International journal of microbiology and current research最新文献

{"title":"STUDY ON GENERATION MEAN ANALYSIS FOR SEED YIELD AND ITS ASSOCIATED TRAITS IN LINSEED (Linum usitatissimum L.)","authors":"P. Yadav, R. Yadav, S. Dubey, Harpinder Singh, Vishwanath, Y. Pandey, Sanjeev Kumar","doi":"10.9735/0975-5276.10.3.1027-1034","DOIUrl":"https://doi.org/10.9735/0975-5276.10.3.1027-1034","url":null,"abstract":"","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"87 1","pages":"1027"},"PeriodicalIF":0.0,"publicationDate":"2018-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78296059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SCREENING OF POTENTIAL MICROALGAE SPECIES FROM DIFFERENT NATURAL ENVIRONMENT FOR BIODIESEL PRODUCTION","authors":"Harinikumar Kodihalli Machegowda, Sriappareddy Tamulpaudi, Manoj Kumar Haniyambadi Basavegowda, Purushothaman Yelagiri Munisamy","doi":"10.9735/0975-5276.10.3.1052-1057","DOIUrl":"https://doi.org/10.9735/0975-5276.10.3.1052-1057","url":null,"abstract":"Microalgal samples were collected from different regions of India. The samples thus obtained were serially diluted, colonies were further purified and identified as Desmodesmus abundans, Scendesmus pectinatus, Tetranephris brasiliensis, Desmodesmus armatus, Scendesmus costato-granulatus, Neocystis brevis, Chlorella sorokiniana, Scendesmus acutus, Pseudomuriella species and Chlorella minutissima by 18s rRNA sequencing. The effect of growth conditions was studied under different media such as Bold Basal Media (BBM), Blue Green 11 media (BG11) and Tris-Acetate Phosphate media (TAP). The highest growth rate was occurred in Desmodesmus abundans on 19th day in TAP media whereas the lowest growth rate was occurred in Tetranephris brasiliensis in BBM media. Under similar environmental conditions average specific wet and dry biomass were found maximum in Chlorella minutissima, Desmodesmus abundans and Scendesmus costatogranulatus in TAP media when compared to BBM and BG11. The highest amount of lipid was obtained in Desmodesmus armatus (43.7%) grown in TAP media. Nile red staining and thin layer chromatography confirms the accumulation of total lipids. The reaction condition with that of n -hexane to methanol ratio 1:2, solvent dosage 5.0 ml, reaction temperature 90°C, reaction time 2.0 h and catalyst volume 0.5 ml of the direct transesterification can reach the conversion yield of 90.23%, 89.25% and 86.65% found in Desmodesmus abundans, Desmodesmus armatus and Scendesmus costato-granulatus respectively. There is a prospective in algal biotechnology by exploitation of different media composition for the growth formulation in microalgae species which is inexpensive for biomass cultivation in large scale production of lipids that can be harnessed for biodiesel production. KeywordsMicroalgae, Biomass, Lipids, Transesterification and Biodiesel","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"79 1","pages":"1052"},"PeriodicalIF":0.0,"publicationDate":"2018-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80109913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IDENTIFICATION AND CHARACTERIZATION OF CAUSAL AGENT OF CERCOSPORA LEAF SPOT DISEASE OF OKRA","authors":"S. Hasan, Md. Firose Hossain, Zannati Ferdous Zaoti, Md Saroar Jahan, Md. Faruk Hasan, Md Asadul Islam, B. Sikdar","doi":"10.9735/0975-5276.10.3.1015-1019","DOIUrl":"https://doi.org/10.9735/0975-5276.10.3.1015-1019","url":null,"abstract":"","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"101 9","pages":"1015"},"PeriodicalIF":0.0,"publicationDate":"2018-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91549844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EFFECT OF COW URINE (GOMUTRA) AS A SOURCE OF NITROGEN ON GROWTH, YIELD AND NITROGEN UPTAKE IN RICE (Oryza sativa L.)","authors":"S. Singh, K. Maurya, G. Singh","doi":"10.9735/0975-5276.10.3.1035-1037","DOIUrl":"https://doi.org/10.9735/0975-5276.10.3.1035-1037","url":null,"abstract":"Rice (Oryza Sativa L.) is an important food crop. The demand for rice continues to increase owing to continued growth of population. It is predi cted that a 50% to 60% increase in rice production will be required to meet demand by 2025 in India. Cow urine (Gomutra) is used for several centuries in different ways in many parts of the world. Cow urine contains nitrogen constituents in different amounts and may be used as source of nitrogen for p lant nutrition. Hence, the present study was taken up to evaluate the effect of cow urine on growth yield and nutrient uptake of rice. Accordingly, a field experiment was conducted during kharif 2017at crop cafeteria of BHUKrishi Vigyan Kendra, Institute of Agricultural Sciences, Rajiv Gandhi South campus, Barkachha, Mirzapur (Banaras Hindu University) in randomized block design taking rice variety Shusk Samrat as a test crop. The soil of the study region was red soil having pH 7.8, EC 0.6 7 dS m-1, organic carbon 0.42%, available N 185.3, P 9.6, K 189 and S 9.4 kg ha-1and available micronutrient Fe 32.6, Mn 18.6, Zn 