Annales de biologie clinique最新文献

BIA-ALCL diagnosis: the relevance of cytology and flow cytometry in periprosthetic fluid analysis. BIA-ALCL诊断：细胞学和流式细胞术在假体周围液分析中的相关性。

Annales de biologie clinique Pub Date : 2025-05-30 DOI: 10.1684/abc.2025.1976

Radu Chiriac, Marie Donzel, Lucile Baseggio

{"title":"BIA-ALCL diagnosis: the relevance of cytology and flow cytometry in periprosthetic fluid analysis.","authors":"Radu Chiriac, Marie Donzel, Lucile Baseggio","doi":"10.1684/abc.2025.1976","DOIUrl":"https://doi.org/10.1684/abc.2025.1976","url":null,"abstract":"Breast implant-associated anaplastic large cell lymphoma (BIA-ALCL) is a rare T-cell non-Hodgkin lymphoma, representing less than 1% of breast neoplasms. Despite its low incidence, the increasing number of women with breast implants necessitates vigilance among clinicians and pathologists. BIA-ALCL presents in situ and invasive forms, with varying prognoses. The National Comprehensive Cancer Network guidelines recommend cytology, immunohistochemistry, and flow cytometry (FCM) for diagnosis. This retrospective study analyzed 16 periprosthetic fluid (PF) samples from suspected lymphoma cases between January 2018 and January 2024. Cytological and immunological analyses were performed using May Grünwald-Giemsa staining, and FCM with BD FACSCanto II and BD LYRIC cytometers. A 10 or 12-colour FCM panel including pan-T markers and CD30 was used. Of the 16 samples, 2 cases were diagnosed with BIA-ALCL. BIA-ALCL cases showed atypical CD4+ T-cells with large size and loss of CD3 and CD7. In contrast, the 14 reactive cases did not exhibit atypical cells. Key challenges included sample volume limitations, cell dilution, and distinguishing neoplastic cells from reactive ones, particularly in cases with low cell counts. FCM, combined with an extensive panel of pan-T markers and CD30, effectively differentiates anaplastic BIA-ALCL cells from reactive seroma. However, it should complement, not replace, traditional diagnostic methods. Recognizing pitfalls and correlating FCM findings with clinical and morphological data enhances diagnostic accuracy. FCM is a valuable tool for diagnosing BIA-ALCL but should be used alongside other methods. Accurate diagnosis depends on understanding sample-specific challenges and integrating FCM results with clinical context.","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"83 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Signet-ring plasma cell lymphoplasmacytic lymphoma: a rare twist. 印戒浆细胞淋巴浆细胞淋巴瘤：罕见的扭曲。

Annales de biologie clinique Pub Date : 2025-05-30 DOI: 10.1684/abc.2025.1977

Radu Chiriac, Valentin Pourchet, Lucile Baseggio

引用次数: 0

Unseen dangers: when myeloma and carcinoma strike together. 看不见的危险：当骨髓瘤和癌症同时发作。

Annales de biologie clinique Pub Date : 2025-05-30 DOI: 10.1684/abc.2025.1978

Radu Chiriac, Lucile Baseggio

引用次数: 0

Vérification des performances de la méthode COBAS® AmpliPrep/COBAS® TaqMan HIV-1 test, v 2.0 pour la quantification de la charge virale plasmatique du VIH-1. 验证COBAS®AmpliPrep/COBAS®TaqMan HIV-1测试v2.0的性能，用于HIV-1血浆病毒载量的量化。

Annales de biologie clinique Pub Date : 2025-05-30 DOI: 10.1684/abc.2025.1972

Ella Christelle Brou, Aboubakar Touré, Fidèle Sounan Touré, Eugénie Yaudé, Inza Dosso, Marcellin Konan, Durand Sery, Christiane Kiki-Barro

{"title":"Vérification des performances de la méthode COBAS® AmpliPrep/COBAS® TaqMan HIV-1 test, v 2.0 pour la quantification de la charge virale plasmatique du VIH-1.","authors":"Ella Christelle Brou, Aboubakar Touré, Fidèle Sounan Touré, Eugénie Yaudé, Inza Dosso, Marcellin Konan, Durand Sery, Christiane Kiki-Barro","doi":"10.1684/abc.2025.1972","DOIUrl":"https://doi.org/10.1684/abc.2025.1972","url":null,"abstract":"The laboratory of the National Institute of Public health makes HIV-1 plasma RNA quantification. That test is required to follow up HIV patient on ART (Antiretroviral therapy). The laboratory was accredited by the West African Accreditation System (SOAC) in 2022 for HIV real time PCR. Here we report the experience of the laboratory for verifiying performance of the COBAS® AmpliPrep/COBAS® TaqMan (CAP/CTM) HIV-1 test, v 2.0 kit for measuring viral load by real time PCR. According with the technical guides of the French Accreditation Committee (COFRAC) and the recommendations of the French Society of Clinical Biology (SFBC), different steps were followed. These are the risk assessment, the study of reliability, intermediate precision, accuracy and precision of the method, estimation of measurement uncertainty and comparison of methods. Coefficient of variation for repeatability, reproducibility and trueness were within target of 5 %. The results from the CAP/CTM were reproductible and identical to those of the COBAS 6800. The COBAS® AmpliPrep/COBAS® TaqMan HIV-1 Test, v 2.0 method is suitable for routine laboratory use and has analytical performance for its intended utility, which mean the assessment of disease progression and monitoring of antiretroviral therapy for HIV-1 patients.","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"83 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Studying daratumumab's interference in serum protein electrophoresis: two case reports]. 【研究达拉单抗对血清蛋白电泳的干扰：两例报告】。

