B. Diehl, F. Grosso, Marley E. Iredale, Michael Dark, C. Cabrera
{"title":"Congenital hypospadias and vertebral malformation in a Barbados Blackbelly neonate","authors":"B. Diehl, F. Grosso, Marley E. Iredale, Michael Dark, C. Cabrera","doi":"10.58292/ct.v14.9295","DOIUrl":"https://doi.org/10.58292/ct.v14.9295","url":null,"abstract":"A male purebred Barbados Blackbelly, twin to another male, was born with obvious external congenital abnormalities. Male twinwas normal. Abnormal lamb had a short torso and spinal column. At 1 week, the lamb was presented with dyspnea and lethargy,and euthanasia was elected. Lamb was submitted for full necropsy and for additional diagnostics. After postmortem, in additionto histopathology, radiographs and computed tomographs were obtained. Lamb had congenital vertebral and occipital malformations,meningitis, hypospadias, and renal malformation. To authors’ knowledge, hypospadias and vertebral malformation inthe Barbados Blackbelly breed has not been reported. Apparently, Barbados Blackbelly breed harbors closely related genetics, thusvigilant monitoring for genetic malformations is suggested.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41448002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Avian reproductive tract diseases and surgical resolutions","authors":"S. Echols, B. Speer","doi":"10.58292/ct.v14.9297","DOIUrl":"https://doi.org/10.58292/ct.v14.9297","url":null,"abstract":"Reproductive tract disease is a commonplace in avian practice, particularly among female birds. Unique anatomy and physiologyof the avian reproductive tract results in a highly productive but poorly accessible organ system. Affected hens are commonly presentedwith nonspecific lethargy, coelomic swelling and sometimes laying abnormal eggs. Reproductive diseases are less commonin male birds; however, they are well described. Although many reproductive tract diseases can be managed medically, there isa definite need for surgical intervention in some cases. Because of internal location, various disease processes and even normalphysiologic processes can dramatically alter patient presentations. For this reason, a clear understanding of avian female anatomyand male reproductive tracts is vital to diagnose and treat disorders. This manuscript focuses on recognition and treatment(emphasizing surgical management) of avian reproductive tract diseases. Because of the unique avian reproductive anatomy andfrequent need for surgical correction of disorders, the organization of the manuscript starts with descriptions of coelom and basicsurgical principles.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43896543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hannah Ruetten, Marlyse Wehber, Mary Murphy, Clara Cole, Simran Sandhu, Steven Oakes, Dale Bjorling, Kenneth Waller, Katrina Viviano, Chad Vezina
{"title":"A retrospective review of canine benign prostatic hyperplasia with and without prostatitis.","authors":"Hannah Ruetten, Marlyse Wehber, Mary Murphy, Clara Cole, Simran Sandhu, Steven Oakes, Dale Bjorling, Kenneth Waller, Katrina Viviano, Chad Vezina","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Benign prostatic hyperplasia (BPH) is the most common prostatic disorder in older intact male dogs, but despite its prevalence, there are inconsistencies in clinical diagnosis and treatment. Although prostate size was historically considered the hallmark feature of BPH in men, currently, there is only a weak correlation between prostate size and clinical severity. We performed a retrospective cohort study with the primary objective of assessing clinical signs, ultrasonographic findings, treatments, and outcomes in dogs diagnosed with BPH, with and without concurrent prostatitis. We reviewed medical records and obtained data on presenting signs, prostatic imaging, and prevalence of concurrent bacteriuria. Prostate size was determined by ultrasonography and compared to the calculated expected size based on patient age and weight. Treatment and outcome were described for the cases with a minimum 2 months follow-up. Median age of dogs diagnosed with BPH was 8 years. Clinical signs were present in 16/25 dogs and scored as mild to moderate (median Zambelli's Symptom Index for BPH score 12). The median prostatic volume to body mass ratio was 1.60 mm<sup>3</sup>/kg. Prostate size did not correlate with the symptom severity. Concurrent bacteriuria was confirmed in 4/25 cases via bacterial culture and/or cytology. Treatments pursued and responses were only available in a subpopulation of dogs (n = 9) and were highly variable. Studies are needed to determine if current treatment options for BPH in dogs resolve associated clinical signs in addition to reducing prostate size.</p>","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":"13 4","pages":"360-366"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8782267/pdf/nihms-1768784.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39962781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Carolyn Chisholm, K. Walters, D. Christiansen, H. King, R. Hopper, Darcie R. Sidelinger
