LncRNA CARD8-AS1作为肺腺癌进展相关的潜在预后生物标志物的鉴定

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
ACS Applied Bio Materials Pub Date : 2022-06-23 eCollection Date: 2022-01-01 DOI:10.3389/bjbs.2022.10498
Yong Ji, Guoqing Zhang, Xingyi Zhang
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引用次数: 1

摘要

长链非编码rna (lncRNAs)在人类肿瘤中发挥着至关重要的作用。然而,lncRNA CARD8-AS1在肺腺癌中的作用尚不清楚。本研究探讨了CARD8-AS1在肺腺癌中的作用。材料与方法:采用RT-qPCR检测CARD8-AS1的表达,并通过在线数据库进行确认。采用Kaplan-Meier曲线和多变量Cox回归分析评价CARD8-AS1的临床价值。通过几个细胞实验评估了CARD8-AS1对癌细胞增殖、迁移和侵袭潜力的影响。Western blot法检测Bcl-2和Bax蛋白水平。使用双荧光素酶报告试验评估CARD8-AS1、miR-650和Bax之间的相互作用。结果:CARD8-AS1在肺腺癌组织和细胞系中的表达降低(p < 0.001)。CARD8-AS1低表达与肿瘤大小(p = 0.042)、TNM分期(p = 0.021)、淋巴结转移(p = 0.025)、总生存率差(p < 0.05)有关。升高CARD8-AS1表达可抑制细胞活力、迁移潜能和侵袭能力(p < 0.05)。过表达CARD8-AS1使Bcl-2蛋白水平降低,Bax蛋白水平升高(p < 0.001)。因此miR-650可能是CARD8-AS1的直接靶点,Bax可能是miR-650的直接靶点。讨论:CARD8-AS1在肺腺癌中表达下调,并与多个临床参数相关。CARD8-AS1通过靶向miR-650调控Bax表达发挥抑瘤作用。CARD8-AS1/miR-650可能作为肺腺癌治疗的新型预后生物标志物和潜在治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Identification of LncRNA CARD8-AS1 as a Potential Prognostic Biomarker Associated With Progression of Lung Adenocarcinoma.

Identification of LncRNA CARD8-AS1 as a Potential Prognostic Biomarker Associated With Progression of Lung Adenocarcinoma.

Identification of LncRNA CARD8-AS1 as a Potential Prognostic Biomarker Associated With Progression of Lung Adenocarcinoma.

Identification of LncRNA CARD8-AS1 as a Potential Prognostic Biomarker Associated With Progression of Lung Adenocarcinoma.

Introduction: Long non-coding RNAs (lncRNAs) exhibit crucial roles in human tumors. However, the role of lncRNA CARD8-AS1 in lung adenocarcinoma remains elusive. This study investigated the role of CARD8-AS1 in lung adenocarcinoma. Materials and Methods: The expression of CARD8-AS1 was detected by RT-qPCR analysis and confirmed using an online database. The clinical value of CARD8-AS1 was evaluated using the Kaplan-Meier curve and multivariate Cox regression analyses. The effects of CARD8-AS1 on cancer cell proliferation, migration, and invasion potential were assessed through several cellular experiments. Western blot assay was used to measure Bcl-2 and Bax protein levels. The interaction among CARD8-AS1, miR-650, and Bax, was assessed using a dual-luciferase reporter assay. Results: The expression of CARD8-AS1 was decreased in lung adenocarcinoma tissues and cell lines (p < 0.001). Low expression of CARD8-AS1 was related to tumor size (p = 0.042), TNM stage (p = 0.021), lymph node metastasis (p = 0.025), and poor overall survival (p < 0.05). Elevated expression of CARD8-AS1 could suppress cellular viability, migration potential, and invasion ability (p < 0.05). The Bcl-2 protein levels were decreased while Bax levels were increased by overexpression of CARD8-AS1 (p < 0.001). miR-650 may thus be a direct target of CARD8-AS1 and Bax may be a direct target of miR-650. Discussion: CARD8-AS1 expression was downregulated in lung adenocarcinoma and associated with several clinical parameters. CARD8-AS1 exerted tumor-suppressive effects by targeting the miR-650 and then regulating Bax expression. CARD8-AS1/miR-650 may serve as novel prognostic biomarkers and potential therapeutic targets for the treatment of lung adenocarcinoma.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
期刊介绍: ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.
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