British Journal of Biomedical Science最新文献_第7页

CCAT 1- A Pivotal Oncogenic Long Non-Coding RNA in Colorectal Cancer. ccat1 -结直肠癌中关键的致癌长链非编码RNA。

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2023-01-01 DOI: 10.3389/bjbs.2023.11103

Xiew Leng Liau, Shamala Salvamani, Baskaran Gunasekaran, Dinesh Kumar Chellappan, Anthony Rhodes, Vaidehi Ulaganathan, Yee Lian Tiong

{"title":"CCAT 1- A Pivotal Oncogenic Long Non-Coding RNA in Colorectal Cancer.","authors":"Xiew Leng Liau, Shamala Salvamani, Baskaran Gunasekaran, Dinesh Kumar Chellappan, Anthony Rhodes, Vaidehi Ulaganathan, Yee Lian Tiong","doi":"10.3389/bjbs.2023.11103","DOIUrl":"https://doi.org/10.3389/bjbs.2023.11103","url":null,"abstract":"Colorectal cancer (CRC) is ranked as the third most common cancer and second deadliest cancer in both men and women in the world. Currently, the cure rate and 5-year survival rate of CRC patients remain relatively low. Therefore, discovering a novel molecular biomarker that can be used to improve CRC screening, diagnosis, prognosis, and treatment would be beneficial. Long non-coding RNA colon cancer-associated transcript 1 (CCAT 1) has been found overexpressed in CRC and is associated with CRC tumorigenesis and treatment outcome. CCAT 1 has a high degree of specificity and sensitivity, it is readily detected in CRC tissues and is significantly overexpressed in both premalignant and malignant CRC tissues. Besides, CCAT 1 is associated with clinical manifestation and advanced features of CRC, such as lymph node metastasis, high tumor node metastasis stage, differentiation, invasion, and distant metastasis. In addition, they can upregulate oncogenic c-MYC and negatively modulate microRNAs via different mechanisms of action. Furthermore, dysregulated CCAT 1 also enhances the chemoresistance in CRC cells while downregulation of them reverses the malignant phenotypes of cancer cells. In brief, CCAT 1 serves as a potential screening, diagnostic and prognostic biomarker in CRC, it also serves as a potential therapeutic marker to treat CRC patients.","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"80 ","pages":"11103"},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10070472/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9276695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Long Non-Coding RNA LINC01929 Facilitates Cell Proliferation and Metastasis as a Competing Endogenous RNA Against MicroRNA miR-1179 in Non-Small Cell Lung Carcinoma. 长链非编码RNA LINC01929作为与MicroRNA miR-1179竞争的内源性RNA促进非小细胞肺癌细胞增殖和转移。

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2022-07-14 eCollection Date: 2022-01-01 DOI: 10.3389/bjbs.2022.10598

Tinghong Pan, Hui Wang, Shuai Wang, Feng Liu

{"title":"Long Non-Coding RNA LINC01929 Facilitates Cell Proliferation and Metastasis as a Competing Endogenous RNA Against MicroRNA miR-1179 in Non-Small Cell Lung Carcinoma.","authors":"Tinghong Pan, Hui Wang, Shuai Wang, Feng Liu","doi":"10.3389/bjbs.2022.10598","DOIUrl":"https://doi.org/10.3389/bjbs.2022.10598","url":null,"abstract":"Introduction: Non-small cell lung carcinoma (NSCLC) constitutes most lung cancers and has a poor prognosis. LncRNAs are a potential repository for the discovery of cancer prognostic markers. This study explored the role of LINC01929 in NSCLC, both the clinical prognostic significance and the mechanism of its influence on cells. Materials and Methods: LINC01929 levels in 143 pairs of NSCLC tissues and non-cancerous tissues were detected by RT-qPCR. Kaplan-Meier curves and multivariate Cox regression assays were generated for evaluating the prognostic values of LINC01929. To evaluate the cellular function, an XTT assay and transwell invasion assays were performed. Results: LINC01929 was up-regulated in NSCLC tissues compared with healthy tissues. A positive correlation was observed between LINC01929 expression level and tumor T (p = 0.002) or N stage (p = 0.010). Patients with higher LINC01929 levels had shorter overall survival (p = 0.009). Compared with other factors, high LINC01929 expression was significantly associated with poor survival in univariate Cox analysis (HR: 2.485, 95%CI: 1.220-5.060, p = 0.012). After multivariate Cox regression assays, LINC01929 was a independent prognostic factor (HR: 3.021, 95%CI: 1.377-6.628, p = 0.006). miR-1179 was a target miRNA of LINC01929. Inhibited expression of LINC01929 significantly reduced the proliferation, migration, and invasion of NSCLC cells by targeting miR-1179. Discussion: This study revealed the upregulation of LINC01929 in NSCLC. This study supports previous studies showing LINC01929 as a potential prognostic factor for NSCLC.","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":" ","pages":"10598"},"PeriodicalIF":1.9,"publicationDate":"2022-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40412133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Diagnostic and Prognostic Value Analysis of miR-206 in Asymptomatic Carotid Artery Stenosis. miR-206在无症状颈动脉狭窄中的诊断及预后价值分析。

