{"title":"OCCURRENCE AND ANTIMICROBIAL RESISTANCE OF Salmonella spp. AND OTHER ENTEROBACTERIA RECOVERED FROM KITCHEN EQUIPMENT OF A UNIVERSITY HOSPITAL IN RIO DE JANEIRO, BRAZIL","authors":"Miyahira Rf, Santos Efas, Freitas As, Queiroz Mlp","doi":"10.9735/0975-5276.5.5.467-471","DOIUrl":"https://doi.org/10.9735/0975-5276.5.5.467-471","url":null,"abstract":"","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"55 1","pages":"467-471"},"PeriodicalIF":0.0,"publicationDate":"2013-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73287316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Camele, Emanuela Ventrella, L. Scrano, Stella Lovelli, Mariana Amato, A. Lucano
{"title":"USE OF PLANT GROWTH PROMOTING BACTERIA (PGDB) FOR PROMOTING TOMATO GROWTH AND ITS EVALUATION AS BIOLOGICAL CONTROL AGENT","authors":"I. Camele, Emanuela Ventrella, L. Scrano, Stella Lovelli, Mariana Amato, A. Lucano","doi":"10.9735/0975-5276.5.5.452-457","DOIUrl":"https://doi.org/10.9735/0975-5276.5.5.452-457","url":null,"abstract":"The plant growth promoting effect of some bacteria usable to control plant diseases has a potential for resolving technical problems of plant production. The effects of Burkholderia gladioli pv. agaricicola ICMP11096 on development of tomato seedlings development and control of their infection by Fusarium oxysporum and Sclerotinia sclerotiorum were evaluated in the present study. Bacterized plants showed a higher shoot and root weight and a more extended root system in terms of total length and root density than non-bacterized plants. The bacter- izing treatments gave rise to significant reduction of disease symptoms such as leaf wilting induced by F. oxysporum infection and/or root ne- crosis and leaf yellowing by S. sclerotiorum. The potential use of Burkholderia gladioli pv. agaricicola ICMP11096 in biocontrol of fungal patho- gens and to enhance seedling growth parameters is underlined as a tool to achieve a high commercial quality of nursery tomato plants. Keywords- Plant growth promoting bacteria, Burkholderia gladioli pv. agaricicola, Fusarium oxysporum, Sclerotinia sclerotiorum, tomato, bio- logical control","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"74 1","pages":"452-457"},"PeriodicalIF":0.0,"publicationDate":"2013-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74271143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Phyto-Microbial Degradation of Glyphosate in Riyadh Area","authors":"A. Abdel-Megeed, S. Mw, A. Ho, H. Ali","doi":"10.9735/0975-5276.5.5.458-466","DOIUrl":"https://doi.org/10.9735/0975-5276.5.5.458-466","url":null,"abstract":"Green house studies were conducted to determine the ability of plant Amaranth, Amaranthus caudate and two isolated bacterial strains from rhizosphere region for cleaning up glyphosate residues in soil and plants. The analytical study of the degradation of glyphosate was carried out in the laboratory conditions. Amaranth, Amaranthus caudate and two isolated bacterial strains namely Pseudomonas aeruginosa and Bacillus megaterium could degrade glyphosate in 5 days. These results suggested that phytoremediation could accelerate the degradation of glyphosate residues in plants and in rhizosphere region as well. Glyphosate had strong effect on bacterial DNA where many DNA bands were affected. This could be explained that the effect of herbicide glyphosate on the protein profile may reflex somehow DNA mutation occurred during the assimilation of those toxic compounds. Therefore, the alteration occurred in both DNA and protein profiles is considered a degree of tolerance that lead to DNA mutation to cope with the assimilation of this compound. Therefore, the phytoremediation way could be a promising tool in program is to protect public health and the environment by ensuring the safety and availability of herbicides and pesticide alternatives.","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"31 1","pages":"458-466"},"PeriodicalIF":0.0,"publicationDate":"2013-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76608355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"PROBIOTIC CARRIER POTENTIAL, SENSORY PROPERTIES AND MICROBIAL QUALITY OF UGBA (PENTACLETHRA MACROPHYLLA) AND OGIRI (RICINUS COMMUNIS)","authors":"S. Oranusi, W. Braide, E. Chinakwe","doi":"10.9735/0975-5276.5.5.476-481","DOIUrl":"https://doi.org/10.9735/0975-5276.5.5.476-481","url":null,"abstract":"Ugba’ and ‘Ogiri’, fermented products of African oil bean (Pentaclethra macrophylla) and Castor oil seed (Ricinus communis), were screened for microbiological profile and ability to serve as carrier medium for probiotic, the sensory acceptability of products over twenty one days storage was also analyzed. Assay of unpasteurized products from different food vendors gave mean heterotrophic bacterial count for Ugba ranging from 6.20 x1010-2.96x1011 cfu/g, bacteria count in Ogiri samples range from 