British Journal of Biomedical Science最新文献

{"title":"Epidermal growth factor +61A/G (rs4444903) promoter polymorphism and serum levels are linked to idiopathic male infertility.","authors":"M Aminmalek, F Mashayekhi, Z Salehi","doi":"10.1080/09674845.2020.1774034","DOIUrl":"https://doi.org/10.1080/09674845.2020.1774034","url":null,"abstract":"","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 2","pages":"92-94"},"PeriodicalIF":1.9,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1774034","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37971157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Next-generation sequencing-based clinical metagenomics identifies <i>Prevotella pleuritidis</i> in a diabetic adolescent with large parapneumonic effusion and negative growth of pleural fluid culture: a case report.","authors":"T Galliguez,&nbsp;P Y Tsou,&nbsp;A Cabrera,&nbsp;J Fergie","doi":"10.1080/09674845.2020.1827846","DOIUrl":"https://doi.org/10.1080/09674845.2020.1827846","url":null,"abstract":"<p><p>We report a 12-year-old diabetic boy with a right-sided parapneumonic effusion and pneumonia who failed initial empirical antibiotics. <i>Prevotella pleuritidis</i> was identified from the pleural fluid using next-generation sequencing-based clinical metagenomics with cultures of pleural fluid and blood resulting negative. The patient responded well to intravenous meropenem followed by oral metronidazole.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 2","pages":"101-105"},"PeriodicalIF":1.9,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1827846","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38435512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of <i>hTERT</i> SNP (rs2736100) with implantation failure after <i>in vitro</i> fertilization and embryo transfer (IVF-ET).","authors":"S Tajalli,&nbsp;F Mashayekhi,&nbsp;Z Salehi,&nbsp;S Arefi,&nbsp;S T Sasani","doi":"10.1080/09674845.2020.1769332","DOIUrl":"https://doi.org/10.1080/09674845.2020.1769332","url":null,"abstract":"","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 1","pages":"41-43"},"PeriodicalIF":1.9,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1769332","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37929311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OX40 genetic variations in patients with breast cancer: a case-control study.","authors":"Z Faghih,&nbsp;E Taherifard,&nbsp;A Daneshmand,&nbsp;A Talei,&nbsp;N Erfani","doi":"10.1080/09674845.2020.1776587","DOIUrl":"https://doi.org/10.1080/09674845.2020.1776587","url":null,"abstract":"","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 1","pages":"44-46"},"PeriodicalIF":1.9,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1776587","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38372575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fine structure of the human retina defined by confocal microscopic immunohistochemistry.","authors":"R Zhang,&nbsp;X Zhang,&nbsp;F Hu,&nbsp;J Wu","doi":"10.1080/09674845.2020.1776586","DOIUrl":"https://doi.org/10.1080/09674845.2020.1776586","url":null,"abstract":"<p><strong>Introduction: </strong>Research in to the pathophysiology of the complex layers of retinal and sub-retinal cells is hampered by inadequate recognition of particular cells and tissues. A comprehensive panel of antibodies recognising retinal tissues is lacking. Our purpose was to determine the value of a panel of antibodies labelling various cells in the human retina.</p><p><strong>Method: </strong>Five groups of antibodies labelled frozen sections of retinas: (1) protein kinase C-α, Glutamine Synthetase (GS) and ionized calcium-binding adapter molecule 1 (Iba1); (2) Parvalbumin, Calretinin and glial fibrillary acidic protein (GFAP); (3) Thy1, GS and Iba1; (4) Rhodopsin, GS and Iba1; and (5) Brn3a, Rhodopsin and protein kinase C-α. The distribution of these antigens were determined by confocal microscopy and calculated grey value of each antibody in each layer of the retina by Image J.