2.26, Cu 2.1 and Boron 0.98 mg kg -1. The experiment consisted of five treatments comprising T1 (controlno input), T2 (N at 120 kg ha-1), T3 (N at 150 kg ha-1), T4 (N at 120 kg ha-1 + cow urine) and T5 (N at 100 kg ha-1) taking four replications in randomized block design. Cow urine procured from same cow was applied weekly up to six weeks from the date of transplanting. The results of the study revealed that paddy crop favourably responded to different levels of nitrogen through fertilizer alone and in combination of cow urine. Plant growth (plant height, number of tiller) and yield (grain and straw) significantly increased as compared to control by the application of nitrogen. Applica tion of N at 120 kg ha-1 + cow urine registered maximum dry matter production. Without cow urine, application of nitrogen at 150 kg ha -1 recorded higher yield than control and 120 kg ha-1 level. Nitrogen content in plant (grain and straw) and uptake were also found maximum when nitrogen was applied at 120 kg ha -1 + cow urine. Key wordsRice, Cow urine","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"1 1","pages":"1035"},"PeriodicalIF":0.0,"publicationDate":"2018-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83039828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Moellerella wisconsensis ISOLATED FROM STOOL SAMPLE HAVING PROLONGED HISTORY OF DIARRHEA","authors":"Anurag D. Zaveri, Vinay Bhomia, Dilip N. Zaveri, B. Lakshmi","doi":"10.9735/0975-5276.10.2.1009-1010","DOIUrl":"https://doi.org/10.9735/0975-5276.10.2.1009-1010","url":null,"abstract":"Previously called as Enteric Group [1], Moellerella wisconsensis is a Gram Negative bacilli from Enterobacteriaceae family named for the first time in 1984 by Hickman Brenner et al isolated from patients with diarrhea is very rarely reported organism. Its isolation from various clini cal specimens have been reported such as gall bladder tissue, blood culture and stool. Moellerella wisconsensis has very few or less case reports reported because it has been recovered from various non clinical sources like water, food and sometimes from the animal origin. We are reporting a case of i solation of Moellerella wisconsensis from stool sample for the first time in western region of India (Ahmedabad-Gujarat). KeywordsMoellerella wisconsensis, Diarrhea, Stool, Enteric Bacteria, Pathogen.","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"10 1","pages":"1009"},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82267781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EVALUATION OF MONOCROTOPHOS DEGRADATION BY Lysinibacillus sphaericus JX012235 ISOLATED FROM SWINE WASTE","authors":"Sundaram Meignanalakshmi, J. Deepika, M. Charulatha, K. Vijayarani","doi":"10.9735/0975-5276.10.2.1011-1014","DOIUrl":"https://doi.org/10.9735/0975-5276.10.2.1011-1014","url":null,"abstract":"In India, the mainly used pesticide in agricultural crops is Monocrotophos, an organophosphorus pest icide. It is denoted as extremely toxic and their exposure to environment is considered to be lethal. In this study, degradation of Monocrotophos by Lysinibacillus sphaericus JX012235 a bacterium isolated from swine waste was studied. Growth studies of the bacteria in mineral salt broth supplemented with the pesticide at the concentration of 100-1000 ppm initially confirmed the degradation of the pesticide. The bacterium was found to survive when pesticide was given as a sole source of nutrition. The pesticide degradation was confirmed by LC-MS. The phosphatase activity of bacterium was found to be 69U/ml. KeywordsMonocrotophos, Lysinibacillus sphaericus, Biodegradation, Swine waste, Phosphatase activity.","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"59 1","pages":"1011"},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72752085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PREVALENCE AND GENOTYPE DIVERSITY OF ANAL HPV INFECTION AMONG HIV-INFECTED WOMEN IN BELEM, PARA, NORTH OF BRAZIL","authors":"L. Rodrigues, R. Ishak, A. Vallinoto, R. N. M. Feitosa, V. Azevedo, R. V. Silvestre, R. Laurentino, J. Monteiro, A. R. Silva, L. Machado","doi":"10.9735/0975-5276.10.1.993-996","DOIUrl":"https://doi.org/10.9735/0975-5276.10.1.993-996","url":null,"abstract":"The high prevalence and diversity of anal Human Papillomavirus (HPV) infection have been observed in women infected with the human immunodeficiency virus (HIV) and little is known about the epidemiology of anal HPV infection among HIV-infected women in North Region of Brazil. The objective of this cross-sectional study was to determine the prevalence of anal HPV infection, genotypes diversity and its relationship with demographic aspects of HIV-infected attending in Belém, Pará, Brazil. A questionnaire regarding socio-demographic variables and sexual behavior was used and anal samples were collected from 108 women recruited from a HIV counselling and testing in the Belém, Pará, an important metropolis of the northern region of Brazil. A blood sample was also obtained to determine CD4+ T-cell