Annales de biologie clinique最新文献

[Building today the sustainable medical biology laboratory of tomorrow]. [建设明天可持续发展的医学生物学实验室]。

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Annales de biologie clinique Pub Date : 2025-10-10 DOI: 10.1684/abc.2025.1995

Julien Brunier, Marine Cargou, Nicolas Gendron, Camille Kolenda, Bérénice Schell, Julien Obiols, Anne Rullier, Caroline Sarre-Pradon

引用次数: 0

[Importance of bilirubinemia in the daily management of neonatal jaundice in a university hospital group: problems and solutions]. 胆红素血症在某大学附属医院新生儿黄疸日常管理中的重要性：问题及对策

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Annales de biologie clinique Pub Date : 2025-10-09 DOI: 10.1684/abc.2025.1994

Agnès Mailloux, Rana Alkouri, Dominique Bonnefont-Rousselot, Sophie Bailleul, Laure Calas, Erwan Guyot, Marie Clotilde Haguet, Nathalie Mario, Michel Vaubourdolle

{"title":"[Importance of bilirubinemia in the daily management of neonatal jaundice in a university hospital group: problems and solutions].","authors":"Agnès Mailloux, Rana Alkouri, Dominique Bonnefont-Rousselot, Sophie Bailleul, Laure Calas, Erwan Guyot, Marie Clotilde Haguet, Nathalie Mario, Michel Vaubourdolle","doi":"10.1684/abc.2025.1994","DOIUrl":"https://doi.org/10.1684/abc.2025.1994","url":null,"abstract":"Insufficient standardization and comparability of total bilirubin assays in neonates pose a problem for the interpretation of results, in application of national and international recommendations for the management of neonatal ejaundice. We present the results of a study carried out in the AP-HP Sorbonne Université group. Intra-laboratory and inter-technique variations were assessed on switchable control samples prepared by the Centre National de Référence en Hémobiologie Périnatale. Results were compared with those obtained using the connected validation technique. The results of the accuracy studies showed performance in line with clinical needs in neonatology for all the methods tested. Accuracy studies were used to check whether the clinical interpretation of results was relevant to consensus criteria. The results confirmed the trends observed in a national multicenter SFBC (Société Française de Biologie Clinique) - CNBH (Collège National de Biochimie des Hôpitaux) - CNRHP (Centre National de Référence en Hémobiologie Périnatale) study and in the CNRHP recommendations on the subject. In conclusion, a good knowledge by the medical biologist of the recommended criteria for interpretation and therapeutic decisions, of the limits and performance of the techniques used, and a close clinicobiological partnership are essential for optimum efficiency in the management of neonatal jaundice.","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"83 5","pages":"0"},"PeriodicalIF":0.4,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145254047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of NaCl 9‰ as a cheap diluent for cortisol assay in lateralized samples from adrenal veins catheterisation. 用氯化钠9‰作为廉价稀释剂对肾上腺静脉置管的侧化样品进行皮质醇测定。

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Annales de biologie clinique Pub Date : 2025-08-29 DOI: 10.1684/abc.2025.1993