5.10x1010-1.21x1011 cfu/g. Mean fungal count in Ugba samples range from 8.0x108-2.01x1010 cfu/g while count of fungi in Ogiri range from 2.1x109-1.21x1010 cfu/g. The bacteria isolated in-clude species of Bacillus, Staphylococcus, Enterococcus, Serratia, Klebsiella, Micrococcus, Pseudomonas, Streptococcus and Lactobacillus. The fungi species implicated include Rhizopus, Geotrichum, Saccharomyces, Mucor, Penicillium, Fusarium and Aspergillus. Microbial load of inoculated LAB (LASO and LAWB) multiplied in all the products throughout the storage period, there was also increase in number of recov-ered LAB from the stool samples through the period of study. Unpasteurized products of both Ugba and Ogiri declined in sensory attributes after day 5-9 while Ogiri and Ugba samples with LASO and LASO+LAWB combinations had better sensory properties at day 21. Ogiri and Ugba could serve as good carrier medium for probiotic with good organoleptic properties, however texture stability of these products during storage need further investigation. Hazard Analysis Critical Control Point assessment, Good Manufacturing Practice and packaging are nec-essary to curtail microbial contaminations in these products.","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"63 1","pages":"476-481"},"PeriodicalIF":0.0,"publicationDate":"2013-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89501950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Y. Suzuki, Juliana Kina, Mônica Kina, Eunice Fumico Umeda Kina, J. Kina
{"title":"Reparative phase events on periodontal disease progression: interpretation and considerations","authors":"T. Y. Suzuki, Juliana Kina, Mônica Kina, Eunice Fumico Umeda Kina, J. Kina","doi":"10.9735/0975-5276.5.4.439-444","DOIUrl":"https://doi.org/10.9735/0975-5276.5.4.439-444","url":null,"abstract":"1Department of Surgery and Integrated Clinic, Araçatuba School of Dentistry, São Paulo State University UNESP, Araçatuba, SP, Brazil. 2Department of Dental Materials and Prosthodontics, Araçatuba School of Dentistry, São Paulo State UniversityUNESP, Araçatuba, SP, Brazil. 3Department of Pediatric Dentistry and Public Health, Araçatuba School of Dentistry, São Paulo State UniversityUNESP, Araçatuba, SP, Brazil. 4Private Practice, Araçatuba, SP, Brazil. *Corresponding Author: Emailkinajr@hotmail.com","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"40 1","pages":"439-444"},"PeriodicalIF":0.0,"publicationDate":"2013-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73187890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"HIGH RISK HPV GENOTYPING AMONG PRIMARY INFERTILE WOMEN","authors":"Shanware As, P. Patil, Shegokar","doi":"10.9735/0975-5276.5.4.435-438","DOIUrl":"https://doi.org/10.9735/0975-5276.5.4.435-438","url":null,"abstract":"Human Papilloma virus (HPV) infection is common among young, sexually active women. Human Papilloma viruses are non enveloped, double stranded DNA virus of approximately 8000 base pairs. The viral genome consists of early & late regions containing open reading frames (ORFs), resulting in translation of functional proteins. The two late region open reading frames (ORFs), L1 & L2 encode two structural proteins composing the major and minor capsid proteins, respectively about 15% of the viral genome, non encoding for proteins, plays a role in regulation of transcription &viral DNA replication. The early regions ORFs (E1, E2, E4,E7) are expressed early in the viral life cycle. Three of the early ORFs are the oncogenes E5, E6 & E7. Modulating the transformation process. two regulatory proteins E1 & E-, modulate replication of transcription [1]. The HPV family consists of 120 types, of which 40 different types can infect the anogenital mucosa [2,3]. Several HPV high risk types such as 16, 18, 31, 33, 35 & 45 have been implicated with an etiological role in cervical carcinogenesis [4].","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"102 1","pages":"435-438"},"PeriodicalIF":0.0,"publicationDate":"2013-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75857012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"SEROPREVALENCE OF HEPATITIS B SURFACE ANTIGEN AMONG SOUTH-WESTERN NIGERIAN UNDERGRADUATES","authors":"A. Jemilohun, B. Oyelade, S. Oiwoh","doi":"10.9735/0975-5276.5.4.445-447","DOIUrl":"https://doi.org/10.9735/0975-5276.5.4.445-447","url":null,"abstract":"","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"134 1","pages":"445-447"},"PeriodicalIF":0.0,"publicationDate":"2013-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89288811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Debnath, Shauroseni Palchoudhuri, N. Chatterjee, D. Sinharoy, S. Bhowmick, T. Pal, Sukhen Das, S. Dastidar
