British Journal of Biomedical Science最新文献_第10页

Overexpression of long non-coding RNA MCM3AP-AS1 in breast cancer tissues compared to adjacent non-tumour tissues. 与邻近非肿瘤组织相比，长链非编码RNA MCM3AP-AS1在乳腺癌组织中的过表达

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2021-04-01 Epub Date: 2020-09-07 DOI: 10.1080/09674845.2020.1798058

A Riahi, M Hosseinpour-Feizi, A Rajabi, M Akbarzadeh, V Montazeri, R Safaralizadeh

{"title":"Overexpression of long non-coding RNA MCM3AP-AS1 in breast cancer tissues compared to adjacent non-tumour tissues.","authors":"A Riahi, M Hosseinpour-Feizi, A Rajabi, M Akbarzadeh, V Montazeri, R Safaralizadeh","doi":"10.1080/09674845.2020.1798058","DOIUrl":"https://doi.org/10.1080/09674845.2020.1798058","url":null,"abstract":"Background: Altered expression of several long non-coding RNAs (lncRNAs) has been described in numerous malignancies, including breast cancer, and some may have a role in carcinogenesis. We hypothesised differences in the expression of lncRNA MCM3AP-AS1 in breast cancer tissues compared to nearby healthy tissues and potential links with clinical features.Methods: We tested our hypothesis in 102 pairs of breast cancer tumours and adjacent non-tumour tissues from female patients. After RNA extraction, cDNA synthesis was performed for all specimens. The differential gene expression was assessed using Quantitative Real-Time PCR Technique.Results: There was a significant overexpression of the lncRNAs in tumour tissues as compared with their adjacent non-tumour tissues (P < 0.001). Expression was significantly linked with the tumour oestrogen receptor expression (P = 0.023) and tumour progesterone receptor expression (P < 0.001). ROC analysis showed an AUC of 0.67 (95% CI 0.60-0.75) (P < 0.001) with sensitivity and specificity of 58% and 76%, respectively.Conclusion: The lncRNA MCM3AP-AS1 may be a novel breast cancer lncRNA with high expression levels in breast cancer patients' tissue. Further investigations are needed to confirm its uses as a potential molecular marker and therapeutic target.","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 2","pages":"53-57"},"PeriodicalIF":1.9,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1798058","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38163992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Value of serum collagen triple helix repeat containing-1(CTHRC1) and 14-3-3η protein compared to anti-CCP antibodies and anti-MCV antibodies in the diagnosis of rheumatoid arthritis. 血清胶原三螺旋重复包含-1(CTHRC1)和14-3-3η蛋白与抗ccp抗体和抗mcv抗体在类风湿关节炎诊断中的价值

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2021-04-01 Epub Date: 2020-09-07 DOI: 10.1080/09674845.2020.1810400

T Hu, Y Liu, L Tan, J Huang, J Yu, Y Wu, Z Pei, X Zhang, J Li, L Song, W Dai, Y Xiang

