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Effects of bovine ova density and culture supplements on cleavage and blastocyst development rates of in vitro embryos 牛卵子密度和培养辅料对体外胚胎裂解率和囊胚发育率的影响
Clinical theriogenology Pub Date : 2024-02-02 DOI: 10.58292/ct.v16.9801
J. Looman, Zowie Rodriguez, Lyric Waugh, John Gibbons
{"title":"Effects of bovine ova density and culture supplements on cleavage and blastocyst development rates of in vitro embryos","authors":"J. Looman, Zowie Rodriguez, Lyric Waugh, John Gibbons","doi":"10.58292/ct.v16.9801","DOIUrl":"https://doi.org/10.58292/ct.v16.9801","url":null,"abstract":"An optimized in vitro culture system is important to maximize bovine blastocyst development. Four experiments were conducted to explore manipulation of culture environments and investigate the benefits of culturing embryos in groups. Follicles were aspirated from abattoir-derived ovaries and selected oocytes were matured, fertilized, vortexed, and randomly placed in culture groups. Experiment 1 evaluated the effects of ova culture density on blastocyst development among embryos cultured individually or in groups of 5, 10, 20, or 50 embryos. Experiment 2 evaluated the effects of conditioned media from previous replicates on embryo development of the current culture. In Experiment 3, cleaved embryos were grouped together after being in culture for 24 hours. Lastly, in Experiment 4, grouped embryos were placed in 10 μl culture drops to evaluate the effect of high density/low media volume on development. Cleavage and blastocyst rates analyzed via Chi-square indicated that although cleavage rates were similar among culture groups (and experiments), blastocyst development was lower (p < 0.05) in 1 embryo culture group compared to other groups. When conditioned culture media from previous replicates were added to original culture media, blastocyst development was similar among original and conditioned culture groups of 20 and 50 embryos. When cleaved embryos were amalgamated and cultured, blastocyst development was higher (p < 0.05) in culture groups of 10 than 1 cleaved embryo groups but similar to controls for both culture groups. When embryos were cultured in 10 μl drops, embryos cultured in groups of 5 had lower development to blastocysts compared to groups of 2, 10, 25, and control. In conclusion, these data indicated an apparent ‘helper effect’ expressed in culture environments of groups, and this effect apparently occurred after cleavage but before blastocyst development. Direct or indirect role(s) that additional cells have on in vitro culture and the mechanism of this helper effect requires further investigation.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":"25 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139808847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of bovine ova density and culture supplements on cleavage and blastocyst development rates of in vitro embryos 牛卵子密度和培养辅料对体外胚胎裂解率和囊胚发育率的影响
Clinical theriogenology Pub Date : 2024-02-02 DOI: 10.58292/ct.v16.9801
J. Looman, Zowie Rodriguez, Lyric Waugh, John Gibbons
{"title":"Effects of bovine ova density and culture supplements on cleavage and blastocyst development rates of in vitro embryos","authors":"J. Looman, Zowie Rodriguez, Lyric Waugh, John Gibbons","doi":"10.58292/ct.v16.9801","DOIUrl":"https://doi.org/10.58292/ct.v16.9801","url":null,"abstract":"An optimized in vitro culture system is important to maximize bovine blastocyst development. Four experiments were conducted to explore manipulation of culture environments and investigate the benefits of culturing embryos in groups. Follicles were aspirated from abattoir-derived ovaries and selected oocytes were matured, fertilized, vortexed, and randomly placed in culture groups. Experiment 1 evaluated the effects of ova culture density on blastocyst development among embryos cultured individually or in groups of 5, 10, 20, or 50 embryos. Experiment 2 evaluated the effects of conditioned media from previous replicates on embryo development of the current culture. In Experiment 3, cleaved embryos were grouped together after being in culture for 24 hours. Lastly, in Experiment 4, grouped embryos were placed in 10 μl culture drops to evaluate the effect of high density/low media volume on development. Cleavage and blastocyst rates analyzed via Chi-square indicated that although cleavage rates were similar among culture groups (and experiments), blastocyst development was lower (p < 0.05) in 1 embryo culture group compared to other groups. When conditioned culture media from previous replicates were added to original culture media, blastocyst development was similar among original and conditioned culture groups of 20 and 50 embryos. When cleaved embryos were amalgamated and cultured, blastocyst development was higher (p < 0.05) in culture groups of 10 than 1 cleaved embryo groups but similar to controls for both culture groups. When embryos were cultured in 10 μl drops, embryos cultured in groups of 5 had lower development to blastocysts compared to groups of 2, 10, 25, and control. In conclusion, these data indicated an apparent ‘helper effect’ expressed in culture environments of groups, and this effect apparently occurred after cleavage but before blastocyst development. Direct or indirect role(s) that additional cells have on in vitro culture and the mechanism of this helper effect requires further investigation.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":"48 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139868761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of incorrect storage of bull semen samples on sperm morphology assessment 不正确储存公牛精液样本对精子形态评估的影响