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2022-07-06 eCollection Date: 2022-01-01 DOI: 10.3389/bjbs.2022.10592

Dancen Li, Jingjun Pan

{"title":"Diagnostic and Prognostic Value Analysis of miR-206 in Asymptomatic Carotid Artery Stenosis.","authors":"Dancen Li, Jingjun Pan","doi":"10.3389/bjbs.2022.10592","DOIUrl":"https://doi.org/10.3389/bjbs.2022.10592","url":null,"abstract":"Introduction: To investigate the expression level of miR-206 in serum of patients with asymptomatic carotid artery stenosis (CAS) and estimate the value of miR-206 in the diagnosis and prognosis of asymptomatic CAS. Methods: A total of 206 individuals enrolled in this study, including 105 CAS patients and 101 controls. RT-qPCR technology was applied to measure the relative level of miR-206, and Pearson's correlation coefficient was performed to analyze the relationship between carotid artery stenosis degree and miR-206 level. An ROC curve was drawn to assess the diagnostic value of miR-206 in asymptomatic CAS. The 5-year prognosis of asymptomatic CAS patients was tested using multivariate Cox regression analysis and Kaplan-Meier survival curve. Results: MiR-206 expression was reduced in asymptomatic CAS patients. The AUC of the ROC curve of miR-206 was 0.939, with a sensitivity of 86.70% and a specificity of 86.14%. The amount of CAS gradually increased with the decrease of miR-206 level. Seven-teen patients in the low miR-206 expression group developed CIEs, and 3 patients in the high miR-206 expression group developed CIEs during the 5-year follow-up. miR-206 and the amount of CAS were independent factors for the occurrence of CIEs within 5 years in asymptomatic CAS patients. Conclusion: Serum miR-206 has high diagnostic accuracy for asymptomatic CAS and has predictive value for the incidence of CIEs in patients within 5 years.","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":" ","pages":"10592"},"PeriodicalIF":1.9,"publicationDate":"2022-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302550/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40631251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Beneficial Modulatory Effects of Treatment With Bone Marrow Lysate on Hematopoietic Stem Cells and Myeloid Cells in Tumor-Bearing Mice. 骨髓裂解液对荷瘤小鼠造血干细胞和骨髓细胞的有益调节作用。

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2022-06-29 eCollection Date: 2022-01-01 DOI: 10.3389/bjbs.2022.10328

