Biologics (Basel, Switzerland)最新文献_第6页

Optimization of a New Antihyperglycemic Formulation Using a Mixture of Linum usitatissimum L., Coriandrum sativum L., and Olea europaea var. sylvestris Flavonoids: A Mixture Design Approach 以亚麻、五味子、木犀总黄酮为主要成分的降血糖新配方的优化设计

Biologics (Basel, Switzerland) Pub Date : 2021-07-21 DOI: 10.3390/BIOLOGICS1020009

Hamza Mechchate, Wessal Ouedrhiri, I. Es-safi, Amal Amaghnouje, F. Jawhari, D. Bousta

{"title":"Optimization of a New Antihyperglycemic Formulation Using a Mixture of Linum usitatissimum L., Coriandrum sativum L., and Olea europaea var. sylvestris Flavonoids: A Mixture Design Approach","authors":"Hamza Mechchate, Wessal Ouedrhiri, I. Es-safi, Amal Amaghnouje, F. Jawhari, D. Bousta","doi":"10.3390/BIOLOGICS1020009","DOIUrl":"https://doi.org/10.3390/BIOLOGICS1020009","url":null,"abstract":"Flavonoids are a class of natural chemicals with variable phenolic structures that have long been recognized for their health advantages, they have recently attracted researchers’ attention for treating diabetes and hyperglycemia. The goal of this research is to develop a novel antihyperglycemic formulation using a combination of three plant flavonoids: Linum usitatissimum L. seeds (FLU), Coriandrum sativum L. seeds (FCS), and Olea europaea var. sylvestris leaves (FOE) based on a mixture design experiment approach which generates the most effective ratio of each component in a mixture instead of the trial-and-error method. Prior to the test, sub-acute toxicity research was conducted to establish a safe and effective dosage. The Oral Glucose Tolerance Test (OGTT) was used to assess the antihyperglycemic impact of these extracts and their combinations in Swiss albino mice. The dose that showed efficacy and safety was 25 mg/kg, which was utilized in all formulations. According to the results, the binary and ternary combinations showed the most significant synergetic effects. The optimum combination with the most potent effect was 37% FLU, 20% FCS, and 43% FOE. This study’s mixture design and prediction model for glycemic variation (GV) may be utilized at an industrial level to develop a novel antidiabetic and antihyperglycemic formulation that is safe and effective.","PeriodicalId":93526,"journal":{"name":"Biologics (Basel, Switzerland)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3390/BIOLOGICS1020009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42766522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Induction of the CD24 Surface Antigen in Primary Undifferentiated Human Adipose Progenitor Cells by the Hedgehog Signaling Pathway Hedgehog信号通路诱导CD24表面抗原在原代未分化人脂肪祖细胞中的作用

Biologics (Basel, Switzerland) Pub Date : 2021-07-21 DOI: 10.3390/BIOLOGICS1020008

F. Muoio, S. Panella, Yves Harder, T. Tallone

{"title":"Induction of the CD24 Surface Antigen in Primary Undifferentiated Human Adipose Progenitor Cells by the Hedgehog Signaling Pathway","authors":"F. Muoio, S. Panella, Yves Harder, T. Tallone","doi":"10.3390/BIOLOGICS1020008","DOIUrl":"https://doi.org/10.3390/BIOLOGICS1020008","url":null,"abstract":"In the murine model system of adipogenesis, the CD24 cell surface protein represents a valuable marker to label undifferentiated adipose progenitor cells. Indeed, when injected into the residual fat pads of lipodystrophic mice, these CD24 positive cells reconstitute a normal white adipose tissue (WAT) depot. Unluckily, similar studies in humans are rare and incomplete. This is because it is impossible to obtain large numbers of primary CD24 positive human adipose stem cells (hASCs). This study shows that primary hASCs start to express the glycosylphosphatidylinositol (GPI)-anchored CD24 protein when cultured with a chemically defined medium supplemented with molecules that activate the Hedgehog (Hh) signaling pathway. Therefore, this in vitro system may help understand the biology and role in adipogenesis of the CD24-positive hASCs. The induced cells’ phenotype was studied by flow cytometry, Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) techniques, and their secretion profile. The results show that CD24 positive cells are early undifferentiated progenitors expressing molecules related to the angiogenic pathway.","PeriodicalId":93526,"journal":{"name":"Biologics (Basel, Switzerland)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3390/BIOLOGICS1020008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44002086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational Chemistry to Repurposing Drugs for the Control of COVID-19 计算化学重新利用药物控制新冠肺炎

