British Journal of Biomedical Science最新文献

{"title":"Toll-like receptor 7 mRNA is reduced in hepatitis C-based liver cirrhosis and hepatocellular carcinoma, out-performs alpha-fetoprotein levels, and with age and serum aspartate aminotransferase is a new diagnostic index.","authors":"A A Mohamed,&nbsp;D Omran,&nbsp;S El-Feky,&nbsp;H Darwish,&nbsp;Ael Kassas,&nbsp;A Farouk,&nbsp;O Ezzat,&nbsp;S M Abdo,&nbsp;F E Zahran,&nbsp;A El-Demery,&nbsp;M M Omran","doi":"10.1080/09674845.2020.1778842","DOIUrl":"https://doi.org/10.1080/09674845.2020.1778842","url":null,"abstract":"<p><strong>Background: </strong>Hepatitis B and C viruses are leading causes of liver cirrhosis and hepatocellular carcinoma (HCC). Toll-like receptor 7 (TLR-7) has been implicated in the pathogenesis of HCC linked to hepatitis B. We hypothesised a role of leukocyte TLR-7 mRNA in hepatitis C related liver cirrhosis and HCC, using alpha-fetoprotein (AFP) and liver function tests as comparators.</p><p><strong>Methods: </strong>We recruited 102 patients with HCV-related HCC, 97 with HCV-related liver cirrhosis and 60 healthy controls. Quantification of TLR-7 mRNA was performed using real-time PCR, AFP and routine LFTs by standard techniques.</p><p><strong>Results: </strong>TLR-7 mRNA levels were significantly lower in HCC patients compared to cirrhotic patients and lower again in healthy controls (p < 0.001 for trend). In multivariate analysis, age, aspartate transaminase (AST), AFP, and TLR-7 mRNA were significant predictors of HCC. The ROCC/AUC for age, AST and TLR-7 mRNA were all between 0.64 and 0.78 (all <i>P</i> < 0.01), but for AFP was 0.57 (95% CI 0.48-0.65, <i>P</i> = 0.09). We derived an index score using age, AST and TLR-7 mRNA for the diagnosis of HCC. The ROCC/AUC for the index was superior to all three root indices in the prediction of HCC. The index linked significantly with the Tokyo and Vienna liver cancer staging systems, but not with those of the CLIP and Okuda systems, in distinguishing HCC from liver cirrhosis.</p><p><strong>Conclusion: </strong>The combination of TLR-7 mRNA levels with age and AST improves the performance of TLR-7 in HCC diagnosis, out-performs alpha-fetoprotein and predicts early HCC.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 1","pages":"18-22"},"PeriodicalIF":1.9,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1778842","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38074201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of laparoscopic gastric bypass <i>vs</i> laparoscopic sleeve gastrectomy in treating obesity combined with type-2 diabetes.","authors":"Y Yan,&nbsp;F Wang,&nbsp;H Chen,&nbsp;X Zhao,&nbsp;D Yin,&nbsp;Y Hui,&nbsp;N Ma,&nbsp;C Yang,&nbsp;Z Zheng,&nbsp;T Zhang,&nbsp;N Xu,&nbsp;G Wang","doi":"10.1080/09674845.2020.1798578","DOIUrl":"https://doi.org/10.1080/09674845.2020.1798578","url":null,"abstract":"<p><strong>Objective: </strong>This prospective study compared the efficacy and safety of laparoscopic gastric bypass and laparoscopic sleeve gastrectomy in treating overweight and obese patients with BMI>28 kg/m² and type-2 diabetes.</p><p><strong>Methods: </strong>Patients were randomized into a gastric bypass group (n = 77) or a gastrectomy group (n = 80). The surgery time, intraoperative blood loss, recovery time, and hospitalization time were collected. BMI, waistline, hipline, C-peptide level, insulin resistance index (HOMA-IR), and their blood and lipid profile were also measured.</p><p><strong>Results: </strong>Surgery time and blood loss were significantly higher in the gastrectomy group, when compared to the gastric bypass group (<i>P </i>< 0.05). In both groups, the levels of BMI, waist circumference and hip circumference (but not their ratio) gradually and significantly decreased after surgery compared with baseline (<i>P </i>< 0.05), and no significant difference was found between these two groups. The C-peptide level, HOMA-IR, fasting blood glucose, 2-hour postprandial blood glucose and glycosylated haemoglobin gradually and significantly decreased after surgery compared with the values before treatment (<i>P </i>< 0.05). The levels of total cholesterol, triglyceride, LDL, and monocyte chemoattractant protein-1 were also lower after surgery in both groups, while HDL and glucagon-like peptide-1 were significantly higher after surgery compared with the values before treatment (<i>P </i>< 0.05). However, no significant difference was found between these two groups of patients.