British Journal of Biomedical Science最新文献

{"title":"Association of Vitamin D Gene Polymorphisms With HCV Infection Outcome.","authors":"M Neamatallah, M S Serria, M El-Bendary, A-H El-Gilany, A Alhawarey, S Abed, Y A Setate, O A Ammar","doi":"10.3389/bjbs.2021.10237","DOIUrl":"10.3389/bjbs.2021.10237","url":null,"abstract":"<p><p><b>Background:</b> Vitamin D derivatives and their receptor (VDR) are immune-response modulators in many diseases including malignancies, metabolic conditions, and infections. We hypothesized that one or more variants of <i>VDR</i> single nucleotide polymorphisms (SNPs) are associated with hepatocellular carcinoma (HCC) in hepatitis C virus (HCV) cirrhotic patients. <b>Materials and Methods:</b> A total of 861 subjects were recruited and classified as spontaneous viral clearance (SVC, <i>n</i> = 127), chronic hepatic cirrhosis (CHC, <i>n</i> = 392), and HCC (<i>n</i> = 342). Standard routine laboratory tests were performed and clinical features noted. All individuals were genotyped for seven SNPs spanning the <i>VDR</i> using real-time PCR. <b>Results:</b> Genotype frequencies of SNPs rs7970376, rs11568820, rs4516035, rs2228570 (Fok1), rs1544410 (Bsm-1), and rs731236 (Taq1), but not rs739837, were variously altered in CHC and HCC compared with SVC, and in HCC compared to CHC (all <i>p</i> < 0.001). The most powerful was rs7970376, which brought an OR (95% CI) of 7.14 (4.64-10.98) for HCC compared to SVC (<i>p</i> = 0.001). The carriage of the AGTAC haplotype of five SNPs were linked to CHC compared to SVC at OR 2.88 [95% CI 1.2-6.9] (<i>p</i> = 0.017) and with HCC compared to CHC at OR 1.54 [95% CI = 1.04-2.27 (<i>p</i> = 0.031). <b>Conclusion:</b> SNPs in <i>VDR</i> may have a potential role in the outcomes of patients with HCV infection. <i>VDR</i> SNPs; rs7970376, rs11568820, rs4516035, rs2228570 (Fok1), rs1544410 (Bsm-1), and rs731236 (Taq1) could be used as molecular markers to predict the risk of HCC.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2022-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983825/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40631252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Case Report: Recessive Dystrophic Epidermolysis Bullosa With Severe Esophageal Stenosis: A Case Report and Literature Review.","authors":"Zhen Xu,&nbsp;Tianqiao Huang,&nbsp;Min Pan,&nbsp;Yichuan Huang,&nbsp;Yan Jiang","doi":"10.3389/bjbs.2022.10200","DOIUrl":"https://doi.org/10.3389/bjbs.2022.10200","url":null,"abstract":"Epidermolysis bullosa (EB) is a rare genetic disease that has no effective management or cure. Patients with EBmaymanifest with skin andmucous membrane fragility, blisters, erosions and scars. Based on the 2014 diagnosis and treatment guidelines, EB can be divided into four types: EB simplex (EBS), junctional EB (JEB), dystrophic EB (DEB), and Kindler syndrome (1). DEB usually affects the skin and nails at birth, which can be divided into two subtypes, namely the dominant dystrophic epidermolysis bullosa (DDEB) and recessive dystrophic epidermolysis bullosa (RDEB) (2). Furthermore, based on different clinical features, RDEB has been classified into severe Hallopeau-Siemens type (RDEB-HS) or the milder form called RDEB-nHS. Patients with RDEBHS have systemic lesions and scars on hands and feet, leading to finger fusion and severe mucosal involvement, while those with RDEB-nHS may have local or systemic mild dermatological manifestations, mostly without finger fusion and without the involvement of the extradermal organs (3). Here, we report the case of a male patient with severe esophageal stricture due to recessive dystrophic EB. Currently, there is no effective treatment for EB complicated with severe esophageal stricture, although esophageal dilation and gastrostomy may be attempted.","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302543/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40412136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Angiogenesis, Metabolism, Endothelial and Platelet Markers in Diabetes and Cardiovascular Disease.","authors":"A D Blann,&nbsp;J E Brown,&nbsp;R