PhotoniX最新文献_第4页

Visualizing cortical blood perfusion after photothrombotic stroke in vivo by needle-shaped beam optical coherence tomography angiography 通过针形光束光学相干断层血管成像技术观察光血栓性中风后体内皮层血液灌注情况

PhotoniX Pub Date : 2024-03-27 DOI: 10.1186/s43074-024-00124-9

Xiangyu Guo, Jingjing Zhao, Liqun Sun, Varun Gupta, Lin Du, Komal Sharma, Aidan Van Vleck, Kaitlyn Liang, Liangcai Cao, Lingjie Kong, Yuanmu Yang, Yong Huang, Adam de la Zerda, Guofan Jin

{"title":"Visualizing cortical blood perfusion after photothrombotic stroke in vivo by needle-shaped beam optical coherence tomography angiography","authors":"Xiangyu Guo, Jingjing Zhao, Liqun Sun, Varun Gupta, Lin Du, Komal Sharma, Aidan Van Vleck, Kaitlyn Liang, Liangcai Cao, Lingjie Kong, Yuanmu Yang, Yong Huang, Adam de la Zerda, Guofan Jin","doi":"10.1186/s43074-024-00124-9","DOIUrl":"https://doi.org/10.1186/s43074-024-00124-9","url":null,"abstract":"Optical imaging techniques provide low-cost, non-radiative images with high spatiotemporal resolution, making them advantageous for long-term dynamic observation of blood perfusion in stroke research and other brain studies compared to non-optical methods. However, high-resolution imaging in optical microscopy fundamentally requires a tight optical focus, and thus a limited depth of field (DOF). Consequently, large-scale, non-stitched, high-resolution images of curved surfaces, like brains, are difficult to acquire without z-axis scanning. To overcome this limitation, we developed a needle-shaped beam optical coherence tomography angiography (NB-OCTA) system, and for the first time, achieved a volumetric resolution of less than 8 μm in a non-stitched volume space of 6.4 mm × 4 mm × 620 μm in vivo. This system captures the distribution of blood vessels at 3.4-times larger depths than normal OCTA equipped with a Gaussian beam (GB-OCTA). We then employed NB-OCTA to perform long-term observation of cortical blood perfusion after stroke in vivo, and quantitatively analyzed the vessel area density (VAD) and the diameters of representative vessels in different regions over 10 days, revealing different spatiotemporal dynamics in the acute, sub-acute and chronic phase of post-ischemic revascularization. Benefiting from our NB-OCTA, we revealed that the recovery process is not only the result of spontaneous reperfusion, but also the formation of new vessels. This study provides visual and mechanistic insights into strokes and helps to deepen our understanding of the spontaneous response of brain after stroke.","PeriodicalId":93483,"journal":{"name":"PhotoniX","volume":"235 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140314275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrafast laser one-step construction of 3D micro-/nanostructures achieving high-performance zinc metal anodes 超快激光一步法构建三维微/纳米结构，实现高性能锌金属阳极

PhotoniX Pub Date : 2024-03-19 DOI: 10.1186/s43074-024-00122-x

Yanan Liu, Ye Ding, Zeping Liu, Xingchen Li, Sichao Tian, Lishuang Fan, Jichang Xie, Liangliang Xu, Jinwoo Lee, Jian Li, Lijun Yang

{"title":"Ultrafast laser one-step construction of 3D micro-/nanostructures achieving high-performance zinc metal anodes","authors":"Yanan Liu, Ye Ding, Zeping Liu, Xingchen Li, Sichao Tian, Lishuang Fan, Jichang Xie, Liangliang Xu, Jinwoo Lee, Jian Li, Lijun Yang","doi":"10.1186/s43074-024-00122-x","DOIUrl":"https://doi.org/10.1186/s43074-024-00122-x","url":null,"abstract":"Aqueous zinc-ion batteries provide a most promising alternative to the existing lithium-ion batteries due to their high theoretical capacity, intrinsic safety, and low cost. However, commercializing aqueous zinc-ion batteries suffer from dendritic growth and side reactions on the surface of metallic zinc, resulting in poor reversibility. To overcome this critical challenge, here, we report a one-step ultrafast laser processing method for fabricating three-dimensional micro-/nanostructures on zinc anodes to optimize zinc nucleation and deposition processes. It is demonstrated that the three-dimensional micro-/nanostructure with increased specific surface area significantly reduces nucleation overpotential, as well as preferentially absorbs zinc ions to prevent dendritic protuberances and corrosion. As a result, the presence of three-dimensional micro-/nanostructures on the zinc metal delivers stable zinc plating/stripping beyond 2500 h (2 mA cm-2/1 mAh cm-2) in symmetric cells, a high Coulombic efficiency (99.71%) in half cells, and moreover an improved capacity retention (71.8%) is also observed in full cells. Equally intriguingly, the pouch cell with three-dimensional micro-/nanostructures can operate across various bending states without severely compromising performance. This work provides an effective strategy to construct ultrafine and high-precision three-dimensional micro-/nanostructures achieving high-performance zinc metal anodes and is expected to be of immediate benefit to other metal-based electrodes.","PeriodicalId":93483,"journal":{"name":"PhotoniX","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140171802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vacuum-ultraviolet (λ < 200 nm) photodetector array 真空-紫外线（λ < 200 纳米）光电探测器阵列