</p><p><strong>Results: </strong>Different antibodies determined certain retinal layers. Thy 1 is a good determinant of the ganglion cell layer, whilst GS is present in all layers except the photoreceptor layer. Brn3a is specific for the ganglion cell layer whilst parvalbumin marks the ganglion cell layer and the outer plexiform layer. Rhodopsin strongly marks the photoreceptor layer, but this is also marked weakly by GFAP.</p><p><strong>Conclusion: </strong>The multiple labelling of human retinal cells brings further understanding of the biological characteristics and functions of these cells, and provides a theoretical basis for their possible role in diseases. In the growing field of human retina research, our data may provide a point of reference for future studies of the human retina.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 1","pages":"28-34"},"PeriodicalIF":1.9,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1776586","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38014617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between <i>SLCO1B1</i> polymorphism distribution frequency and blood lipid level in Chinese adults.","authors":"Q Mo,&nbsp;S Huang,&nbsp;J Ma,&nbsp;J Zhang,&nbsp;R Su,&nbsp;Q Deng","doi":"10.1080/09674845.2020.1785692","DOIUrl":"https://doi.org/10.1080/09674845.2020.1785692","url":null,"abstract":"<p><strong>Background: </strong>The variation of serum lipid levels can be part-related to certain genes. One such gene, <i>SLCO1B1</i>, encodes a transporter that may have a role in lipid metabolism. We hypothesised that differences in certain <i>SLCO1B1</i> genotypes are related to levels of serum lipids.</p><p><strong>Materials and methods: </strong>We recruited 636 subjects who were genotyped for <i>SLCO1B1</i> variants *1a, *1b, *5 and *15. Routine liver function tests, renal function tests and routine lipid indices were measured by standard techniques.</p><p><strong>Results: </strong>The most frequent genotypes were *1b/*1b (29.3%), *1b/*15 (27.5%), *1a/*1b (21.1%), *1a/*15 and *1b/*5 (10.2%) and *1a/*1a (8.5%). There were significant differences in levels of triglycerides and HDL in the four <i>SLCO1B1</i> genotypes *1a/*1a, *1b/*1b, *1a/*1b and *1b/*15 (all p < 0.05).</p><p><strong>Conclusion: </strong>The genotypes *1a/*1a and *1a/*1b indicate a high risk of cardiovascular disease, while the *1b/*1b group may have a relatively low risk. <i>SLCO1B1</i> may be involved in the metabolism of triglycerides and HDL. We have provided a tool for identifying potentially high-risk groups that could be helpful for early diagnosis and prevention, individualized drug therapy and even gene therapy.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 1","pages":"23-27"},"PeriodicalIF":1.9,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1785692","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38098518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of human immunodeficiency virus, human papillomavirus, herpes simplex virus-1 and -2, human herpesvirus-6 and -8, cytomegalovirus, and hepatitis B and C virus on female fertility and pregnancy.","authors":"M Farsimadan,&nbsp;M Motamedifar","doi":"10.1080/09674845.2020.1803540","DOIUrl":"https://doi.org/10.1080/09674845.2020.1803540","url":null,"abstract":"<p><p>Female infertility may be defined as a woman of reproductive age being unable to become pregnant after a year of regular unprotected sexual intercourse. Social, genetic, endocrine, physiological, and psychological factors as well as lifestyle habits (i.e., smoking and alcohol consumption), either alone or in combination with male factors, are major causes. However, approximately 15-30% of cases of female infertility remain unexplained. Numerous investigations have also indicated that microbiomes play an important role in human reproduction. All parts of the female reproductive system may be influenced by infectious and pathological agents, especially viruses, and these may interfere with reproductive function and so are risk factors for infertility, although in many cases an exact role is unclear. We present an overview of the impact of common viral infections on female reproduction, searching Medline, PubMed, Scopus, and Google scholar databases for potentially relevant studies