{"title":"[Evaluation of the analytical performances of the Atellica<sup>®</sup> DCA analyzer (Siemens Healthineers) for point of care determination of HbA<sub>1c</sub>].","authors":"Emmanuelle Guillard, Audrey Mourlin, Dany Mendes, Nathalie Leroy, Laetitia Florent, Philippe Gillery, Stéphane Jaisson","doi":"10.1684/abc.2025.1983","DOIUrl":"10.1684/abc.2025.1983","url":null,"abstract":"<p><p>The importance of HbA1c in the therapeutic monitoring of diabetic patients requires the use of robust and efficient assay methods, especially for point-of-care testing. This study evaluates the analytical performances of the Siemens Healthineers Atellica® DCA. After analyzing linearity and precision, the results were compared with those of the DCA Vantage (Siemens) and the Capillarys 3 Tera (Sebia, capillary electrophoresis). The main interferences (triglycerides and bilirubin) and the effect of total hemoglobin concentration were also assessed. The tests showed a good linearity over a range of 4.5 to 14 % HbA1c, and good precision with coefficients of variation ranging from 1.42 to 2.33 %. The correlation with capillary electrophoresis and the Atellica® DCA was excellent (r² = 0.991 and r² = 0.994, respectively). The accuracy of the results, assessed with external quality samples, was also satisfactory. The method is not sensitive to interference of hypertriglyceridemia up to 10 mmol/L or hyperbilirubinemia (up to 432 μmol/L), and provides reliable results even for low concentrations of total hemoglobin (down to 57,5 g/L). The Atellica® DCA also offers improved ergonomics, with a user-friendly touchscreen and can be connected to the laboratory's management software. this analyzer presents analytical performances suitable for use as point-of-care testing analyzer.</p>","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"83 4","pages":"395-402"},"PeriodicalIF":0.4,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144791030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The brain-gut axis: the dawn of neurological disorders?","authors":"Hailong Wu, Fengming Zhao, Wenzhi Jia, Peng Li","doi":"10.1684/abc.2025.1981","DOIUrl":"10.1684/abc.2025.1981","url":null,"abstract":"<p><p>Over the past two decades, the brain-gut axis has received increasing scholarly attention. However, few bibliometric analyses have systematically examined this area. We aimed to visualize the current status of research in this field, summarize hotspots, and present trends through a bibliometric analysis of published publications related to the brain-intestinal axis. Publications about the brain-gut axis were selected from the Web of Science Core Database (WoSCC) database. Countries/regions, institutions, authors, journals, references, and keywords in this field were visually analyzed using VOSviewer and CiteSpace software. A total of 3320 publications were eventually retrieved from 2000 to 2021. Publications on the brain-gut axis have increased year by year, especially in the last decade. The United States was the most prolific country (943, 28.404%), while Ireland was the country with the highest average number of article citations (156.03). Before 2011, most studies focused on the brain-gut axis, with irritable bowel syndrome being the most studied disorder; whereas in the last decade, most studies focused on the gut-brain axis, especially the microbial gut-brain axis, with neurodegenerative disorders being the most studied. In terms of global trends, research on the brain-gut axis is booming. Moreover, the role and therapeutic applications of the microbial-gut-brain axis in the progression of central nervous system (CNS) diseases, especially neurodegenerative diseases, are the focus of current research and future research trends.</p>","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"83 4","pages":"372-394"},"PeriodicalIF":0.4,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144762644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ludovic Bergon, Suzanne Burel, Olivier Dauwalder, Brigitte Rivière, Paul Cauquil, Mathilde Vernet, Bob Catala, Marie Isabelle Steinbach, Ibtihel Benlhaj, Josiane Boularan, Jérôme Garcia, Sarah Khatibi
