JSFA reports最新文献

{"title":"Inventory of Loranthaceae parasites of Bayangam fruit trees in West Cameroon","authors":"Gilles Jiope Azangue,&nbsp;Bonaventure Sonke,&nbsp;Wauffo Fokom David","doi":"10.1002/jsf2.209","DOIUrl":"https://doi.org/10.1002/jsf2.209","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Loranthaceae parasitism is a limiting factor for crops as well as many other plant diseases and pests. The present study was conducted to make an inventory of Loranthaceae present on fruit trees species grown in the Bayangam locality. It also aimed to record the damage caused by these pests on the host plants. To achieve this, 40 transects of 100 m length and 50 m width were set up in the locality of Bayangam. Observations were made on the presence or absence of the parasites on the host plants, the number of Loranthaceae clumps carried by each parasitized tree, the taxon of the parasite, and the damage caused by the Loranthaceae on the host plants.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The inventories identified six species of Loranthaceae belonging to four genera. These were <i>Globimetula braunii</i>, <i>Globimetula dinklagei</i>, <i>Globimetula oreophila</i>, <i>Helixanthera mannii</i>, <i>Phragmanthera capitata</i>, and <i>Tapinanthus globiferus</i>. Parasite densities varied among species and increased with the circumference of host plants. <i>Globimetula braunii</i> and <i>Phragmanthera capitata</i> were the most invasive species on host plants.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Fruit trees in Bayangam are largely parasitized by Loranthaceae. The only control method used by the populations is pruning of parasitized branches during drought periods. This method is not effective because of the large number of trees and branches parasitized. The identification of Loranthaceae-resistant cultivars in fruit tree production is an interesting element in the perspective of control.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"4 6","pages":"269-275"},"PeriodicalIF":0.0,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jsf2.209","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141488420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Casein-based nanocarriers for encapsulation of propolis extract: Design, fabrication, and characterization","authors":"Javad Feizy,&nbsp;Mansooreh Soleimanifard,&nbsp;Francesca Maestrelli","doi":"10.1002/jsf2.205","DOIUrl":"https://doi.org/10.1002/jsf2.205","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Propolis exhibits multiple biological and pharmacological properties attributed to the presence of natural bio active compounds. In spite of its potential healthy effects, its use in health-food and pharmaceutical products is very restricted due to its intense aroma, highly unstable, low aqueous solubility, and low bioavailability. The purpose of this study is to fabricate an appropriate, stable, and biodegradable casein-based nanocarrier as propolis delivery system. Propolis-loaded casein nanocarriers were prepared at different propolis extract/caseinate ratio and assessed for physicochemical, structural, and thermal properties.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The nanocarriers showed an increase of particle size augmenting propolis extract/caseinate ratio and caseinate concentration. Image processing studies revealed an increase in <i>L</i>* parameter (89.743), while <i>b</i>* parameter revealed a reduction in the yellow color (14.655) increasing the amount propolis extract in the nanocarrier. Surface photomicrographs evidenced that an increment of propolis extract decreased the network compactness of the nanocarriers correlated with the lower entrapment of propolis extract into carriers at higher propolis extract/caseinate ratio. X-ray diffraction pattern suggested that propolis encapsulation produced a decrement in the caseinate crystallinity while differential scanning calorimetry and thermogravimetric/differential thermal analysis thermograms evidenced an increment of thermal stability of nanocarrier with increasing propolis extract content.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Propolis extract encapsulated within casein nanocarriers represented convenient physicochemical attributes and could provide as bioactive load in food/medical system.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"4 6","pages":"258-268"},"PeriodicalIF":0.0,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jsf2.205","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141488498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the bioactive properties, tocopherol and phenolic components of germinated, roasted, and boiled chufa (Cyperus esculentus) tubers","authors":"Mehmet Musa Özcan,&nbsp;Havvanur Yılmaz","doi":"10.1002/jsf2.207","DOIUrl":"https://doi.org/10.1002/jsf2.207","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Chufa flour can be used as a supplement to cereal flour to increase its calcium content. Many studies have shown that chufa tubers have high nutritional value.