Abdullah Karasu, Yağmur Kuşcu, Caner Kayikci, Serkan Yildirim, Oğuzhan Kuşcu, Metin Kiliçlioğlu
{"title":"Effect of low- and high-dose methotrexate on wound healing in rats.","authors":"Abdullah Karasu, Yağmur Kuşcu, Caner Kayikci, Serkan Yildirim, Oğuzhan Kuşcu, Metin Kiliçlioğlu","doi":"10.1590/acb403225","DOIUrl":"10.1590/acb403225","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the effect of intraperitoneal treatment with low- and high-dose methotrexate (MTX) on wound healing in rats.</p><p><strong>Methods: </strong>The study sample consisted of 54 healthy rats. Under aseptic conditions, skin wounds were created with two circular full-thickness punch tools, 10 mm in diameter, one on the right and the other one on the left of the dorsal vertebral line. The rats were randomly assigned to one of three main treatment groups. On the 0th day (2 hours before wound creation), 7th day, and 14th day, the control group received 0.3-mL saline, the low-MTX group received 3 mg/kg MTX, and the high-MTX group received 30 mg/kg MTX, all administered intraperitoneally. The wounds were evaluated seven, 14, and 21 days after injury through morphometrical, biochemical, histopathological, and immunohistochemical analyses.</p><p><strong>Results: </strong>MTX dose-dependently decreased the degree of inflammation and angiogenesis, tissue hydroxyproline level, and HSP70 and tumor necrosis factor-α expression in the early phase of wound healing. It also suppressed epithelialization and collagen 1 expression throughout the wound-healing process.</p><p><strong>Conclusion: </strong>The wounds treated with high-dose of MTX had statistically delayed wound closure on days 7, 14 and 21 compared to the saline group, while wounds treated with low-dose of MTX only had statistically delayed wound closure on day 14. In addition, weight loss was observed in rats treated with high-dose MTX, which was thought to reflect its toxicity. The dose-dependent adverse effect of MTX on wound healing may be due to its antiproliferative, antifibrotic, anti-inflammatory, and antiangiogenic effects.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e403225"},"PeriodicalIF":0.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11908735/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of borneol on apoptosis of hypoxia/reoxygenation H9c2 cells and myocardial ischemia-reperfusion injury rats.","authors":"Hui Zhang, Junfang Dong, Jianwu Zhang, Hongxue Chen, Ting Liu, Ruixue Gan, Jing Wen, Yangyou Li","doi":"10.1590/acb402225","DOIUrl":"10.1590/acb402225","url":null,"abstract":"<p><strong>Purpose: </strong>To explore the protective effects of borneol in myocardial ischemia-reperfusion injury (MIRI) and the mechanism of apoptosis.</p><p><strong>Methods: </strong>Cell viability was detected by CCK-8. The total superoxide dismutase (T-SOD) and lactate dehydrogenase (LDH) leakage of cells were tested by biochemical assay kit. Detection of apoptosis was by flow cytometry. Serum levels of creatine kinase isoenzyme MB (CK-MB), LDH, and cardiac troponin I (cTnI) were detected by enzyme-linked immunosorbent assay. Myocardial infarction area and pathological changes were observed via 2,3,5-triphenyltetrazolium chloride (TTC) staining and hematoxylin and eosin staining. The expressions of apoptosis-related proteins in cells and myocardial tissues were detected by Western blot.</p><p><strong>Results: </strong>H9c2 cell viability was significantly increased by pretreatment with 16 and 32 μg/mL of borneol. Borneol pretreatment significantly increased the T-SOD levels and reduced LDH leakage and apoptosis. In MIRI rats, borneol pretreatment significantly reduced serum levels of CK-MB, LDH and cTnI, decreased myocardial infarction area, and improved myocardial injury in different degree. Western blot results showed that borneol pretreatment significantly reduced the expression of Bcl-2-associated X protein (Bax) and Cysteine-aspartate protease-3 (Caspase-3) in cells and myocardial tissues of rats.</p><p><strong>Conclusion: </strong>Borneol can protect myocardial injury cells and mitigate MIRI by inhibiting cardiomyocyte apoptosis.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e402225"},"PeriodicalIF":0.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11908740/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Silvio Pires Gomes, Gabriela Salim de Castro, Vinicius Pedro Silva de Oliveira, Bruno Cogliati, Andressa Galvão da Silva Iacopino, Ivanir Santana de Oliveira Pires, Bruno Cesar Schimming, Fernanda Gosuen Gonçalves Dias, José Roberto Kfoury Junior, Tais Harumi de Castro Sasahara