</p><p><strong>Conclusion: </strong>Both laparoscopic gastric bypass and laparoscopic sleeve gastrectomy improved the BMI and diabetic conditions of overweight/obese diabetics, while laparoscopic sleeve gastrectomy had a shorter surgical time and less blood loss.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 1","pages":"35-40"},"PeriodicalIF":1.9,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1798578","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38182151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circulating micro RNA-223 and angiopoietin-like protein 8 as biomarkers of gestational diabetes mellitus.","authors":"A Abdeltawab,&nbsp;M E Zaki,&nbsp;Y Abdeldayem,&nbsp;A A Mohamed,&nbsp;S M Zaied","doi":"10.1080/09674845.2020.1764211","DOIUrl":"https://doi.org/10.1080/09674845.2020.1764211","url":null,"abstract":"<p><strong>Background: </strong>Gestational diabetes mellitus (GDM) is a serious health problem associated with both foetal and maternal complications. New biomarkers that can predict or help in the early diagnosis of GDM are needed to minimize the hazards of hyperglycaemia in pregnant women and their offspring. We hypothesised a link between levels of microRNA-223 (miRNA-223) and Angiopoietin-Like Protein 8 (ANGPTL8) and GDM.</p><p><strong>Materials and methods: </strong>The study included 109 patients with confirmed early diagnosed GDM and 103 healthy control pregnant women in their second or third trimester. miRNA-223 and ANGPTL8 blood levels were assessed by real-time RT-PCR and sandwich ELISA, respectively, laboratory markers by standard methods.</p><p><strong>Results: </strong>There was a significant increase in mean [SD] miRNA-223 and ANGPTL8 in GDM (0.31 [0.06] relative units) and (692 [199] pg/ml), respectively, in the GDM women compared to healthy pregnant women (0.17[0.05] relative units) and (261 [127] pg/ml), respectively, <i>P</i> <i> < 0.001</i>. miRNA-223 and ANGPTL8 correlated significantly with each other (<i>r </i>= 0.38, <i>P </i> <i>< 0.001</i>) and with fasting, 1-h and 2-h postprandial blood glucose levels (all <i>P</i> <i>≤ 0.002</i>) HbA1 c (<i>P</i> <i> < 0.025</i>), total cholesterol (<i>P < 0.01</i>), LDL-C and triglycerides (both <i>P ≤ 0.005</i>). The ROC area under curve (AUC) (95%CI) was 0.94 (0.91-0.97) for ANGPTL8, 0.92 (0.88-0.96) for miRNA-223 and 0.97 (0.95 - 0.99) for their combination.</p><p><strong>Conclusions: </strong>These findings support the hypothesis of involvement of both miRNA-223 and ANGPTL8 in the pathogenesis of GDM. The difference between levels in GDM patients and in control pregnant women indicates potential use for early diagnosis or prediction of GDM.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 1","pages":"12-17"},"PeriodicalIF":1.9,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1764211","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37948241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atypical and novel presentations of Coronavirus Disease 2019: a case series of three children.","authors":"M S Ekbatani,&nbsp;S A Hassani,&nbsp;L Tahernia,&nbsp;B Yaghmaei,&nbsp;S Mahmoudi,&nbsp;A Navaeian,&nbsp;M Rostamyan,&nbsp;F Zamani,&nbsp;S Mamishi","doi":"10.1080/09674845.2020.1785102","DOIUrl":"https://doi.org/10.1080/09674845.2020.1785102","url":null,"abstract":"<p><p>Typical presentations of Coronavirus Disease 2019 (Covid-19) including respiratory symptoms (cough, respiratory distress and hypoxia), fever and dyspnoea are considered main symptoms in adults, but atypical presentation in children could be a diagnostic challenge. We report three children whose initial presentation was gastrointestinal, and in whom Covid-19 infection was found, concluding that cases of acute appendicitis, mesenteric adenitis and flank tenderness may mask an infection with this virus, and should therefore be investigated.