British journal of experimental pathology最新文献_第10页

Experimental dermatophytosis in mice: correlation between light and electron microscopic changes in primary, secondary and chronic infections. 小鼠实验性皮肤真菌病:原发性、继发性和慢性感染的光镜和电镜变化的相关性。

British journal of experimental pathology Pub Date : 1988-10-01

R J Hay, R A Calderon, C D Mackenzie

{"title":"Experimental dermatophytosis in mice: correlation between light and electron microscopic changes in primary, secondary and chronic infections.","authors":"R J Hay, R A Calderon, C D Mackenzie","doi":"","DOIUrl":"","url":null,"abstract":"The histopathological and electron microscopic features of experimental dermatophytosis due to Trichophyton quinckeanum in Balb/c mice have been studied in animals with primary, secondary and chronic infections. Infected animals all showed pathological changes with adherence of microconidia to keratinocytes within 4 h of infection. Other features were the early infiltration of neutrophils, the formation of a mycelial mass (scutulum) in the epidermis, and epidermal oedema. Increased thickness of the epidermis was measured within 3 days of infection, although this was mainly due to oedema. The main differences seen in secondary infections were the paucity of fungal elements, even after 24 h, a sustained increase in epidermal thickness, and the dense dermal infiltrate of mononuclear cells. Chronically infected animals showed similar changes to those at the peak of a primary infection, but in addition there were large numbers of mast cells in the dermis. Cells carrying Ia markers were identified in the epidermis (Langerhans cells) and the dermis (macrophages) in all infections and their distribution did not appear to change. Although recovery from infection has been correlated previously with T lymphocyte mediated responses an increase in the number of cell layers of the epidermis and a dense infiltrate of neutrophils at the zone of infection were both seen within 2 days of infection. It is suggested that neutrophil killing of fungi and increased epidermal proliferation, not dependent on T cell activation, may also be implicated in host defence against dermatophytes.","PeriodicalId":9248,"journal":{"name":"British journal of experimental pathology","volume":"69 5","pages":"703-16"},"PeriodicalIF":0.0,"publicationDate":"1988-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2013278/pdf/brjexppathol00005-0100.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14326865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reversible myocardial damage in gerbil brain ischaemia and its prevention by beta-adrenergic blockade. 沙鼠脑缺血的可逆性心肌损伤及其β -肾上腺素能阻断的预防。

British journal of experimental pathology Pub Date : 1988-10-01

A Kolin, A Brezina, J A Kellen, A J Lewis, J W Norris

{"title":"Reversible myocardial damage in gerbil brain ischaemia and its prevention by beta-adrenergic blockade.","authors":"A Kolin, A Brezina, J A Kellen, A J Lewis, J W Norris","doi":"","DOIUrl":"","url":null,"abstract":"Acute cerebral infarction in gerbils, produced by unilateral carotid ligation, was used as a model to investigate secondary myocardial changes. The extent of the myocardial damage revealed by succinic dehydrogenase (SDH) histochemistry and by release of myocardial creatine phosphokinase (MB-CK) was measured in gerbils sacrificed from 3 to 48 h after either carotid ligation, carotid isolation only or skin incision only. For technical reasons dead animals were excluded from analysis. Of surviving ligated animals 74% developed neurological deficits related to brain ischaemia. A significant weight increase in the ipsilateral hemisphere was found at 6-10 h, and maximal histological damage at 16 h, both partially reversible thereafter. Non-ligated animals did not develop neurological changes, and showed neither brain swelling nor cerebral histopathology. Extensive cardiac damage was shown by the SDH method from 3 h postoperatively, and confirmed by the elevated serum levels of MB-CK in the carotid-ligated group. The SDH changes were identical with those described in the hearts of patients with acute intracranial lesions, and appeared to be reversible. The effect of beta-adrenergic blockade was assessed in this model. Metoprolol tartrate injected intraperitoneally 3 h before and 1 h after carotid ligation (10 mg/kg each dose) significantly decreased the extent of myocardial damage as estimated both with SDH histochemistry and MB-CK serum levels. It had no effect on the ischaemic brain changes. These results strongly support the concept of catecholamine mediation of myocardial injury resulting from acute brain lesions.","PeriodicalId":9248,"journal":{"name":"British journal of experimental pathology","volume":"69 5","pages":"621-30"},"PeriodicalIF":0.0,"publicationDate":"1988-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2013282/pdf/brjexppathol00005-0020.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14326987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial myopathy in rats fed with a diet containing beta-guanidine propionic acid, an inhibitor of creatine entry in muscle cells. 喂食含有β -胍丙酸(一种肌酸进入肌肉细胞的抑制剂)的饮食的大鼠线粒体肌病。

British journal of experimental pathology Pub Date : 1988-10-01

Z Gori, V De Tata, M Pollera, E Bergamini

引用次数: 0

The effect of a toxin from Bacteroides ureolyticus on the mucosal epithelium of human and bovine oviducts. 解尿杆菌毒素对人和牛输卵管粘膜上皮的影响。

British journal of experimental pathology Pub Date : 1988-10-01

E A Fontaine, J B Clark, D Abeck, D Taylor-Robinson

引用次数: 0

Ultrastructural studies of a lysosomal enzyme during lymphocyte activation. 淋巴细胞活化过程中溶酶体酶的超微结构研究。

British journal of experimental pathology Pub Date : 1988-10-01

G Bou-Gharios, J Moss, D Abraham, T Partridge, I Olsen

引用次数: 0

The expression of MHC antigens on cultured oral keratinocytes and relationship to malignancy. 培养口腔角化细胞MHC抗原的表达及其与恶性肿瘤的关系。