Heitmar","doi":"10.3389/bjbs.2022.10313","DOIUrl":"https://doi.org/10.3389/bjbs.2022.10313","url":null,"abstract":"<p><p><b>Introduction:</b> Diabetes is a leading risk factor for cardiovascular disease (CVD), the pathophysiology of both being linked to metabolic, endothelial, renal, angiogenic and platelet abnormalities. We hypothesised that abnormalities in these systems are more adverse in those whose CVD is compounded by diabetes, compared to those with diabetes or CVD alone. <b>Materials and methods:</b> Serum or plasma from 66 patients with diabetes alone, 76 with CVD alone, and 70 with both diabetes and CVD i.e. diabetic cardiovascular disease, was probed for markers of angiogenesis [angiopoietin 1 and 2, vascular endothelial growth factor (VEGF) and endoglin], metabolic [soluble receptor for advanced glycation products (sRAGE), leptin, lipocalin-2, interleukin-8, and cystatin-C], the endothelium (von Willebrand factor, endothelial microparticles and soluble E selectin)], and the platelet (platelet microparticles and soluble P selectin) by ELISA, Luminex or flow cytometry. <b>Results:</b> VEGF (<i>p</i> = 0.04), von Willebrand factor (<i>p</i> = 0.001) and endothelial microparticles (<i>p</i> = 0.042) were all higher in diabetic cardiovascular disease than in diabetes alone and cardiovascular disease alone. Soluble E selectin was higher in diabetic cardiovascular disease than in diabetes alone (<i>p</i> = 0.045), whilst cystatin-C (<i>p</i> = 0.004) and soluble P selectin (<i>p</i> < 0.001) were higher in diabetes and diabetic cardiovascular disease than in cardiovascular disease alone. There were no differences in angiopoietin 1 or 2, endoglin, sRAGE, leptin, lipocalin-2, or interleukin-8. <b>Conclusion:</b> Angiopoietin 1 or 2, endoglin, sRAGE, leptin, lipocalin-2, interleukin-8, and cystatin-c cannot differentiate diabetes from cardiovascular disease, or both conditions combined. Our data point to a more adverse endothelial (von Willebrand factor, endothelial microparticles), and angiogenic profile (VEGF) in those with diabetic cardiovascular disease, supporting the view that this group should be targeted more aggressively.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40435894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding Role of DNA Repair and Cytochrome p-450 Gene Polymorphisms in Cervical Cancer Patient Treated With Concomitant Chemoradiation.","authors":"Mohammad Abbas,&nbsp;Vandana Singh Kushwaha,&nbsp;Kirti Srivastava,&nbsp;Monisha Banerjee","doi":"10.3389/bjbs.2021.10120","DOIUrl":"https://doi.org/10.3389/bjbs.2021.10120","url":null,"abstract":"<p><p><b>Background:</b> Evidences suggest that single nucleotide polymorphisms (SNPs) can be considered as potential biomarkers for disease progression and therapeutic response in cervical cancer. The present study investigated the association of <i>CYP1A1</i> T>C (rs4646903), <i>CYP1A1</i> A>G (rs1048943), <i>CYP2E1</i> T>A (rs6413432), <i>RAD51</i> G>C (rs1801320), <i>XRCC1</i> G>A (rs25487), <i>XRCC2</i> G>A (rs3218536) and <i>XRCC3</i> C>T (rs861539) polymorphisms with treatment outcome of cisplatin based chemoradiation (CRT). <b>Methods:</b> Total 227 cervical cancer cases, treated with the same chemoradiotherapy regimen were selected for the study. Genotyping analysis was performed by PCR-restriction fragment length polymorphisms (PCR-RFLP). Treatment response was evaluated by Response Evaluation Criteria in Solid Tumors (RECIST). Association of all clinical data (responses, recurrence and survival of patients) and single nucleotide polymorphisms (SNPs) was analysed by using SPSS (version 21.0). <b>Results:</b> Patients with TA/AA genotype of <i>CYP2E1</i> T>A polymorphism showed significantly poor response while those with GC/CC genotype of <i>RAD51</i> G>C showed better response (<i>p</i> = 0.008, <i>p</i> = 0.014 respectively). Death was significantly higher in patients with GG genotypes of <i>RAD51</i> G>C and <i>XRCC1</i> G>A (<i>p</i> = 0.006, <i>p</i> = 0.002 respectively). Women with GC+CC genotype of <i>RAD51</i> G>C and AG+GG of <i>XRCC1</i> showed better survival and also reduced risk of death (HR = 0.489, <i>p</i> = 0.008; HR = 0.484, <i>p</i> = 0.003 respectively). <b>Conclusion:</b> Results suggested that <i>CYP2E1</i> T>A (rs6413432), <i>RAD51</i> G>C (rs1801320), and <i>XRCC1</i> G>A (rs25487) polymorphisms may be used as predictive markers for clinical outcomes in cervical cancer patients undergoing cisplatin based concomitant chemoradiotherapy.