{"title":"EXPERIMENTAL EVALUATION OF SYNERGISTIC ACTION BETWEEN ANTIBIOTICS AND THE ANTIPSYCHOTIC ANTIMICROBIAL TRIFLUPROMAZINE","authors":"S. Debnath, Shauroseni Palchoudhuri, N. Chatterjee, D. Sinharoy, S. Bhowmick, T. Pal, Sukhen Das, S. Dastidar","doi":"10.9735/0975-5276.5.4.430-434","DOIUrl":"https://doi.org/10.9735/0975-5276.5.4.430-434","url":null,"abstract":"Significant antimicrobial action of the antipsychotic drug triflupromazine (Tp) against various genera of bacteria has been evaluated extensively in previous studies. This present investigation was designed to study whether this phenothiazine is able to augment action of an antibiotic when tested in combination. A total of twelve different bacterial strains belonging to various genera were used and tested to be sen- sitive against many antibiotics and the non antibiotic Tp. The Minimum inhibitory concentration (MIC) of all the test bacteria with respect to the antibiotics ranged fron 2- 50 µg/ml. In case of Tp, MIC ranged from 25-200 µg/ml. Disc diffusion assays revealed synergism between Tp and penicillin, ampicillin, carbenicillin, streptomycin (Sm), gentamicin and ciprofloxacin. Antagonistic effect was shown between Tp and cloxacillin, erythromycin and tetracyclin. Most effective and statistically significant (p<0.001) synergism was observed when Tp was combined with Sm. Following checkerboard method, the Fractional inhibitory concentration (FIC) index of the duo was determined to be 0.375, which confirmed significant synergism. The pair when subjected to in vivo experiments in mice challenged with Salmonella enterica serovar Typhimurium NCTC 74, showed statistically significant (p<0.001) mouse protection and also resulted in reduction of the infection in internal organs. This further suggests the pair to be highly synergistic. Thus, from this study it can be concluded that the antimicrobial activity of the non antibiotic Tp can be further remarkably increased in combination with a suitable antibiotic to combat against multi drug resistant bacteria and can be used effectively as an alternative therapy.","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"57 1","pages":"430-434"},"PeriodicalIF":0.0,"publicationDate":"2013-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87377537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Akujobi, C. C. Ezeanya, K. M. Emeka-Okafor, J. Ebenebe
{"title":"A STUDY ON SIGNIFICANT BACTERIURIA AMONG CHILDREN ATTENDING THE OUT-PATIENT CLINIC OF A UNIVERSITY TEACHING HOSPITAL, NIGERIA","authors":"C. Akujobi, C. C. Ezeanya, K. M. Emeka-Okafor, J. Ebenebe","doi":"10.9735/0975-5276.5.4.448-451","DOIUrl":"https://doi.org/10.9735/0975-5276.5.4.448-451","url":null,"abstract":"","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"102 1","pages":"448-451"},"PeriodicalIF":0.0,"publicationDate":"2013-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88029056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"MEDIUM OPTIMIZATION OF Burkholderia gladioli pv. agaricicola ENHANCED THE PRODUCTION OF ANTIMICROBIAL SUBSTANCES","authors":"H. S. Elshafie, I. Camele, S. Bufo","doi":"10.9735/0975-5276.5.3.399-403","DOIUrl":"https://doi.org/10.9735/0975-5276.5.3.399-403","url":null,"abstract":"-Numerous species in the genus Burkholderia have interesting properties for potential industrial applications including production of antibiotics, biosurfactants, bioplastics and degradation of environmental contaminants. The aim of this research was study the effect of different carbon and nitrogen sources on Burkholderia gladioli pv. agaricicola strain ICMP11096 growth and bioactivity of produced secondary metabolites against gram-positive bacterium Bacillus megaterium and plant pathogenic fungi Rhizoctonia solani . The additional carbon sources were sucrose, fructose and lactose whereas, the additional nitrogen sources were, urea, potassium nitrate and ammonium nitrate. The results demonstrated that the addition of new carbon and nitrogen sources to the minimal mineral nutrient media lead to decreasing the growth rate of studied bacterial strain and increasing the production of bioactive substances. The maximum production of bioactive substances of studied bacterial strain was obtained using ammonium nitrate and lactose. The purified filtrate of the studied bacterial strain was fractionated by High Performance Liquid Chromatography (HPLC) and the antimicrobial activity of five isolated single peaks was evaluated against gram positive bacteria B. megaterium ITM100 and gram negative bacteria Escherichia coli ITM103. The most bioactive peak was number two with 12800 and 6400 Ua.ml -1 against B. megaterium and E. coli , respectively. The obtained results suggested that the nutrient amendments can increase the production of antimicrobial substances and this may be a useful strategy for improving the biocontrol efficiency.","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"19 1","pages":"399-403"},"PeriodicalIF":0.0,"publicationDate":"2013-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83351203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}