{"title":"Hepatic stereological analysis in obese Zucker rats (Leprfa) with dyslipidemia.","authors":"Silvio Pires Gomes, Gabriela Salim de Castro, Vinicius Pedro Silva de Oliveira, Bruno Cogliati, Andressa Galvão da Silva Iacopino, Ivanir Santana de Oliveira Pires, Bruno Cesar Schimming, Fernanda Gosuen Gonçalves Dias, José Roberto Kfoury Junior, Tais Harumi de Castro Sasahara","doi":"10.1590/acb402325","DOIUrl":"10.1590/acb402325","url":null,"abstract":"<p><strong>Purpose: </strong>To characterize histologically and stereologically the hepatic steatosis in obese Zucker (fat, n = 6, with a mutation in the leptin receptor - Leprfa) and control Zucker (lean, n = 6) rats, analyzing macroscopic and microscopic differences to understand the influence of obesity on hepatic pathology.</p><p><strong>Methods: </strong>Zucker rats were fed standard chow for 90 days. Macroscopic, qualitative, and histoquantitative (stereological) approaches were used, involving body and liver weight measurement, morphological analysis, and histopathological classification of metabolic dysfunction-associated steatotic liver disease.</p><p><strong>Results: </strong>Zucker fat rats had higher body weight (p = 0.0022), liver weight (p = 0.0022), serum total cholesterol (p = 0.0022), and triacylglycerol (p = 0.0022) compared to Zucker lean rats. Stereological analysis showed that hepatocyte volume density (p = 0.0022) and total hepatocyte volume (p = 0.0001) were lower, and the volume density (p = 0.002) and total volume of steatosis (p = 0.002) were higher in Zucker fat rats compared to lean rats.</p><p><strong>Conclusion: </strong>The findings indicated that obesity induces significant alterations in the hepatic morphology of Zucker rats, showing that hepatocyte volume is lower in obese animals. This study reinforces the utility of the obese Zucker rat model to investigate the effects of obesity on liver health and suggests hepatic steatosis requires therapeutic strategies focused on modulating these parameters.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e402325"},"PeriodicalIF":0.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11908736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sofia Wagemaker Viana, Ayla Gerk, Sofia Schmitt Schlindwein, Enzzo Marrazzo, Brenda Feres, Lívia Ribeiro, Madeleine Carroll, David Patrick Mooney, Gabriel Schnitman, Cristina Pires Camargo
{"title":"Public health system expenditure on motor vehicle collisions in Brazil: an ecological study.","authors":"Sofia Wagemaker Viana, Ayla Gerk, Sofia Schmitt Schlindwein, Enzzo Marrazzo, Brenda Feres, Lívia Ribeiro, Madeleine Carroll, David Patrick Mooney, Gabriel Schnitman, Cristina Pires Camargo","doi":"10.1590/acb402525","DOIUrl":"10.1590/acb402525","url":null,"abstract":"<p><strong>Purpose: </strong>To assess the cost of traffic accidents in Brazil and the impact of age/location.</p><p><strong>Methods: </strong>All patients admitted to a Brazilian hospital due to traffic accidents from January 2012 to December 2022 and cost of hospital services were obtained from the Department of Information Technology of the Unified Health System. Demographic data were collected in the Brazilian Institute of Geography and Statistics database. Parametric and nonparametric data were analyzed. The Kruskal-Wallis' test and a post-hoc test were used for data comparison. The ARIMA linear regression method for trend estimation.</p><p><strong>Results: </strong>In Brazil, 1.6 million individuals were involved in traffic accidents between 2012-2022, resulting in a cumulative hospital expenditure of US$ 38 million. The average hospital admission cost during this time was US$ 239.66, but no correlation was found between the cost per capita and driver population density increase. Hospitalization in the Midwest/South was higher.</p><p><strong>Conclusion: </strong>The economic impact of traffic accidents on the Brazilian public health system is significant. With a high number of victims admitted annually and evident regional and age-related disparities, there is a clear need for comprehensive and cost-effective healthcare strategies.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e402525"},"PeriodicalIF":0.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11908734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Evandro Luis da Cunha Oliveira, Yvone Avalone de Moraes Villela de Andrade Vicente