PhotoniX Pub Date : 2024-03-13 DOI: 10.1186/s43074-024-00120-z

Siqi Zhu, Zhuogeng Lin, Zhao Wang, Lemin Jia, Naiji Zhang, Wei Zheng

引用次数: 0

Self-supervised denoising for multimodal structured illumination microscopy enables long-term super-resolution live-cell imaging 用于多模态结构照明显微镜的自监督去噪技术可实现长期超分辨率活细胞成像

PhotoniX Pub Date : 2024-03-01 DOI: 10.1186/s43074-024-00121-y

Xingye Chen, Chang Qiao, Tao Jiang, Jiahao Liu, Quan Meng, Yunmin Zeng, Haoyu Chen, Hui Qiao, Dong Li, Jiamin Wu

{"title":"Self-supervised denoising for multimodal structured illumination microscopy enables long-term super-resolution live-cell imaging","authors":"Xingye Chen, Chang Qiao, Tao Jiang, Jiahao Liu, Quan Meng, Yunmin Zeng, Haoyu Chen, Hui Qiao, Dong Li, Jiamin Wu","doi":"10.1186/s43074-024-00121-y","DOIUrl":"https://doi.org/10.1186/s43074-024-00121-y","url":null,"abstract":"Detection noise significantly degrades the quality of structured illumination microscopy (SIM) images, especially under low-light conditions. Although supervised learning based denoising methods have shown prominent advances in eliminating the noise-induced artifacts, the requirement of a large amount of high-quality training data severely limits their applications. Here we developed a pixel-realignment-based self-supervised denoising framework for SIM (PRS-SIM) that trains an SIM image denoiser with only noisy data and substantially removes the reconstruction artifacts. We demonstrated that PRS-SIM generates artifact-free images with 20-fold less fluorescence than ordinary imaging conditions while achieving comparable super-resolution capability to the ground truth (GT). Moreover, we developed an easy-to-use plugin that enables both training and implementation of PRS-SIM for multimodal SIM platforms including 2D/3D and linear/nonlinear SIM. With PRS-SIM, we achieved long-term super-resolution live-cell imaging of various vulnerable bioprocesses, revealing the clustered distribution of Clathrin-coated pits and detailed interaction dynamics of multiple organelles and the cytoskeleton.","PeriodicalId":93483,"journal":{"name":"PhotoniX","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140008142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Observation of single-molecule Raman spectroscopy enabled by synergic electromagnetic and chemical enhancement 利用电磁和化学协同增强技术观测单分子拉曼光谱

PhotoniX Pub Date : 2024-02-29 DOI: 10.1186/s43074-024-00119-6

Haiyao Yang, Haoran Mo, Jianzhi Zhang, Lihong Hong, Zhi-Yuan Li

{"title":"Observation of single-molecule Raman spectroscopy enabled by synergic electromagnetic and chemical enhancement","authors":"Haiyao Yang, Haoran Mo, Jianzhi Zhang, Lihong Hong, Zhi-Yuan Li","doi":"10.1186/s43074-024-00119-6","DOIUrl":"https://doi.org/10.1186/s43074-024-00119-6","url":null,"abstract":"There has been a long fundamental pursuit to enhance and levitate the Raman scattering signal intensity of molecule by a huge number of ~ 14–15 orders of magnitude, to the level comparable with the molecule fluorescence intensity and truly entering the regime of single-molecule Raman spectroscopy. In this work we report unambiguous observation of single-molecule Raman spectroscopy via synergic action of electromagnetic and chemical enhancement for rhodamine B (RhB) molecule absorbed within the plasmonic nanogap formed by gold nanoparticle sitting on the two-dimensional (2D) monolayer WS2 and 2 nm SiO2 coated gold thin film. Raman spectroscopy down to an extremely dilute value of 10–18 mol/L can still be clearly visible, and the statistical enhancement factor could reach 16 orders of magnitude compared with the reference detection sample of silicon plate. The electromagnetic enhancement comes from local surface plasmon resonance induced at the nanogap, which could reach ~ 10–11 orders of magnitude, while the chemical enhancement comes from monolayer WS2 2D material, which could reach 4–5 orders of magnitudes. This synergic route of Raman enhancement devices could open up a new frontier of single molecule science, allowing detection, identification, and monitor of single molecules and their spatial–temporal evolution under various internal and external stimuli.","PeriodicalId":93483,"journal":{"name":"PhotoniX","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140008141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reconfigurable flexible metasurfaces: from fundamentals towards biomedical applications 可重新配置的柔性超表面：从基本原理到生物医学应用

