HIGH RISK HPV GENOTYPING AMONG PRIMARY INFERTILE WOMEN

Abstract

Human Papilloma virus (HPV) infection is common among young, sexually active women. Human Papilloma viruses are non enveloped, double stranded DNA virus of approximately 8000 base pairs. The viral genome consists of early & late regions containing open reading frames (ORFs), resulting in translation of functional proteins. The two late region open reading frames (ORFs), L1 & L2 encode two structural proteins composing the major and minor capsid proteins, respectively about 15% of the viral genome, non encoding for proteins, plays a role in regulation of transcription &viral DNA replication. The early regions ORFs (E1, E2, E4,E7) are expressed early in the viral life cycle. Three of the early ORFs are the oncogenes E5, E6 & E7. Modulating the transformation process. two regulatory proteins E1 & E-, modulate replication of transcription [1]. The HPV family consists of 120 types, of which 40 different types can infect the anogenital mucosa [2,3]. Several HPV high risk types such as 16, 18, 31, 33, 35 & 45 have been implicated with an etiological role in cervical carcinogenesis [4].