{"title":"Value of serum collagen triple helix repeat containing-1(CTHRC1) and 14-3-3η protein compared to anti-CCP antibodies and anti-MCV antibodies in the diagnosis of rheumatoid arthritis.","authors":"T Hu, Y Liu, L Tan, J Huang, J Yu, Y Wu, Z Pei, X Zhang, J Li, L Song, W Dai, Y Xiang","doi":"10.1080/09674845.2020.1810400","DOIUrl":"https://doi.org/10.1080/09674845.2020.1810400","url":null,"abstract":"Introduction: Serological markers are important in the diagnosis of rheumatoid arthritis (RA) and other connective tissues diseases This study explored the clinical value of collagen triple helix repeat containing-1 (CTHRC1) and 14-3-3η protein, compared to routine markers, in the diagnosis of RA.Methods: We recruited 103 RA patients, 105 non-RA patients (osteoarthritis, ankylosing spondylitis, systemic lupus erythematosus) and 59 healthy controls. CTHRC1, 14-3-3η, anti-cyclic citrullinated peptide antibody (anti-CCP), anti-mutated citrullinated vimentin antibody (anti-MCV), rheumatoid factor and erythrocyte sedimentation rate (ESR) levels were measured, and their diagnostic value for RA evaluated and compared.Results: All laboratory indices were elevated in RA (P < 0.05). Of these, anti-MCV had the highest sensitivity (86.4%) and anti-CCP the highest specificity (94.5%). The areas under the curve (AUC) of CTHRC1, 14-3-3η, anti-CCP, anti-MCV, rheumatoid factor and ESR were 0.84, 0.81, 0.89, 0.91, 0.85 and 0.77 respectively (all P < 0.01). Anti-CCP and anti-MCV were the most valuable in the diagnosis of RA. The combination of anti-CCP and anti-MCV had the maximum Youden index, followed by the combination of anti-CCP and 14-3-3η. Binary logistic regression analysis showed that 14-3-3η had the largest odds ratio value (95% CI) at 5.1 (2.1-12.5) for RA.Conclusion: CTHRC1 and 14-3-3η are promising serological indicators of RA, and when combined with anti-CCP, anti-MCV and ESR, can improve the diagnosis of this disease.","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 2","pages":"67-71"},"PeriodicalIF":1.9,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1810400","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38288818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Epidermal growth factor +61A/G (rs4444903) promoter polymorphism and serum levels are linked to idiopathic male infertility. 表皮生长因子+61A/G (rs4444903)启动子多态性和血清水平与特发性男性不育症有关

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2021-04-01 Epub Date: 2020-06-11 DOI: 10.1080/09674845.2020.1774034

M Aminmalek, F Mashayekhi, Z Salehi

引用次数: 2

Next-generation sequencing-based clinical metagenomics identifies Prevotella pleuritidis in a diabetic adolescent with large parapneumonic effusion and negative growth of pleural fluid culture: a case report. 基于新一代测序的临床宏基因组学在糖尿病青少年中发现了大量肺旁积液和胸膜液培养阴性生长的胸膜炎普雷沃氏菌:一个病例报告。

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2021-04-01 Epub Date: 2021-01-13 DOI: 10.1080/09674845.2020.1827846

T Galliguez, P Y Tsou, A Cabrera, J Fergie

引用次数: 4

Association of hTERT SNP (rs2736100) with implantation failure after in vitro fertilization and embryo transfer (IVF-ET). hTERT SNP (rs2736100)与体外受精和胚胎移植(IVF-ET)后着床失败的关联

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2021-01-01 Epub Date: 2020-06-11 DOI: 10.1080/09674845.2020.1769332

S Tajalli, F Mashayekhi, Z Salehi, S Arefi, S T Sasani

引用次数: 2

OX40 genetic variations in patients with breast cancer: a case-control study. 乳腺癌患者的OX40基因变异:一项病例对照研究

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2021-01-01 Epub Date: 2020-11-18 DOI: 10.1080/09674845.2020.1776587

Z Faghih, E Taherifard, A Daneshmand, A Talei, N Erfani

引用次数: 2

Fine structure of the human retina defined by confocal microscopic immunohistochemistry. 用免疫组织化学共聚焦显微镜观察人视网膜的精细结构。

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2021-01-01 Epub Date: 2020-07-17 DOI: 10.1080/09674845.2020.1776586

R Zhang, X Zhang, F Hu, J Wu

{"title":"Fine structure of the human retina defined by confocal microscopic immunohistochemistry.","authors":"R Zhang, X Zhang, F Hu, J Wu","doi":"10.1080/09674845.2020.1776586","DOIUrl":"https://doi.org/10.1080/09674845.2020.1776586","url":null,"abstract":"Introduction: Research in to the pathophysiology of the complex layers of retinal and sub-retinal cells is hampered by inadequate recognition of particular cells and tissues. A comprehensive panel of antibodies recognising retinal tissues is lacking. Our purpose was to determine the value of a panel of antibodies labelling various cells in the human retina.Method: Five groups of antibodies labelled frozen sections of retinas: (1) protein kinase C-α, Glutamine Synthetase (GS) and ionized calcium-binding adapter molecule 1 (Iba1); (2) Parvalbumin, Calretinin and glial fibrillary acidic protein (GFAP); (3) Thy1, GS and Iba1; (4) Rhodopsin, GS and Iba1; and (5) Brn3a, Rhodopsin and protein kinase C-α. The distribution of these antigens were determined by confocal microscopy and calculated grey value of each antibody in each layer of the retina by Image J.Results: Different antibodies determined certain retinal layers. Thy 1 is a good determinant of the ganglion cell layer, whilst GS is present in all layers except the photoreceptor layer. Brn3a is specific for the ganglion cell layer whilst parvalbumin marks the ganglion cell layer and the outer plexiform layer. Rhodopsin strongly marks the photoreceptor layer, but this is also marked weakly by GFAP.Conclusion: The multiple labelling of human retinal cells brings further understanding of the biological characteristics and functions of these cells, and provides a theoretical basis for their possible role in diseases. In the growing field of human retina research, our data may provide a point of reference for future studies of the human retina.","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 1","pages":"28-34"},"PeriodicalIF":1.9,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1776586","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38014617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Association between SLCO1B1 polymorphism distribution frequency and blood lipid level in Chinese adults. 中国成人SLCO1B1多态性分布频率与血脂水平的关系