{"title":"Vaginal prolapse in an American Quarter Horse mare","authors":"Carolyn Chisholm, K. Walters, D. Christiansen, H. King, R. Hopper, Darcie R. Sidelinger","doi":"10.58292/therio.v13i.9101","DOIUrl":"https://doi.org/10.58292/therio.v13i.9101","url":null,"abstract":"A 15 year old, multiparous, American Quarter Horse mare in late pregnancy, was presented for a mass protruding from the vagina that was noticed 12 hours ago. Physical examination revealed a large, 25 x 12.5 x 12.5 cm, pink to red vaginal protrusion with moderate amounts of mucoid discharge. On transrectal palpation, it was evident that the mare was carrying a live fetus. Reminder of the physical examination was unremarkable. Vaginal prolapse was diagnosed that was manually reduced and an episioplasty procedure was performed. Mare was discharged with instructions to be monitored closely for 14 days and then re-evaluated, unless there were signs of impending parturition. Owner was instructed to seek immediate veterinary care if signs of parturition were noted. Foaling occurred without the knowledge of the owner, resulting in labial tear. Mare and a premature foal were presented at the hospital. Foal died during treatment. Mare was euthanized months later due to unrelated lameness. To authors’ knowledge, this is the first case report of a vaginal prolapse in a mare.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46396414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Sperm acrosome associated 3 protein expression in equine primordial, primary, secondary, and tertiary follicles","authors":"Brynley C. Cozzi, Zahra Kiesler, M. Kutzler","doi":"10.58292/therio.v13i.9100","DOIUrl":"https://doi.org/10.58292/therio.v13i.9100","url":null,"abstract":"Granulosa cellThe objective of this study was to characterize the protein expression of sperm acrosome associated 3 (SPACA3) in the equine ovary. Formalin-fixed and paraffin-embedded ovarian sections from 16 horses were processed for routine immunohistochemistry for SPACA3. Representative images were digitally captured at 400 x magnification. In all mares, SPACA3 was expressed in granulosa cells of all stages of follicles. Expression of SPACA3 in all equine follicular stages suggests that this may be a permanent immunosterilant target for the management of feral horse herds. Additional research is needed to determine if horses can produce a robust humoral response to a SPACA3 vaccine to induce sustained infertility.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43920493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fabio Pinaffi, R. Wilborn, Lindsey R. Boone, A. Johnson
{"title":"Effect of platelet rich plasma lysate and fibroblast growth factor 2 on stallion sperm motility","authors":"Fabio Pinaffi, R. Wilborn, Lindsey R. Boone, A. Johnson","doi":"10.58292/ct.v13.9357","DOIUrl":"https://doi.org/10.58292/ct.v13.9357","url":null,"abstract":"Growth factors (GFs) are known to modulate cell function and their presence in semen could be advantageous to sperm. In humans andmice, fibroblast growth factor 2 (FGF2) improved semen motility. Platelet rich plasma (PRP), rich in GFs including FGF2, reduced postmating inflammatory response within the uterus; however, its effects on sperm are not known. PRP lysate (PRPL) is much purer than PRPand contains higher concentrations of GFs. Hence, the objective of the present study was to evaluate the effect of supplementing freshand cool-stored stallion sperm with either PRPL at 1, 2.5, 5, and 10% (also containing 1 IU/ml of heparin), or FGF2 at 0.1, 1, 10, and100 ng/ml. Motility parameters were evaluated using computer assisted semen analysis at 0, 0.5, 1, 1.5, 6, and 24 hours after treatment.For both PRPL and FGF2 treatments, there were no differences in total and progressive motility among groups. Concentrations of PRPL> 5% induced sperm agglutination via head-to-head attachment, starting at hour 1 and was more pronounced for 10% PRPL than 5%PRPL, suggesting a dose-dependent characteristic. Direct addition of PRPL to semen extender at doses < 5% might not substantiallyaffect sperm motility, whereas doses > 5% might affect sperm motility due to head-to-head attachments. Addition of FGF2 at theconcentrations studied may not affect sperm characteristics.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49103333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Katie Withowski, Alyssa B Helms, Victor Stora, K. Meurs
