GROWTH DYNAMICS OF Pseudomonas putida (NBAII-RPF9) UNDER ABIOTIC STRESS CONDITIONS

Abstract

Experiments were conducted to know the growth variations adopted by Pseudomonas putida (NBAII-RPF 9) an abiotic stress tolerant plant growth promoter and biological control agent. Cell growth and survival was monitored under different time periods with two temperature regi mes (25 ̊ and 45o C) and data on viable cell count was analysed. P. putida (NBAII-RPF9) experienced a delayed lag phase when grown under heat stress as compared to its growth at normal temperature which was complemented by expression of higher whole cell protein concentration. Under heat stress increased protein content was noticed in lag phase. Whole cell protein analysis of P. putida (NBAII-RPF 9) by SDS-PAGE analysis showed expression of proteins ranging between 40KDa to 60KDa which match with molecular weight of major heat stress related proteins like GroEL and DnaK. This supported the fact that various proteins were triggered under st ress conditions to ensure its survival. KeywordsPseudomonas putida, heat stress, salt stress, growth curve, exponential phase, protein profile, HSPs, antagonism.