International journal of microbiology and current research最新文献

{"title":"DERMATOPHYTOSIS CAUSED BY Trichophyton verrucosum IN A THREE MONTH OLD CALF IN SHENDAM, PLATEAU STATE, NIGERIA","authors":"J. Dalis, H. Kazeem, Kwaga Jkp, C. Kwanashie, M. Muhammed, P. Okewole, D. Shamaki, M. Ahmed","doi":"10.9735/0975-5276.6.1.515-518","DOIUrl":"https://doi.org/10.9735/0975-5276.6.1.515-518","url":null,"abstract":"A three-month-old calf was seen to have skin lesions involving the head and neck especially around the eyes. The lesions were circular, circumscribed, crusty, grayish-white and perceptibly raised above the skin. Skin scrapings and hair pullouts were aseptically collected and processed for mycology. Direct examination of hair in 20% KOH showed chains of ectothrix spores. Microscopic examination of the iso- late stained with lactophenol cotton blue revealed septate hyphae with numerous clavate microconidia borne laterally from the hyphae and a single longish, thick, smooth- walled, and multi-septated macroconidia with characteristic rat tail (string bean shaped) suggestive of T. verru- cosum. There was no growth on casein vitamin free agar but good growth was observed on medium containing thiamine and inositol. Infor- mation on bovine dermatophytosis from Nigeria is scanty. This finding is significant as it indicates that bovine dermatophytosis may be present in an economically important proportion among cattle especially young animals in Nigeria. The need to carry out more studies on the disease among the cattle population in the country with a view to instituting prevention and control measures was emphasized.","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"28 4","pages":"515-518"},"PeriodicalIF":0.0,"publicationDate":"2014-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91492943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ANTIMICROBIAL SUSCEPTIBILITY OF CRUDE EXTRACTS OF Allium ascalonicum LINN. (WHOLE PLANT) AND Gladiolus psittacinus HOOK (CORM) AGAINST FIVE COMMON PATHOGENS: AN IN VITRO INVESTIGATION","authors":"C. Igbokwe, T. Lawal, Taiye A. Olorunnipa, B. Adeniyi, G. Mahady","doi":"10.9735/0975-5276.6.1.510-514","DOIUrl":"https://doi.org/10.9735/0975-5276.6.1.510-514","url":null,"abstract":"Allium ascalonicum Linn. and Gladiolus psittacinus Hook., are two important plants cultivated across the world for their nutritional as well as medicinal values. In the quest for a more natural bioactive substance to check the rising incidence of antimicrobial resistance amongst pathogens, these two plants were in this study, screened for activity against five common pathogens; namely, Staphylococcus aure- us ATCC 29213, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 35657, and Candida albicans ATCC 90029. Phytochemical screening carried out on the extracts showed the presence of secondary metabolites like saponins, cardiac glycoside, alkaloids and flavonoids in both plant samples but only Allium ascalonicum yielded essential oil, while carbohydrate and tannins were detected in Gladiolus psittacinus. While the crude extracts of Gladiolus psittacinus did not show any inhibitory potential against any of the tested organisms, three pathogens were susceptible to Allium ascalonicum, with minimum inhibitory concentration (MIC) of 100 mg/mL. Bactericidal kinetics studies on the three pathogens showed that within the exposure time of 4 hours, the organisms were killed (69% and 95% at 2 × MIC and 4 × MIC, respectively) , a result which compared well with the positive control Gentamicin. The result of this study while agreeing with earlier reports on the non-activity of Gladiolus corms against Candida albicans contradicts the same report on the activity of the crude extract of this plant against Pseudomonas aeruginosa. The significant inhibitory potential of the crude methanolic extract of Alli- um ascalonicum against food borne pathogens such as Staphylococcus aureus, Escherichia coli and nosocomial pathogen such as Klebsiella pneumoniae, suggests that it can be explored as a natural alternative to chemical preservatives in food industries, and in the formulation of drugs against nosocomial