Mohamed L Salem, Kadry A El-Bakry, Eman H Moubark, Ashraf Sobh, Sohaila M Khalil

{"title":"Beneficial Modulatory Effects of Treatment With Bone Marrow Lysate on Hematopoietic Stem Cells and Myeloid Cells in Tumor-Bearing Mice.","authors":"Mohamed L Salem, Kadry A El-Bakry, Eman H Moubark, Ashraf Sobh, Sohaila M Khalil","doi":"10.3389/bjbs.2022.10328","DOIUrl":"https://doi.org/10.3389/bjbs.2022.10328","url":null,"abstract":"Introduction: Leukopenia is one of the major side effects of myelosuppressive chemotherapy such as cyclophosphamide (CTX). We and others have used CTX either alone or in combination with G-CSF for the mobilization of hematopoietic stem cells (HSCs). This mobilization can induce expansion of myeloid cells with immunosuppressive phenotype. In this pilot study, we aimed to test whether bone marrow lysate (BML)/CTX, a rich source of growth factors, can lower the expansion of myeloid cells with immunosuppressive phenotypes in tumor-bearing mice without interfering with the anti-tumor effects of CTX or with the mobilization of HSCs. Methods: Female CD1 mice were treated on day 0 with an i.p. injection of Ehrlich ascites carcinoma (EAC). On day 7, the mice were i.p. injected with CTX followed by s.c. injection of G-CSF for 5 consecutive days, single s.c. injection of BML/PBS or BML/CTX or single i.v. injection of BMC/PBS or BMC/CTX. Results: Treatment of EAC-bearing mice with BML/PBS or BML/CTX did not interfere with the anti-tumor effect of CTX. EAC increased the numbers of immature polymorphonuclear cells (iPMN; neutrophils) in both blood and spleen. Treatment of EAC-bearing mice with CTX further increased the numbers of these cells, which were decreased upon treatment with BML/CTX. Treatment with BML/PBS or BML/CTX increased the numbers of stem cells (C.Kit+Sca-1+) in BM; the effect of BML/CTX was higher, but with no significant effect on the numbers of HSCs. Future studies are needed to analyze the molecular components in BM lysate and to determine the underlying mechanisms.","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":" ","pages":"10328"},"PeriodicalIF":1.9,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302549/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40412138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of LncRNA CARD8-AS1 as a Potential Prognostic Biomarker Associated With Progression of Lung Adenocarcinoma. LncRNA CARD8-AS1作为肺腺癌进展相关的潜在预后生物标志物的鉴定

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2022-06-23 eCollection Date: 2022-01-01 DOI: 10.3389/bjbs.2022.10498

Yong Ji, Guoqing Zhang, Xingyi Zhang

{"title":"Identification of LncRNA CARD8-AS1 as a Potential Prognostic Biomarker Associated With Progression of Lung Adenocarcinoma.","authors":"Yong Ji, Guoqing Zhang, Xingyi Zhang","doi":"10.3389/bjbs.2022.10498","DOIUrl":"https://doi.org/10.3389/bjbs.2022.10498","url":null,"abstract":"Introduction: Long non-coding RNAs (lncRNAs) exhibit crucial roles in human tumors. However, the role of lncRNA CARD8-AS1 in lung adenocarcinoma remains elusive. This study investigated the role of CARD8-AS1 in lung adenocarcinoma. Materials and Methods: The expression of CARD8-AS1 was detected by RT-qPCR analysis and confirmed using an online database. The clinical value of CARD8-AS1 was evaluated using the Kaplan-Meier curve and multivariate Cox regression analyses. The effects of CARD8-AS1 on cancer cell proliferation, migration, and invasion potential were assessed through several cellular experiments. Western blot assay was used to measure Bcl-2 and Bax protein levels. The interaction among CARD8-AS1, miR-650, and Bax, was assessed using a dual-luciferase reporter assay. Results: The expression of CARD8-AS1 was decreased in lung adenocarcinoma tissues and cell lines (p < 0.001). Low expression of CARD8-AS1 was related to tumor size (p = 0.042), TNM stage (p = 0.021), lymph node metastasis (p = 0.025), and poor overall survival (p < 0.05). Elevated expression of CARD8-AS1 could suppress cellular viability, migration potential, and invasion ability (p < 0.05). The Bcl-2 protein levels were decreased while Bax levels were increased by overexpression of CARD8-AS1 (p < 0.001). miR-650 may thus be a direct target of CARD8-AS1 and Bax may be a direct target of miR-650. Discussion: CARD8-AS1 expression was downregulated in lung adenocarcinoma and associated with several clinical parameters. CARD8-AS1 exerted tumor-suppressive effects by targeting the miR-650 and then regulating Bax expression. CARD8-AS1/miR-650 may serve as novel prognostic biomarkers and potential therapeutic targets for the treatment of lung adenocarcinoma.","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":" ","pages":"10498"},"PeriodicalIF":1.9,"publicationDate":"2022-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302548/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40412137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Biomedical Science and New Frontiers. 生物医学科学与新领域。

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2022-06-20 eCollection Date: 2022-01-01 DOI: 10.3389/bjbs.2022.10638

Anthony Rhodes

引用次数: 1

The Microbiology of Non-aeruginosa Pseudomonas Isolated From Adults With Cystic Fibrosis: Criteria to Help Determine the Clinical Significance of Non-aeruginosa Pseudomonas in CF Lung Pathology. 囊性纤维化成人非铜绿假单胞菌的微生物学:帮助确定非铜绿假单胞菌在CF肺病理中的临床意义的标准

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2022-06-08 eCollection Date: 2022-01-01 DOI: 10.3389/bjbs.2022.10468