Aurélie Sieuw, Julie Brossaud, Jean-Benoît Corcuff, Cindy Lauro, Agnès Georges

{"title":"Use of NaCl 9‰ as a cheap diluent for cortisol assay in lateralized samples from adrenal veins catheterisation.","authors":"Aurélie Sieuw, Julie Brossaud, Jean-Benoît Corcuff, Cindy Lauro, Agnès Georges","doi":"10.1684/abc.2025.1993","DOIUrl":"https://doi.org/10.1684/abc.2025.1993","url":null,"abstract":"Among the tools necessary for the diagnosis of primary hyperaldosteronism, catheterization of the adrenal veins represents the major examination to determine the presence of lateralized aldosterone secretion. To ensure that the catheter is well positioned at the level of the adrenal veins, a cortisol dosage is carried out in parallel with the aldosterone dosage. At the Hormonology and Tumor Markers laboratory of the Bordeaux University Hospital, this cortisol assay is carried out on an Abbott Architect i2000, making it possible to extend the calibration range up to > 3,300 nmol/L. Beyond that, the supplier recommends carrying out a manual dilution using calibrator A in which the cortisol concentration is equal to 0 nmol/L. The downside is that this calibrator cannot be supplied alone. It is only available in a common box with the 5 other calibrators necessary to carry out the cortisol calibration range. To overcome this expensive strategy, we studied the use of another diluent: 0.9% NaCl. Samples from 11 CVS were diluted with 0.9% NaCl and a comparison of cortisol results was performed (n = 128). Passing-Bablok regression of cortisol concentrations did not show significant deviation from linearity. The interpretation of CVS selectivity was not impacted by the change of diluent nor the interpretation of secretion lateralization. This change of diluent therefore did not modify the appropriate medical decision: medicinal treatment in the case of the identification of a bilateral secretion or proposal for surgical intervention in the case of the identification of a lateralized secretion.","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"83 5","pages":"0"},"PeriodicalIF":0.4,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144982222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

When code crashes the lab: Operational resilience in clinical laboratories amid a cyberattack - A case study from a university hospital. 当代码使实验室崩溃时：网络攻击中临床实验室的操作弹性-来自大学医院的案例研究。

IF 0.4

Annales de biologie clinique Pub Date : 2025-08-26 DOI: 10.1684/abc.2025.1988

Christian Al Haddad, Tigresse Boutros, Peter Finianos, Myrna Germanos

{"title":"When code crashes the lab: Operational resilience in clinical laboratories amid a cyberattack - A case study from a university hospital.","authors":"Christian Al Haddad, Tigresse Boutros, Peter Finianos, Myrna Germanos","doi":"10.1684/abc.2025.1988","DOIUrl":"10.1684/abc.2025.1988","url":null,"abstract":"Cyberattacks on healthcare organizations have escalated globally, resulting in serious risks to patient care and safety. Clinical laboratories, relying heavily on data-intensive systems, are particularly vulnerable to disruptions when hospital information systems are compromised. We conducted a single-center retrospective case study of a major cyberattack affecting the laboratory services at Notre Dame des Secours-UH in Lebanon. Operational data, incident reports, and recovery timelines were reviewed to characterize the attack's impact on laboratory operations and the resilience measures implemented. The cyberattack led to an immediate shutdown of laboratory information systems and automation, necessitating a shift to paper-based and manual processes. Key emergency protocols were activated within hours, including manual test ordering, handwritten result transcription with double verification, and specialized staff-controlled release of blood products. Critical services were maintained, but routine testing and volumes dropped sharply in the first week. A stepwise recovery ensued: by day 3 a limited laboratory information systems functionality was restored on a local network, by day 10 most laboratory services resumed albeit with workflow adjustments, and normal operations were largely re-established within two months. Our case points out to the operational resilience of a clinical laboratory during a prolonged cyber crisis. Effective crisis management, including timely incident response planning, staff adaptability, and emergency procedures, improved patient care in such a dreaded organizational situation and allowed for the return to normal workflow of the clinical laboratory.","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"83 4","pages":"414-424"},"PeriodicalIF":0.4,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144796397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blastoid-appearing lymphocytes in a case of leukemic marginal zone lymphoma [白血病期边缘区域淋巴瘤中母细胞样淋巴细胞]。

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Annales de biologie clinique Pub Date : 2025-08-26 DOI: 10.1684/abc.2025.1990

Radu Chiriac, Estelle Bourbon, Lucile Baseggio

引用次数: 0

[Monocytes and cancer: fundamental insights and therapeutic perspectives]. [单核细胞和癌症：基本见解和治疗观点]。

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Annales de biologie clinique Pub Date : 2025-08-26 DOI: 10.1684/abc.2025.1984