infections .","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"40 1","pages":"510-514"},"PeriodicalIF":0.0,"publicationDate":"2014-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87094462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CROSS-RESISTANCE OF AZOLES, ECHINOCANDINS, FLUCYTOSINE AND AMPHOTERICIN B IN CLINICALLY IMPORTANT HYPHOMYCETES","authors":"A. Schmalreck, W. Fegeler, K. Becker, C. Lass‐Flörl, Czaika","doi":"10.9735/0975-5276.6.1.519-544","DOIUrl":"https://doi.org/10.9735/0975-5276.6.1.519-544","url":null,"abstract":"The existing standardized micro-broth dilution methods for in vitro testing of moulds (CLSI, EUCAST) are referred to as reference methods and therefore not intended for routine testing. They are time-consuming and dependent on sporulating hyphomycetes. In this study a new, time saving and easy-to-perform method for inoculum preparation for routine susceptibility testing has been applied. It is independent of spore production and proofed to produce comparable results to the conidia based methods, indicating that it can be used for all types of hyphae-and/or conidia-forming fungi. The MICs of amphotericin B, flucytosine, fluconazole, posaconazole, voriconazole, anidulafungin, caspofungin and micafungin of 198 moulds were determined with two different culture media (YST and RPMI 1640) according to the DIN microdilution assay, and compared to appropri- ate international studies. The \"new\" inocula with YST (DIN) and RPMI 1640 (EUCAST) medium showed similar MIC distributions for all moulds tested to the conidia method, with more than 92% of the MICs read at 24 h and 48 h within ± 1log2-dilution. Although azoles, flucyto- sine and amphotericin B showed comparable results, differences in echinocandin endpoints between different multicentre studies were de- termined. According to the literature, the minimum effective concentration (MEC) should be equivalent to the minimum inhibitory concentra- tion (MIC). However, due to the encountered bias in echinocandin-endpoint determinations this has to be questioned. Evaluation of cross- resistance demonstrated that no individual strain out of 198 was in parallel susceptible to all eight antifungal agents tested. Cross-resistance between azoles, echinocandins, amphotericin B, and flucytosine could be detected quantitatively with a new method for fungi. It ranges from two to sevenfold, and demonstrates quantitatively different and species-specific susceptibility/resistance patterns.","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"51 1","pages":"519-544"},"PeriodicalIF":0.0,"publicationDate":"2014-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80797676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MOBILE PHONE CAN TRANSMIT MORE THAN JUST A CALL- A MODE OF NOSOCOMIAL TRANSMISSION","authors":"Shah Pd, Trivedi Na, P. Geetha, Trivedi Mb, S. M. Murawala, Soni St, Vegad Mm","doi":"10.9735/0975-5276.5.7.502-505","DOIUrl":"https://doi.org/10.9735/0975-5276.5.7.502-505","url":null,"abstract":"Background: Nosocomial infections continue to pose increased mortality and morbidity in patients. The mobile phone and hands of health professionals may harbor many harmful pathogens which can serve as reservoir of infections. Objective: In this study, we investigated the rate of microbial contamination of mobile phones and hands of health professionals in our tertiary care hospital. Methods: In this study we took 320 samples from different wards, ICUs and OT staff. The samples were taken from 2 groups i.e. doctors and nursing staff. Swabs were taken from dominant hands and mobile phones. Culture and antibiotic susceptibility was carried out as per standard microbiological procedures. Results: From 160 samples of mobile phones and hands each, 70.62% and 64.37% of swabs demonstrated evidence of microbial contamination with different type of organisms, respectively. Most commonly found organisms were coagulase negative staphylococci (46.7%). Established nosocomial pathogens like Staphylococcus aureus, Escherichia coli, Klebsiella spp. & enterococci, were isolated from 56.6% of mobile and 42.7% of hand samples. Methicillin resistance