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 1","pages":"47-52"},"PeriodicalIF":1.9,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1785102","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38055998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic value of bone marrow MUC4 expression in acute myeloid leukaemia.","authors":"A S Abdelhady,&nbsp;F F Abdel Hamid,&nbsp;N M Hassan,&nbsp;D M Ibrahim","doi":"10.1080/09674845.2020.1754583","DOIUrl":"https://doi.org/10.1080/09674845.2020.1754583","url":null,"abstract":"<p><p><b>Background:</b> Aberrant expression of mucin-4 (MUC4) is present in a variety of solid cancers, but the expression pattern of <i>MUC4</i> and its clinical relevance in acute myeloid leukaemia (AML) is unknown. We aimed to evaluate the expression level of <i>MUC4</i> and explore its prognostic value in newly diagnosed adult patients with AML. <b>Methods:</b> Bone marrow from 70 AML patients and 26 healthy donors was obtained. <i>MUC4</i> levels were quantified by quantitative real-time PCR. Routine blood indices were measured by standard techniques. <b>Results:</b> Bone marrow <i>MUC4</i> expression levels were significantly elevated in AML patients compared to controls at median (range) 2.77 (0.7-16.6) and 1.14 (0.5-1.99) respectively (p = 0.005). Moreover, lower <i>MUC4</i> expression was strongly associated with persistent remission (p = 0.001) while higher <i>MUC4</i> levels were associated with worse overall as well as disease-free survival (p = 0.011 and p = 0.006, respectively). Thus, its level may act as an indicator of disease progression. High <i>MUC4</i> expression was identified as an independent prognostic predictor for both overall survival and disease-free survival. <b>Conclusion:</b> <i>MUC4</i> over-expression is an independent predictor of a poor prognosis in AML patients.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"77 4","pages":"202-207"},"PeriodicalIF":1.9,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1754583","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37818782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationships between maternal and placental polymorphisms of miR-196a2 and miRNA-499 genes and preeclampsia.","authors":"M Asadi-Tarani,&nbsp;M Saravani,&nbsp;B Teimoori,&nbsp;M Ghasemi,&nbsp;S Salimi","doi":"10.1080/09674845.2020.1769331","DOIUrl":"https://doi.org/10.1080/09674845.2020.1769331","url":null,"abstract":"<p><p><b>Background</b>: miRNAs are small non-coding RNAs with potential roles in the complications of pregnancy. We hypothesised links between polymorphisms in miRNA-196a2 and miRNA-499 in maternal blood and the placentas of patients with preeclampsia. <b>Methods</b>: The blood of 315 women with preeclampsia and 317 controls and the placentas of 103 PE and 133 healthy women were collected. The genotyping of both polymorphisms was performed by PCR-RFLP. <b>Results</b>: The maternal blood rs11614913 was unrelated to preeclampsia in genotype and allele models, but in placental tissue, the CT (odds ratio [95% CI] 0.5 [0.3-0.9, p = 0.018) and TT (0.4 [0.2-0.9] p = 0.033) genotypes alone and together (CT+TT v CC 0.5 [0.3-0.8] p = 0.009), and the T allele (0.6 [0.4-0.9], p = 0.019) were associated with lower risk of preeclampsia. The maternal blood rs3746444 CC genotype was more frequent in preeclampsia (2.2 [1.2-3.8] p = 0.008) and the recessive model (CC v TC+TT) was also significant (1.9 [1.1-3.3], p = 0.018), as was the C allele (1.4 [1.1-1.7] p = 0.014). In placental tissue, the increase in the frequency of the CC genotype was marginally significant (2.4 [1.0-5.8] p = 0.046). The maternal or placental miRNA-196a2 rs11614913 and miRNA-499 rs3746444 polymorphisms were unrelated to the severity of preeclampsia. <b>Conclusion</b>: The placental but not maternal miRNA-196a2 rs11614913 variant could be a protective factor for preeclampsia predisposition in all models except the recessive model. The maternal/placental rs3746444 CC genotype was in association with higher preeclampsia risk.