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The carotenoid contents of the tubers ranged from 0.270 (roasting) to 1.897 μg/g (germination). The total flavonoid contents of chufa tuber were recorded between 4.60 and 17.93 mg CE/100 g. According to the results obtained, oil yield was determined between 12.55% (germination) and 18.05% (roasting). The protein content of the chufa tuber was found to be 16.60% in the germinated sample with the highest value. Potassium (3792.67–8384.85), sulfur (975.74–1804.01), phosphorus (944.08–1256.78), and magnesium (504.45–672.82 ppm) are the main inorganic components of ash in the samples examined. The highest carotenoid content in the oil was observed in the germinated sample with 7084.10 μg/g, total phenol content 9.44 mgGAE/100 g, total flavonoid content 561.10 mg CE/100 g, and antioxidant activity value 32.39%. It was determined that oleic acid (70.95%–73.47%) was the dominant fatty acid. The results of tocopherol content of chufa tuber oil belong to the highest α-tocopherol by boiling (8.91 mg/kg), roasting (9.07 mg/kg), and germinating (9.64 mg/kg).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>According to the results of the study, the germination process applied to the chufa tuber increased the protein and macro mineral content. The germination process applied to the chufa tuber increased the content of unsaturated fatty acids and tocopherol. Chufa tuber oil contains highly unsaturated fatty acids, vitamin E and phenolic compounds. Due to its nutritional quality, chufa oil is recommended for consumption and industrial use.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"4 5","pages":"243-254"},"PeriodicalIF":0.0,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jsf2.207","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141182134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of biogas production and bacterial load from co-digestion of chicken manure with different types of household waste","authors":"Mohammad Enamul Haque,&nbsp;Roman Ryndin,&nbsp;Heinz-Peter Mang,&nbsp;Humayun Kabir,&nbsp;Mohammad Alimul Islam","doi":"10.1002/jsf2.206","DOIUrl":"https://doi.org/10.1002/jsf2.206","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The aim of this study was to evaluate the biogas production of chicken manure (CM) co-digestion with different types of household waste (soft organic [SO] and food waste [FW]), as well as to evaluate the bacterial load of feeding stock and digested slurry samples before and after anaerobic digestion (AD). The experiment was carried out using lab-based prototype digesters for co-digestion of CM with different household wastes (5%). Three experimental groups (T1, T2, and T3) were designed using mixing ratios of SO:CM:H₂O:inoculum (5:22.5:22.5:50), FW:CM:H₂O:inoculum (5:22.5:22.5:50), and (SO + FW):CM:H₂O:inoculum (2.5 + 2.5:22.5:22.5:50). The digesters were set at 28–34°C for 30 days for hydraulic retention time (HRT). Total viable count (TVC), <i>Escherichia coli</i>, and <i>Salmonella</i> spp. counts were determined using the spread plate technique.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The study revealed that the highest average cumulative biogas yield was achieved from T1 &gt; T3 &gt; T2, but the concentration of CH₄ was found in T3 &gt; T2 &gt; T1. The biogas production between the three groups was statistically nonsignificant (<i>p &gt;</i> 0.05) but the daily concentration of CH₄ was found statistically significant (<i>p &lt;</i> 0.05). The average concentration of CH₄ and CO₂ in biogas was found to be 30% and 68% for T1, 60% and 37% for T2, and 69% and 27% for T3. However, the H₂S content was within the acceptable range. The bacterial load was decreased by 2–3 logs before and after AD, and this reduction was statistically significant (<i>p &lt;</i> 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The research found that the co-digestion of CM with combined household wastes increased the methane concentration in biogas.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"4 5","pages":"235-242"},"PeriodicalIF":0.0,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jsf2.206","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141182135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regenerative agriculture: The science, economics and practice","authors":"Lucy Wright","doi":"10.1002/jsf2.191","DOIUrl":"https://doi.org/10.1002/jsf2.191","url":null,"abstract":"","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"4 4","pages":"188-190"},"PeriodicalIF":0.0,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140651233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential QTL underlie wheat grain physical quality when measured using image-based versus traditional laboratory methods","authors":"Livinus Emebiri,&nbsp;Shane Hildebrand","doi":"10.1002/jsf2.192","DOIUrl":"10.1002/jsf2.192","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The marketing value of wheat (<i>Triticum aestivum</i> L.) is determined, in parts, by the grain's physical characteristics, owing to which they directly (or indirectly) influence milling performance and baking quality. These characteristics have been manually measured in the past, but now, digital image analysis (DIA) is being increasingly used to replace the slow phenotyping system. Here, we asked whether this could lead to the identification of the same or different genes when compared to the traditional phenotyping methods.