{"title":"Study of the enteric and motor inervation, pelvic musculature, and alterations in the sacral region of rat fetuses with ethylenethiourea-induced anorectal anomaly.","authors":"Evandro Luis da Cunha Oliveira, Yvone Avalone de Moraes Villela de Andrade Vicente","doi":"10.1590/acb401525","DOIUrl":"10.1590/acb401525","url":null,"abstract":"<p><strong>Purpose: </strong>The aims of this work were to induce anorectal anomaly in rat fetuses via the planned administration of ethylenethiourea (ETU), and to study fetuses exhibiting anorectal malformation, as well as apparently normal fetuses submitted to the effect of ETU.</p><p><strong>Methods: </strong>Time-mated pregnant Wistar rats were randomly divided into control and experimental groups. On gestational day 10, the experimental group received 10% ETU (130 mg/kg) by gavage, whereas the control rats received vehicle only. The embryos were harvested by cesarean section on gestational day 21. The fetuses exposed to ETU were divided into two groups: affected (without any clear anorectal alterations); and the malformed (with anorectal anomaly). The neuromotor plates were identified by immunohistochemistry with acetylcholinesterase, and alterations in the sacral region were evaluated by histological and morphometric studies.</p><p><strong>Results: </strong>We used 43 control fetuses, 82 affected fetuses, and 118 malformed fetuses in this study. The most frequent associated macroscopic anomalies were spina bifida (55 fetuses), encephalocele (20), and alterations in the lower limbs (5). The sacroiliac was malformed in 45% of the affected fetuses and in 53.2 % of the malformed fetuses.</p><p><strong>Conclusion: </strong>ETU leads to a reduced number of motor neurons in the pelvic musculature of both the malformed and the affected rats. The enteric neurons are altered in the malformed fetuses, but not in the affected ones. Both the affected and malformed rats exhibit sacral alterations that do not interfere with neurons.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e401525"},"PeriodicalIF":0.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11908733/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Naely Nobre Costa, Jennifer Ferreira Dos Santos, Maria Fernanda de Almeida Cavalcante Aranha, Erik William Farias Coelho, Victor Leno Silva Paes, Rita de Cássia Silva de Oliveira
{"title":"Repair methods in peripheral nerves after traumatic injuries: a systematic literature review.","authors":"Naely Nobre Costa, Jennifer Ferreira Dos Santos, Maria Fernanda de Almeida Cavalcante Aranha, Erik William Farias Coelho, Victor Leno Silva Paes, Rita de Cássia Silva de Oliveira","doi":"10.1590/acb401225","DOIUrl":"10.1590/acb401225","url":null,"abstract":"<p><strong>Purpose: </strong>To identify and describe the most used surgical repair methods for traumatic injuries to peripheral nerves, as well as highlight the causes of trauma to peripheral nerves and the most prevalent traumatized nerves.</p><p><strong>Methods: </strong>This is a systematic literature review using the recommendations of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). The searches were carried out in PubMED, in the time window from January 2018 to December 2022.</p><p><strong>Results: </strong>In total, 3,687 articles were collected, of which, after applying the inclusion and exclusion filters and analyzing the risk of bias, 34 articles remained. It was observed that the age of the injury and type of nerve repair strongly influence the recovery of patients. The most identified trauma repair procedures were neurolysis, direct suturing, grafting, and nerve transfer. Among these four procedures, direct suturing is currently preferred.</p><p><strong>Conclusion: </strong>Several repair methods can be used in peripheral nerve injuries, with emphasis on direct suturing. However, nerve transfer proves to be a differential in those cases in which repair is delayed or the first treatment options have failed, which shows that each method will be used according to the indication for each case.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e401225"},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11849802/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143506688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maressa Daniela Anghinoni Bonissoni, Fernanda Mara Alves, Rafaella Frederico Almeida, Lucca Smaniotto, Maria Paula de Andrade Berny, Victor Pereira da Silva, Brenda Stephanie Fiuza, Guilherme Cirino Rodrigues, Eloisa Maria Neres, Rodrigo Kern, Carlos Victor Pereira Dos Santos, Janoário Athanazio de Souza, Daniel Rech, Carolina Panis