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"77 4","pages":"191-195"},"PeriodicalIF":1.9,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1769331","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38179018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relevance of <i>KISS1</i> gene polymorphisms in susceptibility to polycystic ovary syndrome and its associated endocrine and metabolic disturbances.","authors":"M H Daghestani,&nbsp;M H Daghestani,&nbsp;M Daghistani,&nbsp;K Ambreen,&nbsp;M N Almuammar,&nbsp;L M Al Neghery,&nbsp;A S Warsy","doi":"10.1080/09674845.2020.1726662","DOIUrl":"https://doi.org/10.1080/09674845.2020.1726662","url":null,"abstract":"<p><p><b>Background</b>: Variations in <i>KISS1</i> may be an emerging factor in polycystic ovary syndrome (PCOS) We hypothesised links between <i>KISS1</i> polymorphisms in PCOS and its associated endocrine and metabolic disturbances. <b>Methods</b>: The study included 104 PCOS women and 109 controls. Endocrine (kisspeptin, LH, FSH, LH-FSH ratio, oestradiol) and metabolic (cholesterol, triglycerides, HDL, LDL, insulin and glucose) parameters were measured. PCR and nucleotide sequencing were carried out to screen single nucleotide polymorphisms (SNPs) of <i>KISS1</i>. Endocrine and metabolic parameters of PCOS women were compared in the genotypes. <b>Results</b>: Three novel SNPs (rs1213704663C>G, rs1481572212T>G and rs775770652G>A) were detected in <i>KISS1</i>. Of these SNPs, the genotype and allele frequencies of rs1213704663C>G were all significantly associated p<0.001 with PCOS. The LH and oestradiol hypersecretion, and increased LH-FSH ratio of PCOS women were significantly influenced by the GG genotype of rs1213704663, but, this SNP did not influence kisspeptin levels. The other two SNPs rs1481572212T>G and rs775770652G>A exhibited no clinical significance. <b>Conclusion</b>: rs1213704663C>G variation in <i>KISS1</i> is linked to PCOS and its associated endocrine and metabolic disturbances (LH and oestradiol hypersecretion, and increased LH/FSH).</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"77 4","pages":"185-190"},"PeriodicalIF":1.9,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1726662","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37620762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel serum metabolome score for breast cancer diagnosis.","authors":"R Rashed,&nbsp;H Darwish,&nbsp;M Omran,&nbsp;A Belal,&nbsp;F Zahran","doi":"10.1080/09674845.2020.1784568","DOIUrl":"https://doi.org/10.1080/09674845.2020.1784568","url":null,"abstract":"<p><strong>Introduction: </strong>Early detection of breast cancer is important in diagnosis and treatment, and so in enhancing patient survival and reducing death rates. Because of the low diagnostic sensitivity and specificity of widely used breast cancer biomarkers such as CA15-3, we hypothesised that a panel of new metabolic markers would provide superior sensitivity and specificity for this disease.</p><p><strong>Material & methods: </strong>We recruited 120 women with malignant breast cancer, 47 with benign breast disease and 55 females as a healthy control group. Metabolites 8-hydroxy-2'-deoxyguanosine, 1-methylguanosine, and 1-methyl adenosine were detected and quantified by gas chromatography-mass spectrometry, CA15.3 by ELISA. Cut-off values of individual and combined metabolome with CA15-3 were analysed using the receiver operating characteristics curve (ROCC) to test the efficiency of the candidate metabolome in identifying breast cancer.