Biologics (Basel, Switzerland) Pub Date : 2021-07-15 DOI: 10.3390/biologics1020007

Majid Hassanzadeganroudsari, Amirmasoud Ahmadi, Niloufar Rashidi, Md Kamal Hossain, Amanda Habib, V. Apostolopoulos

{"title":"Computational Chemistry to Repurposing Drugs for the Control of COVID-19","authors":"Majid Hassanzadeganroudsari, Amirmasoud Ahmadi, Niloufar Rashidi, Md Kamal Hossain, Amanda Habib, V. Apostolopoulos","doi":"10.3390/biologics1020007","DOIUrl":"https://doi.org/10.3390/biologics1020007","url":null,"abstract":"Thus far, in 2021, 219 countries with over 175 million people have been infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). SARS-CoV-2 is a positive sense, single-stranded RNA virus, and is the causal agent for coronavirus disease (COVID-19). Due to the urgency of the situation, virtual screening as a computational modeling method offers a fast and effective modality of identifying drugs that may be effective against SARS-CoV-2. There has been an overwhelming abundance of molecular docking against SARS-CoV-2 in the last year. Due to the massive volume of computational studies, this systematic review has been created to evaluate and summarize the findings of existing studies. Herein, we report on computational articles of drugs which target, (1) viral protease, (2) Spike protein-ACE 2 interaction, (3) RNA-dependent RNA polymerase, and (4) other proteins and nonstructural proteins of SARS-CoV-2. Based on the studies presented, there are 55 identified natural or drug compounds with potential anti-viral activity. The next step is to show anti-viral activity in vitro and translation to determine effectiveness into human clinical trials.","PeriodicalId":93526,"journal":{"name":"Biologics (Basel, Switzerland)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3390/biologics1020007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42131078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Involvement of Probiotics and Postbiotics in the Immune System Modulation 益生菌和后益生菌在免疫系统调节中的作用

Biologics (Basel, Switzerland) Pub Date : 2021-07-06 DOI: 10.3390/BIOLOGICS1020006

Neslihan Yeşilyurt, Birsen Yılmaz, D. Ağagündüz, R. Capasso

{"title":"Involvement of Probiotics and Postbiotics in the Immune System Modulation","authors":"Neslihan Yeşilyurt, Birsen Yılmaz, D. Ağagündüz, R. Capasso","doi":"10.3390/BIOLOGICS1020006","DOIUrl":"https://doi.org/10.3390/BIOLOGICS1020006","url":null,"abstract":"Intestinal microbiota interacts with other systems, especially the immune system, which is responsible for protecting the body by recognizing “stranger” (pathogen associated molecular patterns-PAMPs) and “danger” (damage-associated molecular patterns-DAMPs) molecular motifs. In this manner, it plays an important role in the pathogenesis of various diseases and health. Despite the use of probiotics that modulate the intestinal microbiota in providing health benefits and in the treatment of diseases, there are some possible concerns about the possibility of developing adverse effects, especially in people with suppressed immune systems. Since probiotics provide health benefits with bioactive compounds, studies are carried out on the use of products containing non-living probiotic microorganisms (paraprobiotics) and/or their metabolites (postbiotics) instead of probiotic products. It is even reported that these microbial compounds have more immunomodulatory activities than living microorganisms via some possible mechanism and eliminates some disadvantages of probiotics. Considering the increasing use of functional foods in health and disease, further studies are needed with respect to the benefits and advantages of parabiotic and/or postbiotic use in the food and pharmaceutical industry as well as immune system modulation. Although probiotics have been extensive studied for a long time, it seems that postbiotics are promising tools for future research and applications according to the recent literature. This review aimed to evaluate the interaction of probiotics and postbiotics with the immune systems and also their advantages and disadvantages in the area of food-pharmaceutical industry and immune system modulation.","PeriodicalId":93526,"journal":{"name":"Biologics (Basel, Switzerland)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3390/BIOLOGICS1020006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46946996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 54

How to Train Your Phage: The Recent Efforts in Phage Training 如何训练你的噬菌体:噬菌体训练的最新进展

Biologics (Basel, Switzerland) Pub Date : 2021-07-05 DOI: 10.3390/BIOLOGICS1020005

Abdallah S. Abdelsattar, Alyaa Dawooud, Nouran Rezk, Salsabil Makky, Anan Safwat, P. Richards, A. El-Shibiny