{"title":"[Capnocytophaga canimorsus meningitis: no, the dog is not always man's best friend!]","authors":"Ludovic Bergon, Suzanne Burel, Olivier Dauwalder, Brigitte Rivière, Paul Cauquil, Mathilde Vernet, Bob Catala, Marie Isabelle Steinbach, Ibtihel Benlhaj, Josiane Boularan, Jérôme Garcia, Sarah Khatibi","doi":"10.1684/abc.2025.1989","DOIUrl":"10.1684/abc.2025.1989","url":null,"abstract":"<p><p>Capnocytophaga canimorsus meningitis are rare invasive infection with unpredictable outcomes. Therefore, rapid diagnosis and treatment are crucial. Here, we describe a case with an initially falsely reassuring clinical presentation. Lumbar puncture reveals purulent and inflammatory cerebrospinal fluid, but the syndromic multiplex PCR remains negative. The challenging microbiological diagnosis resulted from a meticulous examination of the CSF and the performance of a 16S rRNA PCR followed by sequencing. A thorough medical history, including close contact with dogs and identification of immunosuppression factors, is critical for diagnosis, emphasizing the importance of clinical-biological collaboration.</p>","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"83 5","pages":"1-6"},"PeriodicalIF":0.4,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144877166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Seraphin Ahoui, Joseph S Godonou, Giovanna Zossoungbo, Missikè Melikan, Aimé Vinasse, Jules Akotan, Astride Finkpon, Carine Pedro, Bruno L Agboton, Jacques Vigan
{"title":"[Determinants of hymerkalamia among patients with kidney failure undergoing conservative care at the national teaching hospital of Cotonou (Bénin)].","authors":"Seraphin Ahoui, Joseph S Godonou, Giovanna Zossoungbo, Missikè Melikan, Aimé Vinasse, Jules Akotan, Astride Finkpon, Carine Pedro, Bruno L Agboton, Jacques Vigan","doi":"10.1684/abc.2025.1992","DOIUrl":"10.1684/abc.2025.1992","url":null,"abstract":"<p><p>Hyperkalemia, characterized by an elevated serum potassium level, is a common and serious complication in patients with Chronic Kidney Disease (CKD). It can lead to major cardiovascular disorders, making rigorous management essential. Objective: To study the determinants of hyperkalemia in patients with renal failure undergoing conservative treatment followed at the CNHU-HKM in Cotonou (Bénin) in 2024. This was a cross-sectional study conducted from October 1 to December 31, 2024, involving patients monitored in the teaching nephrology and hemodialysis clinic for end-stage chronic kidney disease. Eligible patients were at least 18 years old, on conservative treatment, and had provided informed consent. The dependent variable was a serum potassium level above 5.0 mmol/L associated with a cardiac disorder. Determinants were identified using a significance threshold of p < 0.05. A total of 110 patients were included. The mean age was 57 ± 1.3 years, with a sex ratio of 1.8. Among them, 30 patients developed hyperkalemia, representing a prevalence of 27.3 %. The identified determinants were male gender (aOR = 5.3; p = 0.024) and the use of ACE inhibitors (aOR = 5.1; p = 0.022). Hyperkalemia remains a frequent issue in nephrology, requiring increased vigilance and appropriate management, particularly for at-risk patients.</p>","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"83 5","pages":"1-9"},"PeriodicalIF":0.4,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144877167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"BIA-ALCL diagnosis: the relevance of cytology and flow cytometry in periprosthetic fluid analysis.","authors":"Radu Chiriac, Marie Donzel, Lucile Baseggio","doi":"10.1684/abc.2025.1976","DOIUrl":"10.1684/abc.2025.1976","url":null,"abstract":"<p><p>Breast implant-associated anaplastic large cell lymphoma (BIA-ALCL) is a rare T-cell non-Hodgkin lymphoma, representing less than 1% of breast neoplasms. Despite its low incidence, the increasing number of women with breast implants necessitates vigilance among clinicians and pathologists. BIA-ALCL presents in situ and invasive forms, with varying prognoses. The National Comprehensive Cancer Network guidelines recommend cytology, immunohistochemistry, and flow cytometry (FCM) for diagnosis. This retrospective study analyzed 16 periprosthetic fluid (PF) samples from suspected lymphoma cases between January 2018 and January 2024. Cytological and immunological analyses were performed using May Grünwald-Giemsa staining, and FCM with BD FACSCanto II and BD LYRIC cytometers. A 10 or 12-colour FCM panel including pan-T markers and CD30 was used. Of the 16 samples, 2 cases were diagnosed with BIA-ALCL. BIA-ALCL cases showed atypical CD4+ T-cells with large size