Effects of borneol on apoptosis of hypoxia/reoxygenation H9c2 cells and myocardial ischemia-reperfusion injury rats.

Acta cirurgica brasileira Pub Date : 2025-03-14 eCollection Date: 2025-01-01 DOI:10.1590/acb402225

Hui Zhang, Junfang Dong, Jianwu Zhang, Hongxue Chen, Ting Liu, Ruixue Gan, Jing Wen, Yangyou Li

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Abstract

Purpose: To explore the protective effects of borneol in myocardial ischemia-reperfusion injury (MIRI) and the mechanism of apoptosis.

Methods: Cell viability was detected by CCK-8. The total superoxide dismutase (T-SOD) and lactate dehydrogenase (LDH) leakage of cells were tested by biochemical assay kit. Detection of apoptosis was by flow cytometry. Serum levels of creatine kinase isoenzyme MB (CK-MB), LDH, and cardiac troponin I (cTnI) were detected by enzyme-linked immunosorbent assay. Myocardial infarction area and pathological changes were observed via 2,3,5-triphenyltetrazolium chloride (TTC) staining and hematoxylin and eosin staining. The expressions of apoptosis-related proteins in cells and myocardial tissues were detected by Western blot.

Results: H9c2 cell viability was significantly increased by pretreatment with 16 and 32 μg/mL of borneol. Borneol pretreatment significantly increased the T-SOD levels and reduced LDH leakage and apoptosis. In MIRI rats, borneol pretreatment significantly reduced serum levels of CK-MB, LDH and cTnI, decreased myocardial infarction area, and improved myocardial injury in different degree. Western blot results showed that borneol pretreatment significantly reduced the expression of Bcl-2-associated X protein (Bax) and Cysteine-aspartate protease-3 (Caspase-3) in cells and myocardial tissues of rats.

Conclusion: Borneol can protect myocardial injury cells and mitigate MIRI by inhibiting cardiomyocyte apoptosis.

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冰片对缺氧/再氧合H9c2细胞凋亡及心肌缺血再灌注损伤的影响。

目的：探讨冰片对心肌缺血再灌注损伤（MIRI）的保护作用及凋亡机制。方法：采用CCK-8法检测细胞活力。采用生化检测试剂盒检测细胞总超氧化物歧化酶（T-SOD）和乳酸脱氢酶（LDH）渗漏。流式细胞术检测细胞凋亡。采用酶联免疫吸附法检测血清肌酸激酶同工酶MB （CK-MB）、LDH和心肌肌钙蛋白I （cTnI）水平。采用2,3,5-三苯基四氯化铵（TTC）染色和苏木精、伊红染色观察心肌梗死面积及病理变化。Western blot检测细胞和心肌组织中凋亡相关蛋白的表达。结果：16、32 μg/mL冰片预处理能显著提高H9c2细胞活力。冰片预处理可显著提高T-SOD水平，减少LDH渗漏和细胞凋亡。在MIRI大鼠中，冰片预处理可显著降低血清CK-MB、LDH、cTnI水平，减少心肌梗死面积，不同程度改善心肌损伤。Western blot结果显示，冰片预处理显著降低大鼠细胞和心肌组织中bcl -2相关X蛋白（Bax）和半胱氨酸-天冬氨酸蛋白酶-3 （Caspase-3）的表达。结论：冰片对心肌损伤细胞具有保护作用，可通过抑制心肌细胞凋亡减轻MIRI。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Acta cirurgica brasileira

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