{"title":"How to Train Your Phage: The Recent Efforts in Phage Training","authors":"Abdallah S. Abdelsattar, Alyaa Dawooud, Nouran Rezk, Salsabil Makky, Anan Safwat, P. Richards, A. El-Shibiny","doi":"10.3390/BIOLOGICS1020005","DOIUrl":"https://doi.org/10.3390/BIOLOGICS1020005","url":null,"abstract":"Control of pathogenic bacteria by deliberate application of predatory phages has potential as a powerful therapy against antibiotic-resistant bacteria. The key advantages of phage biocontrol over antibacterial chemotherapy are: (1) an ability to self-propagate inside host bacteria, (2) targeted predation of specific species or strains of bacteria, (3) adaptive molecular machinery to overcome resistance in target bacteria. However, realizing the potential of phage biocontrol is dependent on harnessing or adapting these responses, as many phage species switch between lytic infection cycles (resulting in lysis) and lysogenic infection cycles (resulting in genomic integration) that increase the likelihood of survival of the phage in response to external stress or host depletion. Similarly, host range will need to be optimized to make phage therapy medically viable whilst avoiding the potential for deleteriously disturbing the commensal microbiota. Phage training is a new approach to produce efficient phages by capitalizing on the evolved response of wild-type phages to bacterial resistance. Here we will review recent studies reporting successful trials of training different strains of phages to switch into lytic replication mode, overcome bacterial resistance, and increase their host range. This review will also highlight the current knowledge of phage training and future implications in phage applications and phage therapy and summarize the recent pipeline of the magistral preparation to produce a customized phage for clinical trials and medical applications.","PeriodicalId":93526,"journal":{"name":"Biologics (Basel, Switzerland)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3390/BIOLOGICS1020005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41603582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Can the European Hedgehog (Erinaceus europaeus) Be a Sentinel for One Health Concerns? 欧洲刺猬(Erinaceus europaeus)能否成为一种健康问题的哨兵?

Biologics (Basel, Switzerland) Pub Date : 2021-06-17 DOI: 10.3390/BIOLOGICS1010004

C. Jota Baptista, F. Seixas, J. M. Gonzalo-Orden, P. Oliveira

{"title":"Can the European Hedgehog (Erinaceus europaeus) Be a Sentinel for One Health Concerns?","authors":"C. Jota Baptista, F. Seixas, J. M. Gonzalo-Orden, P. Oliveira","doi":"10.3390/BIOLOGICS1010004","DOIUrl":"https://doi.org/10.3390/BIOLOGICS1010004","url":null,"abstract":"Erinaceus europaeus is a cosmopolitan mammalian species broadly distributed in Europe, from natural to suburban areas. Due to its ecological role and susceptibility to distinct zoonotic agents, E. europaeus could be a suitable sentinel candidate for many global problems that negatively affect human and animal health. Hedgehogs can work as bioindicators to environmental contamination and can be hosts for multiple tickborne zoonotic agents. Thus, people who directly or indirectly make physical contact with this species are exposed to a variety of threats. Moreover, it has also been studied as an indicator for antibiotic resistance, which was already confirmed for tetracyclines. Additionally, it was also reported as a reservoir for methicillin-resistant Staphylococcus aureus (MRSA). More recently, hedgehogs have been recently recognised as potential reservoirs of MERS-CoV-like strains. Among other animals, this species can possibly represent an intermediate reservoir for SARS-CoV-2. The aim of this review is to briefly expose the scientific attainments about hedgehog health, namely agents, diseases, and threats that significantly affect general health concerns and that contribute to achieve One Health principles.","PeriodicalId":93526,"journal":{"name":"Biologics (Basel, Switzerland)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3390/BIOLOGICS1010004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44593098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

The Peptide TAT-I24 with Antiviral Activity against DNA Viruses Binds Double-Stranded DNA with High Affinity 具有抗DNA病毒活性的肽TAT-I24与高亲和力双链DNA结合

Biologics (Basel, Switzerland) Pub Date : 2021-06-10 DOI: 10.3390/BIOLOGICS1010003

