{"title":"Fetal mummification in a goat","authors":"E. Whitt, Hope Patrick, Pablo Jarrín, Tulio Prado","doi":"10.58292/ct.v15.9410","DOIUrl":"https://doi.org/10.58292/ct.v15.9410","url":null,"abstract":"A 5-year pet doe (unknown breed) was presented (~ 1 month after her expected day of kidding) with a history of vaginal discharge and fetal membranes hanging from the vulva. She was bright, alert, and responsive with an elevated heart rate and respiratory rate. Blood chemistry panel was unremarkable. Vaginal speculum examination was attempted; however, her vulva was 1 inch in length (dorsal to ventral commissure) making it very hard. Transabdominal uterine ultrasonography revealed no fetus and uterus had an irregular echogenicity. A small fetus was identified via transrectal uterine ultrasonography. Decision was to perform cesarean surgery (left flank approach). A mummified fetus was extracted from the left uterine horn.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":"954 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41279341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Blodgett, C. Morrow, Scott Bailey, Jaye L. McCracken, N. Nieman, D. Amodie, KentonL. Morgan
{"title":"Evaluation of an equine multi-antigen rabies combination vaccine in pregnant mares","authors":"G. Blodgett, C. Morrow, Scott Bailey, Jaye L. McCracken, N. Nieman, D. Amodie, KentonL. Morgan","doi":"10.58292/ct.v15.9269","DOIUrl":"https://doi.org/10.58292/ct.v15.9269","url":null,"abstract":"Mares are commonly vaccinated during pregnancy, especially in the late third trimester, with multiple vaccine products containing a variety of antigens. Currently, a new core antigens vaccine containing rabies virus, tetanus toxoid, eastern equine encephalomyelitis virus, western equine encephalomyelitis virus, and West Nile virus with and without the inclusion of Venezuelan equine encephalomyelitis virus is used in later term mares. Use of this vaccine has not been evaluated for safety in pregnant broodmares. We determined the safety of this vaccine in pregnant mares as it pertains to live foaling rate and per cycle pregnancy rate. Findings indicated that the use of a new multi-antigen rabies combination vaccine had no impact on live foaling rate nor per cycle pregnancy rates.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41798565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Diaphragmatic hernia involving gravid uterus in a dog","authors":"M. Ciccarelli, T. Pérez, W. Dernell","doi":"10.58292/ct.v15.9319","DOIUrl":"https://doi.org/10.58292/ct.v15.9319","url":null,"abstract":"A 4-year German Shorthair Pointer pregnant dog was presented for severe tachypnea and depression. She had 1 natural breeding 52 days ago. Diaphragmatic hernia with intrathoracic displacement of the uterus was diagnosed via radiography and ultrasonography. Emergency herniorrhaphy was elected and the owner requested to save the litter if possible. Right uterine horn tip, spleen, part of liver, pancreas, and part of greater omentum were in the thorax. Intraoperative transuterine ultrasonography confirmed live fetuses and pregnancy was permitted to continue. Patient received supportive medical care for 3 days. Based on ultrasonography findings (eminent danger to litter), decision was made to perform an emergency cesarean surgery (56 days after breeding). Six of 11 pups were alive and 1 mummified fetus had an elongated neck (perhaps due to entrapment in the diaphragmatic hernia).","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46075497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Canine transcervical insemination: history and technique","authors":"W. Whitler","doi":"10.58292/ct.v15.9320","DOIUrl":"https://doi.org/10.58292/ct.v15.9320","url":null,"abstract":"Artificial insemination in the dog was first described nearly 250 years ago. Until the 1970s, the process remained predominantly in depositing semen in the cranial vagina. Initially, surgery was required to successfully deposit frozen–thawed semen in uterus. However, societal demands to eliminate the need for general anesthesia and surgery for breeding have led to development of reliable and successful transcervical insemination procedures. Common barriers to perform successful transcervical inseminations include equipment expense and developing skills. In addition to practice, adopting suggestions provided by the author will improve practitioner’s success.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46149144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Vanderwall, Dustin Kinney, Holly Clement, J. Rigas, D. Wilson
