1%), 42 physician-biologists (27.6%) and 11 clinicians (7.2%). Of the practitioners questioned, 91.9% gave diagnostic advice and 75.0% therapeutic advice. 41% of respondents carried out haemostasis consultations, including 24.2% of pharmacists. Our survey reveals a little-known role for medical biologists specialized in haemostasis, particularly pharmacists, and calls for a global reflection on a possible regulated and supervised evolution of their field of practice, to enable them to pursue their mission in complete safety.</p>","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"83 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143722977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sarah Diabate, Céline Bouchet-Seraphin, Sandrine Vuillaumier-Barrot, Guillaume Lefevre, Katell Peoc'h
{"title":"[Increased troponin T in a patient with myositis].","authors":"Sarah Diabate, Céline Bouchet-Seraphin, Sandrine Vuillaumier-Barrot, Guillaume Lefevre, Katell Peoc'h","doi":"10.1684/abc.2025.1954","DOIUrl":"10.1684/abc.2025.1954","url":null,"abstract":"<p><p>A 41-year-old woman presented to the emergency room for long-term dysphagia, with a loss of eight kilos in two months. Myositis or inflammatory myopathies were suspected. A marked increase in troponin T concentration was observed.</p>","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"83 1","pages":"109-112"},"PeriodicalIF":0.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bone marrow involvement by breast carcinoma mimicking blast cells: the utility of CD326.","authors":"Radu Chiriac, Luc-Marie Gerland, Lucile Baseggio","doi":"10.1684/abc.2025.1952","DOIUrl":"10.1684/abc.2025.1952","url":null,"abstract":"","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"83 1","pages":"105-106"},"PeriodicalIF":0.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Olivier Meunier, Sandrine Burger, Cécile Garniche, Marie Delfino, Julien Exinger
{"title":"[Incidence of healthcare-associated infections in 2023 at the Haguenau Hospital Centre].","authors":"Olivier Meunier, Sandrine Burger, Cécile Garniche, Marie Delfino, Julien Exinger","doi":"10.1684/abc.2025.1943","DOIUrl":"10.1684/abc.2025.1943","url":null,"abstract":"<p><p>Nosocomial infections (NI) are a major concern of healthcare professionals. To propose targeted awareness-raising campaigns, we put in place NI monitoring of laboratory data. The positive bacteriological results after 48 hours hospitalisation are subject of a report via the Infection Control Team, to the physician corresponding in the department, for the clinical validation of diagnostic of NI. At hospital level and specialty activity, we measured NI incidence rates for 1 000 hospital days and for 100 admissions every month since October 1, 2016. Results were then declined by specialties and by infected anatomical site and investigation allowed to describe the microbial flora responsible for these infections. The request began in october 2016. Average results were stable over the year despite monthly fluctuations. For 2023, incidence rates are IAS 2,8 for 1 000 JH and IAS 1,2 for 100 admissions. The detail of the results is transmitted to the physicians and is the subject of internal communications. They will be allowed to follow up more closely with the NI epidemiology and propose targeted corrective measures as appropriate.</p>","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"83 1","pages":"9-19"},"PeriodicalIF":0.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Assessment of serum free light chains in patients with systemic lupus erythematosus].","authors":"Ines Allam, Aldjia Lamri, Sihem Oualakrouz, Messaoud Saidani, Soraya Ayoub, Brahim Belaid, Reda Djidjik","doi":"10.1684/abc.2025.1948","DOIUrl":"10.1684/abc.2025.1948","url":null,"abstract":"<p><p>Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by loss of B cell tolerance to self antigens resulting in the production of a wide variety of auto antibodies. The objective of this study was the assessment of serum free light chains (FLC) assay in patients with SLE. In this study, 156 patients (142 women and 14 men, mean age 35.6 years) and 60 healthy subjects (55 women and 5 men, mean age 36.4 years) were included. The kappa (κ) and lambda (λ) FLC were measured by turbidimetry method. The analysis of our results showed that the FLC rates κ and λ were significantly higher in patients compared to controls (44.52 vs. 8,55 mg/L, P = 0.0001 for FLC κ and 33.43 vs. 12,99 mg/L, P = 0.0001 for FLC λ). In addition, significant associations were noted between the increase in FLC κ, λ and disease activity (P = 0.006 and P = 0.008 respectively), as well as with the presence of lupus nephritis (P = 0.044 and p = 0.045). The increase of the serum FLC during SLE could be a useful marker for assessing the disease activity and the risk of lupus nephritis.