{"title":"What has embryo recovery and transfer taught us about equine reproductive physiology?","authors":"Sandra Wilsher, Maarten Hoogewijs","doi":"10.58292/ct.v16.10602","DOIUrl":"https://doi.org/10.58292/ct.v16.10602","url":null,"abstract":"Equine embryo transfer has been the foundation stone on which modern reproductive technologies and novel research in this species has been built. Nonsurgical embryo recovery and transfer methods developed from the original surgical methods, and the ease with which these procedures can be undertaken has enabled these techniques to play a continuing role in original research. We document how embryo recovery and/or transfer have increased our knowledge about many of the events involved in equine reproductive physiology. These include, when the equine embryo enters the uterus, the importance and development of the blastocyst capsule, secretory products of early embryos, early maternal-embryo interactions, effects of uterine asynchrony on early embryo development, the role of endometrial cups and maternal immunological tolerance of extraspecies embryo transfers, and the influence of uterine environmental on fetal growth and development.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":"22 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141815573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nicole Sugai, Clifford Shipley, Lindsey Rothrock-Gensler, Robyn Ellerbrock, Jamie Stewart
{"title":"Laparoscopic artificial insemination of white-tailed deer with fresh sex-sorted semen","authors":"Nicole Sugai, Clifford Shipley, Lindsey Rothrock-Gensler, Robyn Ellerbrock, Jamie Stewart","doi":"10.58292/ct.v16.10513","DOIUrl":"https://doi.org/10.58292/ct.v16.10513","url":null,"abstract":"This case study describes the feasibility of using chilled, sex-sorted semen for laparoscopic artificial insemination (AI) in the captive white-tailed deer (Odocoileus virginianus) industry. Ejaculates from 3 bucks were collected, extended, and shipped overnight for flow cytometric sex-sorting. Sex-sorted semen was chilled and shipped back overnight. Synchronized does were bred using chilled, sex-sorted (n = 30, farm 1; n = 10, farm 2) or conventional frozen (n = 7, farm 2) semen by laparoscopic AI. Does were subsequently turned out with bucks. Percentage of bucks born via AI with sex-sorted semen was 85% (28/33) for farm 1 and 100% (16/16) for farm 2. Percentage of bucks born by natural cover was 50% (12/24) for farm 1 and percentage of bucks born by using either conventional semen or live cover was 56% (9/14) for farm 2. This case study demonstrated acceptable results using chilled, sex-sorted semen in white-tailed deer herds as a technique to manipulate the sex proportions per breeding season.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":"20 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141815720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maria Ferrer, Roberto Palomares, Juan Maldonadox-Estrada
{"title":"Role of trace minerals in bull reproductive physiology and semen quality","authors":"Maria Ferrer, Roberto Palomares, Juan Maldonadox-Estrada","doi":"10.58292/ct.v16.10351","DOIUrl":"https://doi.org/10.58292/ct.v16.10351","url":null,"abstract":"Testicular dysfunction is often associated with an imbalance in antioxidant/oxidant homeostasis, leading to negative effects of oxidative stress on germ cell proliferation, steroidogenesis, and sperm function. Trace minerals (TM) are involved in basic homeostatic and enzymatic processes like free radical detoxification, cellular respiration, carbohydrates, lipids, nucleic acids, synthesis and metabolism of proteins, and stabilization of membranes and DNA. Dietary source of TM is necessary to support these processes. Supplementing cattle with TM is nowadays a common practice to help support growth, reproduction, and immunity. This review provides information on roles of TM in bulls testicular and sperm functions, and effects of TM mineral supplementation on bull reproductive parameters.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":"10 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141119499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eduardo Prado, Tulio Prado, Elizabeth Whitt, Alison Bradley, P. Mulon
{"title":"Vaginal lipoma with cervicovaginal and rectal prolapse in a cow","authors":"Eduardo Prado, Tulio Prado, Elizabeth Whitt, Alison Bradley, P. Mulon","doi":"10.58292/ct.v16.10269","DOIUrl":"https://doi.org/10.58292/ct.v16.10269","url":null,"abstract":"A 5-year, mixed breed cow, was presented with cervicovaginal and rectal prolapses. Cow had a vaginal mass on the left caudal aspect of cervicovaginal prolapse. Rectal prolapse was resolved; however, vaginal mass prevented reduction of vaginal prolapse. Next day, vaginal mass was resected that made vaginal prolapse reduction and vulval closure (Bühner stich) possible. Histopathology identified the mass as infected vaginal lipoma.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":" 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140998414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ali Bazzazan, D. Vallejo-Timarán, Juan Maldonado-Estrada, Mariela Segura, Réjean Lefebvre