Bouchra M'raouni, Ikram Souli, Nadia Lakhouaja, Saad Lamjadli, Abdelmouine Salami, Fatima Ezzohra Eddehbi, Hamza Oualhadj, Raja Hazime, Brahim Admou

{"title":"[Monocytes and cancer: fundamental insights and therapeutic perspectives].","authors":"Bouchra M'raouni, Ikram Souli, Nadia Lakhouaja, Saad Lamjadli, Abdelmouine Salami, Fatima Ezzohra Eddehbi, Hamza Oualhadj, Raja Hazime, Brahim Admou","doi":"10.1684/abc.2025.1984","DOIUrl":"10.1684/abc.2025.1984","url":null,"abstract":"Monocytes, circulating mononuclear phagocytes, play a fundamental role in innate immunity and the maintenance of tissue homeostasis. Using advanced technologies like flow cytometry, the characterization of monocytes has evolved from a simplistic view of a homogeneous population to a more complex understanding of a heterogeneous system comprising three main subtypes: classical monocytes (CD14++CD16-), intermediate monocytes (CD14++CD16+), and non-classical monocytes (CD14+CD16++). The identification of these subpopulations has enabled precise characterization of their functional profiles, enhancing the understanding of their roles in various pathological contexts, particularly in oncology. While anti-tumoral functions of monocytes have been clearly established in certain categories of cancers through tumor antigen presentation, induction of cytotoxic responses, and inhibition of metastatic progression, their role in promoting the development and progression of other cancers has also been highlighted during recent years. The utilization of monocytes in cancer immunotherapy presents promising opportunities, particularly by reprogramming their activity to enhance anti-tumoral responses or suppress their pro-tumoral functions. This review provides a comprehensive analysis of recent advances in the phenotypic and functional diversity of monocytes and their role in tumor progression, while highlighting emerging therapeutic strategies targeting these cells to optimize cancer treatment.","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"83 4","pages":"357-371"},"PeriodicalIF":0.4,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144762645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Example of a genetic condition caused by an imprinting disorder: Angelman syndrome]. [由印记紊乱引起的遗传状况的例子：天使综合症]。

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Annales de biologie clinique Pub Date : 2025-08-26 DOI: 10.1684/abc.2025.1982

Charlene Coquisart, Sana Skouri, Geoffroy Delplancq, Myrtille Spentchian, Benjamin Maneglier, Mihelaiti Guberto, Sophie Brisset

{"title":"[Example of a genetic condition caused by an imprinting disorder: Angelman syndrome].","authors":"Charlene Coquisart, Sana Skouri, Geoffroy Delplancq, Myrtille Spentchian, Benjamin Maneglier, Mihelaiti Guberto, Sophie Brisset","doi":"10.1684/abc.2025.1982","DOIUrl":"10.1684/abc.2025.1982","url":null,"abstract":"An 18-months old boy was seen in a clinical genetics consultation with both his parents for a global developmental delay, hypotonia, post-natal microcephaly, as well as cognitive impairment including an absence of language acquisition. High throughput exome sequencing identified a pathogenic variant in the UBE3A gene that was inherited from his asymptomatic mother. This variant causes the child to lose the contribution of the maternal allele, through loss of UBE3A genetic expression. UBE3A is localized into a genomic imprinting region which undergoes transcriptional regulation based on parental origin, an epigenetic phenomenon described in certain specific regions of the human genome. Its expression is repressed on the paternal chromosome at locus 15q11-13. The truncating variant on the maternal allele then leads to a complete loss of UBE3A expression. This results in Angelman syndrome. Angelman syndrome is a genetic neurodevelopmental disorder whose transmission mode depends on the causative molecular mechanism, which consists of a lack of contribution from the maternal 15q11-q13 region. Angelman's phenotype and evolution varies according to causative molecular mechanism. Precise laboratory diagnosis is especially important for genetic counselling: in our patient's family, the recurrence risk amounts to 50 % in the event of a future pregnancy, and the family's relatives must me informed and offered medical counsel.","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"83 4","pages":"470-472"},"PeriodicalIF":0.4,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144765810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Stability of hemostatic parameters in whole blood, fresh plasma, and frozen plasma: literature review and recommendations from the French Society of Thrombosis and Hemostasis (SFTH)]. [全血、新鲜血浆和冷冻血浆中止血参数的稳定性：文献综述和法国血栓与止血学会（SFTH）的建议]。