PhotoniX Pub Date : 2024-01-22 DOI: 10.1186/s43074-023-00116-1

Jiangtao Tian, Wenhan Cao

引用次数: 0

Surmounting photon limits and motion artifacts for biological dynamics imaging via dual-perspective self-supervised learning 通过双视角自监督学习克服生物动态成像的光子限制和运动伪影

PhotoniX Pub Date : 2024-01-05 DOI: 10.1186/s43074-023-00117-0

Binglin Shen, Chenggui Luo, Wen Pang, Yajing Jiang, Wenbo Wu, Rui Hu, Junle Qu, Bobo Gu, Liwei Liu

{"title":"Surmounting photon limits and motion artifacts for biological dynamics imaging via dual-perspective self-supervised learning","authors":"Binglin Shen, Chenggui Luo, Wen Pang, Yajing Jiang, Wenbo Wu, Rui Hu, Junle Qu, Bobo Gu, Liwei Liu","doi":"10.1186/s43074-023-00117-0","DOIUrl":"https://doi.org/10.1186/s43074-023-00117-0","url":null,"abstract":"Visualizing rapid biological dynamics like neuronal signaling and microvascular flow is crucial yet challenging due to photon noise and motion artifacts. Here we present a deep learning framework for enhancing the spatiotemporal relations of optical microscopy data. Our approach leverages correlations of mirrored perspectives from conjugated scan paths, training a model to suppress noise and motion blur by restoring degraded spatial features. Quantitative validation on vibrational calcium imaging validates significant gains in spatiotemporal correlation (2.2×), signal-to-noise ratio (9–12 dB), structural similarity (6.6×), and motion tolerance compared to raw data. We further apply the framework to diverse in vivo experiments from mouse cerebral hemodynamics to zebrafish cardiac dynamics. This approach enables the clear visualization of the rapid nutrient flow (30 mm/s) in microcirculation and the systolic and diastolic processes of heartbeat (2.7 cycle/s), as well as cellular and vascular structure in deep cortex. Unlike techniques relying on temporal correlations, learning inherent spatial priors avoids motion-induced artifacts. This self-supervised strategy flexibly enhances live microscopy under photon-limited and motion-prone regimes.","PeriodicalId":93483,"journal":{"name":"PhotoniX","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139102121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep learning enhanced quantum holography with undetected photons. 深度学习增强了未探测光子的量子全息。

IF 15.7

PhotoniX Pub Date : 2024-01-01 Epub Date: 2024-12-18 DOI: 10.1186/s43074-024-00155-2

Weiru Fan, Gewei Qian, Yutong Wang, Chen-Ran Xu, Ziyang Chen, Xun Liu, Wei Li, Xu Liu, Feng Liu, Xingqi Xu, Da-Wei Wang, Vladislav V Yakovlev

{"title":"Deep learning enhanced quantum holography with undetected photons.","authors":"Weiru Fan, Gewei Qian, Yutong Wang, Chen-Ran Xu, Ziyang Chen, Xun Liu, Wei Li, Xu Liu, Feng Liu, Xingqi Xu, Da-Wei Wang, Vladislav V Yakovlev","doi":"10.1186/s43074-024-00155-2","DOIUrl":"10.1186/s43074-024-00155-2","url":null,"abstract":"Holography is an essential technique of generating three-dimensional images. Recently, quantum holography with undetected photons (QHUP) has emerged as a groundbreaking method capable of capturing complex amplitude images. Despite its potential, the practical application of QHUP has been limited by susceptibility to phase disturbances, low interference visibility, and limited spatial resolution. Deep learning, recognized for its ability in processing complex data, holds significant promise in addressing these challenges. In this report, we present an ample advancement in QHUP achieved by harnessing the power of deep learning to extract images from single-shot holograms, resulting in vastly reduced noise and distortion, alongside a notable enhancement in spatial resolution. The proposed and demonstrated deep learning QHUP (DL-QHUP) methodology offers a transformative solution by delivering high-speed imaging, improved spatial resolution, and superior noise resilience, making it suitable for diverse applications across an array of research fields stretching from biomedical imaging to remote sensing. DL-QHUP signifies a crucial leap forward in the realm of holography, demonstrating its immense potential to revolutionize imaging capabilities and pave the way for advancements in various scientific disciplines. The integration of DL-QHUP promises to unlock new possibilities in imaging applications, transcending existing limitations and offering unparalleled performance in challenging environments.Supplementary information: The online version contains supplementary material available at 10.1186/s43074-024-00155-2.","PeriodicalId":93483,"journal":{"name":"PhotoniX","volume":"5 1","pages":"40"},"PeriodicalIF":15.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11655614/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep tissue super-resolution imaging with adaptive optical two-photon multifocal structured illumination microscopy 利用自适应光学双光子多焦结构照明显微镜进行深层组织超分辨率成像