was seen in 25.5% of staphylococcal isolates. The colonization of isolates in hands was almost similar to that in mobile phones.","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"4 1","pages":"502-505"},"PeriodicalIF":0.0,"publicationDate":"2013-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91308369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MOLECULAR DETECTION OF AmpC β-LACTAMASE GENE IN Morganella morganii CLINICAL ISOLATES","authors":"Al-Muhanna As","doi":"10.9735/0975-5276.5.7.506-509","DOIUrl":"https://doi.org/10.9735/0975-5276.5.7.506-509","url":null,"abstract":"","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"50 1","pages":"506-509"},"PeriodicalIF":0.0,"publicationDate":"2013-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88787424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MICROBIOLOGICAL STUDY OF VARIOUS CANDIDA SPECIES AND ITS ANTIFUNGAL SENSITIVITY TESTING ISOLATED FROM ANTENATAL WOMEN WITH VAGINITIS, IN TERTIARY CARE TEACHING HOSPITAL, WESTERN INDIA","authors":"Pan Pa, K. Rk, Mehta Rc, S. Soni, A. Nanera, Trivedi Na, Desai Kj, Vegad Mm","doi":"10.9735/0975-5276.5.6.486-489","DOIUrl":"https://doi.org/10.9735/0975-5276.5.6.486-489","url":null,"abstract":"","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"16 1","pages":"486-489"},"PeriodicalIF":0.0,"publicationDate":"2013-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89056634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"STUDIES ON DRUG-RESISTANCE PATTERN BY PHENOTYPIC METHODS IN Mycobacterium tuberculosis ISOLATES IN A TERTIARY CARE HOSPITAL","authors":"Patil Sd, Angadi Km, Modak Ms, Bodhankar Mg","doi":"10.9735/0975-5276.5.6.497-501","DOIUrl":"https://doi.org/10.9735/0975-5276.5.6.497-501","url":null,"abstract":"","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"63 1","pages":"497-501"},"PeriodicalIF":0.0,"publicationDate":"2013-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82787441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IMMUNIZATION OF MICE WITH KILLED E. coli K99 VACCINE FOR PROTECTION AGAINST COLIBACCILLOSIS","authors":"Yousif Aa, Mahmood Nm, Al-Taai Na","doi":"10.9735/0975-5276.5.6.482-485","DOIUrl":"https://doi.org/10.9735/0975-5276.5.6.482-485","url":null,"abstract":"Enterotoxigenic E. coli is the most common enteropathogen that causes diarrhea in newborn farm animals. The bacteria cause diarrhea by adhering to colonizing and producing enterotoxins in the small intestine they are not invasive [2]. Enterotoxigenic E. coli (ETEC) strains as the important cause of sever diarrhea in newborn calves carry fimbriae (such as F5 (K99) and F41) mediating binding of the bacteria to microvilli of enterocytes of the small intestine [3,4]. Osman, et al [5] report that the large and small ruminants could be a potential source of infection with enterovirulent Escherichia coli pathotypes in humans in Egypt.","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"2 1","pages":"482-485"},"PeriodicalIF":0.0,"publicationDate":"2013-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89577548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EFFECTIVENESS OF CHROM AGAR CANDIDA, A DIFFERENTIAL ISOLATION MEDIUM FOR RAPID IDENTIFICATION OF CLINICALLY IMPORTANT CANDIDA SPECIES","authors":"G. Gahlot, S. Soni, P. Gandhi, Sachin M Patel, K. Rk, Vegad Mm, Savalia Aa","doi":"10.9735/0975-5276.5.6.494-496","DOIUrl":"https://doi.org/10.9735/0975-5276.5.6.494-496","url":null,"abstract":"","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"23 1","pages":"494-496"},"PeriodicalIF":0.0,"publicationDate":"2013-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78244334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"INDUCTION OF TEMPERATE CYANOPHAGES USING HEAVY METAL - COPPER","authors":"Marei Em, Elbaz Rm, H. Amm","doi":"10.9735/0975-5276.5.5.472-475","DOIUrl":"https://doi.org/10.9735/0975-5276.5.5.472-475","url":null,"abstract":"","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"22 1","pages":"472-475"},"PeriodicalIF":0.0,"publicationDate":"2013-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80011782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}