Clinical theriogenology Pub Date : 2024-02-01 DOI: 10.58292/ct.v16.9972
Allan Gunn, Mark Baker
{"title":"Effect of incorrect storage of bull semen samples on sperm morphology assessment","authors":"Allan Gunn, Mark Baker","doi":"10.58292/ct.v16.9972","DOIUrl":"https://doi.org/10.58292/ct.v16.9972","url":null,"abstract":"Thirteen pairs of bull semen samples were assessed. One of each pair of samples was inadvertently stored in phosphate buffered saline (PBS) that was used for analysis of motility immediately after collection. Other sample of the pair that was correctly stored in buffered formol saline (BFS) was assessed later. Proportion of morphologically normal sperm was different (p = 0.0021) between the 2 storage methods. Most of the abnormalities in the PBS samples were loose and detached heads. Closer examination of these sperm illustrated changes in the composition of the tail, with an apparent loss of the plasma membrane. This serendipitous error allowed documentation of the importance of correct storage of semen samples for morphological assessment of sperm, and the tertiary defects detected with the incorrect storage of semen samples in semen extender. Tertiary defects should always be considered a possibility during morphological assessment of sperm via spermiogram.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":"33 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139812501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of incorrect storage of bull semen samples on sperm morphology assessment 不正确储存公牛精液样本对精子形态评估的影响
Clinical theriogenology Pub Date : 2024-02-01 DOI: 10.58292/ct.v16.9972
Allan Gunn, Mark Baker
{"title":"Effect of incorrect storage of bull semen samples on sperm morphology assessment","authors":"Allan Gunn, Mark Baker","doi":"10.58292/ct.v16.9972","DOIUrl":"https://doi.org/10.58292/ct.v16.9972","url":null,"abstract":"Thirteen pairs of bull semen samples were assessed. One of each pair of samples was inadvertently stored in phosphate buffered saline (PBS) that was used for analysis of motility immediately after collection. Other sample of the pair that was correctly stored in buffered formol saline (BFS) was assessed later. Proportion of morphologically normal sperm was different (p = 0.0021) between the 2 storage methods. Most of the abnormalities in the PBS samples were loose and detached heads. Closer examination of these sperm illustrated changes in the composition of the tail, with an apparent loss of the plasma membrane. This serendipitous error allowed documentation of the importance of correct storage of semen samples for morphological assessment of sperm, and the tertiary defects detected with the incorrect storage of semen samples in semen extender. Tertiary defects should always be considered a possibility during morphological assessment of sperm via spermiogram.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":"26 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139872357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A note of appreciation 感谢信
Clinical theriogenology Pub Date : 2024-01-25 DOI: 10.58292/ct.v15.10521
The Clinical Theriogenology Editorial Team
{"title":"A note of appreciation","authors":"The Clinical Theriogenology Editorial Team","doi":"10.58292/ct.v15.10521","DOIUrl":"https://doi.org/10.58292/ct.v15.10521","url":null,"abstract":"","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":"24 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139596180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Disorders of sexual development in small ruminants 小反刍动物的性发育障碍
Clinical theriogenology Pub Date : 2024-01-23 DOI: 10.58292/ct.v16.10273
Joaquin Paredes, Lukasz Czochara, Daniel Villagomez, Allan King
{"title":"Disorders of sexual development in small ruminants","authors":"Joaquin Paredes, Lukasz Czochara, Daniel Villagomez, Allan King","doi":"10.58292/ct.v16.10273","DOIUrl":"https://doi.org/10.58292/ct.v16.10273","url":null,"abstract":"Intersex conditions, also known as disorders of sexual development (DSD), are uncommon in small ruminants. This report describes in detail, 2 cases of DSD in small ruminants, including clinical presentation, histopathological and cytogenetic analysis that led to the final diagnoses. Case 1, a mixed-breed ewe, was assessed due to a reported ram-like behavior at ~ 6 months of age. Physical examination revealed abnormal external genitalia with enlarged clitoris and bilateral inguinal gonads, histologically confirmed as testicular tissue. Karyotyping revealed sex chromosome blood chimerism (54XX, 54XY), implicating freemartinism as the cause of DSD. Case 2 was a 1-year Nigerian Dwarf cryptorchid male goat presented for castration. During surgery, bilateral ovoid gonads attached to a bicornuate uterus were identified. Histology revealed testicular tissue and uterus alongside vas deferens were consistent with persistent Müllerian duct syndrome. This report describes presentations, findings, and features of DSD, a rare occurrence in small ruminants.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":"41 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139602614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Stallion sperm concentration measurements: experience and equipment 公马精子浓度测量：经验与设备