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8915685/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40412139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Significant Association of Variable Number Tandem Repeat Polymorphism rs58335419 in the MIR137 Gene With the Risk of Gastric and Colon Cancers.","authors":"Pegah Jafari,&nbsp;Sedighe Baghernia,&nbsp;Mehdi Moghanibashi,&nbsp;Parisa Mohamadynejad","doi":"10.3389/bjbs.2021.10095","DOIUrl":"https://doi.org/10.3389/bjbs.2021.10095","url":null,"abstract":"<p><p><b>The purpose of the article:</b> The MIR137 gene acts as a tumor-suppressor gene in colon and gastric cancers. The aim of this study was to investigate the association of functional variable number tandem repeat (VNTR) polymorphism rs58335419 locating in the upstream of the MIR137 gene with the risk of colon and gastric cancers. <b>Materials and methods:</b> Totally, 429 individuals were contributed in the study, including 154 colon and 120 gastric cancer patients and 155 healthy controls. The target VNTR was genotyped using PCR and electrophoresis for all samples. Statistical analysis was performed using SPSS 21.0 software and by T, χ2 and logistic regression tests. <b>Results:</b> Excluding the rare genotypes, our results showed that genotype 3/5 (95% CI = 1.08-3.73, OR = 2.01, <i>p</i> = 0.026) significantly increased the risk of colon cancer but not gastric cancer (95% CI = 0.88-3.30, OR = 1.70, <i>p</i> = 0.114). Also, in the stratification analysis for VNTRs and sex, genotypes 3/4 (95% CI = 1.00-6.07, OR = 2.46, <i>p</i> = 0.049) and 3/5 (95% CI = 1.25-7.18, OR = 2.99, <i>p</i> = 0.014) significantly increased the risk of colon cancer in men but not in women. In addition, all genotypes including the rare genotypes as a group, significantly increase the risk of gastric (95% CI = 1.14-3.00, OR = 1.85, <i>p</i> = 0.012) and colon (95% CI = 1.38-3.43, OR = 2.17, <i>p</i> = 0.001) cancers compared to the genotype 3/3 as a reference. <b>Conclusion:</b> The results show that increasing the copy of VNTR in the MIR137 gene, increases the risk of colon and gastric cancers and can serve as a marker for susceptibility to colon and gastric cancers.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8915678/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40632707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Round Cells in Diagnostic Semen Analysis: A Guide for Laboratories and Clinicians.","authors":"S Long,&nbsp;S Kenworthy","doi":"10.3389/bjbs.2021.10129","DOIUrl":"https://doi.org/10.3389/bjbs.2021.10129","url":null,"abstract":"<p><p>Round cells in seminal fluid are defined as either leucocytes or immature germ cells. Laboratories undertaking semen analysis often report these combined as a concentration, with no further review, comment or direction for clinician action or review. Although numerous publications discuss the possible clinical relevance of these cells (particularly leucocytes) in infertility, the methods employed to differentiate them are often beyond the scope of most diagnostic laboratories. This paper aims to support healthcare scientists in understanding the clinical significance of round cells and aid their identification, differentiation and interpretation. This will support the quality of care the patient receives and direct clinicians to further considerations that may be appropriate for their patient and should consequently reduce indiscriminate and unnecessary use of antibiotics.