British journal of experimental pathology Pub Date : 1988-10-01

I J Crane, S Q Rice, J Luker, L de Gay, C Scully, S S Prime

引用次数: 0

Increased nerve polyol levels in experimental diabetes and their reversal by Sorbinil. 实验性糖尿病患者神经多元醇水平升高及山梨醇的逆转作用。

British journal of experimental pathology Pub Date : 1988-10-01

P H Whiting, I S Ross

{"title":"Increased nerve polyol levels in experimental diabetes and their reversal by Sorbinil.","authors":"P H Whiting, I S Ross","doi":"","DOIUrl":"","url":null,"abstract":"Sciatic nerve glucose, sorbitol, fructose and myo-inositol levels were measured over a 24-week period in rats made diabetic using a single intraperitoneal injection of streptozotocin at 45 mg kg-1 body weight. In addition, the effect of the aldose reductase inhibitor Sorbinil (Pfizer Ltd) at 25 mg kg-1 day-1 in reversing the accumulation of nerve polyols following 8 weeks of diabetes was investigated. Following induction of diabetes 47, 471 and 456% increases and a 43% decrease in sciatic nerve glucose, sorbitol, fructose and myo-inositol concentrations (mumol g-1 wet weight) respectively, were observed by day 14. Over the remainder of the experimental course untreated diabetic control animals demonstrated relatively consistent elevations in sciatic nerve glucose, sorbitol and fructose. Although a significant, progressive reduction in sciatic nerve myo-inositol to 30% of onset values was observed over the first 84 days of the study, this was followed by a spontaneous partial recovery (31%) over the remainder of the experimental course. However, sciatic nerve myo-inositol levels at the end of the study were still significantly lower than onset values (P less than 0.01). Sorbinil treatment, initiated after 8 weeks of diabetes, and without effect on sciatic nerve glucose levels, normalized sorbitol concentrations following 4, 8, or 12 weeks of treatment but only partially reversed the accumulation of fructose by 368, 161 and 199%, compared to age-matched non-diabetic control values, at the above times, respectively. Mean myo-inositol levels were progressively increased following Sorbinil treatment over the experimental period, although the increase was only significant, compared to results from untreated diabetic animals, at weeks 4 and 16.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":9248,"journal":{"name":"British journal of experimental pathology","volume":"69 5","pages":"697-702"},"PeriodicalIF":0.0,"publicationDate":"1988-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2013281/pdf/brjexppathol00005-0094.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14274348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical nature and cellular origin of amyloid enhancing factor (AEF) as determined by anti-AEF antibody. 淀粉样蛋白增强因子(AEF)的生化性质及细胞来源

British journal of experimental pathology Pub Date : 1988-10-01

K Alizadeh-Khiavi, Z Ali-Khan

{"title":"Biochemical nature and cellular origin of amyloid enhancing factor (AEF) as determined by anti-AEF antibody.","authors":"K Alizadeh-Khiavi, Z Ali-Khan","doi":"","DOIUrl":"","url":null,"abstract":"Low ionic strength acidic buffer, Sephadex G-200 and Benzamidine-Sepharose (BZ) gel chromatography, have been used for the partial purification of alveolar hydatid cyst (AHC) induced amyloid enhancing factor (AEF). BZ-gel bound AEF (AEF-BZ) demonstrated AEF activity in the mouse bioassay, proteolytic activity against Hide powder azure showed two major and three minor peptides on SDS-PAGE. Pretreatment of AEF-BZ with 10 mM phenylmethylsulphonyl fluoride or 20 mM p-chloromercuribenzoic acid completely abolished its bioactivity in vivo and proteolytic activity in vitro. Polyclonal anti-AEF antibody (AAA) was generated which on passive transfer into mice completely abolished the bioactivity of both casein-induced, or AHC-induced AEF. The AAA absorbed on Sepharose gel conjugated to normal mouse serum developed one common precipitin band between AE and AEF-positive sera from AHC-infected and old retired mice and in immunostaining it bound to the cytoplasmic granular components of a majority of splenic and peritoneal leucocytes from AHC-infected mice. In contrast, only a few normal mouse leucocytes showed positive staining. We suggest that AEF, in all probability, is a serine/thiol protease of leucocyte origin whose intracellular and humoral concentrations increase significantly during amyloidosis. The role of lysosomal proteases and anti-AEF antibody which has been successfully generated for the first time is discussed with reference to the origin of AEF and its presumed biological function in amyloidogenesis.","PeriodicalId":9248,"journal":{"name":"British journal of experimental pathology","volume":"69 5","pages":"605-19"},"PeriodicalIF":0.0,"publicationDate":"1988-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2013270/pdf/brjexppathol00005-0005.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14190241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative histological analysis of antigen-induced arthritis in the rabbit. 兔抗原性关节炎的定量组织学分析。

British journal of experimental pathology Pub Date : 1988-10-01

J C Edwards, N Read, B Trefty, J Coulstock, B Henderson

引用次数: 0

Fibrinolysis by rat mesothelial cells in vitro: the effect of mineral dusts at non-toxic doses. 体外大鼠间皮细胞纤维蛋白溶解:无毒性剂量矿物粉尘的作用。

British journal of experimental pathology Pub Date : 1988-08-01

K Donaldson, G M Brown, R E Bolton, J M Davis

引用次数: 0