Clinical theriogenology Pub Date : 2024-01-22 DOI: 10.58292/ct.v16.10071
Dale Kelley, Reed Holyoak
{"title":"Stallion sperm concentration measurements: experience and equipment","authors":"Dale Kelley, Reed Holyoak","doi":"10.58292/ct.v16.10071","DOIUrl":"https://doi.org/10.58292/ct.v16.10071","url":null,"abstract":"Accuracy is paramount in evaluating sperm concentration and can be a challenge to those with minimal laboratory experience. Purpose of the study was to determine the effect of operator experience on sperm concentrations using 4 methods: Makler® counting chamber, equine densimeter, iSperm, and NucleoCounter®. There was no difference (p = 0.64) between experienced and novice processors for Makler® counting chamber; was difference (p = 0.005) for equine densimeter; was no difference (p = 0.35) for iSperm; and was tending toward difference (p = 0.068) for NucleoCounter®. Correlation between bias and magnitude for Makler® counting chamber was –0.74 (p = 0.003); for equine densimeter was –0.44 (p = 0.11); for iSperm was 0.06 (p = 0.83), and NucleoCounter® was –0.52 (p = 0.06). Makler® counting chamber produced a mean sperm concentration similar to an experienced processor but had significant variation within novice processors. Equine densimeter significantly overestimated sperm concentrations with novice processors but had the least variation. The iSperm performed poorly for both experienced and novices and produced significantly different concentrations than other methods. Thus, iSperm cannot be recommended to accurately measure sperm concentration. Lastly, NucleoCounter had no difference in mean sperm concentrations or variation and was the best system for a novice processor to gain accurate and repeatable sperm concentration measurements.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":"11 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139608465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Canine semen processing for chilled shipment 犬类精液冷藏运输处理
Clinical theriogenology Pub Date : 2023-12-29 DOI: 10.58292/ct.v15.10227
Julie Cecere, Nicole J. Sugai
{"title":"Canine semen processing for chilled shipment","authors":"Julie Cecere, Nicole J. Sugai","doi":"10.58292/ct.v15.10227","DOIUrl":"https://doi.org/10.58292/ct.v15.10227","url":null,"abstract":"Advanced reproductive techniques for dogs have become commonplace in general practice during last decade. Most procedures performed in these practices are based on what is known for semen processing and shipping in the equine industry. This presentation reviews current literature of what is known on canine semen processing and attempts to standardize techniques to maximize their successful use in clinical settings.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":" 44","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139144360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Staphylococcus pseudintermedius causing presumptive ascending canine abortion 假中间葡萄球菌引起推测性犬上升型流产
Clinical theriogenology Pub Date : 2023-12-29 DOI: 10.58292/ct.v15.9837
Amber Nebel-Karp
{"title":"Staphylococcus pseudintermedius causing presumptive ascending canine abortion","authors":"Amber Nebel-Karp","doi":"10.58292/ct.v15.9837","DOIUrl":"https://doi.org/10.58292/ct.v15.9837","url":null,"abstract":"Despite growing literature regarding evidence of commensal and sometimes pathogenic bacteria in the uterine and vaginal microbiome of dogs, no direct association with reproductive disease has been linked to Staphylococcus pseudintermedius, an opportunistic vaginal commensal (commonly cultured). A multiparous dog had an infectious abortion during late pregnancy over the course of a week. The infectious agent identified on fetal autopsy via culture of the stomach contents and spleen was Staphylococcus pseudintermedius.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139146221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hypothyroidism in canine reproduction 犬类繁殖中的甲状腺功能减退症
Clinical theriogenology Pub Date : 2023-12-27 DOI: 10.58292/ct.v15.9916
Julie Cecere
{"title":"Hypothyroidism in canine reproduction","authors":"Julie Cecere","doi":"10.58292/ct.v15.9916","DOIUrl":"https://doi.org/10.58292/ct.v15.9916","url":null,"abstract":"Hypothyroidism is one of the most commonly diagnosed endocrine diseases in dogs. It has been implicated for many reproductive difficulties in breeding dogs; however, only a few studies have investigated the involvement of thyroid hormones in reproduction. Due to intricacies related to appropriate testing, breeding animals may be placed on thyroid supplementation without justification. The disease, testing, heritability, and animals that may need supplementation are discussed. Additionally, the assumption/appropriateness of breeding animals that are truly hypothyroid is questioned.","PeriodicalId":93421,"journal":{"name":"Clinical theriogenology","volume":"172 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139153739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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