G Bou-Gharios, J Moss, D Abraham, T Partridge, I Olsen
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Ultrastructural studies of a lysosomal enzyme during lymphocyte activation.
A post-embedding immunogold technique has been used for the ultrastructural localization of a lysosomal enzyme, beta-glucuronidase, in resting and activated T- and B-lymphocytes. The results presented here show that mitogen-induced stimulation of T- and B-cells was associated with an increase in the amount of enzyme in the Golgi complex and rough endoplasmic reticulum, organelles which were rarely present in the resting lymphocytes.
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