and loss of CD3 and CD7. In contrast, the 14 reactive cases did not exhibit atypical cells. Key challenges included sample volume limitations, cell dilution, and distinguishing neoplastic cells from reactive ones, particularly in cases with low cell counts. FCM, combined with an extensive panel of pan-T markers and CD30, effectively differentiates anaplastic BIA-ALCL cells from reactive seroma. However, it should complement, not replace, traditional diagnostic methods. Recognizing pitfalls and correlating FCM findings with clinical and morphological data enhances diagnostic accuracy. FCM is a valuable tool for diagnosing BIA-ALCL but should be used alongside other methods. Accurate diagnosis depends on understanding sample-specific challenges and integrating FCM results with clinical context.</p>","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"83 3","pages":"303-309"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yazid El Alaoui Boufares, Grégoire Pasquier, Laurence Lachaud
{"title":"Strongyloides stercoralis hyperinfection syndrome: case report of therapeutic failure with ivermectin.","authors":"Yazid El Alaoui Boufares, Grégoire Pasquier, Laurence Lachaud","doi":"10.1684/abc.2025.1968","DOIUrl":"10.1684/abc.2025.1968","url":null,"abstract":"<p><p>Strongyloidiasis (or anguillulosis) is helminthosis caused by Strongyloides stercoralis found mostly in (sub)tropical areas and sometimes in temperate settings. We report a case of strongyloidiasis hyperinfection, diagnosed on a BALF in a 46-year-old woman of senegalese origin with no recent travel history. The patient initially presented to the emergency department with worsening chronic lower back pain, asthenia and a 10-day history of general deterioration. Plasma cell leukemia was diagnosed and treated with a block of dexamethasone combined with chemotherapy. Our laboratory incidentally detected the presence of Strongyloides larvae in a BAL specimen. The patient received a prophylactic dose of Ivermectin prior to the administration of high doses of corticosteroids. Once the diagnosis of hyperinfestation syndrome had been established, curative doses of Ivermectin were administered, initially by nasogastric tube and then subcutaneously. The evolution was rapidly unfavorable, with the appearance of edema and cerebral hemorrhages which led to the patient's death 10 days later.</p>","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"83 3","pages":"321-323"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144044676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Vérification des performances de la méthode COBAS® AmpliPrep/COBAS® TaqMan HIV-1 test, v 2.0 pour la quantification de la charge virale plasmatique du VIH-1.","authors":"Ella Christelle Brou, Aboubakar Touré, Fidèle Sounan Touré, Eugénie Yaudé, Inza Dosso, Marcellin Konan, Durand Sery, Christiane Kiki-Barro","doi":"10.1684/abc.2025.1972","DOIUrl":"10.1684/abc.2025.1972","url":null,"abstract":"<p><p>The laboratory of the National Institute of Public health makes HIV-1 plasma RNA quantification. That test is required to follow up HIV patient on ART (Antiretroviral therapy). The laboratory was accredited by the West African Accreditation System (SOAC) in 2022 for HIV real time PCR. Here we report the experience of the laboratory for verifiying performance of the COBAS® AmpliPrep/COBAS® TaqMan (CAP/CTM) HIV-1 test, v 2.0 kit for measuring viral load by real time PCR. According with the technical guides of the French Accreditation Committee (COFRAC) and the recommendations of the French Society of Clinical Biology (SFBC), different steps were followed. These are the risk assessment, the study of reliability, intermediate precision, accuracy and precision of the method, estimation of measurement uncertainty and comparison of methods. Coefficient of variation for repeatability, reproducibility and trueness were within target of 5 %. The results from the CAP/CTM were reproductible and identical to those of the COBAS 6800. The COBAS® AmpliPrep/COBAS® TaqMan HIV-1 Test, v 2.0 method is suitable for routine laboratory use and has analytical performance for its intended utility, which mean the assessment of disease progression and monitoring of antiretroviral therapy for HIV-1 patients.</p>","PeriodicalId":93870,"journal":{"name":"Annales de biologie clinique","volume":"83 3","pages":"310-320"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}