John E Moore, John McCaughan, Jacqueline C Rendall, Beverley C Millar

{"title":"The Microbiology of Non-aeruginosa Pseudomonas Isolated From Adults With Cystic Fibrosis: Criteria to Help Determine the Clinical Significance of Non-aeruginosa Pseudomonas in CF Lung Pathology.","authors":"John E Moore, John McCaughan, Jacqueline C Rendall, Beverley C Millar","doi":"10.3389/bjbs.2022.10468","DOIUrl":"https://doi.org/10.3389/bjbs.2022.10468","url":null,"abstract":"Introduction: There is a paucity of reports on non-aeruginosa Pseudomonas (NAPs) in cystic fibrosis, hence this study wished 1). to examine the diversity/frequency of NAPs in an adult CF population, 2) to compare/contrast the microbiology and genomics of NAPs to P. aeruginosa and 3) to propose clinical and laboratory criteria to help determine their clinical significance in CF lung pathology. Materials and Methods: Microbiological data was examined from 100 adult patients with cystic fibrosis from birth to present (31/12/2021), equating to 2455 patient years. 16S rDNA phylogenetic relatedness of NAPs was determined, as well as bioinformatical comparison of whole genomes of P. aeruginosa against P. fluorescens. Results: Ten species were isolated from this patient cohort during this time period, with three species, i.e., P. fluorescens, P. putida and P. stutzeri, accounting for the majority (87.5%) of non-aeruginosa reports. This is the first report of the isolation of P. fragi, P. nitroreducens, P. oryzihabitans and P. veronii in patients with cystic fibrosis. The mean time to first detection of any non-aeruginosa species was 183 months (15.25 years) [median = 229 months (19.1 years)], with a range from 11 months to 338 months (28.2 years). Several of the NAPs were closely related to P. aeruginosa. Discussion: NAPs were isolated infrequently and were transient colonisers of the CF airways, in those patients with CF in which they were isolated. A set of ten clinical and laboratory criteria are proposed to provide key indicators, as to the clinical importance of the non-aeruginosa species isolated.","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":" ","pages":"10468"},"PeriodicalIF":1.9,"publicationDate":"2022-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302546/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40600581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Comparison Between Dichloroacetate and Phenylbutyrate Treatment for Pyruvate Dehydrogenase Deficiency. 二氯乙酸酯与苯丁酸酯治疗丙酮酸脱氢酶缺乏症的比较。

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2022-05-19 eCollection Date: 2022-01-01 DOI: 10.3389/bjbs.2022.10382

Patricia Karissa, Timothy Simpson, Simon P Dawson, Teck Yew Low, Sook Hui Tay, Fatimah Diana Amin Nordin, Shamsul Mohd Zain, Pey Yee Lee, Yuh-Fen Pung

{"title":"Comparison Between Dichloroacetate and Phenylbutyrate Treatment for Pyruvate Dehydrogenase Deficiency.","authors":"Patricia Karissa, Timothy Simpson, Simon P Dawson, Teck Yew Low, Sook Hui Tay, Fatimah Diana Amin Nordin, Shamsul Mohd Zain, Pey Yee Lee, Yuh-Fen Pung","doi":"10.3389/bjbs.2022.10382","DOIUrl":"https://doi.org/10.3389/bjbs.2022.10382","url":null,"abstract":"Pyruvate dehydrogenase (PDH) deficiency is caused by a number of pathogenic variants and the most common are found in the PDHA1 gene. The PDHA1 gene encodes one of the subunits of the PDH enzyme found in a carbohydrate metabolism pathway involved in energy production. Pathogenic variants of PDHA1 gene usually impact the α-subunit of PDH causing energy reduction. It potentially leads to increased mortality in sufferers. Potential treatments for this disease include dichloroacetate and phenylbutyrate, previously used for other diseases such as cancer and maple syrup urine disease. However, not much is known about their efficacy in treating PDH deficiency. Effective treatment for PDH deficiency is crucial as carbohydrate is needed in a healthy diet and rice is the staple food for a large portion of the Asian population. This review analysed the efficacy of dichloroacetate and phenylbutyrate as potential treatments for PDH deficiency caused by PDHA1 pathogenic variants. Based on the findings of this review, dichloroacetate will have an effect on most PDHA1 pathogenic variant and can act as a temporary treatment to reduce the lactic acidosis, a common symptom of PDH deficiency. Phenylbutyrate can only be used on patients with certain pathogenic variants (p.P221L, p.R234G, p.G249R, p.R349C, p.R349H) on the PDH protein. It is hoped that the review would provide an insight into these treatments and improve the quality of lives for patients with PDH deficiency.","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":" ","pages":"10382"},"PeriodicalIF":1.9,"publicationDate":"2022-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40412134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Significant Association of DNASE1 Variable Number Tandem Repeats and Single Nucleotide Polymorphisms With Gastric Cancer. DNASE1可变数目串联重复序列和单核苷酸多态性与胃癌的显著关联。