PhotoniX Pub Date : 2023-12-21 DOI: 10.1186/s43074-023-00115-2

Chenshuang Zhang, Bin Yu, Fangrui Lin, Soham Samanta, Huanhuan Yu, Wei Zhang, Yingying Jing, Chunfeng Shang, Danying Lin, Ke Si, Wei Gong, Junle Qu

{"title":"Deep tissue super-resolution imaging with adaptive optical two-photon multifocal structured illumination microscopy","authors":"Chenshuang Zhang, Bin Yu, Fangrui Lin, Soham Samanta, Huanhuan Yu, Wei Zhang, Yingying Jing, Chunfeng Shang, Danying Lin, Ke Si, Wei Gong, Junle Qu","doi":"10.1186/s43074-023-00115-2","DOIUrl":"https://doi.org/10.1186/s43074-023-00115-2","url":null,"abstract":"Visualization of axons and dendritic spines is crucial in neuroscience research. However, traditional microscopy is limited by diffraction-limited resolution and shallow imaging depth, making it difficult to study neuronal dynamics. Two-photon multifocal structured illumination microscopy (2P-MSIM) provides super-resolution imaging along with a reasonably good penetration, but it is vulnerable to optical aberrations in deep tissues. Herein we present a novel non-inertial scanning 2P-MSIM system incorporated with adaptive optics (AO) which allows for super-resolution imaging with effective aberration correction. Our strategy is designed to correct both laser and fluorescence paths simultaneously using a spatial light modulator and a deformable mirror respectively, providing better results than the individual path corrections. The successful implementation of adaptive optical two-photon multifocal structured illumination microscopy (AO 2P-MSIM) has allowed for the super-resolution imaging of neuronal structures in a mouse brain slice at great depths and dynamic morphological characteristics of zebrafish motoneurons in vivo.","PeriodicalId":93483,"journal":{"name":"PhotoniX","volume":"249 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138826048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chip-to-chip optical multimode communication with universal mode processors 用通用模式处理器的片对片光多模通信

PhotoniX Pub Date : 2023-10-27 DOI: 10.1186/s43074-023-00114-3

Bo Wu, Wenkai Zhang, Hailong Zhou, Jianji Dong, Dongmei Huang, P. K. A. Wai, Xinliang Zhang

{"title":"Chip-to-chip optical multimode communication with universal mode processors","authors":"Bo Wu, Wenkai Zhang, Hailong Zhou, Jianji Dong, Dongmei Huang, P. K. A. Wai, Xinliang Zhang","doi":"10.1186/s43074-023-00114-3","DOIUrl":"https://doi.org/10.1186/s43074-023-00114-3","url":null,"abstract":"Abstract The increasing amount of data exchange requires higher-capacity optical communication links. Mode division multiplexing (MDM) is considered as a promising technology to support the higher data throughput. In an MDM system, the mode generator and sorter are the backbone. However, most of the current schemes lack the programmability and universality, which makes the MDM link susceptible to the mode crosstalk and environmental disturbances. In this paper, we propose an intelligent multimode optical communication link using universal mode processing (generation and sorting) chips. The mode processor consists of a programmable 4 × 4 Mach Zehnder interferometer (MZI) network and can be intelligently configured to generate or sort both quasi linearly polarized (LP) modes and orbital angular momentum (OAM) modes in any desired routing state. We experimentally establish a chip-to-chip MDM communication system. The mode basis can be freely switched between four LP modes and four OAM modes. We also demonstrate the multimode optical communication capability at a data rate of 25 Gbit/s. The proposed scheme shows significant advantages in terms of universality, intelligence, programmability and resistance to mode crosstalk, environmental disturbances, and fabrication errors, demonstrating that the MZI-based reconfigurable mode processor chip has great potential in long-distance chip-to-chip multimode optical communication systems.","PeriodicalId":93483,"journal":{"name":"PhotoniX","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136262424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1