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Annales de biologie clinique Pub Date : 2025-08-26 DOI: 10.1684/abc.2025.1987

Claire Flaujac, Céline Delassasseigne, Marie-Françoise Hurtaud-Roux, Bénédicte Delahousse, Elodie Boissier, Céline Desconclois

引用次数: 0

[Parasitological examination of stools: french ANOFEL/LABAC recommendations]. [粪便寄生虫学检查：法国ANOFEL/LABAC建议]。

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Annales de biologie clinique Pub Date : 2025-08-26 DOI: 10.1684/abc.2025.1986

Xavier Iriart, Philippe Poirier, Damien Costa, Frédéric Dalle, Noémie Coron, Nicolas Argy, Loïc Favennec, Elodie Pernot-Marino, Stéphanie Haim-Boukobza, Jean-Marc Giannoli, Frédéric Gabriel, Florence Robert-Gangneux

引用次数: 0

Application value of serum miR-363 combined with arterial blood gas analysis parameters and lung ultrasound score in neonatal respiratory distress syndrome. 血清miR-363联合动脉血气分析参数及肺部超声评分在新生儿呼吸窘迫综合征中的应用价值。

IF 0.4

Annales de biologie clinique Pub Date : 2025-08-26 DOI: 10.1684/abc.2025.1985

Yanan Hou, Zhihua Liu, Zhiqiang Liu, Jing Mo, Lanjiao Chen, Yang Zhang

{"title":"Application value of serum miR-363 combined with arterial blood gas analysis parameters and lung ultrasound score in neonatal respiratory distress syndrome.","authors":"Yanan Hou, Zhihua Liu, Zhiqiang Liu, Jing Mo, Lanjiao Chen, Yang Zhang","doi":"10.1684/abc.2025.1985","DOIUrl":"10.1684/abc.2025.1985","url":null,"abstract":"Neonatal respiratory distress syndrome (NRDS) is caused by a deficiency of alveolar surface-active substances. The aim of this study was to investigate the value of miR-363 combined with arterial blood gas analysis parameters and lung ultrasound (LUS) score for diagnosis and prognostic assessment in NRDS. In this study, 104 neonates with NRDS and 76 healthy were included as subjects. RT-qPCR was used to detect the serum miR-363 expression. Neonatal artery blood was collected for arterial blood gas analysis and LUS score was performed on neonates. ROC curves were performed to analyze the diagnostic and prognostic predictive efficacy of single and combined indicators. Independent risk factors for development of NRDS and poor prognosis in neonates were analyzed by logistic analysis. Association of miR-363 with analysis indicators was assessed by Pearson correlation analysis. miR-363 expression was significantly lower in NRDS neonates than in healthy newborns. Compared to the healthy group, NRDS neonates had lower pH, PaO2, HCO3-, and BE levels, and higher PaCO2 levels and LUS scores, and the same trend was observed for the tests in the poor prognosis group. ROC curves indicated that the diagnostic efficacy of combined indicators was higher than that of single indicators. Logistic analysis showed that miR-363 was a risk factor for the development and poor prognosis of NRDS. Pearson correlation analysis suggested that miR-363 was positively correlated with pH, PaO2, HCO3-, and BE levels, and negatively correlated with PaCO2 levels and LUS scores. Serum miR-363 in combination with arterial blood gas analysis parameters and LUS scores may serve as a tool for diagnostic and prognostic risk assessment of NRDS, providing guidance for optimizing clinical strategies.","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"83 4","pages":"403-413"},"PeriodicalIF":0.4,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144777223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0