{"title":"Factors associated with recurrence in operable breast cancer patients undergoing surgery as the sole treatment modality.","authors":"Maressa Daniela Anghinoni Bonissoni, Fernanda Mara Alves, Rafaella Frederico Almeida, Lucca Smaniotto, Maria Paula de Andrade Berny, Victor Pereira da Silva, Brenda Stephanie Fiuza, Guilherme Cirino Rodrigues, Eloisa Maria Neres, Rodrigo Kern, Carlos Victor Pereira Dos Santos, Janoário Athanazio de Souza, Daniel Rech, Carolina Panis","doi":"10.1590/acb402025","DOIUrl":"10.1590/acb402025","url":null,"abstract":"<p><strong>Purpose: </strong>Approximately 10% of breast cancer patients with localized operable disease experience recurrence. This study aimed to evaluate patients with early-stage breast cancer (TNM I and II) who underwent surgery without the need for neoadjuvant therapy.</p><p><strong>Methods: </strong>The total of 117 patients was included in the study: 104 without recurrence and 13 with recurrence. We analyzed various clinicopathological parameters, including body mass index, age at diagnosis, menopausal status, pesticide exposure, and tumor characteristics such as HER2 amplification, Ki67 index, molecular subtype, and histological grade.</p><p><strong>Results: </strong>Two primary subsets were identified as being associated with disease recurrence. The first subset (PC1) was characterized by HER2 amplification and metastatic disease, while the second subset (PC2) comprised premenopausal young women exposed to pesticides who had triple-negative tumors. While some of these factors are well-documented in the literature, pesticide exposure emerged as a notable regional factor contributing to poor outcomes in breast cancer patients.</p><p><strong>Conclusion: </strong>These findings underscored the significance of identifying local and regional risk factors when assessing recurrence risk in women with breast cancer, particularly in cases in which surgery is the primary treatment approach.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e402025"},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11849801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143506683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Analysis of clinical and pathological prognostic factors of survival in rectal adenocarcinoma treated with preoperative radiochemotherapy.","authors":"Sarhan Sydney Saad, Nora Forones, Gaspar Lopes Filho, Jaques Waisberg, Elesiario Caetano Júnior, Ricardo Artigiani-Neto, Delcio Matos","doi":"10.1590/acb401125","DOIUrl":"10.1590/acb401125","url":null,"abstract":"<p><strong>Purpose: </strong>To identify the prognostic variables related to the survival of patients operated on for adenocarcinoma of the rectum who underwent preoperative radiochemotherapy (RCT).</p><p><strong>Methods: </strong>We studied 70 patients from the Discipline of Surgical Gastroenterology at Escola Paulista de Medicina from 2000 to 2019, with rectal cancer located up to 10 cm from the anal verge and with stages II or III, submitted to preoperative RCT and curative surgery (R0) and with follow-up of at least 12 months. Clinical restaging was performed four to six weeks after the end of neoadjuvant treatment to characterize the degree of clinical tumor regression. Surgery by laparotomy or videolaparoscopy was performed six to 12 weeks after RCT. Primary endpoint were: overall survival (OS), disease-free survival (DFS), metastasis-free survival (MSS), and neoplasm-specific survival (SEN). These were compared with gender, age, carcinoembryonic antigen (CEA) dosage, distance from the tumor to the anal verge, radiation dose, radiotherapy-surgery interval, clinical regression, type of surgery, pT and pN TNM stage tumor, number of nodes, circumferential resection margin, and complete pathological response. Survival was assessed by Kaplan-Meier curves. Univariate and multivariate Cox analyses were calculated to identify factors associated with survival outcomes.</p><p><strong>Results: </strong>The mean follow-up time was 62 months. The pathological complete response rate was 18.6%. Univariate cox regression showed a significant relationship of CEA equal to or greater than 4 ng/mL with DFS and MFS, pT3/pT4 staging with DFS, MFS and SEN, pN1/N2 with DFS, MFS and SEN and stages II and III with DFS and MFS. Multivariate regression found that CEA, pT, and pN staging are independent prognostic factors for DFS, MFS, and SEN.</p><p><strong>Conclusion: </strong>Carcinoembryonic antigen level prior to radiotherapy, pT staging and pN staging were independent prognostic factors for survival in patients with rectal adenocarcinoma who are treated with preoperative radiochemotherapy.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e401125"},"PeriodicalIF":0.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11810072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143400990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}