Sundaram Meignanalakshmi, J. Deepika, M. Charulatha, K. Vijayarani
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EVALUATION OF MONOCROTOPHOS DEGRADATION BY Lysinibacillus sphaericus JX012235 ISOLATED FROM SWINE WASTE
In India, the mainly used pesticide in agricultural crops is Monocrotophos, an organophosphorus pest icide. It is denoted as extremely toxic and their exposure to environment is considered to be lethal. In this study, degradation of Monocrotophos by Lysinibacillus sphaericus JX012235 a bacterium isolated from swine waste was studied. Growth studies of the bacteria in mineral salt broth supplemented with the pesticide at the concentration of 100-1000 ppm initially confirmed the degradation of the pesticide. The bacterium was found to survive when pesticide was given as a sole source of nutrition. The pesticide degradation was confirmed by LC-MS. The phosphatase activity of bacterium was found to be 69U/ml. KeywordsMonocrotophos, Lysinibacillus sphaericus, Biodegradation, Swine waste, Phosphatase activity.
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