{"title":"Effect of human chorionic gonadotropin treatment on the duration of oxytocin-induced prolonged corpus luteum function in mares","authors":"D. Vanderwall, Dustin Kinney, Holly Clement, J. Rigas, D. Wilson","doi":"10.58292/ct.v15.9270","DOIUrl":"https://doi.org/10.58292/ct.v15.9270","url":null,"abstract":"Oxytocin is used increasingly in mares to prolong corpus luteum (CL) function to suppress estrus. When mares develop prolonged CL function in response to oxytocin treatment, the CL generally remains functional for 60–90 days. However, a longer duration of CL function can make this method of estrus suppression even more clinically useful. We hypothesized that human chorionic gonadotropin (hCG) treatment during the period of oxytocin-induced prolonged CL function would extend the duration of CL function (i.e. beyond 90 days). Prolonged CL function was induced in mares (n = 14) by treating them with 60 units of intramuscular oxytocin once daily on days 7–14 after ovulation. Mares were then randomly assigned equally to a control group that received no additional treatment and an hCG-treated group that received 2,500 units of intramuscular hCG on days 30, 45, 60, 75, and 90 after ovulation. Jugular blood samples were collected for progesterone concentration determination on the day of ovulation and then 3 times (M, W, and F) weekly for 120 days. Duration of CL function was not different (p > 0.05) and it was 78.0 ± 7.5 and 91.4 ± 20.4 days (mean ± standard deviation [SD]) in control and hCG-treated mares, respectively. Therefore, hCG treatment (during the period of oxytocin-induced prolonged CL function) did not extend CL function, hence alternative methods should be explored.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45549276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Camille Ogdon, C. Estill, Kelly Van Scoyk, Lauren E Newsom
{"title":"Accuracy of radiographic fetal count in ewes","authors":"Camille Ogdon, C. Estill, Kelly Van Scoyk, Lauren E Newsom","doi":"10.58292/ct.v15.9221","DOIUrl":"https://doi.org/10.58292/ct.v15.9221","url":null,"abstract":"Accurate fetal count is important for farm animal species as the number of fetuses can affect management decisions in both research and production settings. Documented methods of pregnancy diagnosis include radiography, progesterone assays, transrectal palpation, and transrectal and transabdominal ultrasonography; however, there is variability in fetal count accuracy with each of these methods. Abdominal radiography evaluation in 13 pregnant ewes among observers of various skill levels was compared retrospectively with the known number of fetuses determined using computed tomography. Overall accuracy using abdominal radiography across skill levels for determining fetal counts correctly was 79%. Accuracy decreased as the number of fetuses increased, with accuracies for singleton, twin, and triplet pregnancies being 92, 72, and 50%, respectively. Additionally, observer experience was inversely related to radiographic fetal count accuracy.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48885549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Kelley, J. Roscoe, Alex Wittorff, A. Bassett, Fabio Pinaffi, R. Holyoak
{"title":"Intromission failure in a stallion","authors":"D. Kelley, J. Roscoe, Alex Wittorff, A. Bassett, Fabio Pinaffi, R. Holyoak","doi":"10.58292/ct.v14i4.9175","DOIUrl":"https://doi.org/10.58292/ct.v14i4.9175","url":null,"abstract":"Determining the cause of poor libido in stallions can be challenging. Many factors can cause disturbance in libido (e.g., lameness or physical pain, hormonal, age, experience, and handling). Identifying the cause of poor libido is important to determine the best treatment approach. A 6-year Thoroughbred stallion was presented for failure of intromission when attempting to breed mares. Stallion was recently retired from racing. Stallion was placed on hormonal therapy that did not change stallion’s aberrant reproductive behavior. On presentation no obvious physical causes were identified and the stallion displayed masturbation behavior. Through the review of video recordings of the breeding sessions, modifications were made that eventually resulted in the stallion successfully breeding mares without hormonal intervention. This case illustrated how video recordings of breeding activity can help in determining cause of reproductive dysfunction. Furthermore, interpretation of peripheral steroid hormone concentrations needs to be carried out with caution.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70899998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Peyton Draheim, Katelyn C Hlusko, R. Cole, J. Douglas, Cornelius J Withers, E. Graff, R. Wilborn