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8915675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40632706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isocitrate Dehydrogenase <i>IDH1</i> and <i>IDH2</i> Mutations in Human Cancer: Prognostic Implications for Gliomas.","authors":"A K Murugan,&nbsp;A S Alzahrani","doi":"10.3389/bjbs.2021.10208","DOIUrl":"https://doi.org/10.3389/bjbs.2021.10208","url":null,"abstract":"<p><p><b>Background:</b> There are isolated reports of mutations in genes for isocitrate dehydrogenases (<i>IDH1</i> and <i>IDH2</i>), but few have been examined in a large number of different malignancies. We aimed to analyze mutational prevalence of these genes in a large series of cancers and determine their significance in most mutated phenotype. <b>Methods:</b> We analyzed the frequencies of <i>IDH1</i> and <i>IDH2</i> mutations in 14,726 malignancies of 37 cancers. Furthermore, we examined these mutations in the most frequent cancer (gliomas, 923 cases) from a single cohort, and determined their clinical significance. <b>Results:</b> <i>IDH1</i> mutations were present in 3% (473/14,726) of cancers. The highest frequencies were in oligodendrogliomas (91/102, 89%), anaplastic oligodendrogliomas (40/46, 87%), and diffuse astrocytomas (89/116, 77%). <i>IDH2</i> mutation was detected in <1% (83/14,726) of cancers, but were present in 13% (6/46) of anaplastic oligodendrogliomas, 9% (9/102) of oligodendrogliomas, and in 5% (2/39) of cutaneous squamous cell carcinomas. Further analyses of 923 gliomas revealed 34 and 1% of <i>IDH1</i> and <i>IDH2</i> mutations, respectively. In up to 342 months of follow-up, <i>IDH1</i> and <i>IDH2</i> mutations were significantly linked with better overall (OS) (both <i>p</i> = 0.01) and progression-free survival (PFS) (<i>p</i> = 0.01; <i>p</i> = 0.004), respectively. <b>Conclusion:</b> <i>IDH1</i> and <i>IDH2</i> are often mutated in a tissue-specific manner, most commonly in gliomas. Mutation in both genes is linked to OS and PFS. Our findings suggest that these genes are promising therapeutic targets and strong prognostic biomarkers in gliomas.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8915566/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40435895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotypes and Haplotypes in the <i>AXIN2</i> and <i>TCF7L2</i> Genes are Associated With Susceptibility and With Clinicopathological Characteristics in Breast Cancer Patients.","authors":"M A Rosales-Reynoso,&nbsp;V Rosas-Enríquez,&nbsp;A M Saucedo-Sariñana,&nbsp;M Pérez-Coria,&nbsp;M P Gallegos-Arreola,&nbsp;E Salas-González,&nbsp;P Barros-Núñez,&nbsp;C I Juárez-Vázquez,&nbsp;S E Flores-Martínez,&nbsp;J Sánchez-Corona","doi":"10.3389/bjbs.2021.10211","DOIUrl":"https://doi.org/10.3389/bjbs.2021.10211","url":null,"abstract":"<p><p><b>Background:</b> Breast cancer is a multifactorial disease whose genetic susceptibility is related to polymorphic variants of cell proliferation and migration pathways. Variants in <i>AXIN2</i> and <i>TCF7L2</i> in the Wnt-β catenin pathway have been associated with different types of cancer; however, little is known about its role in breast cancer. This study tests the hypothesis of links between <i>AXIN2</i> rs1133683 and rs2240308, and <i>TCF7L2</i> rs7903146 and rs12255372 variants in breast cancer. <b>Methods:</b> Peripheral blood samples were obtained from 404 women (202 patients and 202 control females). The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methodology was used to identify the gene variants. <b>Results:</b> The <i>AXIN2</i> rs2240308 (C > T), and <i>TCF7L2</i> rs7903146 (C > T) and rs12255372 (G > T) variants were associated with breast cancer and with age, TNM stage, and histologic-molecular subtype (<i>p</i> = 0.001). Likewise, the haplotype T-T in the <i>TCF7L2</i> gene (rs7903146-rs12253372) was significantly related with breast cancer (OR = 2.66, 95%, CI = 1.64-4.30, <i>p</i> = 0.001). <b>Conclusion:</b> Our data show a link between <i>AXIN2</i> rs2240308 and <i>TCF7L2</i> rs7903146 and rs12255372 variants in breast cancer, and speculate this may be important in pathogenesis.