H. Harant, S. Höfinger, F. Kricek, C. Ruf, Z. Ruzsics, H. Hengel, I. Lindley

{"title":"The Peptide TAT-I24 with Antiviral Activity against DNA Viruses Binds Double-Stranded DNA with High Affinity","authors":"H. Harant, S. Höfinger, F. Kricek, C. Ruf, Z. Ruzsics, H. Hengel, I. Lindley","doi":"10.3390/BIOLOGICS1010003","DOIUrl":"https://doi.org/10.3390/BIOLOGICS1010003","url":null,"abstract":"The peptide TAT-I24, composed of the 9-mer peptide I24 and the TAT (48-60) peptide, exerts broad-spectrum antiviral activity against several DNA viruses. The current model of the mode of action suggests a reduction of viral entry and also a possible interaction with the viral DNA upon virus entry. To further support this model, the present study investigates the DNA binding properties of TAT-I24. DNA binding was analysed by gel retardation of a peptide-complexed DNA, fluorescence reduction of DNA labelled with intercalating dyes and determination of binding kinetics by surface plasmon resonance. Molecular dynamics simulations of DNA-peptide complexes predict high-affinity binding and destabilization of the DNA by TAT-I24. The effect on viral DNA levels of infected cells were studied by real-time PCR and staining of viral DNA by bromodeoxyuridine. TAT-I24 binds double-stranded DNA with high affinity, leading to inhibition of polymerase binding and thereby blocking of de novo nucleic acid synthesis. Analysis of early steps of virus entry using a bromodeoxyuridine-labelled virus as well as quantification of viral genomes in the cells indicate direct binding of the peptide to the viral DNA. Saturation of the peptide with exogenous DNA can fully neutralize the inhibitory effect. The antiviral activity of TAT-I24 is linked to its ability to bind DNA with high affinity. This mechanism could be the basis for the development of novel antiviral agents.","PeriodicalId":93526,"journal":{"name":"Biologics (Basel, Switzerland)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3390/BIOLOGICS1010003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48005393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Overview of COVID-19 Disease: Virology, Epidemiology, Prevention Diagnosis, Treatment, and Vaccines COVID-19疾病概述:病毒学、流行病学、预防、诊断、治疗和疫苗

Biologics (Basel, Switzerland) Pub Date : 2021-05-12 DOI: 10.3390/BIOLOGICS1010002

Iman Salahshoori, Noushin Mobaraki-asl, A. Seyfaee, Nasrin Mirzaei Nasirabad, Z. Dehghan, Mehrdad Faraji, Mina Ganjkhani, A. Babapoor, Seyede Zahra Shadmehr, A. Hamrang

{"title":"Overview of COVID-19 Disease: Virology, Epidemiology, Prevention Diagnosis, Treatment, and Vaccines","authors":"Iman Salahshoori, Noushin Mobaraki-asl, A. Seyfaee, Nasrin Mirzaei Nasirabad, Z. Dehghan, Mehrdad Faraji, Mina Ganjkhani, A. Babapoor, Seyede Zahra Shadmehr, A. Hamrang","doi":"10.3390/BIOLOGICS1010002","DOIUrl":"https://doi.org/10.3390/BIOLOGICS1010002","url":null,"abstract":"Coronaviruses belong to the “Coronaviridae family”, which causes various diseases, from the common cold to SARS and MERS. The coronavirus is naturally prevalent in mammals and birds. So far, six human-transmitted coronaviruses have been discovered. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first reported in December 2019 in Wuhan, China. Common symptoms include fever, dry cough, and fatigue, but in acute cases, the disease can lead to severe shortness of breath, hypoxia, and death. According to the World Health Organization (WHO), the three main transmission routes, such as droplet and contact routes, airborne transmission and fecal and oral for COVID-19, have been identified. So far, no definitive curative treatment has been discovered for COVID-19, and the available treatments are only to reduce the complications of the disease. According to the World Health Organization, preventive measures at the public health level such as quarantine of the infected person, identification and monitoring of contacts, disinfection of the environment, and personal protective equipment can significantly prevent the outbreak COVID-19. Currently, based on the urgent needs of the community to control this pandemic, the BNT162b2 (Pfizer), mRNA-1273 (Moderna), CoronaVac (Sinovac), Sputnik V (Gamaleya Research Institute, Acellena Contract Drug Research, and Development), BBIBP-CorV (Sinofarm), and AZD1222 (The University of Oxford; AstraZeneca) vaccines have received emergency vaccination licenses from health organizations in vaccine-producing countries. Vasso Apostolopoulos, Majid Hassanzadeganroudsari","PeriodicalId":93526,"journal":{"name":"Biologics (Basel, Switzerland)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3390/BIOLOGICS1010002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45765975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18