{"title":"A case of unilateral hydrocele in a dog secondary to inapparent inguinal hernia","authors":"Peyton Draheim, Katelyn C Hlusko, R. Cole, J. Douglas, Cornelius J Withers, E. Graff, R. Wilborn","doi":"10.58292/ct.v14i4.9172","DOIUrl":"https://doi.org/10.58292/ct.v14i4.9172","url":null,"abstract":"A 6-year, male intact Coonhound, was presented for unilateral scrotal enlargement of roughly 3 months’ duration. His left side scrotum was markedly enlarged and edematous and both testes palpated normal. Rapid slide agglutination test (for brucellosis) and SNAP 4DX (IDEXX Laboratories, Inc., Westbrook, ME) test (for tick-borne disease) were negative. Initial scrotal ultrasonography revealed accumulation of anechoic fluid with hyperechoic flecks and swirling surrounding the left testis. On further ultrasonographic examination, omentum appeared extending through the left inguinal ring and into the scrotum. A case of scrotal hernia with secondary hydrocele was diagnosed. Hydrocele was resolved surgically and the dog returned to full athletic function.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48472614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Sucrose and bovine serum albumin in association with dimethylformamide improved sperm parameters of frozen stallion semen","authors":"Madeline Kurpita, J. Len","doi":"10.58292/ct.v14i4.9171","DOIUrl":"https://doi.org/10.58292/ct.v14i4.9171","url":null,"abstract":"Postthaw stallion sperm integrity was determined after freezing semen in an extender supplemented with several sucrose concentrations, bovine serum albumin (BSA), and with and without dimethylformamide (DMF). Two ejaculates from 6 stallions (n =12) were diluted and aliquots (n = 7) were made for treatment groups (25 mM sucrose + BSA [S25], S25 + DMF [S25DMF], 50 mM sucrose + BSA [S50], S50 + DMF [S50DMF], 100 mM sucrose + BSA [S100], S100 + DMF [S100DMF], and Control (only DMF). Semen was frozen in a computer-controlled freezer. Sperm postthaw motility (total and progressive) and kinetics were assessed using CASA. Postthaw sperm plasma membrane and acrosomal integrity were evaluated using SYBR-14/PI and FITC-PNA, respectively. Sperm motility was higher in S100DMF and S50DMF. Plasma membrane integrity was higher in S100DMF, S50DMF, and S100. As sucrose concentration increased, plasma membrane integrity increased. Treatment groups with sucrose and BSA, regardless of DMF, had higher acrosome integrity than Control. Sucrose and BSA in association with DMF in a freezing extender protected sperm integrity during freezing and thawing.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44571458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Lisboa, L. Segabinazzi, F. Hartwig, Camila Freitas-Dell`Aqua, F. Papa, José Dell`Aqua Jr
{"title":"L-carnitine and acetyl-L-carnitine enhance stallion sperm quality during semen storage at 5°C","authors":"F. Lisboa, L. Segabinazzi, F. Hartwig, Camila Freitas-Dell`Aqua, F. Papa, José Dell`Aqua Jr","doi":"10.58292/ct.v14i4.9169","DOIUrl":"https://doi.org/10.58292/ct.v14i4.9169","url":null,"abstract":"Carnitine, a powerful antioxidant, has an essential role in sperm energy metabolism. Among carnitines, only L-carnitine’s effect on stallion semen has been tested and not acetyl-L-Carnitine. Therefore, we aimed to determine the ideal concentrations of L-carnitine (LC) and acetyl-L-carnitine (AC) and their effects on stallion semen cooled at 5℃ for up to 48 hours. Semen was extended to 50 x 106 sperm/ml in commercial extender (Control), and concentrations of 5, 10, and 15 mmol/l of LC and AC were evaluated in Experiment 1. Sperm motility and plasma membrane integrity were assessed by CASA and epifluorescence microscopy, respectively. In Experiment 2, the combination of the intermediate doses of LC (10 mmol/l) and AC (10 mmol/l) was tested. Sperm parameters were evaluated as in Experiment 1 and in addition, DNA fragmentation index (DFI), production of reactive oxygen species (ROS), and lipid peroxidation (PEROX) were evaluated by flow cytometry. All analyses were performed at 0, 24, and 48 hours after semen collection, processing, and cooled-stored at 5°C. In Experiments 1 and 2, the groups supplemented with LC and AC or LC+AC had higher plasma membrane integrity and motility parameters compared to Control group (p < 0.05). The LC and AC combination did not change sperm parameters compared to LC or AC alone (p > 0.05). No differences (p > 0.05) were observed for DFI, ROS, and PEROX. In conclusion, LC and AC’s addition, alone or in combination, enhanced sperm motility and plasma membrane integrity of stallion sperm after cooled-storage at 5℃ for up to 48 hours.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45877160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}