of viruses known to have a potential effect. Human immunodeficiency virus (HIV), herpes simplex virus (HSV) and human herpesvirus (HHV) increase infertility rates whilst human papillomavirus (HPV), cytomegalovirus (CMV), and hepatitis B and C virus (HBV, HCV) infections mostly lead to higher abortion and miscarriage rates. Moreover, HPV infection is linked to increased tubal infertility, endometriosis, and pelvic inflammatory disease. HPV was the most frequently observed infection and with lower pregnancy rate and foetal death in women undergoing IVF treatments. Assisted reproductive treatment could be a safe and effective approach for HIV and HBV infected women.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 1","pages":"1-11"},"PeriodicalIF":1.9,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1803540","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38213435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toll-like receptor 7 mRNA is reduced in hepatitis C-based liver cirrhosis and hepatocellular carcinoma, out-performs alpha-fetoprotein levels, and with age and serum aspartate aminotransferase is a new diagnostic index.","authors":"A A Mohamed,&nbsp;D Omran,&nbsp;S El-Feky,&nbsp;H Darwish,&nbsp;Ael Kassas,&nbsp;A Farouk,&nbsp;O Ezzat,&nbsp;S M Abdo,&nbsp;F E Zahran,&nbsp;A El-Demery,&nbsp;M M Omran","doi":"10.1080/09674845.2020.1778842","DOIUrl":"https://doi.org/10.1080/09674845.2020.1778842","url":null,"abstract":"<p><strong>Background: </strong>Hepatitis B and C viruses are leading causes of liver cirrhosis and hepatocellular carcinoma (HCC). Toll-like receptor 7 (TLR-7) has been implicated in the pathogenesis of HCC linked to hepatitis B. We hypothesised a role of leukocyte TLR-7 mRNA in hepatitis C related liver cirrhosis and HCC, using alpha-fetoprotein (AFP) and liver function tests as comparators.</p><p><strong>Methods: </strong>We recruited 102 patients with HCV-related HCC, 97 with HCV-related liver cirrhosis and 60 healthy controls. Quantification of TLR-7 mRNA was performed using real-time PCR, AFP and routine LFTs by standard techniques.</p><p><strong>Results: </strong>TLR-7 mRNA levels were significantly lower in HCC patients compared to cirrhotic patients and lower again in healthy controls (p < 0.001 for trend). In multivariate analysis, age, aspartate transaminase (AST), AFP, and TLR-7 mRNA were significant predictors of HCC. The ROCC/AUC for age, AST and TLR-7 mRNA were all between 0.64 and 0.78 (all <i>P</i> < 0.01), but for AFP was 0.57 (95% CI 0.48-0.65, <i>P</i> = 0.09). We derived an index score using age, AST and TLR-7 mRNA for the diagnosis of HCC. The ROCC/AUC for the index was superior to all three root indices in the prediction of HCC. The index linked significantly with the Tokyo and Vienna liver cancer staging systems, but not with those of the CLIP and Okuda systems, in distinguishing HCC from liver cirrhosis.</p><p><strong>Conclusion: </strong>The combination of TLR-7 mRNA levels with age and AST improves the performance of TLR-7 in HCC diagnosis, out-performs alpha-fetoprotein and predicts early HCC.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 1","pages":"18-22"},"PeriodicalIF":1.9,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1778842","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38074201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of laparoscopic gastric bypass <i>vs</i> laparoscopic sleeve gastrectomy in treating obesity combined with type-2 diabetes.","authors":"Y Yan,&nbsp;F Wang,&nbsp;H Chen,&nbsp;X Zhao,&nbsp;D Yin,&nbsp;Y Hui,&nbsp;N Ma,&nbsp;C Yang,&nbsp;Z Zheng,&nbsp;T Zhang,&nbsp;N Xu,&nbsp;G Wang","doi":"10.1080/09674845.2020.1798578","DOIUrl":"https://doi.org/10.1080/09674845.2020.1798578","url":null,"abstract":"<p><strong>Objective: </strong>This prospective study compared the efficacy and safety of laparoscopic gastric bypass and laparoscopic sleeve gastrectomy in treating overweight and obese patients with BMI>28 kg/m² and type-2 diabetes.