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2021-01-01 Epub Date: 2020-07-22 DOI: 10.1080/09674845.2020.1785692

Q Mo, S Huang, J Ma, J Zhang, R Su, Q Deng

{"title":"Association between SLCO1B1 polymorphism distribution frequency and blood lipid level in Chinese adults.","authors":"Q Mo, S Huang, J Ma, J Zhang, R Su, Q Deng","doi":"10.1080/09674845.2020.1785692","DOIUrl":"https://doi.org/10.1080/09674845.2020.1785692","url":null,"abstract":"Background: The variation of serum lipid levels can be part-related to certain genes. One such gene, SLCO1B1, encodes a transporter that may have a role in lipid metabolism. We hypothesised that differences in certain SLCO1B1 genotypes are related to levels of serum lipids.Materials and methods: We recruited 636 subjects who were genotyped for SLCO1B1 variants *1a, *1b, *5 and *15. Routine liver function tests, renal function tests and routine lipid indices were measured by standard techniques.Results: The most frequent genotypes were *1b/*1b (29.3%), *1b/*15 (27.5%), *1a/*1b (21.1%), *1a/*15 and *1b/*5 (10.2%) and *1a/*1a (8.5%). There were significant differences in levels of triglycerides and HDL in the four SLCO1B1 genotypes *1a/*1a, *1b/*1b, *1a/*1b and *1b/*15 (all p < 0.05).Conclusion: The genotypes *1a/*1a and *1a/*1b indicate a high risk of cardiovascular disease, while the *1b/*1b group may have a relatively low risk. SLCO1B1 may be involved in the metabolism of triglycerides and HDL. We have provided a tool for identifying potentially high-risk groups that could be helpful for early diagnosis and prevention, individualized drug therapy and even gene therapy.","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 1","pages":"23-27"},"PeriodicalIF":1.9,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1785692","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38098518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The effects of human immunodeficiency virus, human papillomavirus, herpes simplex virus-1 and -2, human herpesvirus-6 and -8, cytomegalovirus, and hepatitis B and C virus on female fertility and pregnancy. 人类免疫缺陷病毒、人类乳头瘤病毒、单纯疱疹病毒-1和-2、人类疱疹病毒-6和-8、巨细胞病毒、乙型和丙型肝炎病毒对女性生育和妊娠的影响。

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2021-01-01 Epub Date: 2020-09-07 DOI: 10.1080/09674845.2020.1803540