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2022-05-13 eCollection Date: 2022-01-01 DOI: 10.3389/bjbs.2022.10526

Ali Kafil, Parisa Mohamadynejad, Mehdi Moghanibashi

{"title":"Significant Association of DNASE1 Variable Number Tandem Repeats and Single Nucleotide Polymorphisms With Gastric Cancer.","authors":"Ali Kafil, Parisa Mohamadynejad, Mehdi Moghanibashi","doi":"10.3389/bjbs.2022.10526","DOIUrl":"https://doi.org/10.3389/bjbs.2022.10526","url":null,"abstract":"Introduction: Defects in the apoptotic process are among the most important events involved in carcinogenesis, and defects in DNASE1, as one of the apoptotic machinery components, plays a role in various types of cancer. Previous studies have indicated significant differences in the DNASE1 polymorphisms in different populations. We hypothesized an association of two polymorphic sites in the exon 8 and the intron 4 of the DNASE1 gene with the risk of gastric cancer. Materials and Methods: The study was carried out on 120 gastric cancer patients and 120 age and sex adjusted controls using PCR and RFLP-PCR. Results: The genotype GG (rs1053874) in exon 8 of DNASE1 (odds ratio [95% confidence interval]) 4.65 [2.10-10.29], p < 0.001) and genotype 2/3 of variable number tandem repeat (VNTR) in the intron 4 (3.75 [1.56-9.01], p = 0.003) are both linked to gastric cancer. Conclusion: We propose that both polymorphic sites have a part to play in gastric cancer, and so may be useful diagnosis markers.","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":" ","pages":"10526"},"PeriodicalIF":1.9,"publicationDate":"2022-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302541/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40631256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The E23K Polymorphism of KCNJ11 and Diabetic Retinopathy in Northern Iran. 伊朗北部地区KCNJ11基因E23K多态性与糖尿病视网膜病变的关系

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2022-04-22 eCollection Date: 2022-01-01 DOI: 10.3389/bjbs.2021.10245

L Alidoust, F Ajamian, S Abbaspour, A Sharafshah, P Keshavarz

{"title":"The E23K Polymorphism of KCNJ11 and Diabetic Retinopathy in Northern Iran.","authors":"L Alidoust, F Ajamian, S Abbaspour, A Sharafshah, P Keshavarz","doi":"10.3389/bjbs.2021.10245","DOIUrl":"https://doi.org/10.3389/bjbs.2021.10245","url":null,"abstract":"Background: Diabetic Retinopathy (DR) is one of the most severe micro-vascular complications of diabetes mellitus (DM), involving interactions between environmental and genetic risk factors. KCNJ11 gene has a key role in insulin secretion and is of substantial interest in various populations. Methods: A population-based association of 524 T2DM patients was performed to delineate the genetic influence of KCNJ11 polymorphisms (rs5219, c.67A>G or E23K) on the risk of DR in an Iranian population. Genotyping was performed using TaqMan assay. Univariate and MLR analysis controlling for confounders was conducted to evaluate the association between rs5219 and DR. Results: No significant difference was observed in either genotypes distribution (p = 0.83) or allele frequency (p = 0.66) between T2DM individuals with and without DR in any models of inheritance. Genotype-phenotype association showed that DR group carrying GA genotypes, a significantly higher mean age was observed compared with two other genotypes (p = 0.04). MLR analysis indicated that HbAlc with adjusted OR of 1.84 (95% CI, 1.46-2.33, p = 0.00) and first-degree relatives of family history with adjusted OR of 2.85 (95% CI, 1.45-5.58, p = 0.002) were significantly associated with DR, but the c.67A>G genotype is not an independent predictor of retinopathy. Conclusion: Collectively, rs5219 was not associated with DR among Iranians with T2DM.","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":" ","pages":"10245"},"PeriodicalIF":1.9,"publicationDate":"2022-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073720/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40631250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0