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8915722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40412135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mi-RNA-93 and Mi-RNA-152 in the Diagnosis of Type 2 Diabetes and Diabetic Retinopathy.","authors":"A A Saleh, S M El-Hefnawy, Z A Kasemy, A A Alhagaa, M Z Nooh, E S Arafat","doi":"10.3389/bjbs.2021.10192","DOIUrl":"10.3389/bjbs.2021.10192","url":null,"abstract":"<p><p><b>Background and Aim:</b> Diabetes mellitus (DM) is a chronic disorder with diabetic retinopathy (DR) as one of its main microvascular outcomes, being a prime cause of vision loss. Dysregulation of microRNAs (miRNAs) has been associated with some diabetic microvascular complications such as diabetic retinopathy. This hypothesised changes in the serum of miR-93 and miR-152 in diabetes and diabetic retinopathy. <b>Methods:</b> The study cohort consisted of 80 healthy volunteers, 80 type 2 diabetic patients, and 80 diabetic retinopathy patients, of whom 40 had proliferative (PDR) and 40 non-proliferative retinopathy (NPDR). Serum fasting and 2-hour postprandial glucose (2hPP), glycated haemoglobin (HbA1c), fasting insulin, and HOMA-IR were evaluated by routine methods, miR-93 and miR-152 expression by quantitative real-time PCR. <b>Results:</b> FBG, 2hPP, fasting insulin, HOMA-IR, and miR-152 showed an increasing trend across groups while miR-93 showed a decreasing trend (all <i>p</i> < 0.001). Binary logistic regression analysis for prediction of DR found that the most significant were miR-152 (OR 1.37, 95% CI: 1.18-1.58, <0.001), BMI (1.13, [1.07-1.31], <i>p</i> = 0.004), duration of disease (1.29 [1.04-1.6] <i>p</i> = 0.018), and miR-152 (0.01, [0.0-0.47] <i>p</i> = 0.019). The most significant predictors of PDR were miR-152 (OR = 1.47, 95% CI: 1.12-1.92, <i>p</i> = 0.005), HOMA-IR (2.66 [1.30-5.45] <i>p</i> = 0.007), and miR-93 (0.25 [0.07-0.86] <i>p</i> = 0.028). <b>Conclusion:</b> MiR-93 and miR-152 can differentiate patients with diabetes and those with DR. Both miRNAs might be potential biomarkers for diabetes and diabetic retinopathy, and specifically for proliferative diabetic retinopathy.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2022-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8915732/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40435898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Thyroid Tissue Status on the Cut-Off Value of Lymph Node Fine-Needle Aspiration Thyroglobulin Measurements in Papillary Thyroid Cancer.","authors":"L Zhai,&nbsp;W Jiang,&nbsp;Y Zang,&nbsp;Y Gao,&nbsp;D Jiang,&nbsp;Q Tian,&nbsp;C Zhao","doi":"10.3389/bjbs.2021.10210","DOIUrl":"https://doi.org/10.3389/bjbs.2021.10210","url":null,"abstract":"<p><p><b>Objective:</b> To study the optimal cut-off value of thyroglobulin measurement in a fine-needle aspiration (FNA-Tg) in diagnosing malignant lymph nodes and benign lymph nodes (LNs) according to the thyroid tissue status. <b>Methods:</b> A total of 517 LNs were aspirated: 401 preoperative LNs, 42 LNs after subtotal thyroidectomy and 74 suspected LNs after total thyroidectomy. The cut-off value of FNA-Tg was obtained from receiver operating characteristic (ROC) analysis. The cut-off value with the best diagnostic performance was then obtained by comparing different cut-off values from other studies. <b>Results:</b> LN FNA-Tg levels differed between preoperative and total thyroid disease (<i>p</i> < 0.001) and subtotal thyroidectomy and total thyroidectomy (<i>p</i> = 0.03), but not between preoperative and subtotal thyroidectomy (<i>p</i> = 1.00). Accordingly, those 443 LNs with preoperative and subtotal thyroidectomy were compared to those 74 without thyroid tissue. The optimal cut-off value in thyroid tissue group was 19.4 ng/ml and the area under the ROC curve (AUC) was 0.95 (95% CI 0.92-0.97). The optimal cut-off value in thyroid tissue absence group was 1.2 ng/ml and the AUC was 0.93 (0.85-0.98). After the analysis and comparison of multiple cut-off values, the optimal diagnostic performance was still found to be 19.4 ng/ml and 1.2 ng/ml. <b>Conclusion:</b> The influential factors of FNA-Tg are still controversial, and the optimal cut-off value of FNA-Tg can be determined based on the presence or absence of thyroid tissue. FNA-Tg can be used as an important auxiliary method for diagnosing cervical metastatic LNs of thyroid cancer.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8915611/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40631255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}