{"title":"Diagnosis of clinical cervicitis and vaginitis in dairy cows in relation to various postpartum uterine disorders","authors":"Ali Bazzazan, D. Vallejo-Timarán, Juan Maldonado-Estrada, Mariela Segura, Réjean Lefebvre","doi":"10.58292/ct.v16.10309","DOIUrl":"https://doi.org/10.58292/ct.v16.10309","url":null,"abstract":"There is poor agreement between clinical findings and results of diagnostic tests in postpartum uterine diseases that may be explained at least in part by the presence of inflammation and infection at sites other than the endometrium. It was hypothesized that clinical cervicitis and vaginitis are components of reproductive tract inflammatory disorders in postpartum dairy cows. A total of 61 postpartum dairy cows were enrolled in a nested case-control design. Periparturient disease occurrence in weeks 1 (1w), 3 (3w), and 5 (5w) postpartum were recorded. Clinical cervicitis was diagnosed in 36.0, 40.1, and 31.1% of cows in 1w, 3w, and 5w postpartum, respectively. Approximately 64% of cows with clinical endometritis in 5w postpartum also had clinical cervicitis (p ≤ 0.05). Prevalence of clinical and cytological vaginitis was 0% in 5w postpartum. Average days open: 110 for healthy cows and 117 for cows with clinical cervicitis (p ≤ 0.001), 145 for cows with clinical cervicitis and clinical endometritis (p < 0.005), and 199 for cows with clinical cervicitis and cytological endometritis (p < 0.001). Contrary to vaginitis, high prevalence of clinical cervicitis and its association with clinical endometritis and longer average days open suggested a substantial role of the condition in postpartum uterine diseases.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":"357 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141006804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rory Nevard, Marco Lopes, Heidi Lehmann, Chris Quinn, Cassandra MacDonald
{"title":"Modified partial posthectomy surgery for chronic preputial prolapse in a Bos indicus bull","authors":"Rory Nevard, Marco Lopes, Heidi Lehmann, Chris Quinn, Cassandra MacDonald","doi":"10.58292/ct.v16.10415","DOIUrl":"https://doi.org/10.58292/ct.v16.10415","url":null,"abstract":"Preputial prolapses in bulls are frequently encountered by bovine reproductive practitioners and are common physical conditions impairing a bull’s ability to copulate. Marked chronicity or unsuccessful medical management warrant surgical correction. Preputial resection/circumcision (also known as ‘reefing procedure’) or preputial amputation are 2 well-documented procedures performed to restore penile function. It is widely believed that preputial resection is considered superior to amputation, as with the latter procedure, strictures and wound contracture stenosis are common reported sequelae. Therefore, surgery should aim at preserving tissue and resolving the prolapse without interference to complete penile extension. We adapted novel surgical features in a bull with a chronically prolapsed prepuce: an updated preputial amputation technique (i.e. modified posthectomy) and used a single-layer closure pattern with rapidly absorbable suture (not reported for bulls). There was total resolution and bull returned to service. This case illustrated that severe and chronically prolapsed prepuces can be successfully repaired using this updated modification of a historical technique.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141007316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kent Weigel, Agustin Chasco, H. Pacheco, Anil Sigdel, Fiona Guinan, Megan Lauber, Paul Fricke, Francisco Peñagaricano
{"title":"Genomic selection in dairy cattle: impact and contribution to the improvement of bovine fertility","authors":"Kent Weigel, Agustin Chasco, H. Pacheco, Anil Sigdel, Fiona Guinan, Megan Lauber, Paul Fricke, Francisco Peñagaricano","doi":"10.58292/ct.v16.10399","DOIUrl":"https://doi.org/10.58292/ct.v16.10399","url":null,"abstract":"Genomic selection has revolutionized the dairy cattle breeding industry, with ripple effects that have greatly impacted dairy herd management. Rate of genetic progress has increased markedly, especially in Holstein and Jersey breeds, for production, health, and fertility traits. Genomic testing of young bulls and heifers provides greater accuracy of selection decisions involving traditional fertility traits, such as daughter pregnancy rate, while creating the opportunity to improve novel traits, such as fetal loss. Cameras, wearable sensors, and other precision livestock farming technologies will allow selection for traits such as estrus duration and intensity that require high frequency phenotyping. At the same time, synergies between genomic testing and advanced reproductive technologies have led to rapid and widespread adoption of sexed semen, coupled with mating of females whose offspring are not needed as herd replacements to beef sires. This strategy produces added-value crossbred calves for the beef supply chain, while allowing genetically inferior mature cows that are still producing at a high level to remain in the herd for additional lactations.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140242968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. McRae, Marco Coutinho da Silva, E. Runcan, Joanna Koilpillai, Christopher Premanandan