</p>","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"83 1","pages":"113-116"},"PeriodicalIF":0.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143443010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Multi-omics analysis reveals the molecular mechanisms and therapeutic targets in high altitude polycythemia.","authors":"Yanyan Li, Chengwen Wang, Haiyan Ma, Minghui Wang","doi":"10.1684/abc.2025.1945","DOIUrl":"10.1684/abc.2025.1945","url":null,"abstract":"<p><p>Polycythemia, characterized by an abnormal increase in red blood cells, can manifest as primary polycythemia (PV) or secondary polycythemia, often due to conditions like obstructive sleep apnea (OSA) and high-altitude polycythemia (HAP). Understanding the molecular mechanisms underlying these conditions is crucial for developing targeted therapies. Transcriptome analysis was performed to identify differentially expressed genes (DEGs), significant modules, and immune cell infiltration. We identified 370 DEGs and four significant gene co-expression modules closely related to HAP. Modules were associated with neutrophil migration, glycoprotein metabolism, UDP-N-acetylglucosamine synthesis, and red pulp cells. These modules showed the same directions of change in OSA but were reversed when treated with continuous positive airway pressure therapy. Comparison with GWAS showed that overlapping genes were related to oxygen transport in the DEGs. PRDM1 and NCOA1, NFE2 were identified as key transcription factors. Noca1 knockout mice showed elevated levels of red blood cell count (RBC), hemoglobin (HGB), and hematocrit (HCT). Lomustine and hydralazine emerged as potential candidate drugs for treating polycythemia. Abdominal breathing training for three months improved symptoms and reduced RBC, HGB, and HCT in 33 HAP patients. These findings elucidate HAP's molecular mechanisms and suggest new therapeutic directions.</p>","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"83 1","pages":"20-34"},"PeriodicalIF":0.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Multicenter analytical performance evaluation of the ST Genesia thrombin generation system.","authors":"Laurie Talon, Joffrey Feriel, Thomas Sinegre, Aurélien Lebreton, Mélanie Lecomte, Claire Hemar","doi":"10.1684/abc.2025.1942","DOIUrl":"10.1684/abc.2025.1942","url":null,"abstract":"<p><p>Available data on the potential use of thrombin generation (TG) assays in clinical practice is promising but larger studies involving several centers are needed to confirm this added-value for clinical purposes. The objective of this evaluation was to assess the analytical performances of the ST Genesia using STG-ThromboScreen, STG-BleedScreen, and STG-DrugScreen reagents across three centers to support its use in multicenter studies. Repeatability and reproducibility have been evaluated using commercial plasmas and quality control (QC) samples. Accuracy was assessed by calculating QC biases from the manufacturer's assigned values. Frozen plasma samples from healthy donors and patients having thrombophilia, hemorrhagic disorders, or taking anticoagulants allowed the comparison of TG parameters between the analyzers from two French centers. Repeatability and reproducibility CVs were respectively below 5% and 10% whatever the reagent and the sample used. The later fell under 6% after normalization. Mean biases between observed and assigned QC values provided by the manufacturer were less than 5% for most TG parameters. We found a good agreement for all TG parameters between the two evaluating centers. Relative bias was below 5% for all combination of parameters and reagents except for Peak height (+14.2%), ETP (+7.3%), and ETP inhibition (-12.0%) using STG-ThromboScreen. We found satisfactory repeatability, reproducibility, accuracy, and inter-laboratory variability for TG parameters using the three available reagents across three centers, supporting the use of ST Genesia in multicenter clinical trials.</p>","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"83 1","pages":"35-48"},"PeriodicalIF":0.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143451222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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