{"title":"Veterinary genetic counseling service: a preliminary report","authors":"Katie Withowski, Alyssa B Helms, Victor Stora, K. Meurs","doi":"10.58292/ct.v13.9355","DOIUrl":"https://doi.org/10.58292/ct.v13.9355","url":null,"abstract":"Data generated from an online veterinary genetics counseling service from November 1, 2018 to October 31, 2019 were reviewed. Datawere tabulated regarding the individual submitting the inquiry and relationship to the pet, species, breed, and medical issue(s) thoughtto be familial. Inquiries were assigned to 1 or more of the following: risk assessment and heritability, pre-breeding testing and pedigreereview, and information regarding a specific mutation or minor health concern. This study demonstrated that clients are desirous forinformation that could help promote health in canine companions. Furthermore, there is an interest and need for additional geneticresources for both veterinarians and owners.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49381528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of sunlight hours and hormones on double ovulation, and singleton and twin pregnancies in mares","authors":"Martha A Mellish, Kasadee Allan, Bronwyn Crane","doi":"10.58292/ct.v13.9356","DOIUrl":"https://doi.org/10.58292/ct.v13.9356","url":null,"abstract":"Equine twin pregnancies are almost exclusively dizygotic, without the application of advanced reproductive technologies, requiring 2ovulations in 1 estrous cycle. Breeding records were used to determine the effects of sunlight hours, prostaglandin F2α, human chorionicgonadotropin, deslorelin (a gonadotropin releasing hormone agonist), and progesterone and estradiol on double ovulation rates,and singleton and twin pregnancy rates. Breeding records of mares (n = 267) and their estrous cycles (n = 914) were analysed. Doubleovulations occurred in 10.5% (96/914) of estrous cycles. Twin pregnancies were observed in 42.7% (38/89) of mares that had doubleovulations. Overall, per estrous cycle pregnancy rate was 47.2% (405/858) and twin pregnancies was 4.4% (38/858). Double ovulationshad higher (p < 0.001) per cycle singleton pregnancy rate (69.7%; 62/89) than 1-ovulation cycles (44.6%; 343/769). Deslorelinincreased (p < 0.05; OR =1.24 95% CI) double ovulations and human chorionic gonadotropin tended (p = 0.089; OR =1.68; 95%CI) to increase double ovulations. Deslorelin use resulted in an odds ratio of 2.47 for a positive pregnancy (either singleton or twin)diagnosis compared to cycles without deslorelin use. None of the factors examined had a substantial impact on twin pregnancy rates.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47538798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Augustine T. Peter, L. Brito, G. Althouse, C. Aurich, P. Chenoweth, Natalie Fraser, C. Lopate, C. Love, G. Luvoni, D. Waberski
{"title":"Andrology laboratory review: evaluation of sperm motility","authors":"Augustine T. Peter, L. Brito, G. Althouse, C. Aurich, P. Chenoweth, Natalie Fraser, C. Lopate, C. Love, G. Luvoni, D. Waberski","doi":"10.58292/ct.v13.9359","DOIUrl":"https://doi.org/10.58292/ct.v13.9359","url":null,"abstract":"This review is an effort by the andrology taskforce of the Association of Applied AnimalAndrology, American College of Theriogenologists, European College of Animal Reproduction, andSociety for Theriogenology. It is intended to serve as a reference on methods to evaluate spermmotility in domestic animals and to contribute to adoption of best practices in veterinary andrologylaboratories and semen processing centers. Sperm motility evaluation topics covered include samplepreparation, subjective evaluation, computer-assisted semen analysis, and use of sperm qualityanalyzers. Emphasis is given to principles of the methods, equipment, performing evaluation, andcommon mistakes and/or pitfalls. In addition, precision and accuracy of the various methods arediscussed.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43045445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Single layer density centrifugation improved semen quality in a stallion with hydrocele","authors":"S. Macedo, Ana Filipe, T. Guimarães, A. Rocha","doi":"10.58292/ct.v13.9363","DOIUrl":"https://doi.org/10.58292/ct.v13.9363","url":null,"abstract":"Hydrocele was diagnosed in a 21 year old stallion with a history of subfertility. Subjective progressive motilityand normal sperm from ejaculates (n = 12) were (mean ± SEM) 25 ± 11% and 24 ± 7%, respectively. From eachejaculate,1 sample was centrifuged through a silica colloid and refrigerated for 72 hours. An uncentrifuged sampleserved as control. Morphologically normal sperm was higher (p < 0.05) in semen centrifuged through the silicacolloid. Sperm motility in cool-stored semen was higher (p < 0.05) in silica colloid centrifuged semen compared tocontrol at 24, 48, and 72 hours postrefrigeration. Percent viable sperm and sperm with high mitochondria membranepotential were higher (p < 0.05) at 24 hours of postrefrigeration for semen centrifuged through silica colloid. Threemares were inseminated at alternate estrus, either with semen centrifuged through the silica colloid or with control.Inseminations (n = 3) with uncentrifuged semen (≥ 500 x 106 motile sperm) did not result in pregnancy. However,inseminations with fresh semen (n = 2) or 24 hours chilled semen (n = 2) obtained after centrifugation with singlelayer colloid (≥ 170 x 106 motile sperm) resulted in pregnancies. We concluded that hydrocele had apparentlycontributed to decreased semen quality and fertility in this stallion, and centrifugation of the ejaculate through a silicacolloid improved semen quality.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42980131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}