{"title":"Congenital hydrocephalus in a stillborn Haflinger foal","authors":"G. McRae, Marco Coutinho da Silva, E. Runcan, Joanna Koilpillai, Christopher Premanandan","doi":"10.58292/ct.v16.10452","DOIUrl":"https://doi.org/10.58292/ct.v16.10452","url":null,"abstract":"A 14-year, multiparous Haflinger mare, apparently fullterm (unknown breeding or ovulation date), was presented for dystocia. Mare was transvaginally palpated at the farm by the referring veterinarian; foal was in craniolongitudinal presentation, dorsosacral position with extended forelimbs in the vaginal vault and head just cranial to cervix, and a cranial abnormality of the foal prevented assisted vaginal delivery. Mare was referred. Foal was not alive at presentation and was undeliverable with assistance because of congenital hydrocephalus. Anesthetized mare was placed in Trendelenburg position, controlled vaginal delivery was elected and foal was removed via fetotomy. Mare was negative for β1, 3- N-acetylgalactosaminyltransferase 2 (B3GALNT2) nonsense mutation (reported to be responsible for hydrocephalus). Dystocia in a Haflinger mare because of hydrocephalus is reported for the first time.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":"16 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140242703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mariana P Mazzuchini, Guilherme Novello, Lorenzo Segabinazzi
{"title":"Modified diff-quick staining for canine sperm morphology","authors":"Mariana P Mazzuchini, Guilherme Novello, Lorenzo Segabinazzi","doi":"10.58292/ct.v16.10243","DOIUrl":"https://doi.org/10.58292/ct.v16.10243","url":null,"abstract":"Sperm morphology assessment requires specialized microscopes or stains. Diff-quick (DQ) is considered a universal stain that is cost-effective; however, morphological evaluation of sperm using DQ staining is poor and not encouraging. Therefore, this study investigated modifications to the DQ protocol to improve identification of morphological defects of dog sperm and compared the modified DQ techniques with eosin-nigrosin, Karras, and differential interference contrast microscopy [DIC]). One ejaculate from each of 9 dogs was used. To perform the proposed modified DQ techniques (DQ1 and DQ2), dried semen smears were fixed by immersing for 10 seconds in solution 1 of DQ and 5 minutes each in solutions II and III of the kit. After the third stain solution, slides in DQ1 were rinsed in water whereas slides in DQ2 were not rinsed but were vertically supported to facilitate stain drainage. Results suggested that the standard DQ protocol overestimated normal sperm and detached heads whereas underestimated abnormal heads and total defects compared to DIC, Karras, eosin-nigrosin, and DQ2. Acrosome abnormalities were only detectable with Karras, DIC, and DQ2. In conclusion, prolonging exposure to DQ staining solutions enhanced sensitivity in sperm morphological evaluation, and avoiding rinse as a final step in the DQ protocol improved visualization of certain acrosome defects in dog sperm. Therefore, modified DQ techniques can serve as a viable alternative for dog sperm morphology evaluation in clinical practice.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":"37 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139961857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Amorphus globosus","authors":"Kaylin McNulty, Michael Jaffe, Joanne Yi","doi":"10.58292/ct.v16.10328","DOIUrl":"https://doi.org/10.58292/ct.v16.10328","url":null,"abstract":"Amorphus globosus is a rare malformation that is defined as an acardiac asymmetrical rough spherical mass of tissue covered in skin. To authors’ knowledge, amorphus globosus has not been previously reported in canids. Herein, we describe amorphus globosus in an American Bulldog pup with accompanying radiograph, gross images, and histopathology. Additionally, a systematic review (preferred reporting items for systematic reviews and meta-analyses protocol) was performed for veterinary species; amorphus globosus has been reported in 22 bovids, 3 caprids, 1 ovid, and 1 equid.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":"42 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139961907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}