M Farsimadan, M Motamedifar

{"title":"The effects of human immunodeficiency virus, human papillomavirus, herpes simplex virus-1 and -2, human herpesvirus-6 and -8, cytomegalovirus, and hepatitis B and C virus on female fertility and pregnancy.","authors":"M Farsimadan, M Motamedifar","doi":"10.1080/09674845.2020.1803540","DOIUrl":"https://doi.org/10.1080/09674845.2020.1803540","url":null,"abstract":"Female infertility may be defined as a woman of reproductive age being unable to become pregnant after a year of regular unprotected sexual intercourse. Social, genetic, endocrine, physiological, and psychological factors as well as lifestyle habits (i.e., smoking and alcohol consumption), either alone or in combination with male factors, are major causes. However, approximately 15-30% of cases of female infertility remain unexplained. Numerous investigations have also indicated that microbiomes play an important role in human reproduction. All parts of the female reproductive system may be influenced by infectious and pathological agents, especially viruses, and these may interfere with reproductive function and so are risk factors for infertility, although in many cases an exact role is unclear. We present an overview of the impact of common viral infections on female reproduction, searching Medline, PubMed, Scopus, and Google scholar databases for potentially relevant studies of viruses known to have a potential effect. Human immunodeficiency virus (HIV), herpes simplex virus (HSV) and human herpesvirus (HHV) increase infertility rates whilst human papillomavirus (HPV), cytomegalovirus (CMV), and hepatitis B and C virus (HBV, HCV) infections mostly lead to higher abortion and miscarriage rates. Moreover, HPV infection is linked to increased tubal infertility, endometriosis, and pelvic inflammatory disease. HPV was the most frequently observed infection and with lower pregnancy rate and foetal death in women undergoing IVF treatments. Assisted reproductive treatment could be a safe and effective approach for HIV and HBV infected women.","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 1","pages":"1-11"},"PeriodicalIF":1.9,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1803540","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38213435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Toll-like receptor 7 mRNA is reduced in hepatitis C-based liver cirrhosis and hepatocellular carcinoma, out-performs alpha-fetoprotein levels, and with age and serum aspartate aminotransferase is a new diagnostic index. toll样受体7mrna在丙型肝炎肝硬化和肝细胞癌中降低，表现优于甲胎蛋白水平，随年龄和血清天冬氨酸转氨酶是一种新的诊断指标。

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2021-01-01 Epub Date: 2020-07-20 DOI: 10.1080/09674845.2020.1778842

A A Mohamed, D Omran, S El-Feky, H Darwish, Ael Kassas, A Farouk, O Ezzat, S M Abdo, F E Zahran, A El-Demery, M M Omran

{"title":"Toll-like receptor 7 mRNA is reduced in hepatitis C-based liver cirrhosis and hepatocellular carcinoma, out-performs alpha-fetoprotein levels, and with age and serum aspartate aminotransferase is a new diagnostic index.","authors":"A A Mohamed, D Omran, S El-Feky, H Darwish, Ael Kassas, A Farouk, O Ezzat, S M Abdo, F E Zahran, A El-Demery, M M Omran","doi":"10.1080/09674845.2020.1778842","DOIUrl":"https://doi.org/10.1080/09674845.2020.1778842","url":null,"abstract":"Background: Hepatitis B and C viruses are leading causes of liver cirrhosis and hepatocellular carcinoma (HCC). Toll-like receptor 7 (TLR-7) has been implicated in the pathogenesis of HCC linked to hepatitis B. We hypothesised a role of leukocyte TLR-7 mRNA in hepatitis C related liver cirrhosis and HCC, using alpha-fetoprotein (AFP) and liver function tests as comparators.Methods: We recruited 102 patients with HCV-related HCC, 97 with HCV-related liver cirrhosis and 60 healthy controls. Quantification of TLR-7 mRNA was performed using real-time PCR, AFP and routine LFTs by standard techniques.Results: TLR-7 mRNA levels were significantly lower in HCC patients compared to cirrhotic patients and lower again in healthy controls (p < 0.001 for trend). In multivariate analysis, age, aspartate transaminase (AST), AFP, and TLR-7 mRNA were significant predictors of HCC. The ROCC/AUC for age, AST and TLR-7 mRNA were all between 0.64 and 0.78 (all P < 0.01), but for AFP was 0.57 (95% CI 0.48-0.65, P = 0.09). We derived an index score using age, AST and TLR-7 mRNA for the diagnosis of HCC. The ROCC/AUC for the index was superior to all three root indices in the prediction of HCC. The index linked significantly with the Tokyo and Vienna liver cancer staging systems, but not with those of the CLIP and Okuda systems, in distinguishing HCC from liver cirrhosis.Conclusion: The combination of TLR-7 mRNA levels with age and AST improves the performance of TLR-7 in HCC diagnosis, out-performs alpha-fetoprotein and predicts early HCC.","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 1","pages":"18-22"},"PeriodicalIF":1.9,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1778842","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38074201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5