</p><p><strong>Results: </strong>The overall linear trend of biomarkers across the groups was significant with highest levels in breast cancer (all p < 0.05). Using cut-off values of CA15-3, 8-hydroxy-2'-deoxyguanosine, 1-methylguanosine and 1-methyl adenosine of 30.5 U/l, 15.0 µg/l, 18.5 µg/l and 22.0 µg/l, respectively, diagnostic performance analyses of combined metabolome with CA15-3 gave a ROCC area under the curve of 0.94 (95% CI: 0.91-0.98)(p < 0.01) with good sensitivity (88.8%), specificity (86.8%) and efficiency (90.6%). Unlike CA15.3, the highest levels of each of the metabolite were in the early stage of breast cancer.</p><p><strong>Conclusion: </strong>The diagnostic combination test of candidate metabolome with CA15.3 may be a useful tool for the early detection of breast cancer and used as a metabolomics signature in this disease.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"77 4","pages":"196-201"},"PeriodicalIF":1.9,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1784568","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38051121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"British Journal of Biomedical Science in 2020. What have we learned?","authors":"N W Brown,&nbsp;G Orchard,&nbsp;A Rhodes","doi":"10.1080/09674845.2020.1827578","DOIUrl":"https://doi.org/10.1080/09674845.2020.1827578","url":null,"abstract":"<p><p>Each year the British Journal of Biomedical Science publishes a 'What have we learned' editorial designed to introduce readers within the major disciplines of laboratory medicine to developments outside their immediate area. In addition it is designed to inform a wider readership of the advances in the diagnosis and treatment of disease. To this end, in 2020 the journal published 39 articles covering the disciplines within Biomedical Science in the 4 issues comprising volume 77. These included a review of COVID-19 in this issue, 27 original articles, 6 Biomedical Science 'In Brief' and 4 case histories. 27 of the articles involved molecular techniques, with one of these comparing results with a mass spectrometry based method. The preponderance of molecular genetic studies gives us a good idea of the likely future direction of the disciplines.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"77 4","pages":"159-167"},"PeriodicalIF":1.9,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1827578","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38653560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nicotinic acetylcholine receptors (rs1051730) gene polymorphism and surfactant protein D level in chronic obstructive pulmonary disease.","authors":"Ema El Gayed,&nbsp;R H Elhelbawy,&nbsp;N G Elhelbawy","doi":"10.1080/09674845.2020.1757839","DOIUrl":"https://doi.org/10.1080/09674845.2020.1757839","url":null,"abstract":"Chronic obstructive pulmonary disease (COPD) is a complicated disorder that may be genetically determined. It is possible that genetic determinants of nicotine addiction contribute to COPD burden. Surfactant protein D (SP-D) ensures correct ventilatory function . The aim of this work is to study serum level of (SP-D) and nicotinic acetylcholine receptors (nAChRs) (rs1051730) (G/A) gene polymorphism in COPD. We recruited 80 patients with COPD and 80 ages and gender matched healthy individuals. Estimation of Serum SP-D level (by ELISA) and genotyping of (nAChRs) (rs1051730) using TaqMan Allelic discrimination assay technique (real time PCR) were done for all individuals. Results showed significant increased serum SP-D level (P<0.001) and AA genotype and A allele frequencies in COPD patients (P< 0.001) with OR (CI) [6.15 (2.76-13.72) and 3.65 (2.29-5.78) respectively]. We conclude that AA genotype of nAChRs (rs1051730) and elevated serum level of SP-D might be risk factors for COPD.","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"77 4","pages":"213-215"},"PeriodicalIF":1.9,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1757839","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37841371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}