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>We measured grain physical quality on 142 wheat doubled haploids grown in the field over 2 years, and in using the quantitative trait locus (QTL) mapping approach we found that (1) for wheat grain weight, the use of DIA provided genetic information that mostly conformed to those obtained using the traditional phenotyping methods, with heritability estimates that were identical across both methods. Majority of the QTL detected were consistent between the traditional versus digital phenotyping methods; (2) a more complex architecture, however, arose from QTL analyses of hectoliter mass (HLM) and percentage of shriveled grains (SCR). The estimates for heritability varied by as much as 0.24 across methods and, more significantly, many of the detected QTL for both traits were method-specific; (3) though method-specific, identified QTL was mapped to genomic regions known to harbor genes for grain physical traits.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Thousand-grain weight (TGW) is robust to a phenotyping method, but a different genetic system underlies HLM and SCR, when these were measured using traditional versus digital image analysis. For these traits, heritability estimates were larger when phenotyped using traditional methods relative to digital image analysis, suggesting that further refinements are required to better correlate digital image analysis with the traditional phenotyping methods.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"4 5","pages":"224-234"},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jsf2.192","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140702180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Total phenolic and flavonoid content of P. armata honey and propolis produced in Bomet, Kisii and Maralal, Kenya","authors":"Timothy Mugodo Kegode,&nbsp;Hosea O. Mokaya,&nbsp;Nkoba Kiatoko","doi":"10.1002/jsf2.190","DOIUrl":"https://doi.org/10.1002/jsf2.190","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Bee products are gaining interest in the field of research due to their biochemical and nutritive properties. Honey bee products have been researched extensively but little has been done in regards to stingless bees. There are many species of stingless bees including <i>Plebeina armata</i>, which are found in the Afrotropics. They are underground nesting and produce honey, propolis, wax, pollen and bee bread. These products are known to be rich in polyphenols that comprise of flavonoids and phenolic. In our study, we analysed colorimetrically the total flavonoid, phenolic content and radical scavenging activity of honey (<i>n</i> = 22) and propolis (<i>n</i> = 25) from Bomet, Kisii and Maralal in Kenya.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Honey and propolis had total flavonoid content of 12.00–22.67 mg QE/100 g and 288.15–944.76 mg QE/100 g while total phenolic content was 87.01–239.93 mg GAE/100 g and 524.14–1225.01 mg GAE/100 g, respectively. In considerations to the regions, Maralal had the highest phenolic and flavanoid content followed by Bomet and Kisii was the least. The same trend was observed in the radical scavenging activity. Except for the total flavonoid content in honey, the difference was significant (<i>p</i> &lt; 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The polyphenol content of both honey and propolis of <i>P. armata</i> are equally affected by geographical location as a result of different vegetation. They are good source of antioxidants, which can be utilized in diet due to their radical scavenging properties.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"4 4","pages":"191-196"},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jsf2.190","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140651223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of quinoa protein concentration and oil volume fraction on the physicochemical and mechanical properties of alginate-based emulsion gels","authors":"Nadia Lingiardi,&nbsp;Micaela Galante,&nbsp;Darío Spelzini","doi":"10.1002/jsf2.189","DOIUrl":"https://doi.org/10.1002/jsf2.189","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>This work studies emulsion gel (EG) formulation with quinoa proteins (QP), high-oleic sunflower oil and alginate, and the effect of QP concentration (0.5–1–2%) and the oil volume fraction (10–30–50%) on the physicochemical and mechanical properties of EG systems. EGs were tested for their microstructural and textural attributes, color, and water holding capacity as well as for their thermal, physical, and oxidative stability.