Annales de biologie clinique Pub Date : 2025-05-22 DOI: 10.1684/abc.2025.1963

Alexandre Leonard, Benjamin Bonnet, Carolyne Croizier, Bertrand Evrard, Vincent Sapin, Anne Fogli, Damien Bouvier

引用次数: 0

Evaluation of the prognostic value of coagulation function and cerebral hemodynamic changes in acute ischemic stroke patients with rheumatoid arthritis. 急性缺血性脑卒中合并类风湿患者凝血功能及脑血流动力学变化的预后价值评价。

Annales de biologie clinique Pub Date : 2025-05-22 DOI: 10.1684/abc.2025.1965

Ying Yang, Linxin Zhang, Xing Zhao, Juan Li, Qingping Chen, Yaya Gao, Jun Yan

{"title":"Evaluation of the prognostic value of coagulation function and cerebral hemodynamic changes in acute ischemic stroke patients with rheumatoid arthritis.","authors":"Ying Yang, Linxin Zhang, Xing Zhao, Juan Li, Qingping Chen, Yaya Gao, Jun Yan","doi":"10.1684/abc.2025.1965","DOIUrl":"10.1684/abc.2025.1965","url":null,"abstract":"To explore the relationship between early coagulation changes, cerebral hemodynamics, and neurological prognosis in acute ischemic stroke (AIS) patients with rheumatoid arthritis (RA). Seventy-two AIS patients with RA were included. Coagulation markers (PT, APTT, FIB) and transcranial Doppler ultrasound (TCD) assessments of middle cerebral artery (MCA) mean velocity (Vm) and pulsatility index (PI) were measured at baseline and after 14 days of treatment. Prognosis was evaluated using the modified Rankin Scale (mRS) at 3 months, dividing patients into good (mRS ≤ 2) and poor prognosis (mRS > 3) groups. Multivariate logistic regression and ROC analysis assessed the predictive value of coagulation and hemodynamic changes for adverse outcomes. After 14 days, APTT and MCA (PI) increased, while FIB decreased in both groups (P < 0.05). The good prognosis group had higher MCA Vm and lower FIB at baseline (P < 0.05). Independent risk factors for poor prognosis included diabetes, NIHSS score ≥ 8, FIB, and MCA (Vm, PI) (P < 0.05). ROC analysis showed combined FIB, Vm, and PI had better predictive value for poor outcomes. Early hypercoagulable state and suboptimal cerebral hemodynamics are associated with poor neurological outcomes in AIS with RA.","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"83 2","pages":"185-94"},"PeriodicalIF":0.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144060811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Usefulness of the thrombin generation test in hypercoagulability states]. [凝血酶生成试验在高凝状态下的有效性]。

Annales de biologie clinique Pub Date : 2025-05-22 DOI: 10.1684/abc.2025.1955

Paul Billoir, Sébastien Miranda, Hervé Lévesque, Ygal Benhamou, Véronique Le Cam Duchez

{"title":"[Usefulness of the thrombin generation test in hypercoagulability states].","authors":"Paul Billoir, Sébastien Miranda, Hervé Lévesque, Ygal Benhamou, Véronique Le Cam Duchez","doi":"10.1684/abc.2025.1955","DOIUrl":"10.1684/abc.2025.1955","url":null,"abstract":"Thrombin is one of the key components of the hemostatic system. The thrombin generation assay (TGA) assesses the activation, propagation and inhibition of coagulation. This global coagulation test is of interest for assessing the risk of bleeding and thrombosis. Numerous studies have been performed in the \"thrombophilia\". We performed a systematic review of studies on thrombinography in thrombophilia. The included studies, published in English, were original investigations using TGT during venous thromboembolic disease, arterial occlusive pathology, pregnancy, anti-phospholipid syndrome, constitutional thrombophilias, or oncology. Eighty studies were selected between January 2003 and June 2022. Most had fewer than 100 patients. The only two studies that included more than 1000 patients showed an increased risk of thrombotic recurrence or increased mortality from cardiovascular disease associated with increased thrombin generation. All \"thrombophilia\" have been studied, but lack of standardization between conditions has limited the development of clinical research with this assessment technique. Harmonization of practices may provide more relevant systems for predicting the occurrence of arterial or venous thrombotic events.","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"83 2","pages":"121-46"},"PeriodicalIF":0.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143506705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Botryoid white-cell nuclei induced by DRESS syndrome. DRESS综合征诱导的芽孢样白细胞核。

Annales de biologie clinique Pub Date : 2025-05-22 DOI: 10.1684/abc.2025.1961

Radu Chiriac, Valentin Pourchet

引用次数: 0

[O negative blood for everyone? No, transfusion and obstetrical management of a pregnant woman with an anti-public H1I1 antibody]. 每个人都是O型阴性血？不，有抗hh1n1抗体孕妇的输血和产科管理]。

Annales de biologie clinique Pub Date : 2025-05-22 DOI: 10.1684/abc.2025.1962

Maud Gilson, Anaïs Devey

引用次数: 0

[Diagnosis of significant hepatic fibrosis in patients with chronic hepatitis B: another simple, effective and inexpensive method]. 【诊断慢性乙型肝炎患者显著肝纤维化：另一种简单、有效、廉价的方法】。

Annales de biologie clinique Pub Date : 2025-05-22 DOI: 10.1684/abc.2025.1964

Boni Guy-Martial Kouame, Bi Foua Jonas Vanie, Amena Guy Germaine Niamke, Kouzahon Colombe Jeannine Lohore, Aké Chibrou Bénédicte Yapo-Kee, Youzan Ferdinand Djohan

引用次数: 0