Giuseppe Falcone, Mariangela Mazzone, Stefania Cesa, Francesco Cairone, Simone Carradori, Francesco Cellini, Annalisa Chiavaroli, Giustino Orlando, Maria Loreta Libero, Vittoria Perrotti, Maria Carmela Di Marcantonio, Gabriella Mincione
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This extract was characterized by chlorogenic acid and 12 glycosylated anthocyanins, being the most abundant delphinidin-3-O-galactoside, delphinidin-3-O-arabinoside, malvidin-3-O-galactoside, malvidin-3-O-glucoside, and malvidin-3-O-arabinoside. The blueberry extract (BL) was further used in extensive biological evaluation. Head and neck cell lines, namely CAL27 and A253, were treated at different concentrations in order to evaluate cell migration ability and ErbB receptors and antioxidant enzymes gene expression.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>The gene expression analysis highlighted the capability of BL to reduce ErbB receptors expression in CAL27 cell lines. The same treatments induced an opposite effect in A253 cell line for ErbB2/ErbB3 receptors. Moreover, in A253 cell line, BL increased SOD2 levels and reduced cell migration. The action of BL was also studied in an <i>ex vivo</i> experimental model of colon inflammation and was effective both in reducing the lipopolysaccharide-induced gene expression of different proinflammatory biomarkers involved in colon inflammation, among which tumor necrosis factor α, interleukin (IL)-6, and in stimulating the gene expression of the anti-inflammatory cytokine IL-10.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>The content of specific anthocyanins and chlorogenic acid can be considered responsible of such biological activities providing a new food supplement as coadjuvant of standard therapies.</p>\n </section>\n </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"5 5","pages":"199-211"},"PeriodicalIF":1.1000,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jsf2.230","citationCount":"0","resultStr":"{\"title\":\"The anthocyanin-enriched extract of Vaccinium corymbosum exerted in vitro and ex vivo antiproliferative and anti-inflammatory activities\",\"authors\":\"Giuseppe Falcone, Mariangela Mazzone, Stefania Cesa, Francesco Cairone, Simone Carradori, Francesco Cellini, Annalisa Chiavaroli, Giustino Orlando, Maria Loreta Libero, Vittoria Perrotti, Maria Carmela Di Marcantonio, Gabriella Mincione\",\"doi\":\"10.1002/jsf2.230\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p><i>Vaccinium corymbosum</i> berries represent a source of anthocyanins and polyphenols studied and tested for their healthy potential. The present study was aimed at isolating, characterizing, and quantifying the anthocyanin-enriched fraction obtained from blueberry and at assessing its biological and protective effects against head and neck cell lines and under inflammatory-related conditions. <i>V. corymbosum</i> berries extract was subjected to colorimetric analysis, antioxidant evaluation, and HPLC-DAD analysis. This extract was characterized by chlorogenic acid and 12 glycosylated anthocyanins, being the most abundant delphinidin-3-O-galactoside, delphinidin-3-O-arabinoside, malvidin-3-O-galactoside, malvidin-3-O-glucoside, and malvidin-3-O-arabinoside. The blueberry extract (BL) was further used in extensive biological evaluation. Head and neck cell lines, namely CAL27 and A253, were treated at different concentrations in order to evaluate cell migration ability and ErbB receptors and antioxidant enzymes gene expression.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>The gene expression analysis highlighted the capability of BL to reduce ErbB receptors expression in CAL27 cell lines. The same treatments induced an opposite effect in A253 cell line for ErbB2/ErbB3 receptors. Moreover, in A253 cell line, BL increased SOD2 levels and reduced cell migration. 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引用次数: 0
摘要
蓝莓是花青素和多酚的一种来源，对其健康潜力进行了研究和测试。本研究旨在分离、表征和定量从蓝莓中获得的富含花青素的部分，并评估其对头颈部细胞系和炎症相关条件下的生物学和保护作用。采用比色分析、抗氧化评价和HPLC-DAD分析。该提取物的主要成分为绿原酸和12种糖基化花青素，其中丰度最高的为3- o -半乳糖苷、3- o -阿拉伯糖苷、3- o -半乳糖苷、3- o -葡糖苷和3- o -阿拉伯糖苷。蓝莓提取物（BL）进一步广泛应用于生物学评价。采用不同浓度处理头颈部细胞系CAL27和A253，观察细胞迁移能力及ErbB受体和抗氧化酶基因表达。结果在CAL27细胞系中，基因表达分析显示了BL对ErbB受体表达的抑制作用。同样的处理在A253细胞系中对ErbB2/ErbB3受体产生相反的作用。此外，在A253细胞系中，BL增加了SOD2水平，减少了细胞迁移。在离体结肠炎症实验模型中研究了BL的作用，发现它既能降低脂多糖诱导的结肠炎症相关的不同促炎生物标志物（如肿瘤坏死因子α、白细胞介素(IL)-6）的基因表达，又能刺激抗炎细胞因子IL-10的基因表达。结论特定花青素和绿原酸的含量可被认为与这些生物活性有关，为标准治疗提供了一种新的辅助食品补充剂。
The anthocyanin-enriched extract of Vaccinium corymbosum exerted in vitro and ex vivo antiproliferative and anti-inflammatory activities
Background
Vaccinium corymbosum berries represent a source of anthocyanins and polyphenols studied and tested for their healthy potential. The present study was aimed at isolating, characterizing, and quantifying the anthocyanin-enriched fraction obtained from blueberry and at assessing its biological and protective effects against head and neck cell lines and under inflammatory-related conditions. V. corymbosum berries extract was subjected to colorimetric analysis, antioxidant evaluation, and HPLC-DAD analysis. This extract was characterized by chlorogenic acid and 12 glycosylated anthocyanins, being the most abundant delphinidin-3-O-galactoside, delphinidin-3-O-arabinoside, malvidin-3-O-galactoside, malvidin-3-O-glucoside, and malvidin-3-O-arabinoside. The blueberry extract (BL) was further used in extensive biological evaluation. Head and neck cell lines, namely CAL27 and A253, were treated at different concentrations in order to evaluate cell migration ability and ErbB receptors and antioxidant enzymes gene expression.
Results
The gene expression analysis highlighted the capability of BL to reduce ErbB receptors expression in CAL27 cell lines. The same treatments induced an opposite effect in A253 cell line for ErbB2/ErbB3 receptors. Moreover, in A253 cell line, BL increased SOD2 levels and reduced cell migration. The action of BL was also studied in an ex vivo experimental model of colon inflammation and was effective both in reducing the lipopolysaccharide-induced gene expression of different proinflammatory biomarkers involved in colon inflammation, among which tumor necrosis factor α, interleukin (IL)-6, and in stimulating the gene expression of the anti-inflammatory cytokine IL-10.
Conclusion
The content of specific anthocyanins and chlorogenic acid can be considered responsible of such biological activities providing a new food supplement as coadjuvant of standard therapies.
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