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The microstructure of EG showed that with increasing QP concentrations, the gel structure tended to be much denser, with oil droplets entrapped within the network. A significant decrease in droplet diameter with increasing QP concentration (<i>p</i> = 0.015) and oil volume fraction (<i>p</i> &lt; 0.000) was observed. Hardness mean value was 2.8 N ± 0.5, reaching the highest value with 1 and 2% QP and 30% oil (<i>p</i> &lt; 0.000). Cohesiveness shows a similar trend to that observed for hardness, while springiness showed the opposite behavior. As for adhesiveness, there were no significant differences between samples. EG have high lightness with slight yellow and green contributions. The mean water holding capacity was 88 ± 4%, and after heat treatment all samples exhibited a good fluid retention, significantly lower for the lower oil volume fraction (<i>p</i> = 0.001). EG, also proved to be highly stable against creaming and oxidative damage.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Results suggest that EG could be useful to create a new generation of healthier and innovative products that could substitute animal fat and deliver nutrients and biological compounds, thus improving food quality.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"4 4","pages":"208-219"},"PeriodicalIF":0.0,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jsf2.189","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140651140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of pH and biopolymer ratio on phase behavior, rheology, and structural characteristics of pea protein isolate-locust bean gum coacervates","authors":"Mekala Pavani,&nbsp;Poonam Singha,&nbsp;Sushil Kumar Singh","doi":"10.1002/jsf2.187","DOIUrl":"10.1002/jsf2.187","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Interaction between plant-based proteins and polysaccharides depends on several factors.   This work reports the influence of pH and biopolymer ratios on the protein (pea protein isolate, PPI)-polysaccharide (locust bean gum, LBG) coacervates. Electrostatic interaction and hydrogen bond between the biopolymers strongly influenced the formation of the coacervates.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The optimum coacervate conditions were observed at pH 4.5 and PPI:LBG ratio of 5:1. The coacervates showed a honeycomb porous architecture having an amorphous nature. Complex coacervates showed a significant elevation of denaturation temperature as compared to biopolymers alone.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>These results indicates that PPI-LBG coacervates can be used as an effective biomaterial for encapsulating heat-sensitive bioactive compounds and other multiple uses in food processing.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"4 4","pages":"197-207"},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jsf2.187","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140384856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fully automatic quantitation of eight different metabolites in coffee using 1H-NMR spectroscopy and the PULCON methodology","authors":"Vera Gottstein,&nbsp;Dirk W. Lachenmeier,&nbsp;Thomas Kuballa,&nbsp;Mirko Bunzel","doi":"10.1002/jsf2.184","DOIUrl":"10.1002/jsf2.184","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Coffee contains a plethora of constituents with some of them being especially important either due to their physiological effects or as quality markers. As quantitative proton nuclear magnetic resonance spectroscopy (¹H-NMR) has been established as a fast and reliable analytical tool its application was evaluated for the simultaneous quantitation of lactic acid, acetic acid, formic acid, caffeine, caffeoylquinic acid (CQA) isomers, <i>N</i>-methylpyridinium, trigonelline, and 5-hydroxymethylfurfural (HMF) in aqueous extracts of roasted <i>Coffea arabica</i> samples.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Simultaneous quantitative determination was achieved by an automated analysis based on the PULCON methodology (pulse length-based concentration determination). The method was validated regarding linearity, accuracy, precision, limit of detection (LOD), and limit of quantitation (LOQ). Recovery rates were between 76% (CQA) and 116% (HMF), and precision was between 1.7% (caffeine) and 10.3% (HMF). The LOD varied between 0.06 g/kg (HMF) and 1.35 g/kg (caffeine and CQA), with the LOQ being between 0.22 g/kg (HMF) and 4.87 g/kg (CQA). To verify the results of the ¹H-NMR method, caffeine, trigonelline, HMF, 3-CQA, 4-CQA, and 5-CQA were additionally quantitated by HPLC-DAD and the results were compared. The described ¹H-NMR method was additionally applied to coffee samples that contained different coffee defects. Results showed only slight changes in the concentrations of the analytes by adding defective beans to defect-free coffee.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Discussion</h3>\u0000 \u0000 <p>The developed 1H-NMR approach was proven to be fast (30 min), reliable, and precise. Thus, it is well suited to analyze several coffee constituents of interest in a large number of samples in, for example, quality control.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"4 3","pages":"163-174"},"PeriodicalIF":0.0,"publicationDate":"2024-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jsf2.184","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140255188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}