counts and viral load levels of HIV. HPV DNA was detected by PCR amplification of a conserved region of the HPV L1 gene and further genotyped with direct DNA sequencing. HPV infection was detected in 29 (26,8%) women and HPV types were identified in 20 samples. Twelve different types were found (HPV-16, 58, 59, 6, 11, 53, 61, 62, 66, 70, 71, 102) and low risk types were present in 85% of samples. Time of early first intercourse was associated were associated with HPV infection. In conclusion we found a low prevalence of HPV infection and low risk types in this cohort and we emphasize the relevance of implementation of screening program for anal cancer and HPV infection in HIV-infected women population.","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"50 1","pages":"993"},"PeriodicalIF":0.0,"publicationDate":"2018-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90291427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SEROPREVALENCE OF HEPATITIS C VIRUS INFECTION AMONG PREGNANT WOMEN ATTENDING A TEACHING HOSPITAL OF ATARIA, NORTH INDIA","authors":"R. Khatoon, N. Jahan","doi":"10.9735/0975-5276.10.1.1001-1004","DOIUrl":"https://doi.org/10.9735/0975-5276.10.1.1001-1004","url":null,"abstract":"-Hepatitis C virus (HCV) is a major cause of viral hepatitis and hepatic dysfunction during pregnancy throughout the world. HCV infection leads to complications like premature labour, preterm delivery and maternal mortality. Virus may be transmitted vertically to neonates leading to neonatal hepatitis. The present study was done to estimate the seroprevalence of Hepatitis C virus infection among pregnant women. Total of 2067 pregnant women were enrolled in the study whose serum sample was tested for anti-Hepatitis C virus antibody using rapid test and those found reactive were confirmed using enzyme linked immunosorbent assay (ELISA). Out of 2067 pregnant women tested, 24 were found to be reactive by both rapid test and ELISA, hence the seroprevalence was 1.2%. Maximum seropositivity was seen in pregnant women of age group 26-30 years. Seropositivity was more among working pregnant women, belonging to rural areas and those who were poor and belonged to lower socio-economic class. All these findings were statistically significant. Amongst the risk factors studied, most of the seropositives gave history of previous surgery followed by history of dilatation and curettage. To conclude, it is strongly recommended that all pregnant women should be screened for anti-Hepatitis C virus antibodies in order to know their infection status and thus prevent adverse outcome of pregnancy and its vertical transmission to their neonates.","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"88 1","pages":"1001"},"PeriodicalIF":0.0,"publicationDate":"2018-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82532857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PHENOTYPIC DETECTION OF AmpC β-LACTAMASE PRODUCTION IN GRAM NEGATIVE CLINICAL ISOLATES","authors":"Neetu Gupta, Savita V Jadhav, N. Gandham, R. Misra, A. Varma","doi":"10.9735/0975-5276.10.1.997-1000","DOIUrl":"https://doi.org/10.9735/0975-5276.10.1.997-1000","url":null,"abstract":"","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"22 1","pages":"997"},"PeriodicalIF":0.0,"publicationDate":"2018-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87738056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GROWTH DYNAMICS OF Pseudomonas putida (NBAII-RPF9) UNDER ABIOTIC STRESS CONDITIONS","authors":"K. Ashwitha, R. Rangeshwaran","doi":"10.9735/0975-5276.10.1.1005-1008","DOIUrl":"https://doi.org/10.9735/0975-5276.10.1.1005-1008","url":null,"abstract":"Experiments were conducted to know the growth variations adopted by Pseudomonas putida (NBAII-RPF 9) an abiotic stress tolerant plant growth promoter and biological control agent. Cell growth and survival was monitored under different time periods with two temperature regi mes (25 ̊ and 45o C) and data on viable cell count was analysed. P. putida (NBAII-RPF9) experienced a delayed lag phase when grown under heat stress as compared to its growth at normal temperature which was complemented by expression of higher whole cell protein concentration. Under heat stress increased protein content was noticed in lag phase. Whole cell protein analysis of P. putida (NBAII-RPF 9) by SDS-PAGE analysis showed expression of proteins ranging between 40KDa to 60KDa which match with molecular weight of major heat stress related proteins like GroEL and DnaK. This supported the fact that various proteins were triggered under st ress conditions to ensure its survival. KeywordsPseudomonas putida, heat stress, salt stress, growth curve, exponential phase, protein profile, HSPs, antagonism.","PeriodicalId":92914,"journal":{"name":"International journal of microbiology and current research","volume":"9 1","pages":"1005"},"PeriodicalIF":0.0,"publicationDate":"2018-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90707482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}