</p><p><strong>Methods: </strong>Patients were randomized into a gastric bypass group (n = 77) or a gastrectomy group (n = 80). The surgery time, intraoperative blood loss, recovery time, and hospitalization time were collected. BMI, waistline, hipline, C-peptide level, insulin resistance index (HOMA-IR), and their blood and lipid profile were also measured.</p><p><strong>Results: </strong>Surgery time and blood loss were significantly higher in the gastrectomy group, when compared to the gastric bypass group (<i>P </i>< 0.05). In both groups, the levels of BMI, waist circumference and hip circumference (but not their ratio) gradually and significantly decreased after surgery compared with baseline (<i>P </i>< 0.05), and no significant difference was found between these two groups. The C-peptide level, HOMA-IR, fasting blood glucose, 2-hour postprandial blood glucose and glycosylated haemoglobin gradually and significantly decreased after surgery compared with the values before treatment (<i>P </i>< 0.05). The levels of total cholesterol, triglyceride, LDL, and monocyte chemoattractant protein-1 were also lower after surgery in both groups, while HDL and glucagon-like peptide-1 were significantly higher after surgery compared with the values before treatment (<i>P </i>< 0.05). However, no significant difference was found between these two groups of patients.</p><p><strong>Conclusion: </strong>Both laparoscopic gastric bypass and laparoscopic sleeve gastrectomy improved the BMI and diabetic conditions of overweight/obese diabetics, while laparoscopic sleeve gastrectomy had a shorter surgical time and less blood loss.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 1","pages":"35-40"},"PeriodicalIF":1.9,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1798578","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38182151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circulating micro RNA-223 and angiopoietin-like protein 8 as biomarkers of gestational diabetes mellitus.","authors":"A Abdeltawab,&nbsp;M E Zaki,&nbsp;Y Abdeldayem,&nbsp;A A Mohamed,&nbsp;S M Zaied","doi":"10.1080/09674845.2020.1764211","DOIUrl":"https://doi.org/10.1080/09674845.2020.1764211","url":null,"abstract":"<p><strong>Background: </strong>Gestational diabetes mellitus (GDM) is a serious health problem associated with both foetal and maternal complications. New biomarkers that can predict or help in the early diagnosis of GDM are needed to minimize the hazards of hyperglycaemia in pregnant women and their offspring. We hypothesised a link between levels of microRNA-223 (miRNA-223) and Angiopoietin-Like Protein 8 (ANGPTL8) and GDM.</p><p><strong>Materials and methods: </strong>The study included 109 patients with confirmed early diagnosed GDM and 103 healthy control pregnant women in their second or third trimester. miRNA-223 and ANGPTL8 blood levels were assessed by real-time RT-PCR and sandwich ELISA, respectively, laboratory markers by standard methods.</p><p><strong>Results: </strong>There was a significant increase in mean [SD] miRNA-223 and ANGPTL8 in GDM (0.31 [0.06] relative units) and (692 [199] pg/ml), respectively, in the GDM women compared to healthy pregnant women (0.17[0.05] relative units) and (261 [127] pg/ml), respectively, <i>P</i> <i> < 0.001</i>. miRNA-223 and ANGPTL8 correlated significantly with each other (<i>r </i>= 0.38, <i>P </i> <i>< 0.001</i>) and with fasting, 1-h and 2-h postprandial blood glucose levels (all <i>P</i> <i>≤ 0.002</i>) HbA1 c (<i>P</i> <i> < 0.025</i>), total cholesterol (<i>P < 0.01</i>), LDL-C and triglycerides (both <i>P ≤ 0.005</i>). The ROC area under curve (AUC) (95%CI) was 0.94 (0.91-0.97) for ANGPTL8, 0.92 (0.88-0.96) for miRNA-223 and 0.97 (0.95 - 0.99) for their combination.</p><p><strong>Conclusions: </strong>These findings support the hypothesis of involvement of both miRNA-223 and ANGPTL8 in the pathogenesis of GDM. The difference between levels in GDM patients and in control pregnant women indicates potential use for early diagnosis or prediction of GDM.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 1","pages":"12-17"},"PeriodicalIF":1.9,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1764211","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37948241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}